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The map label for this gene is ptsG [H]

Identifier: 153934903

GI number: 153934903

Start: 323839

End: 324348

Strand: Direct

Name: ptsG [H]

Synonym: CLC_0338

Alternate gene names: 153934903

Gene position: 323839-324348 (Clockwise)

Preceding gene: 153937811

Following gene: 153935850

Centisome position: 8.61

GC content: 30.2

Gene sequence:

>510_bases
ATGTTTAAAAAAATTAAATCTCTTTTATCGAATGATAAATCAGACGTTCAACAAGAAAATTTAAATGAAGTATTTGTAAG
CCCTATTTCTGGAGAAATAATAAGTCTAGATGATGTTCCAGATGAGGTATTTTCACAAAGAATGATGGGAGATGGGTTTG
CCATACAACCTGAAAATGGAGATGTATTTTCACCAGTGGATGGAACAATTACAGCAGTTTTCCCTACCAAACATGCTATT
TCTATAAAAAGCGAATCTGGAGTTGAGATTTTAATCCATTTTGGATTAGATACAGTTAACTTAAATGGAGAAGGTTTTCA
GGTTTATGTTGAGGAAGGAAATCAAGTAAAAGCAGGAGATATGTTACTAAAGGTTAATATTGAAGAAATAAAGGATAAAG
TTCCTTCACTTATTGTTCCTATAATATTTATGGATCTTAATGGAAAGAATTTTAGTTATAATACTGGTAAGGTTACAGCT
AAAGAGCCAAATATAATAACATTAAAGTAA

Upstream 100 bases:

>100_bases
TACAGTTAAATAAATTATAGATTATATATGTACTATGTATAAAACAAGTAAAAAAGTTTTGTGTAGTAGTGTACACGAGA
TATTATTAGGAGGAATTAAA

Downstream 100 bases:

>100_bases
TCTGCATATATACATATTTTAAAATTCTAATTATGATAATTATATATTTTAAGTTTATTAGTTTATAAAAATTTCTAACA
CATGTTCCTTTGTTATATTT

Product: PTS system, glucose/glucoside family, IIA component

Products: NA

Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MFKKIKSLLSNDKSDVQQENLNEVFVSPISGEIISLDDVPDEVFSQRMMGDGFAIQPENGDVFSPVDGTITAVFPTKHAI
SIKSESGVEILIHFGLDTVNLNGEGFQVYVEEGNQVKAGDMLLKVNIEEIKDKVPSLIVPIIFMDLNGKNFSYNTGKVTA
KEPNIITLK

Sequences:

>Translated_169_residues
MFKKIKSLLSNDKSDVQQENLNEVFVSPISGEIISLDDVPDEVFSQRMMGDGFAIQPENGDVFSPVDGTITAVFPTKHAI
SIKSESGVEILIHFGLDTVNLNGEGFQVYVEEGNQVKAGDMLLKVNIEEIKDKVPSLIVPIIFMDLNGKNFSYNTGKVTA
KEPNIITLK
>Mature_169_residues
MFKKIKSLLSNDKSDVQQENLNEVFVSPISGEIISLDDVPDEVFSQRMMGDGFAIQPENGDVFSPVDGTITAVFPTKHAI
SIKSESGVEILIHFGLDTVNLNGEGFQVYVEEGNQVKAGDMLLKVNIEEIKDKVPSLIVPIIFMDLNGKNFSYNTGKVTA
KEPNIITLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=170, Percent_Identity=41.7647058823529, Blast_Score=143, Evalue=5e-36,
Organism=Escherichia coli, GI1786894, Length=123, Percent_Identity=42.2764227642276, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1790159, Length=141, Percent_Identity=41.1347517730496, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR011535 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18575; Mature: 18575

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKIKSLLSNDKSDVQQENLNEVFVSPISGEIISLDDVPDEVFSQRMMGDGFAIQPENG
CHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCC
DVFSPVDGTITAVFPTKHAISIKSESGVEILIHFGLDTVNLNGEGFQVYVEEGNQVKAGD
CEECCCCCEEEEEECCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEECCCEEECCC
MLLKVNIEEIKDKVPSLIVPIIFMDLNGKNFSYNTGKVTAKEPNIITLK
EEEEEEHHHHHHHCCCEEEEEEEEECCCCCEECCCCEEEECCCCEEEEC
>Mature Secondary Structure
MFKKIKSLLSNDKSDVQQENLNEVFVSPISGEIISLDDVPDEVFSQRMMGDGFAIQPENG
CHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCC
DVFSPVDGTITAVFPTKHAISIKSESGVEILIHFGLDTVNLNGEGFQVYVEEGNQVKAGD
CEECCCCCEEEEEECCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEECCCEEECCC
MLLKVNIEEIKDKVPSLIVPIIFMDLNGKNFSYNTGKVTAKEPNIITLK
EEEEEEHHHHHHHCCCEEEEEEEEECCCCCEECCCCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7670643 [H]