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The map label for this gene is dosC [H]

Identifier: 153935993

GI number: 153935993

Start: 113629

End: 114699

Strand: Direct

Name: dosC [H]

Synonym: CLC_0139

Alternate gene names: 153935993

Gene position: 113629-114699 (Clockwise)

Preceding gene: 153935054

Following gene: 153935494

Centisome position: 3.02

GC content: 19.33

Gene sequence:

>1071_bases
ATGTTATATGTTCTTATAATTACATTATTTTTAATTATGATAGTATATGAAAGTATCATCATAATTAATCTAAGAAAAAA
CATAAAAAAGTTAGAATATTCTAAGAGAATGATCTATAACATGACGAAAAATGTTAATAAAGAAAAAACAATAGACAATG
TATATGAAATAATGTTAAAAACAGCTATAAATCTAATACCAAAAGCAACTCAAGGTAGTATTTTGACAATGGATAAGGAA
GAAAACTTTAATTATGTAGCAATACTAGGATATGATAATAAGTTAAAAAATATAAAACTAAACAAAAAAGAAGTTTATTT
ATATAAAATAAATAGATTTAAGGAAACGGCCATAATTGAAAATCCAATAAAATTAGATGAAAAAATTTTAAATAAAGAAA
AAATAAATGAATTTAAAGAGTTAGAAGCATTAAATATAAGTTGTATAATTTCAGCACCAATATATATAGATAATAAATTA
TTTGGCCAAATCAATATAGATTGTACAAAAGAAAATTATAAATTTAAAAAAGAAGATTTAGAGTTAATGGATCATATAAA
AGATGAATTACAATTAATAATAAAATCTTTTATGGTAAAAGAAGAACTAATACATAAAGCTAATTATGATGAATTGACAA
AAATTTTTAATAGAAGATATTTTAATGAAATAATGAAAAACGAAATAAAAGAAGGTAATAAGATTTTAGTACTAATTGAT
ATTGACAATTTCAAAACTATAAATGATATTTATGGACATAGTACTGGAGATTTTATTTTAAGGGTTTTTGCTAATTTAAT
AAAAAGATACTTAGGAGATAAAGATTTATTTTTTAGATTTGGTGGTGATGAGTTTATAGTATTATTTGATGACTTAGATG
ATAAAAGTGTTATAAGTTTTATGGAAAATATTAGACAAAAAATTAATAAAGATAAAATTAAAGGAATAAATATAGATTTT
AGCTATGGTATAAGTAAACTTAAAAACTATAATATGACAAACTATGAGGATATAATAAATTTAGCTGATAAAAATATGTA
TATTAATAAGAATAAGAAAATTATAGTATAA

Upstream 100 bases:

>100_bases
ATGTCTATAATTTCACCTAACATAGTAGCACCTCCTTTATATATTTTTTTATAAAAGACATATATTATTCATTAATTAAA
TAAATTAATTGGGGGTTAGT

Downstream 100 bases:

>100_bases
AAATAGTAACCTGTTAAAGCTGTTAGCATATATTCATAGATATAGAGGTGAATATATTGGATAGAAAACAGGTGGTATTT
AGAATATTTTTATCTATAGT

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDNVYEIMLKTAINLIPKATQGSILTMDKE
ENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKILVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFDDLDDKSVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV

Sequences:

>Translated_356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDNVYEIMLKTAINLIPKATQGSILTMDKE
ENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKILVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFDDLDDKSVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
>Mature_356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDNVYEIMLKTAINLIPKATQGSILTMDKE
ENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKILVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFDDLDDKSVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=35.8974358974359, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081881, Length=158, Percent_Identity=32.9113924050633, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786584, Length=146, Percent_Identity=30.8219178082192, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=27.906976744186, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787262, Length=178, Percent_Identity=30.8988764044944, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787802, Length=154, Percent_Identity=33.1168831168831, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=153, Percent_Identity=30.0653594771242, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=191, Percent_Identity=30.3664921465969, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=29.8245614035088, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=349, Percent_Identity=22.3495702005731, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=27.5167785234899, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=146, Percent_Identity=32.8767123287671, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=31.6455696202532, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42222; Mature: 42222

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDNVYEIMLK
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAINLIPKATQGSILTMDKEENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIE
HHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEECCCEEEEEEECCHHHHHHCC
NPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKLFGQINIDCTKENYKFKKEDL
CCHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCEEECCEEEEEEEEEEECCCCCCCHHHH
ELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKILVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFDDLDDKSVISF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCHHHHHH
MENIRQKINKDKIKGINIDFSYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
HHHHHHHHCCCCCCEEEEEHHHCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCEEC
>Mature Secondary Structure
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDNVYEIMLK
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAINLIPKATQGSILTMDKEENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIE
HHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEECCCEEEEEEECCHHHHHHCC
NPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKLFGQINIDCTKENYKFKKEDL
CCHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCEEECCEEEEEEEEEEECCCCCCCHHHH
ELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKILVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFDDLDDKSVISF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCHHHHHH
MENIRQKINKDKIKGINIDFSYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
HHHHHHHHCCCCCCEEEEEHHHCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]