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The map label for this gene is clpB [H]

Identifier: 153935231

GI number: 153935231

Start: 444793

End: 447393

Strand: Direct

Name: clpB [H]

Synonym: CLC_0449

Alternate gene names: 153935231

Gene position: 444793-447393 (Clockwise)

Preceding gene: 153935211

Following gene: 153936302

Centisome position: 11.83

GC content: 30.6

Gene sequence:

>2601_bases
ATGAATATTGAAAAATTAACTTTAAAGGTACAGCAAGCTATAAATGATTCTCAACTTGTTGCAGTTAAGTATAATCATCA
GCAGATTGATACTATACATTTATTTTCAGCCTTAATTTCTCAAGATGATGGATTAATTCCTAATATTTTAGGGAAAATGG
GAGTGAATATAAGAGATATTAATGCTGAAACCAATAGGGTCTTAGATAATATGCCTAAAGTATTAGGTGAGGGAGCACAG
TCATCTTCTGTGTATCCTACTAGAAGGTTTGAAGAGGTTTTTGTAAGGGCAGAACAGATAGCAAAGGATTTTAAAGATTC
ATATATAAGTGTAGAACATGTTTTATTAGCAATAATGGATGTGGATAGAAATACTGTTCTTCCTATATTAGAAAAATTTG
GAGTAAAAAAATCAGAGTTTTTAAAGGCATTATCAGCTGTTAGAGGAAGTCAAAGAGTTGATAATCAAGATCCAGAGGGG
ACTTATGAAGCTTTGGTTAAGTATGGTAGAAATTTAGTTGAAGAAGCTAAGAAACATAAATTGGATCCTGTTATAGGTAG
AGATGAAGAAATAAGAAGAGTTGTAAGAATTTTATCTAGAAGAACTAAGAATAATCCTGTTCTTATTGGAGAACCAGGTG
TAGGTAAGACTGCTATTGTAGAGGGATTAGCAGAGAGAATTGTTAGAGGAGATGTACCAGAAAGTTTAAAAAATAAAATA
ATATTTTCTCTAGATATGGGAGCACTTATTGCAGGTGCAAAATATAGAGGAGAATTTGAAGAAAGATTAAAAGCAGTATT
AAAAGAAGTTCAAAATAGTGAAGGAAGAATAATTTTATTTATAGATGAAATTCATACTATAGTAGGGGCAGGAAAAACAG
AGGGTTCTATGGATGCAGGAAATTTAATAAAACCTATGCTTGCCAGAGGAGAACTTCATTGTATTGGAGCTACTACCTTT
GATGAATATAGACAGTATATAGAAAAAGACAAAGCTTTAGAAAGAAGATTTCAACCTGTAATAACAGATGAGCCTACAGT
AGAGGATACAGTATCTATATTAAGAGGGCTTAAAGAAAGATTTGAAATATATCATGGAATAAGAATACATGATTCAGCTA
TTGTAGCTGCAGCTAAGCTCTCTAGTAGATATATTACAGATAGATATCTCCCAGACAAAGCAATAGATTTAATAGATGAA
GCTTGTGCTATGATAAGAACAGATATAGATAGTATGCCAACGGAAATGGATTCAATGAAAAGAAAAATATTTCAATTAGA
AATAGAAAAGGAAGCATTGTCTAAAGAAAAAGATGTGGCTTCAAAGGAAAGATTAGAAGTTTTAGAAAAGGAATTGAGTG
AACTAAAAGAGAAAGATAAAGAAATGACAGCCAAGTATGAAAATGAAAAATCTCATATAACAAAAATAAGAGATTTAAAA
GAAAAATTGGATGATGTTAGAGGTCAGATGGAAAAAGCCGAAAGAGAATATGATTTGAATAAAGTTGCAGAATTAAAATA
TGGTTTAATACCAGCTCTTCAAAGAGAAATAGAAGAAAAAGAAAAATTAATAAAGGAAAATAGTAACGGAAATGCTATGT
TAAAAGAAGAGGTTACAGAGCAAGAAATTTCTGAAATAATCTCTAAATGGACTGGTATACCTGTAACAAGATTAGTTGAA
ACTGAAAGACAAAAACTATTACAATTAGGAGATCAATTAGAAACTAGAGTAATAGGTCAAGGAGAGGCAGTAAAAGCAGT
AACTAATGCAGTTATAAGAGCTAGAGCAGGACTAAAAGATCCAAGAAAACCTATAGGATCATTTATATTCTTAGGACCTA
CAGGTGTAGGAAAGACAGAACTTGCAAAAACTTTGGCCAGAACTTTATTTGATACTGAAGAAAATATTATAAGAATAGAT
ATGTCTGAATATATGGAAAAATATTCAGTATCAAGACTTATAGGAGCTCCTCCAGGATATGTAGGATATGAAGAAGGAGG
CCAGCTAACAGAAGCCGTTAGGAGAAAACCTTATAGTGTTATATTATTTGATGAAATAGAAAAAGCTCATGATGACGTAT
TTAATATATTCCTTCAAATATTAGATGATGGAAGACTTACGGACAATAAAGGGAAGGTTATAGATTTTAAAAATACTATA
ATTATAATGACTTCTAATTTGGGAAGTAACTATTTATTAGATAATGAAAGCAAAGAAGGTATAGATGAAAGTATAAGAAC
AAGAGTAAAAGAGGCTTTAAAGGCTAGATTTAAACCAGAATTTTTAAATAGAGTAGATGACATTATAATGTTTAAACCTC
TTACGGTAGAAGACATTAAGAGAATAATAGATATATTCTTAGATGATATAAGAAAAAGACTTAAAGATAAGAATATACAG
CTTAAAATTACTGATAGTGCAAAAGATATAATGGCCAAAGAAGGTTATGATCCAATATATGGCGCAAGGCCGCTAAAGAG
ATATATAGAAGATACTATAGAAACAGAAATAGCAAAACAAATAATAGCAGGTAATATATATGAAGGTACTACTGTAGGAG
TAGACTTAAAAGGAGAGTCAATAGTTATAGAAAGAATATAA

Upstream 100 bases:

>100_bases
AATCTATGCATATTAAATTAAATTTTGAAAATATTTAAATATGTAGGGATAAAAAAATTAAAAAACTTATTCTGTACCAT
TTTGAAAGGGAGGTAATTTT

Downstream 100 bases:

>100_bases
GGAAATTAAGACTTAAGAACCTTTAAAGAGTGAGAATTAAAACAAGAAATACTTTTAGTCAATAAATAGCTACAAAAATA
AAGAATACTAATATTTGTGC

Product: clpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 866; Mature: 866

Protein sequence:

>866_residues
MNIEKLTLKVQQAINDSQLVAVKYNHQQIDTIHLFSALISQDDGLIPNILGKMGVNIRDINAETNRVLDNMPKVLGEGAQ
SSSVYPTRRFEEVFVRAEQIAKDFKDSYISVEHVLLAIMDVDRNTVLPILEKFGVKKSEFLKALSAVRGSQRVDNQDPEG
TYEALVKYGRNLVEEAKKHKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAIVEGLAERIVRGDVPESLKNKI
IFSLDMGALIAGAKYRGEFEERLKAVLKEVQNSEGRIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELHCIGATTF
DEYRQYIEKDKALERRFQPVITDEPTVEDTVSILRGLKERFEIYHGIRIHDSAIVAAAKLSSRYITDRYLPDKAIDLIDE
ACAMIRTDIDSMPTEMDSMKRKIFQLEIEKEALSKEKDVASKERLEVLEKELSELKEKDKEMTAKYENEKSHITKIRDLK
EKLDDVRGQMEKAEREYDLNKVAELKYGLIPALQREIEEKEKLIKENSNGNAMLKEEVTEQEISEIISKWTGIPVTRLVE
TERQKLLQLGDQLETRVIGQGEAVKAVTNAVIRARAGLKDPRKPIGSFIFLGPTGVGKTELAKTLARTLFDTEENIIRID
MSEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKVIDFKNTI
IIMTSNLGSNYLLDNESKEGIDESIRTRVKEALKARFKPEFLNRVDDIIMFKPLTVEDIKRIIDIFLDDIRKRLKDKNIQ
LKITDSAKDIMAKEGYDPIYGARPLKRYIEDTIETEIAKQIIAGNIYEGTTVGVDLKGESIVIERI

Sequences:

>Translated_866_residues
MNIEKLTLKVQQAINDSQLVAVKYNHQQIDTIHLFSALISQDDGLIPNILGKMGVNIRDINAETNRVLDNMPKVLGEGAQ
SSSVYPTRRFEEVFVRAEQIAKDFKDSYISVEHVLLAIMDVDRNTVLPILEKFGVKKSEFLKALSAVRGSQRVDNQDPEG
TYEALVKYGRNLVEEAKKHKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAIVEGLAERIVRGDVPESLKNKI
IFSLDMGALIAGAKYRGEFEERLKAVLKEVQNSEGRIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELHCIGATTF
DEYRQYIEKDKALERRFQPVITDEPTVEDTVSILRGLKERFEIYHGIRIHDSAIVAAAKLSSRYITDRYLPDKAIDLIDE
ACAMIRTDIDSMPTEMDSMKRKIFQLEIEKEALSKEKDVASKERLEVLEKELSELKEKDKEMTAKYENEKSHITKIRDLK
EKLDDVRGQMEKAEREYDLNKVAELKYGLIPALQREIEEKEKLIKENSNGNAMLKEEVTEQEISEIISKWTGIPVTRLVE
TERQKLLQLGDQLETRVIGQGEAVKAVTNAVIRARAGLKDPRKPIGSFIFLGPTGVGKTELAKTLARTLFDTEENIIRID
MSEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKVIDFKNTI
IIMTSNLGSNYLLDNESKEGIDESIRTRVKEALKARFKPEFLNRVDDIIMFKPLTVEDIKRIIDIFLDDIRKRLKDKNIQ
LKITDSAKDIMAKEGYDPIYGARPLKRYIEDTIETEIAKQIIAGNIYEGTTVGVDLKGESIVIERI
>Mature_866_residues
MNIEKLTLKVQQAINDSQLVAVKYNHQQIDTIHLFSALISQDDGLIPNILGKMGVNIRDINAETNRVLDNMPKVLGEGAQ
SSSVYPTRRFEEVFVRAEQIAKDFKDSYISVEHVLLAIMDVDRNTVLPILEKFGVKKSEFLKALSAVRGSQRVDNQDPEG
TYEALVKYGRNLVEEAKKHKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAIVEGLAERIVRGDVPESLKNKI
IFSLDMGALIAGAKYRGEFEERLKAVLKEVQNSEGRIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELHCIGATTF
DEYRQYIEKDKALERRFQPVITDEPTVEDTVSILRGLKERFEIYHGIRIHDSAIVAAAKLSSRYITDRYLPDKAIDLIDE
ACAMIRTDIDSMPTEMDSMKRKIFQLEIEKEALSKEKDVASKERLEVLEKELSELKEKDKEMTAKYENEKSHITKIRDLK
EKLDDVRGQMEKAEREYDLNKVAELKYGLIPALQREIEEKEKLIKENSNGNAMLKEEVTEQEISEIISKWTGIPVTRLVE
TERQKLLQLGDQLETRVIGQGEAVKAVTNAVIRARAGLKDPRKPIGSFIFLGPTGVGKTELAKTLARTLFDTEENIIRID
MSEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKVIDFKNTI
IIMTSNLGSNYLLDNESKEGIDESIRTRVKEALKARFKPEFLNRVDDIIMFKPLTVEDIKRIIDIFLDDIRKRLKDKNIQ
LKITDSAKDIMAKEGYDPIYGARPLKRYIEDTIETEIAKQIIAGNIYEGTTVGVDLKGESIVIERI

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=300, Percent_Identity=38.3333333333333, Blast_Score=199, Evalue=1e-50,
Organism=Escherichia coli, GI1788943, Length=868, Percent_Identity=51.9585253456221, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=412, Percent_Identity=39.0776699029126, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=713, Percent_Identity=52.4544179523142, Blast_Score=749, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=854, Percent_Identity=43.3255269320843, Blast_Score=684, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 98255; Mature: 98255

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEKLTLKVQQAINDSQLVAVKYNHQQIDTIHLFSALISQDDGLIPNILGKMGVNIRDI
CCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEC
NAETNRVLDNMPKVLGEGAQSSSVYPTRRFEEVFVRAEQIAKDFKDSYISVEHVLLAIMD
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDRNTVLPILEKFGVKKSEFLKALSAVRGSQRVDNQDPEGTYEALVKYGRNLVEEAKKHK
CCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
LDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAIVEGLAERIVRGDVPESLKNKI
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCE
IFSLDMGALIAGAKYRGEFEERLKAVLKEVQNSEGRIILFIDEIHTIVGAGKTEGSMDAG
EEEECHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCCC
NLIKPMLARGELHCIGATTFDEYRQYIEKDKALERRFQPVITDEPTVEDTVSILRGLKER
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
FEIYHGIRIHDSAIVAAAKLSSRYITDRYLPDKAIDLIDEACAMIRTDIDSMPTEMDSMK
HHHHCCCEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RKIFQLEIEKEALSKEKDVASKERLEVLEKELSELKEKDKEMTAKYENEKSHITKIRDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EKLDDVRGQMEKAEREYDLNKVAELKYGLIPALQREIEEKEKLIKENSNGNAMLKEEVTE
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
QEISEIISKWTGIPVTRLVETERQKLLQLGDQLETRVIGQGEAVKAVTNAVIRARAGLKD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
PRKPIGSFIFLGPTGVGKTELAKTLARTLFDTEENIIRIDMSEYMEKYSVSRLIGAPPGY
CCHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCC
VGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKVIDFKNTI
CCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEE
IIMTSNLGSNYLLDNESKEGIDESIRTRVKEALKARFKPEFLNRVDDIIMFKPLTVEDIK
EEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHH
RIIDIFLDDIRKRLKDKNIQLKITDSAKDIMAKEGYDPIYGARPLKRYIEDTIETEIAKQ
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IIAGNIYEGTTVGVDLKGESIVIERI
HHHCCCCCCCEEEEEECCCEEEEECC
>Mature Secondary Structure
MNIEKLTLKVQQAINDSQLVAVKYNHQQIDTIHLFSALISQDDGLIPNILGKMGVNIRDI
CCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEC
NAETNRVLDNMPKVLGEGAQSSSVYPTRRFEEVFVRAEQIAKDFKDSYISVEHVLLAIMD
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDRNTVLPILEKFGVKKSEFLKALSAVRGSQRVDNQDPEGTYEALVKYGRNLVEEAKKHK
CCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
LDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAIVEGLAERIVRGDVPESLKNKI
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCE
IFSLDMGALIAGAKYRGEFEERLKAVLKEVQNSEGRIILFIDEIHTIVGAGKTEGSMDAG
EEEECHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCCC
NLIKPMLARGELHCIGATTFDEYRQYIEKDKALERRFQPVITDEPTVEDTVSILRGLKER
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
FEIYHGIRIHDSAIVAAAKLSSRYITDRYLPDKAIDLIDEACAMIRTDIDSMPTEMDSMK
HHHHCCCEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RKIFQLEIEKEALSKEKDVASKERLEVLEKELSELKEKDKEMTAKYENEKSHITKIRDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EKLDDVRGQMEKAEREYDLNKVAELKYGLIPALQREIEEKEKLIKENSNGNAMLKEEVTE
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
QEISEIISKWTGIPVTRLVETERQKLLQLGDQLETRVIGQGEAVKAVTNAVIRARAGLKD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
PRKPIGSFIFLGPTGVGKTELAKTLARTLFDTEENIIRIDMSEYMEKYSVSRLIGAPPGY
CCHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCC
VGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKVIDFKNTI
CCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEE
IIMTSNLGSNYLLDNESKEGIDESIRTRVKEALKARFKPEFLNRVDDIIMFKPLTVEDIK
EEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHH
RIIDIFLDDIRKRLKDKNIQLKITDSAKDIMAKEGYDPIYGARPLKRYIEDTIETEIAKQ
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IIAGNIYEGTTVGVDLKGESIVIERI
HHHCCCCCCCEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]