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The map label for this gene is cobQ

Identifier: 153935702

GI number: 153935702

Start: 992769

End: 994250

Strand: Direct

Name: cobQ

Synonym: CLC_0968

Alternate gene names: 153935702

Gene position: 992769-994250 (Clockwise)

Preceding gene: 153934718

Following gene: 153936192

Centisome position: 26.4

GC content: 30.97

Gene sequence:

>1482_bases
ATGGCTAAAATAATGATTCAGGGAACGGCATCTTCAGTAGGAAAAAGTCTTATAGTGGCTGCCCTTTGTAGAATATTTAA
ACAGGATGGATATAGCGTTTGTCCTTTTAAATCACAAAATATGTCTTTAAATTCTTATATAACTTTAGATGGTAAGGAAA
TGGGGAGGGCTCAAGTACTTCAGGCTTATGCAGCTGGTTTAGAACCAGAGGCTTATATGAATCCTATACTTTTAAAGCCT
ACTTCAGATAAAAAATCCCAAATAATAGTTAATGGTAAAGTATACGGAAATAGTACAGCTATGGAATATCATAATTTAAA
GATAAAATTTAAAGACATGTTAAAAGAGCAGTTCGAAAAATTAGAAGAAGATTTTGACATAGTTGTTATGGAGGGGGCAG
GAAGTCCAGCAGAAATAAATTTAAGAGATAGAGATATAGTAAACATGGGAATGGCAGAACTTGTGGATGCTCCTGTACTT
TTAGTTGGAGATATAGATAAAGGAGGAGTTTTTGCATCTTTAGCAGGAACTATGCTTCTTTTGAATGAAGGAGAAAAGGA
AAGGGTAAAAGGAACTATAATAAACAAGTTTAGAGGAGATGTAGAAATACTAAAACCAGGTTTAGATATGCTAGAAGATA
TAATACATATACCTTGCTTAGGGGTAGTACCTTATACTAGATTACAGTTAGAAGATGAGGATGGAGCAGTGGAATTTAAT
AAAAAAGCTTATGCACCAATCGACATAGCCGTAATAAAAATGCCTCATATATCAAATTTTACAGATTTGGATGCTTTAAA
GTCAGAAGAGGATGTATCTATAAGATTTGTAACTTCAAAAGAGGAATTTAAAGAGCCAGATCTATTAATAATACCAGGAA
GTAAAAATACCATAGAGGATTTACTTTATTTAAGACAATGTGGATTAGAAGAACGTATAAAAGAATATAGTAGAGAGGGC
AAAATAATAGGTATCTGTGGAGGATATCAAGTATTAGGTAGTAAAATTAAAGATCCATACAAAGTGGAGACAGATTTAGG
AGAGATTGATGGATTAAATCTTTTAGATATGGAAACTACCTTTGAAAAAGAAAAGGTTACTACACGAGTTTCTGCAAAAC
TTTTAAATGAAGAAACTAAAAATACTGTTTATGGTTATGAAATACATATGGGAATAAGTAAGTATGGCGAAAATATCAAA
CCATTATTTAAAATCTATGATAAAAATGGAGAAAAAGTAGATTATTTTGATGGAGCCATAAATGAAAAAGGAAATGTAAT
GGGAACTTATATCCATGGAGTTTTTGATGGAGTAGTTTTTAGGGAAAAAATAATAAATGAGTTAAGAGTTAAAAAAGGGT
TAAAAAAGAAAAAATCACAAATGTATGAACATATGAGAGAAAAAGAGTTAGATAAGCTTGCGGATATAGTAAGGCAAAGC
TTAGATATGGAAAAAATTTATTCTATTATAGGGATGAAATAA

Upstream 100 bases:

>100_bases
AGGCGTAGTATGCCTATTTTTAACAATGCATTGGCTATAAACTCTAAAGTTTATAGCCAATTTTTATATTGAAAAATAAA
TTGAAATTGGAGAGAATTTT

Downstream 100 bases:

>100_bases
GATGTATTTAAATATAACTACAAATTTTATAGATATAATTATAGCGGTTTTTATAGATTGGATTATTGGAGATCCTTATT
GGTTTCCCCATCCAGTTATA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAKIMIQGTASSVGKSLIVAALCRIFKQDGYSVCPFKSQNMSLNSYITLDGKEMGRAQVLQAYAAGLEPEAYMNPILLKP
TSDKKSQIIVNGKVYGNSTAMEYHNLKIKFKDMLKEQFEKLEEDFDIVVMEGAGSPAEINLRDRDIVNMGMAELVDAPVL
LVGDIDKGGVFASLAGTMLLLNEGEKERVKGTIINKFRGDVEILKPGLDMLEDIIHIPCLGVVPYTRLQLEDEDGAVEFN
KKAYAPIDIAVIKMPHISNFTDLDALKSEEDVSIRFVTSKEEFKEPDLLIIPGSKNTIEDLLYLRQCGLEERIKEYSREG
KIIGICGGYQVLGSKIKDPYKVETDLGEIDGLNLLDMETTFEKEKVTTRVSAKLLNEETKNTVYGYEIHMGISKYGENIK
PLFKIYDKNGEKVDYFDGAINEKGNVMGTYIHGVFDGVVFREKIINELRVKKGLKKKKSQMYEHMREKELDKLADIVRQS
LDMEKIYSIIGMK

Sequences:

>Translated_493_residues
MAKIMIQGTASSVGKSLIVAALCRIFKQDGYSVCPFKSQNMSLNSYITLDGKEMGRAQVLQAYAAGLEPEAYMNPILLKP
TSDKKSQIIVNGKVYGNSTAMEYHNLKIKFKDMLKEQFEKLEEDFDIVVMEGAGSPAEINLRDRDIVNMGMAELVDAPVL
LVGDIDKGGVFASLAGTMLLLNEGEKERVKGTIINKFRGDVEILKPGLDMLEDIIHIPCLGVVPYTRLQLEDEDGAVEFN
KKAYAPIDIAVIKMPHISNFTDLDALKSEEDVSIRFVTSKEEFKEPDLLIIPGSKNTIEDLLYLRQCGLEERIKEYSREG
KIIGICGGYQVLGSKIKDPYKVETDLGEIDGLNLLDMETTFEKEKVTTRVSAKLLNEETKNTVYGYEIHMGISKYGENIK
PLFKIYDKNGEKVDYFDGAINEKGNVMGTYIHGVFDGVVFREKIINELRVKKGLKKKKSQMYEHMREKELDKLADIVRQS
LDMEKIYSIIGMK
>Mature_492_residues
AKIMIQGTASSVGKSLIVAALCRIFKQDGYSVCPFKSQNMSLNSYITLDGKEMGRAQVLQAYAAGLEPEAYMNPILLKPT
SDKKSQIIVNGKVYGNSTAMEYHNLKIKFKDMLKEQFEKLEEDFDIVVMEGAGSPAEINLRDRDIVNMGMAELVDAPVLL
VGDIDKGGVFASLAGTMLLLNEGEKERVKGTIINKFRGDVEILKPGLDMLEDIIHIPCLGVVPYTRLQLEDEDGAVEFNK
KAYAPIDIAVIKMPHISNFTDLDALKSEEDVSIRFVTSKEEFKEPDLLIIPGSKNTIEDLLYLRQCGLEERIKEYSREGK
IIGICGGYQVLGSKIKDPYKVETDLGEIDGLNLLDMETTFEKEKVTTRVSAKLLNEETKNTVYGYEIHMGISKYGENIKP
LFKIYDKNGEKVDYFDGAINEKGNVMGTYIHGVFDGVVFREKIINELRVKKGLKKKKSQMYEHMREKELDKLADIVRQSL
DMEKIYSIIGMK

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_CLOB1 (A7FSG1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383288.1
- ProteinModelPortal:   A7FSG1
- SMR:   A7FSG1
- STRING:   A7FSG1
- GeneID:   5395718
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0954
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   CBOT441770:CLB_0954-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 55415; Mature: 55284

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 326-326 ACT_SITE 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIMIQGTASSVGKSLIVAALCRIFKQDGYSVCPFKSQNMSLNSYITLDGKEMGRAQVL
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCHHCCHHHHH
QAYAAGLEPEAYMNPILLKPTSDKKSQIIVNGKVYGNSTAMEYHNLKIKFKDMLKEQFEK
HHHHCCCCCHHHCCCEEEECCCCCCCEEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHH
LEEDFDIVVMEGAGSPAEINLRDRDIVNMGMAELVDAPVLLVGDIDKGGVFASLAGTMLL
HCCCCCEEEEECCCCCCEEEECCCHHHHCCHHHHCCCCEEEEECCCCCCCHHHHHCEEEE
LNEGEKERVKGTIINKFRGDVEILKPGLDMLEDIIHIPCLGVVPYTRLQLEDEDGAVEFN
ECCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEC
KKAYAPIDIAVIKMPHISNFTDLDALKSEEDVSIRFVTSKEEFKEPDLLIIPGSKNTIED
CCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCEEEEEECHHHHCCCCEEEECCCCCHHHH
LLYLRQCGLEERIKEYSREGKIIGICGGYQVLGSKIKDPYKVETDLGEIDGLNLLDMETT
HHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCEEEECCCCCCCCCEEEECHHH
FEKEKVTTRVSAKLLNEETKNTVYGYEIHMGISKYGENIKPLFKIYDKNGEKVDYFDGAI
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHCCCCCEEHHEECCCCCEEEEECCCC
NEKGNVMGTYIHGVFDGVVFREKIINELRVKKGLKKKKSQMYEHMREKELDKLADIVRQS
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDMEKIYSIIGMK
CCHHHHHHHHCCC
>Mature Secondary Structure 
AKIMIQGTASSVGKSLIVAALCRIFKQDGYSVCPFKSQNMSLNSYITLDGKEMGRAQVL
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCHHCCHHHHH
QAYAAGLEPEAYMNPILLKPTSDKKSQIIVNGKVYGNSTAMEYHNLKIKFKDMLKEQFEK
HHHHCCCCCHHHCCCEEEECCCCCCCEEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHH
LEEDFDIVVMEGAGSPAEINLRDRDIVNMGMAELVDAPVLLVGDIDKGGVFASLAGTMLL
HCCCCCEEEEECCCCCCEEEECCCHHHHCCHHHHCCCCEEEEECCCCCCCHHHHHCEEEE
LNEGEKERVKGTIINKFRGDVEILKPGLDMLEDIIHIPCLGVVPYTRLQLEDEDGAVEFN
ECCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEC
KKAYAPIDIAVIKMPHISNFTDLDALKSEEDVSIRFVTSKEEFKEPDLLIIPGSKNTIED
CCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCEEEEEECHHHHCCCCEEEECCCCCHHHH
LLYLRQCGLEERIKEYSREGKIIGICGGYQVLGSKIKDPYKVETDLGEIDGLNLLDMETT
HHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCEEEECCCCCCCCCEEEECHHH
FEKEKVTTRVSAKLLNEETKNTVYGYEIHMGISKYGENIKPLFKIYDKNGEKVDYFDGAI
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHCCCCCEEHHEECCCCCEEEEECCCC
NEKGNVMGTYIHGVFDGVVFREKIINELRVKKGLKKKKSQMYEHMREKELDKLADIVRQS
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDMEKIYSIIGMK
CCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA