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The map label for this gene is pbpA

Identifier: 153935583

GI number: 153935583

Start: 3138954

End: 3141446

Strand: Direct

Name: pbpA

Synonym: CLC_2985

Alternate gene names: 153935583

Gene position: 3138954-3141446 (Clockwise)

Preceding gene: 153935027

Following gene: 153935641

Centisome position: 83.47

GC content: 30.81

Gene sequence:

>2493_bases
ATGGGAAAAAAGAAAAAAAAGCGCAAATCTAGCGCCTTTAAAATAATACTTAATGTCTTTTTAAGTATTTTCTTAGTAGC
TGGAGTAGCCTTTGGTGGAATTGTGTTTGCTATGATAAAAACAGCTCCTCCACTAAATGTACAGCAAGTTCTCACTTTTG
ATGAACCCTCTATACTTTATGATGATAAAGGTCAATATATGGATAAAGTTATAACTAACGAACAAAGAATTGTAGTTGAT
TATAAAAATGTACCCCAAAATTTAAAAAATGCATTTGTAAGTATAGAAGATGAAAGATTTTATAAACATCATGGAGTTGA
CATAAAAAGATTTACTGGTGTTATATTAATTAATGTGACTAATAAAATAAAAAGGAGTTCTAAACTTCAAGGAGCTTCTA
CTCTAACTCAACAGTTAATAAAAAATACAGTTTTATCCTCTGAAGTTTCTATTAAAAGAAAAGTTCAAGAAATGTACTTA
TCTATACAACTAGAAAAAGAACTATCAAAGGATGAAATTTTGGGTGCCTATATGAATAGTATATTTTTAGGCGGAAATGC
TTTAGGAGTAGAGGCAGCATCAAAACAGTATTTTAATAAAAGTGTAAAAGATCTATCTTTAATAGAATGTGCTTTTATTG
CTGGAGTACCTCAAAGTCCATCAGTTTACTATCCCTATTCATCTGCTTCTAAGAAAAACCCTTCTATTTATTTAAATAGA
ACAAAAACTGTTTTATATAAAATGTTAGACAATGGCTATATTACTCAAAATGATTATAATAAAGCTTTAAAGGATTTAGA
TAGTAAAAAGCTAGTCTTTGCTAAACCATCTGCCCCTAGCAATAAGTTAGCCTATGAATGGTTCTCTATTCCTGCTATTG
AGCAAGTAAAAAAAGATTTAAAGACTCAATATAAATATGATGACAAACAAATTCATAACCTTTTAGTTAATGGTGGCTTA
AAAGTATATACTACTATGAATAAAAATTTACAAGATAAAACTCAAAATACAATAAATAATGCCTATTATTTAAATTCTTA
TAAGAGTAATGGTATGATATATCCTCAAGCCTCTGCTGTAATAATGGACTATCATAATGGTGAGGTAAAAACTATAATTG
GAGGTAGGGGCGACCAACCTGCTAGATCTTATAACAGAGCAGCCTCTTATAACTATCTAAGACCTGCCGGTTCTAGTATT
AAACCTCTTACAGTGTATAGTGCTGCTATAGATAGTAAAAAAGCCACTGCTGCTACTGGATTTGAAGATTCTCCAATTCC
AAATAATATAGGACGTAAATATAGTTCTGGAGCACCTTATAATCCTAAAAATAGCCCTGATATTTATTATGGATATGTAA
ATGTAAGAGAGGCCTTAATGCGTTCTATAAATGTAGTAGCTGTAAAACTTGTAGATAAGATAGGACTAAATACTAGTATA
CAATATGCTGAAAAATTTGGAATTCCTATAGATCAACACGATAGATCTAGTATAGCATCTCTATCTTTGGGAGAATTACA
TAAAGGTACTAATCCTTTAATAATGGCTCAAGCCTACGGTGTATTTGGCAATAATGGAACCTACACAGAAGCAAAGTTAT
ATACAAAAGTAGTAGATAGAACAGGTAAAGTTCTTTTAGAACCTAAAACTAATACAAAAAAAGTTCTTTCACCAGAAGCA
GCCTTTATAACTTATGATATGCTTCAAGGTCCTGTAAGCGAAAGTGGTACTGGACCTCAAGCCAATTTTGGTAATATGGA
AGTTAGAGGTAAGACTGGTACATCTAGTGATATGAAAAATTTATGGTTCTGTGGATTAACTCCTTATTATTCCGCAGCTG
TTTGGATAGGTAATGACAATTCTTCTACAGTGGATGGGGTATATAGTAGTACTGCAGCTAGACTTTGGGGAGATATTATG
AAAGAATTTCATGTTAATCTTCCTTATAAACAAGTTCAAAAACCAGCTAGTGTTGTAACTGCTAATGTCGATAGAATTTC
TGGTAAACTTCCAACTCAGCTATCTTATAGAGATCCTAGAGGTAGTACAGTATATAATGAATTTTTTATAAATGGAACTA
TTCCAACAGAATATGATGATATACATGTAGAAGCTCAAATAAATAAGTTAACTGGTAAATTAGCAAGTAAATTCACCCCT
TCATTCCTTGTAGAATCTAGGGTATTCTTAAGAAGAGATTACTCTCCTGGTGTAGAACTTTTAGATCAACAATGGCTTCT
CCCTTATTCTATAGACGAAGGAGGAAGCTTACCACCTACTGAAGAAAAAAATAACTCTAATACTAGAGATAAAAATAAAG
ATAAGAATAAAAATAAGAATAAAGATAAGAATCCTTCACAGGATAAACCTAATAATAATAACAATGATAACAATAGTAAC
AACAATAATAACAATAACGACAATAACAACAATACTAAACCTCCTGAAAATGATTCAAATCAAAATCACGAAGACAATAA
AAACAAACAATAA

Upstream 100 bases:

>100_bases
ATAATTAGGATTTTTTCTATTTATTCATAAGTAATTCAATATGTGTTATAATATTAAAAATAATGTTTTATTTTACCCTA
ACTAAGGAGGACAAATATAT

Downstream 100 bases:

>100_bases
AAAGAATAAAAACTCCTTCTGGGGAGTTTTTATTCTTTTGTTTTAGGATTAAATATTAAAGCCATTATATATCCGAATAT
AATAGCCGCAGTTATTCCTC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase

Number of amino acids: Translated: 830; Mature: 829

Protein sequence:

>830_residues
MGKKKKKRKSSAFKIILNVFLSIFLVAGVAFGGIVFAMIKTAPPLNVQQVLTFDEPSILYDDKGQYMDKVITNEQRIVVD
YKNVPQNLKNAFVSIEDERFYKHHGVDIKRFTGVILINVTNKIKRSSKLQGASTLTQQLIKNTVLSSEVSIKRKVQEMYL
SIQLEKELSKDEILGAYMNSIFLGGNALGVEAASKQYFNKSVKDLSLIECAFIAGVPQSPSVYYPYSSASKKNPSIYLNR
TKTVLYKMLDNGYITQNDYNKALKDLDSKKLVFAKPSAPSNKLAYEWFSIPAIEQVKKDLKTQYKYDDKQIHNLLVNGGL
KVYTTMNKNLQDKTQNTINNAYYLNSYKSNGMIYPQASAVIMDYHNGEVKTIIGGRGDQPARSYNRAASYNYLRPAGSSI
KPLTVYSAAIDSKKATAATGFEDSPIPNNIGRKYSSGAPYNPKNSPDIYYGYVNVREALMRSINVVAVKLVDKIGLNTSI
QYAEKFGIPIDQHDRSSIASLSLGELHKGTNPLIMAQAYGVFGNNGTYTEAKLYTKVVDRTGKVLLEPKTNTKKVLSPEA
AFITYDMLQGPVSESGTGPQANFGNMEVRGKTGTSSDMKNLWFCGLTPYYSAAVWIGNDNSSTVDGVYSSTAARLWGDIM
KEFHVNLPYKQVQKPASVVTANVDRISGKLPTQLSYRDPRGSTVYNEFFINGTIPTEYDDIHVEAQINKLTGKLASKFTP
SFLVESRVFLRRDYSPGVELLDQQWLLPYSIDEGGSLPPTEEKNNSNTRDKNKDKNKNKNKDKNPSQDKPNNNNNDNNSN
NNNNNNDNNNNTKPPENDSNQNHEDNKNKQ

Sequences:

>Translated_830_residues
MGKKKKKRKSSAFKIILNVFLSIFLVAGVAFGGIVFAMIKTAPPLNVQQVLTFDEPSILYDDKGQYMDKVITNEQRIVVD
YKNVPQNLKNAFVSIEDERFYKHHGVDIKRFTGVILINVTNKIKRSSKLQGASTLTQQLIKNTVLSSEVSIKRKVQEMYL
SIQLEKELSKDEILGAYMNSIFLGGNALGVEAASKQYFNKSVKDLSLIECAFIAGVPQSPSVYYPYSSASKKNPSIYLNR
TKTVLYKMLDNGYITQNDYNKALKDLDSKKLVFAKPSAPSNKLAYEWFSIPAIEQVKKDLKTQYKYDDKQIHNLLVNGGL
KVYTTMNKNLQDKTQNTINNAYYLNSYKSNGMIYPQASAVIMDYHNGEVKTIIGGRGDQPARSYNRAASYNYLRPAGSSI
KPLTVYSAAIDSKKATAATGFEDSPIPNNIGRKYSSGAPYNPKNSPDIYYGYVNVREALMRSINVVAVKLVDKIGLNTSI
QYAEKFGIPIDQHDRSSIASLSLGELHKGTNPLIMAQAYGVFGNNGTYTEAKLYTKVVDRTGKVLLEPKTNTKKVLSPEA
AFITYDMLQGPVSESGTGPQANFGNMEVRGKTGTSSDMKNLWFCGLTPYYSAAVWIGNDNSSTVDGVYSSTAARLWGDIM
KEFHVNLPYKQVQKPASVVTANVDRISGKLPTQLSYRDPRGSTVYNEFFINGTIPTEYDDIHVEAQINKLTGKLASKFTP
SFLVESRVFLRRDYSPGVELLDQQWLLPYSIDEGGSLPPTEEKNNSNTRDKNKDKNKNKNKDKNPSQDKPNNNNNDNNSN
NNNNNNDNNNNTKPPENDSNQNHEDNKNKQ
>Mature_829_residues
GKKKKKRKSSAFKIILNVFLSIFLVAGVAFGGIVFAMIKTAPPLNVQQVLTFDEPSILYDDKGQYMDKVITNEQRIVVDY
KNVPQNLKNAFVSIEDERFYKHHGVDIKRFTGVILINVTNKIKRSSKLQGASTLTQQLIKNTVLSSEVSIKRKVQEMYLS
IQLEKELSKDEILGAYMNSIFLGGNALGVEAASKQYFNKSVKDLSLIECAFIAGVPQSPSVYYPYSSASKKNPSIYLNRT
KTVLYKMLDNGYITQNDYNKALKDLDSKKLVFAKPSAPSNKLAYEWFSIPAIEQVKKDLKTQYKYDDKQIHNLLVNGGLK
VYTTMNKNLQDKTQNTINNAYYLNSYKSNGMIYPQASAVIMDYHNGEVKTIIGGRGDQPARSYNRAASYNYLRPAGSSIK
PLTVYSAAIDSKKATAATGFEDSPIPNNIGRKYSSGAPYNPKNSPDIYYGYVNVREALMRSINVVAVKLVDKIGLNTSIQ
YAEKFGIPIDQHDRSSIASLSLGELHKGTNPLIMAQAYGVFGNNGTYTEAKLYTKVVDRTGKVLLEPKTNTKKVLSPEAA
FITYDMLQGPVSESGTGPQANFGNMEVRGKTGTSSDMKNLWFCGLTPYYSAAVWIGNDNSSTVDGVYSSTAARLWGDIMK
EFHVNLPYKQVQKPASVVTANVDRISGKLPTQLSYRDPRGSTVYNEFFINGTIPTEYDDIHVEAQINKLTGKLASKFTPS
FLVESRVFLRRDYSPGVELLDQQWLLPYSIDEGGSLPPTEEKNNSNTRDKNKDKNKNKNKDKNPSQDKPNNNNNDNNSNN
NNNNNDNNNNTKPPENDSNQNHEDNKNKQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786343, Length=559, Percent_Identity=30.5903398926655, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI87082258, Length=336, Percent_Identity=32.7380952380952, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI1788867, Length=563, Percent_Identity=23.9786856127886, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789601, Length=163, Percent_Identity=33.1288343558282, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPA_CLOB1 (A7FY32)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385405.1
- ProteinModelPortal:   A7FY32
- SMR:   A7FY32
- STRING:   A7FY32
- GeneID:   5395318
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3112
- eggNOG:   COG0744
- HOGENOM:   HBG672432
- OMA:   QNTINDA
- ProtClustDB:   CLSK883109
- BioCyc:   CBOT441770:CLB_3112-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460
- TIGRFAMs:   TIGR02074

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 92705; Mature: 92573

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: ACT_SITE 398-398

Signals:

None

Transmembrane regions:

HASH(0x1b786608)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKKKKRKSSAFKIILNVFLSIFLVAGVAFGGIVFAMIKTAPPLNVQQVLTFDEPSILY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCEEE
DDKGQYMDKVITNEQRIVVDYKNVPQNLKNAFVSIEDERFYKHHGVDIKRFTGVILINVT
CCCHHHHHHHHCCCCEEEEECCCCCHHHHHHEEEECHHHHHHHCCCCHHHCCCEEEEEEC
NKIKRSSKLQGASTLTQQLIKNTVLSSEVSIKRKVQEMYLSIQLEKELSKDEILGAYMNS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCHHHHHHHHHHH
IFLGGNALGVEAASKQYFNKSVKDLSLIECAFIAGVPQSPSVYYPYSSASKKNPSIYLNR
EEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEH
TKTVLYKMLDNGYITQNDYNKALKDLDSKKLVFAKPSAPSNKLAYEWFSIPAIEQVKKDL
HHHHHHHHHCCCEEECCHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHH
KTQYKYDDKQIHNLLVNGGLKVYTTMNKNLQDKTQNTINNAYYLNSYKSNGMIYPQASAV
HHHCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCEEEECCCEE
IMDYHNGEVKTIIGGRGDQPARSYNRAASYNYLRPAGSSIKPLTVYSAAIDSKKATAATG
EEEECCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEECC
FEDSPIPNNIGRKYSSGAPYNPKNSPDIYYGYVNVREALMRSINVVAVKLVDKIGLNTSI
CCCCCCCCHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHEEHHHHHCCCCCH
QYAEKFGIPIDQHDRSSIASLSLGELHKGTNPLIMAQAYGVFGNNGTYTEAKLYTKVVDR
HHHHHCCCCCCCCCCHHHHHCCHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHC
TGKVLLEPKTNTKKVLSPEAAFITYDMLQGPVSESGTGPQANFGNMEVRGKTGTSSDMKN
CCCEEECCCCCCCHHCCCCHHEEEHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
LWFCGLTPYYSAAVWIGNDNSSTVDGVYSSTAARLWGDIMKEFHVNLPYKQVQKPASVVT
EEEECCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHEE
ANVDRISGKLPTQLSYRDPRGSTVYNEFFINGTIPTEYDDIHVEAQINKLTGKLASKFTP
ECHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCH
SFLVESRVFLRRDYSPGVELLDQQWLLPYSIDEGGSLPPTEEKNNSNTRDKNKDKNKNKN
HHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KDKNPSQDKPNNNNNDNNSNNNNNNNDNNNNTKPPENDSNQNHEDNKNKQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
GKKKKKRKSSAFKIILNVFLSIFLVAGVAFGGIVFAMIKTAPPLNVQQVLTFDEPSILY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCEEE
DDKGQYMDKVITNEQRIVVDYKNVPQNLKNAFVSIEDERFYKHHGVDIKRFTGVILINVT
CCCHHHHHHHHCCCCEEEEECCCCCHHHHHHEEEECHHHHHHHCCCCHHHCCCEEEEEEC
NKIKRSSKLQGASTLTQQLIKNTVLSSEVSIKRKVQEMYLSIQLEKELSKDEILGAYMNS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCHHHHHHHHHHH
IFLGGNALGVEAASKQYFNKSVKDLSLIECAFIAGVPQSPSVYYPYSSASKKNPSIYLNR
EEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEH
TKTVLYKMLDNGYITQNDYNKALKDLDSKKLVFAKPSAPSNKLAYEWFSIPAIEQVKKDL
HHHHHHHHHCCCEEECCHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHH
KTQYKYDDKQIHNLLVNGGLKVYTTMNKNLQDKTQNTINNAYYLNSYKSNGMIYPQASAV
HHHCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCEEEECCCEE
IMDYHNGEVKTIIGGRGDQPARSYNRAASYNYLRPAGSSIKPLTVYSAAIDSKKATAATG
EEEECCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEECC
FEDSPIPNNIGRKYSSGAPYNPKNSPDIYYGYVNVREALMRSINVVAVKLVDKIGLNTSI
CCCCCCCCHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHEEHHHHHCCCCCH
QYAEKFGIPIDQHDRSSIASLSLGELHKGTNPLIMAQAYGVFGNNGTYTEAKLYTKVVDR
HHHHHCCCCCCCCCCHHHHHCCHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHC
TGKVLLEPKTNTKKVLSPEAAFITYDMLQGPVSESGTGPQANFGNMEVRGKTGTSSDMKN
CCCEEECCCCCCCHHCCCCHHEEEHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
LWFCGLTPYYSAAVWIGNDNSSTVDGVYSSTAARLWGDIMKEFHVNLPYKQVQKPASVVT
EEEECCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHEE
ANVDRISGKLPTQLSYRDPRGSTVYNEFFINGTIPTEYDDIHVEAQINKLTGKLASKFTP
ECHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCH
SFLVESRVFLRRDYSPGVELLDQQWLLPYSIDEGGSLPPTEEKNNSNTRDKNKDKNKNKN
HHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KDKNPSQDKPNNNNNDNNSNNNNNNNDNNNNTKPPENDSNQNHEDNKNKQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA