Click here to switch to the map view.

The map label for this gene is ydeM [C]

Identifier: 153935053

GI number: 153935053

Start: 650694

End: 652046

Strand: Direct

Name: ydeM [C]

Synonym: CLC_0636

Alternate gene names: 153935053

Gene position: 650694-652046 (Clockwise)

Preceding gene: 153936644

Following gene: 153935881

Centisome position: 17.3

GC content: 29.64

Gene sequence:

>1353_bases
ATGAAAAAATCTAAATACAATAAAATAATCAAATTAGAAGATGGAAAAACTATAGCTTTTAATAGTGTTACTTGTGCGTT
AGCAGAGGTAGATCAGGATTTCTTAGCTGTATTAGCAAACATAGAAAATATAGATATAGAAAATTTAGATGAAAAAAAGA
GAGAGTTAGTTGAAAACATGTCAAAGGGTAATTATATAGTTCATGATGATATGGATGAATTAAAGTTATTAAAATATAGA
AATTACAATGGGAAGTTTTCAAGTGGCGCATTAGGACTAGTTATTGCTCCTACACTAGCTTGTAATTTTGCTTGTCCTTA
TTGTTATGAAACTCCAAAAGCAGGCCTTATGGACAAAAAAGTTCAAGATAGTTTAATAGAAATGATAGAAGAAAATGCTA
AAAATAAAAATAATATAAGTATAACTTGGTATGGTGGAGAACCCTTATTAGCAAAGGAGATGATATTTGACTTTTCTCAA
AGGGCTATAGAAATATGTGAAAGAGAAAATGTTAAATATAGTGCATATATTGTAACCAATGGATACTTGATAGATGAAGA
AACTATAGAAAATATGAAAAAAGCTAAAATAACAGGGGCGCAAATTACAGTGGATGGCCCTCCAAGTATTCATAATAAAA
GAAGAATATTAAAGAATTCAGATGAGGAAACTTTTACTACAATAATGGCTAATGTAAAAAAACTTATAGAGGGTGGAATT
AAAAATATAGCTATTAGAATAAATGTAGATAAAACAAATGTTGATCATGTGGAGGAATTATTAGATATATTAGAAGAAAA
TGGATTGAAAGATGTTGTTGTTAATCTAGGACATGTTACTGCATATACAGATAGTTGTAACGGAATTGCAAATAATTGTT
TAAATACAAAAGAATATGCAAAAAGTGATACTAAATATCAGAAAGTACTTTTTGAAAGAGGATATAAGGTGGCAGGAGCA
TATCCTTTCTATCCAAGCGTAAAAGCAAATTATTGTTGTGCTGATAATGTTGGAGCTTATGTAATTGATTCTGAAGGATA
TATGTATAAATGTTGGAATGATGTTGGAAATGTTGATAGGGCGGTTGGAAATGTTACTACAATTAAAGACAAAGTAGATG
AAAAAATGTATGCAAGAAATATGGAATATATATTATGGTCTCCATTTGAATATGAAGAGTGTAAGGAGTGTAGCATATTA
CCAATATGCATGGGAGGATGTCCATATAAAGGAACAATGAATGAGAAGCCTGAATGTGAAAAATGGATATATAACCTTGA
AGAAACAATAATAGCAACATATAACCAAAAAAATGAGGCTGAATGTAGTCAGCAGGGATGTTGCTGTGAATAA

Upstream 100 bases:

>100_bases
ATAACTAAATAATATAGGAACAAATAACGCAATAATAAAATAAATTTAGATTATTATACATTAGATTATTAAAAATTTAA
CAAAGTAGGGGGCAAAATTT

Downstream 100 bases:

>100_bases
TCTTAATTTCCATATTAAAGATTTAAGAGTATTAAGAGATATTGAAAGCTATTTGGATTTTTATAATAGAGCAATGAATA
TAAAAAATGTATTTAATTTT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKKSKYNKIIKLEDGKTIAFNSVTCALAEVDQDFLAVLANIENIDIENLDEKKRELVENMSKGNYIVHDDMDELKLLKYR
NYNGKFSSGALGLVIAPTLACNFACPYCYETPKAGLMDKKVQDSLIEMIEENAKNKNNISITWYGGEPLLAKEMIFDFSQ
RAIEICERENVKYSAYIVTNGYLIDEETIENMKKAKITGAQITVDGPPSIHNKRRILKNSDEETFTTIMANVKKLIEGGI
KNIAIRINVDKTNVDHVEELLDILEENGLKDVVVNLGHVTAYTDSCNGIANNCLNTKEYAKSDTKYQKVLFERGYKVAGA
YPFYPSVKANYCCADNVGAYVIDSEGYMYKCWNDVGNVDRAVGNVTTIKDKVDEKMYARNMEYILWSPFEYEECKECSIL
PICMGGCPYKGTMNEKPECEKWIYNLEETIIATYNQKNEAECSQQGCCCE

Sequences:

>Translated_450_residues
MKKSKYNKIIKLEDGKTIAFNSVTCALAEVDQDFLAVLANIENIDIENLDEKKRELVENMSKGNYIVHDDMDELKLLKYR
NYNGKFSSGALGLVIAPTLACNFACPYCYETPKAGLMDKKVQDSLIEMIEENAKNKNNISITWYGGEPLLAKEMIFDFSQ
RAIEICERENVKYSAYIVTNGYLIDEETIENMKKAKITGAQITVDGPPSIHNKRRILKNSDEETFTTIMANVKKLIEGGI
KNIAIRINVDKTNVDHVEELLDILEENGLKDVVVNLGHVTAYTDSCNGIANNCLNTKEYAKSDTKYQKVLFERGYKVAGA
YPFYPSVKANYCCADNVGAYVIDSEGYMYKCWNDVGNVDRAVGNVTTIKDKVDEKMYARNMEYILWSPFEYEECKECSIL
PICMGGCPYKGTMNEKPECEKWIYNLEETIIATYNQKNEAECSQQGCCCE
>Mature_450_residues
MKKSKYNKIIKLEDGKTIAFNSVTCALAEVDQDFLAVLANIENIDIENLDEKKRELVENMSKGNYIVHDDMDELKLLKYR
NYNGKFSSGALGLVIAPTLACNFACPYCYETPKAGLMDKKVQDSLIEMIEENAKNKNNISITWYGGEPLLAKEMIFDFSQ
RAIEICERENVKYSAYIVTNGYLIDEETIENMKKAKITGAQITVDGPPSIHNKRRILKNSDEETFTTIMANVKKLIEGGI
KNIAIRINVDKTNVDHVEELLDILEENGLKDVVVNLGHVTAYTDSCNGIANNCLNTKEYAKSDTKYQKVLFERGYKVAGA
YPFYPSVKANYCCADNVGAYVIDSEGYMYKCWNDVGNVDRAVGNVTTIKDKVDEKMYARNMEYILWSPFEYEECKECSIL
PICMGGCPYKGTMNEKPECEKWIYNLEETIIATYNQKNEAECSQQGCCCE

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine ('Cys-51' in the arylsulfatase CPF_0221) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical c

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=343, Percent_Identity=23.0320699708455, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 50972; Mature: 50972

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSKYNKIIKLEDGKTIAFNSVTCALAEVDQDFLAVLANIENIDIENLDEKKRELVENM
CCCCCCCCEEEECCCCEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SKGNYIVHDDMDELKLLKYRNYNGKFSSGALGLVIAPTLACNFACPYCYETPKAGLMDKK
CCCCEEEECCHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCHHH
VQDSLIEMIEENAKNKNNISITWYGGEPLLAKEMIFDFSQRAIEICERENVKYSAYIVTN
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
GYLIDEETIENMKKAKITGAQITVDGPPSIHNKRRILKNSDEETFTTIMANVKKLIEGGI
CEEECHHHHHHHHHHEECCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
KNIAIRINVDKTNVDHVEELLDILEENGLKDVVVNLGHVTAYTDSCNGIANNCLNTKEYA
CEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHCCCCHHHH
KSDTKYQKVLFERGYKVAGAYPFYPSVKANYCCADNVGAYVIDSEGYMYKCWNDVGNVDR
HCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCEEEECCCCCEEEEHHHHCHHHH
AVGNVTTIKDKVDEKMYARNMEYILWSPFEYEECKECSILPICMGGCPYKGTMNEKPECE
HHCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCHH
KWIYNLEETIIATYNQKNEAECSQQGCCCE
HHHHHHHHHEEEECCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKKSKYNKIIKLEDGKTIAFNSVTCALAEVDQDFLAVLANIENIDIENLDEKKRELVENM
CCCCCCCCEEEECCCCEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SKGNYIVHDDMDELKLLKYRNYNGKFSSGALGLVIAPTLACNFACPYCYETPKAGLMDKK
CCCCEEEECCHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCHHH
VQDSLIEMIEENAKNKNNISITWYGGEPLLAKEMIFDFSQRAIEICERENVKYSAYIVTN
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
GYLIDEETIENMKKAKITGAQITVDGPPSIHNKRRILKNSDEETFTTIMANVKKLIEGGI
CEEECHHHHHHHHHHEECCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
KNIAIRINVDKTNVDHVEELLDILEENGLKDVVVNLGHVTAYTDSCNGIANNCLNTKEYA
CEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHCCCCHHHH
KSDTKYQKVLFERGYKVAGAYPFYPSVKANYCCADNVGAYVIDSEGYMYKCWNDVGNVDR
HCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCEEEECCCCCEEEEHHHHCHHHH
AVGNVTTIKDKVDEKMYARNMEYILWSPFEYEECKECSILPICMGGCPYKGTMNEKPECE
HHCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCHH
KWIYNLEETIIATYNQKNEAECSQQGCCCE
HHHHHHHHHEEEECCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA