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The map label for this gene is dapF [H]

Identifier: 153935384

GI number: 153935384

Start: 1960796

End: 1961617

Strand: Reverse

Name: dapF [H]

Synonym: CLC_1856

Alternate gene names: 153935384

Gene position: 1961617-1960796 (Counterclockwise)

Preceding gene: 153934951

Following gene: 153936505

Centisome position: 52.16

GC content: 32.12

Gene sequence:

>822_bases
ATGAAGTTTACTAAAATGACTGGAACTGGTAATGATTTTATAGTTTTAGATGACAGAAAGGGAAGTTTGATAGGAAAAGA
GAAAGAGTTAGCACAAAAATTATGTAATAGACATTTTAGTGTAGGGGCAGATGGTATTTTATTTGTAAGAGAAAGTGACA
CCGATGCTCAAATACAAATGGTAATAATAAATGCAGATGGTTCTTATGCCTCTATGTGTGGAAATGGTATAAGATGTTTT
GCAAAGTATGTATGGGAAAAATCTATAGTAAAAGAAAATCCTATAATAATAGAAACAGGAGATGGCTTAAAGGAGGCTTT
TCTAGATATTGAAGAGGATAAAGTAAAATATATTACTATTAACATGGGTGAACCAATATTTGAAGGAGAAAAAATATCCA
ATGAAAAGGAAAAAATAATGAATAAAAGTATTAGGGAAAATAACAAAGAATATTCTATAACAGCCTTTCATATGGGAGTT
CCCCATACTATAATATTTGGCAATTTAGATGAATATGATATAGGAGAAGGTAAAAATATAGAAAGGCTACCTTTATTTAA
GGAGGGAACTAATGTAAACTTCTGCGAGGTAGTGGATAAAAATAAAATAAAGGTAAAAACCTGGGAAAGGGGTGCAGGAC
CAACTTTAGCTTGTGGTACTGGAAGTTGTGCATCAGCTATAGCATCTAATTTATTGGGCTATACAGGAAAGTCCACAGAG
GTAATATTGCCAGGGGGCAAACTTTTCATAGAAATTAAAGAAAATAGTGTTTTTATGAAGGGCCCTGCGGATATTTGTTT
TAGAGGAGAAATAGATGTATAA

Upstream 100 bases:

>100_bases
ATTTTTAGGAAATTAAACTAAAATAAAGTTATATTTTATGTAATATTAAAAGCTAATAATAAAAATAATTATAAGTGGTA
ATGTAAAGGATGTGACAATT

Downstream 100 bases:

>100_bases
ATGTTTAAGAAAGGCTTTAAAAATATAGGATATTTTATAATAGTTTTTTTAGTTATTGGCTTAGTTGGGTGTAAAAGCCA
TAGAATATCAGAAGGAGAAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSVGADGILFVRESDTDAQIQMVIINADGSYASMCGNGIRCF
AKYVWEKSIVKENPIIIETGDGLKEAFLDIEEDKVKYITINMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGV
PHTIIFGNLDEYDIGEGKNIERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYTGKSTE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV

Sequences:

>Translated_273_residues
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSVGADGILFVRESDTDAQIQMVIINADGSYASMCGNGIRCF
AKYVWEKSIVKENPIIIETGDGLKEAFLDIEEDKVKYITINMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGV
PHTIIFGNLDEYDIGEGKNIERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYTGKSTE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV
>Mature_273_residues
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSVGADGILFVRESDTDAQIQMVIINADGSYASMCGNGIRCF
AKYVWEKSIVKENPIIIETGDGLKEAFLDIEEDKVKYITINMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGV
PHTIIFGNLDEYDIGEGKNIERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYTGKSTE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=283, Percent_Identity=34.2756183745583, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30217; Mature: 30217

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSVGADGILFVRESDTDAQIQM
CCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEE
VIINADGSYASMCGNGIRCFAKYVWEKSIVKENPIIIETGDGLKEAFLDIEEDKVKYITI
EEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCEEEEEE
NMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGVPHTIIFGNLDEYDIGEGKNI
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCC
ERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYTGKSTE
CCCCCEECCCCCEEEEEECCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCCE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV
EEECCCEEEEEECCCEEEEECCCCEEEECCCCC
>Mature Secondary Structure
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSVGADGILFVRESDTDAQIQM
CCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEE
VIINADGSYASMCGNGIRCFAKYVWEKSIVKENPIIIETGDGLKEAFLDIEEDKVKYITI
EEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCEEEEEE
NMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGVPHTIIFGNLDEYDIGEGKNI
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCC
ERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYTGKSTE
CCCCCEECCCCCEEEEEECCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCCE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV
EEECCCEEEEEECCCEEEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358 [H]