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The map label for this gene is topA [H]

Identifier: 153935195

GI number: 153935195

Start: 2413626

End: 2415728

Strand: Reverse

Name: topA [H]

Synonym: CLC_2285

Alternate gene names: 153935195

Gene position: 2415728-2413626 (Counterclockwise)

Preceding gene: 153936174

Following gene: 153937667

Centisome position: 64.24

GC content: 30.24

Gene sequence:

>2103_bases
ATGGGACAAAAAATGGGACAAAATTTAGTAATAGTAGAATCACCAGCAAAAGCTAAAACCATAAAGAAGTATTTAGGAAA
AAACTATGTAGTTGAGGCCTCAATGGGACATGTAAGAGATTTACCTAAAAGTCAATTAGGTGTTGATATAGAAAATAATT
ATGATCCTAAGTATATAACAATAAGAGGAAAAGGCGAACTTTTAGATAAACTTAGAAAAGAAGCTAAAAAAAGCTCTAAA
GTATATTTAGCAACGGACCCGGATAGAGAGGGAGAAGCTATTTCTTGGCATTTAGCTCATGTTTTAAAAATAGATGAGAG
TGAAGAATGCAGAATAGAATTTAACGAGATAACTAAAACTTCTATAAAGAAAGCAATCAAATCTCCGAGAAGAATAAATG
AAAATGTAGTAGACGCGCAGCAAGCAAGAAGGGTTTTGGATAGATTAGTTGGATATAAAATAAGCCCTATATTGTGGAGC
AAGATTAAATGGGGATTAAGTGCAGGTAGAGTTCAATCTGTGGCTTTAAGAATGATATGCGATAGAGAAAGAGAAATTGA
AGAATTTATACCAAAAGAATATTGGACTATAGAATGTGAGGTATATAAAGAAAAAAATAAAAAACCTTTCATAATAAAAT
TAACTACTAAATCAGGGAAAAAAATAGAGATAAATAATGAAGAGGAAGCTATTGAAGCAATACAAGACTTAAAAAAAGGT
GAATTTATAGTAAAAAAAATAAAGAAGACAAAGAAAAATAAAAATCCTTTAGCACCTTTTACTACGAGTACTCTTCAACA
GGATTCTTATAAAAAATTAAATTTTTCTACTAAAAAGACTATGTCTATAGCTCAACAACTATATGAAGGTATCGATATCA
AAGGTTTTGGTGCAGTAGGTCTTATAACCTACATGAGAACAGATTCTATAAGAATATCAGAAGAAGCTCAAAAATCAACC
CTAGAGCATATCAAAAGTAATTTTGGGGAAGAATATATACCAAAAACTCCAAGAGTTTTTAAAGGAAAGAAAAATATACA
GGATGCCCATGAAGCTATAAGACCAACCAATATTGAAATAACTCCTACACTTGCTAAGGAAAGTTTAACGCCTCAACAAT
ATAAATTATACGAATTAATATGGAAAAGATTTGTAGCAAGCCAAATGGCTTCTTGTATTTTAGATTCAACATCTATAAGT
ATAGAAAATGGAAAATATGGATTAAGAGCTAGTGGATCCTTAATAGCTTTTGATGGTTTTATGAAGGTGTATGAGTATTC
TACAGAGGAAGAAAAAGATAGTATAAAAATACCTGAGTTGGAAGATAATGAGATTTTGGGAGAGAAATCTATAAACAAAA
GCCAACATTTTACTCAACCACCAGCTAGGTATTCTGAAGCTTCTTTAGTAAAAACTTTAGAAGAAAATGGTATAGGAAGG
CCAAGTACTTATGCTCCTATTATATCTACTATATTAGATAGAAAATATGTAGAAAGAGAGAAAAAAACATTAAAACCTAC
AGAACTAGGAGATATAGTAAATAATATACTAAGTGAATATTTTAAGCAAATAGTAGATCTAGAATTTACAGCGGATATGG
AAAATAAGCTAGATTATATAGAAGAAGGAAAAAAATCTTGGAGAGAGGTAGTAAATGATTTCTTTCAGCCATTGATAGAA
TCCATAGAAATAGCAGAAAAAGAAATAGCAAAAATAACTATTGAAGATAAAGTAACAGATGTGGTATGTGAAAAATGTGG
AAGAAATATGGTTATAAAACATGGAAGATATGGAGACTTTTTAGCATGTCCGGGATATCCAGATTGTAAAAATACCAAAC
CTATAGTAGAAGAAATAGATGTTAAGTGTCCTAAATGTCGAGATGGAAAAGTCTTAATAAAGAAAAGTAGAAAAGGCAGA
AAGTTTTATGGATGCAGTAACTATCCAGACTGTGATTTTGTAAGTTGGTCAGAACCTGTGAAAGAAAAGTGTGAAGAATG
TGGAAGTTACATGGTAAAAAAACAAAGTAAAGCTAAAGGTGAATACTATGAATGTGCTAATTCAGAATGTAAGCATAGAA
AATATTTAAATAATAAAGAGTAA

Upstream 100 bases:

>100_bases
GTTTAATAAAAATAGTAGCACCATAAAGAGGTGTAATTCTAAATTAGTATATCAGGTAAGGGGGTGTGACTCTGGTTATC
AGTTTTACCAGAGAAATAGA

Downstream 100 bases:

>100_bases
TGTATCAATAATTAATTTATATAAATAATTATTATTTAATATATTTTAAATGTTGAAATAAACAAACCCTTATGTTATTA
TAGATAAGGACAGGCAACAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MGQKMGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGKGELLDKLRKEAKKSSK
VYLATDPDREGEAISWHLAHVLKIDESEECRIEFNEITKTSIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWS
KIKWGLSAGRVQSVALRMICDREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKG
EFIVKKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITYMRTDSIRISEEAQKST
LEHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSIS
IENGKYGLRASGSLIAFDGFMKVYEYSTEEEKDSIKIPELEDNEILGEKSINKSQHFTQPPARYSEASLVKTLEENGIGR
PSTYAPIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGKKSWREVVNDFFQPLIE
SIEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACPGYPDCKNTKPIVEEIDVKCPKCRDGKVLIKKSRKGR
KFYGCSNYPDCDFVSWSEPVKEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE

Sequences:

>Translated_700_residues
MGQKMGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGKGELLDKLRKEAKKSSK
VYLATDPDREGEAISWHLAHVLKIDESEECRIEFNEITKTSIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWS
KIKWGLSAGRVQSVALRMICDREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKG
EFIVKKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITYMRTDSIRISEEAQKST
LEHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSIS
IENGKYGLRASGSLIAFDGFMKVYEYSTEEEKDSIKIPELEDNEILGEKSINKSQHFTQPPARYSEASLVKTLEENGIGR
PSTYAPIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGKKSWREVVNDFFQPLIE
SIEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACPGYPDCKNTKPIVEEIDVKCPKCRDGKVLIKKSRKGR
KFYGCSNYPDCDFVSWSEPVKEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE
>Mature_699_residues
GQKMGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGKGELLDKLRKEAKKSSKV
YLATDPDREGEAISWHLAHVLKIDESEECRIEFNEITKTSIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSK
IKWGLSAGRVQSVALRMICDREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKGE
FIVKKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITYMRTDSIRISEEAQKSTL
EHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSISI
ENGKYGLRASGSLIAFDGFMKVYEYSTEEEKDSIKIPELEDNEILGEKSINKSQHFTQPPARYSEASLVKTLEENGIGRP
STYAPIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGKKSWREVVNDFFQPLIES
IEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACPGYPDCKNTKPIVEEIDVKCPKCRDGKVLIKKSRKGRK
FYGCSNYPDCDFVSWSEPVKEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=616, Percent_Identity=24.025974025974, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI4507635, Length=551, Percent_Identity=23.5934664246824, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787529, Length=765, Percent_Identity=39.6078431372549, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1788061, Length=643, Percent_Identity=23.4836702954899, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI48994931, Length=140, Percent_Identity=33.5714285714286, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=511, Percent_Identity=25.8317025440313, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=505, Percent_Identity=23.7623762376238, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=549, Percent_Identity=22.9508196721311, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585251, Length=636, Percent_Identity=24.685534591195, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640096, Length=650, Percent_Identity=22.3076923076923, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80332; Mature: 80201

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQKMGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYIT
CCCCCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCHHHCCCHHHCCCCCCCCCCCCEEE
IRGKGELLDKLRKEAKKSSKVYLATDPDREGEAISWHLAHVLKIDESEECRIEFNEITKT
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEHHHEECCCCCCCEEEHHHHHHH
SIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKIKWGLSAGRVQSVALRMIC
HHHHHHCCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
DREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKG
CCHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCC
EFIVKKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVG
HHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHH
LITYMRTDSIRISEEAQKSTLEHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEI
HHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCEEE
TPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENGKYGLRASGSLIAFDGF
EECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEHHH
MKVYEYSTEEEKDSIKIPELEDNEILGEKSINKSQHFTQPPARYSEASLVKTLEENGIGR
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PSTYAPIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYI
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHH
EEGKKSWREVVNDFFQPLIESIEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEECCCCCCE
LACPGYPDCKNTKPIVEEIDVKCPKCRDGKVLIKKSRKGRKFYGCSNYPDCDFVSWSEPV
EECCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCHHH
KEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCCCC
>Mature Secondary Structure 
GQKMGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYIT
CCCCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCHHHCCCHHHCCCCCCCCCCCCEEE
IRGKGELLDKLRKEAKKSSKVYLATDPDREGEAISWHLAHVLKIDESEECRIEFNEITKT
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEHHHEECCCCCCCEEEHHHHHHH
SIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKIKWGLSAGRVQSVALRMIC
HHHHHHCCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
DREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKG
CCHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCC
EFIVKKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVG
HHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHH
LITYMRTDSIRISEEAQKSTLEHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEI
HHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCEEE
TPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENGKYGLRASGSLIAFDGF
EECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEHHH
MKVYEYSTEEEKDSIKIPELEDNEILGEKSINKSQHFTQPPARYSEASLVKTLEENGIGR
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PSTYAPIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYI
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHH
EEGKKSWREVVNDFFQPLIESIEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEECCCCCCE
LACPGYPDCKNTKPIVEEIDVKCPKCRDGKVLIKKSRKGRKFYGCSNYPDCDFVSWSEPV
EECCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCHHH
KEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]