| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
Click here to switch to the map view.
The map label for this gene is pcrA [H]
Identifier: 153934674
GI number: 153934674
Start: 757560
End: 760214
Strand: Direct
Name: pcrA [H]
Synonym: CLC_0737
Alternate gene names: 153934674
Gene position: 757560-760214 (Clockwise)
Preceding gene: 153937631
Following gene: 153936446
Centisome position: 20.14
GC content: 28.44
Gene sequence:
>2655_bases ATGCATTTAGATGTTATTAGTGGACTTAATGACGCTCAAAGAGAAGCTGTTACCACAACAGAAGGCTATATACGTGTAAT TGCTGGAGCTGGCAGTGGGAAGACGAAAGCATTGACAAGTAGATATATTTATCTAGTACATGAATTAGGGATTTCGACAG CTAATATTTTATGTGCAACTTTTACCAATAAAGCAGCAAGAGAGATGAAAAAACGTATAAGGACGATGATAGGTGATAAT GATACAGGATTAATTGGTACTTTTCATGGATTTTGCAGACGTTTGTTAAAAGAAGATATATATACAATTAATTATCCTGA CAATTTTATAGTTATGGATAATGAGGATATGGATTTAGTATTAAGAATTGTGTATGAAGATGCAAATATATCTTCAAAGC AATTTACATTTTCTAAAGCCAAAGATATGATTAGTAAGAGGAAAAATAAGGATTTTAAATATATATCATATATAATAGAG ACAGATAATAATGAATTGAAGAAAAAATTTTTAACATCAAAGAATGTTGAAGATAGAATCTTCTATGGTTATTTATATCA ACAAAAGAAAAATTATAATTTAGATTTTAATGATTTAATAATATTTACATTGCATATATTAGAAACGTTTCCTAAGGTGA GAAAAAAATGGCAACAAAAACTTGAATATATAATGGTGGATGAATTTCAAGATGTAAATATAAAGCAATATAGGTTAGTT AGTATATTGAGTGAATATCATAAAAATTTATTTGTTGTTGGAGATCCAGACCAAACAGTTTATTCTTGGAGAGGTGCTAA TATAAACTTAATATTAAATTTTGATAAAGAGTTCAAGGGAACAAAAACAATAATGATGAATCAAAACTATAGATCATCAT CAAATATTATAAAAGCTTCTAATTCATTAATTTCTAAAAATAAACAAAGAATTGATAAGGAATTAATCCCAATAAAGCAT GGAAATATTCCAGTGGTATACAAACATTCAGAAAACATATATAAGGAAGCTAATTGGATTATAAATCAAATTGAGCAAAT AATTAAAGGTGGAAATAGCTATAAGAATATAGCTATTCTTTATAGAGCTCATTATGTATCGAGAAGCATAGAAGAAGCTT TATTAAAAAATAAAATTCCATATACATTGTATAGTGGTGTTGAATTTTATCAGAGAAAAGAAATTAAAGACATTTTAAGC TATCTAAGGATGGTCGTTTTTGAAGATGACTTATCATTCATTCGTATTATAAATGAACCAAAAAGAAATTTTGGTAAAAA GAGAATGGAGATCATTAGGAAGTATGCTGAAGATAATAATTGCTCATTATATAGTTCATTGAAGATAAATCTTGAAGATA AGCTAATTGCAAAAAGTAAGGCAGATAAATTTGTAGATCTTATTGAAAGATATAAAAAAATATATAAAGAAATGAGAATT ACAGATTTACTTATGGAATTATTAAATAATAGTGGTTATGAAGCTATGTTAAGAGAAAGTGGGGAAGACGAACGTCTTGA AAATTTAGCAGAACTAAAGAATTCAATATCAGATTATGAAAATAGTGCTGGGGAAGATACTACACTAGAAGATTATTTGC AAGAAATATCTTTATTTACTAATTCAGATTTGAAGGAAAATAAAGATACAATTAATATGATGACATTACATACAGCAAAG GGATTAGAATTTCCCTATGTTTTTATATGCGAATTTAATGAAGGCATATTTCCTAGCGCAAGAACTAATACAAAAGAAAA AATGGAAGAAGAACGTAGATTAGCATATGTAGGATATACGAGAGCTAAAAATGCATTGTTTTTAAGTGATGCAGAAGGGG TTAATTATGACGGCTCATATCGCTTCCCATCAAGATTTATTTTTAATACAGGAAAAACGTACTTAAATTATTTAGTAGAG CTAAAAGAAAGTCTTATAGATGATGCAAATGAGTTTATTAGTGATTCCGAAAATTCTATGAATAAGAGTAATAATATTAA ATTTAAAATTGGAGAAAGAATAATACATAAATTGCTTGGGCTAGGCGTTATTATTGGCATAGATAATGATAATTCATCTT ATATGATTAAATTTGACAATGCAGAAACATTTAGAAATATTTCATTTTCTACACAATTAGACAAAGCTGATACCAATAAT TTAGATATTATTAAATCAAATGAACGGAAAGCAATAGATCATTTGGAAGCGGAACGATTAGAGGCAGAGAGAAAAGAGCA AGAAAGATTGGAAGCAGAACGATTAGAGGCAGAGAGAAAAGAGCAAGAAAGATTGGAAGCAGAACGATTAGAGGCAGAGA GAAAAGAGCAAGAAAGATTGGAAGCAGAACGATTAGAGGCAGAGAGAAAAGAGCAAGAAAGATTGGAAGCAGAACGATTA GAGGCAGAGAGAAAAGAGCAAGAAAGATTGGAAGCGGAACGATTAGAGGCAGAGAGAAAAGAGCAAGAGAGACTAGAAGC AGAACAAATAGAAGTTAGTACAATGATTTTAAAAATAAAAGAAGAAATATCAGAGTTAGAAGAAATAGTGTCAAAAAATA AATATAAGCTATTCGGAAAAGGTGCTATATTAAAAAAGGAGGCAAAAAAGAGAATACAAATTTTATATTTAAAGATAGAT GAAATAAGCAACTAA
Upstream 100 bases:
>100_bases TAAAGTGGCTGAAATTAAAAATAATGCTGAAATAATTATTTAATTGATAAAAATAAGAATACTTAAAAAATAGGATTATA AACTTAGGGGGAGGGATTTC
Downstream 100 bases:
>100_bases TTTAAATTATGGAAAATTATCAGTTAATATAATAGTTGAATAATAATAGAAGATGAAATTGTTATTATACTTATTACTAA ATCAAAAAGACTGGGTATAT
Product: putative ATP-dependent DNA helicase PcrA
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 884; Mature: 884
Protein sequence:
>884_residues MHLDVISGLNDAQREAVTTTEGYIRVIAGAGSGKTKALTSRYIYLVHELGISTANILCATFTNKAAREMKKRIRTMIGDN DTGLIGTFHGFCRRLLKEDIYTINYPDNFIVMDNEDMDLVLRIVYEDANISSKQFTFSKAKDMISKRKNKDFKYISYIIE TDNNELKKKFLTSKNVEDRIFYGYLYQQKKNYNLDFNDLIIFTLHILETFPKVRKKWQQKLEYIMVDEFQDVNIKQYRLV SILSEYHKNLFVVGDPDQTVYSWRGANINLILNFDKEFKGTKTIMMNQNYRSSSNIIKASNSLISKNKQRIDKELIPIKH GNIPVVYKHSENIYKEANWIINQIEQIIKGGNSYKNIAILYRAHYVSRSIEEALLKNKIPYTLYSGVEFYQRKEIKDILS YLRMVVFEDDLSFIRIINEPKRNFGKKRMEIIRKYAEDNNCSLYSSLKINLEDKLIAKSKADKFVDLIERYKKIYKEMRI TDLLMELLNNSGYEAMLRESGEDERLENLAELKNSISDYENSAGEDTTLEDYLQEISLFTNSDLKENKDTINMMTLHTAK GLEFPYVFICEFNEGIFPSARTNTKEKMEEERRLAYVGYTRAKNALFLSDAEGVNYDGSYRFPSRFIFNTGKTYLNYLVE LKESLIDDANEFISDSENSMNKSNNIKFKIGERIIHKLLGLGVIIGIDNDNSSYMIKFDNAETFRNISFSTQLDKADTNN LDIIKSNERKAIDHLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAERL EAERKEQERLEAERLEAERKEQERLEAEQIEVSTMILKIKEEISELEEIVSKNKYKLFGKGAILKKEAKKRIQILYLKID EISN
Sequences:
>Translated_884_residues MHLDVISGLNDAQREAVTTTEGYIRVIAGAGSGKTKALTSRYIYLVHELGISTANILCATFTNKAAREMKKRIRTMIGDN DTGLIGTFHGFCRRLLKEDIYTINYPDNFIVMDNEDMDLVLRIVYEDANISSKQFTFSKAKDMISKRKNKDFKYISYIIE TDNNELKKKFLTSKNVEDRIFYGYLYQQKKNYNLDFNDLIIFTLHILETFPKVRKKWQQKLEYIMVDEFQDVNIKQYRLV SILSEYHKNLFVVGDPDQTVYSWRGANINLILNFDKEFKGTKTIMMNQNYRSSSNIIKASNSLISKNKQRIDKELIPIKH GNIPVVYKHSENIYKEANWIINQIEQIIKGGNSYKNIAILYRAHYVSRSIEEALLKNKIPYTLYSGVEFYQRKEIKDILS YLRMVVFEDDLSFIRIINEPKRNFGKKRMEIIRKYAEDNNCSLYSSLKINLEDKLIAKSKADKFVDLIERYKKIYKEMRI TDLLMELLNNSGYEAMLRESGEDERLENLAELKNSISDYENSAGEDTTLEDYLQEISLFTNSDLKENKDTINMMTLHTAK GLEFPYVFICEFNEGIFPSARTNTKEKMEEERRLAYVGYTRAKNALFLSDAEGVNYDGSYRFPSRFIFNTGKTYLNYLVE LKESLIDDANEFISDSENSMNKSNNIKFKIGERIIHKLLGLGVIIGIDNDNSSYMIKFDNAETFRNISFSTQLDKADTNN LDIIKSNERKAIDHLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAERL EAERKEQERLEAERLEAERKEQERLEAEQIEVSTMILKIKEEISELEEIVSKNKYKLFGKGAILKKEAKKRIQILYLKID EISN >Mature_884_residues MHLDVISGLNDAQREAVTTTEGYIRVIAGAGSGKTKALTSRYIYLVHELGISTANILCATFTNKAAREMKKRIRTMIGDN DTGLIGTFHGFCRRLLKEDIYTINYPDNFIVMDNEDMDLVLRIVYEDANISSKQFTFSKAKDMISKRKNKDFKYISYIIE TDNNELKKKFLTSKNVEDRIFYGYLYQQKKNYNLDFNDLIIFTLHILETFPKVRKKWQQKLEYIMVDEFQDVNIKQYRLV SILSEYHKNLFVVGDPDQTVYSWRGANINLILNFDKEFKGTKTIMMNQNYRSSSNIIKASNSLISKNKQRIDKELIPIKH GNIPVVYKHSENIYKEANWIINQIEQIIKGGNSYKNIAILYRAHYVSRSIEEALLKNKIPYTLYSGVEFYQRKEIKDILS YLRMVVFEDDLSFIRIINEPKRNFGKKRMEIIRKYAEDNNCSLYSSLKINLEDKLIAKSKADKFVDLIERYKKIYKEMRI TDLLMELLNNSGYEAMLRESGEDERLENLAELKNSISDYENSAGEDTTLEDYLQEISLFTNSDLKENKDTINMMTLHTAK GLEFPYVFICEFNEGIFPSARTNTKEKMEEERRLAYVGYTRAKNALFLSDAEGVNYDGSYRFPSRFIFNTGKTYLNYLVE LKESLIDDANEFISDSENSMNKSNNIKFKIGERIIHKLLGLGVIIGIDNDNSSYMIKFDNAETFRNISFSTQLDKADTNN LDIIKSNERKAIDHLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAERL EAERKEQERLEAERLEAERKEQERLEAEQIEVSTMILKIKEEISELEEIVSKNKYKLFGKGAILKKEAKKRIQILYLKID EISN
Specific function: Essential helicase [H]
COG id: COG0210
COG function: function code L; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI2367296, Length=719, Percent_Identity=32.9624478442281, Blast_Score=384, Evalue=1e-107, Organism=Escherichia coli, GI48994965, Length=646, Percent_Identity=33.7461300309598, Blast_Score=308, Evalue=1e-84, Organism=Saccharomyces cerevisiae, GI6322369, Length=755, Percent_Identity=28.0794701986755, Blast_Score=202, Evalue=1e-52, Organism=Saccharomyces cerevisiae, GI6324477, Length=483, Percent_Identity=24.6376811594203, Blast_Score=82, Evalue=3e-16,
Paralogues:
None
Copy number: 3000 [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005751 - InterPro: IPR013986 - InterPro: IPR014017 - InterPro: IPR000212 - InterPro: IPR014016 [H]
Pfam domain/function: PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 103731; Mature: 103731
Theoretical pI: Translated: 6.12; Mature: 6.12
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MHLDVISGLNDAQREAVTTTEGYIRVIAGAGSGKTKALTSRYIYLVHELGISTANILCAT CCCCHHCCCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHEEEH FTNKAAREMKKRIRTMIGDNDTGLIGTFHGFCRRLLKEDIYTINYPDNFIVMDNEDMDLV HHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEE LRIVYEDANISSKQFTFSKAKDMISKRKNKDFKYISYIIETDNNELKKKFLTSKNVEDRI EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCCHHE FYGYLYQQKKNYNLDFNDLIIFTLHILETFPKVRKKWQQKLEYIMVDEFQDVNIKQYRLV EEEEHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH SILSEYHKNLFVVGDPDQTVYSWRGANINLILNFDKEFKGTKTIMMNQNYRSSSNIIKAS HHHHHHCCCEEEEECCCCCEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHH NSLISKNKQRIDKELIPIKHGNIPVVYKHSENIYKEANWIINQIEQIIKGGNSYKNIAIL HHHHHHHHHHHHHHCCCEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE YRAHYVSRSIEEALLKNKIPYTLYSGVEFYQRKEIKDILSYLRMVVFEDDLSFIRIINEP EHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCH KRNFGKKRMEIIRKYAEDNNCSLYSSLKINLEDKLIAKSKADKFVDLIERYKKIYKEMRI HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH TDLLMELLNNSGYEAMLRESGEDERLENLAELKNSISDYENSAGEDTTLEDYLQEISLFT HHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC NSDLKENKDTINMMTLHTAKGLEFPYVFICEFNEGIFPSARTNTKEKMEEERRLAYVGYT CCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHEEEEEEH RAKNALFLSDAEGVNYDGSYRFPSRFIFNTGKTYLNYLVELKESLIDDANEFISDSENSM HCCCEEEEECCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC NKSNNIKFKIGERIIHKLLGLGVIIGIDNDNSSYMIKFDNAETFRNISFSTQLDKADTNN CCCCCEEEEHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCHHHHCCCCCCCCCCCCCCC LDIIKSNERKAIDHLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERL EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH EAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAEQIEVSTMILKIK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH EEISELEEIVSKNKYKLFGKGAILKKEAKKRIQILYLKIDEISN HHHHHHHHHHCCCCEEEEECCCHHHHHHHCCEEEEEEEECCCCC >Mature Secondary Structure MHLDVISGLNDAQREAVTTTEGYIRVIAGAGSGKTKALTSRYIYLVHELGISTANILCAT CCCCHHCCCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHEEEH FTNKAAREMKKRIRTMIGDNDTGLIGTFHGFCRRLLKEDIYTINYPDNFIVMDNEDMDLV HHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEE LRIVYEDANISSKQFTFSKAKDMISKRKNKDFKYISYIIETDNNELKKKFLTSKNVEDRI EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCCHHE FYGYLYQQKKNYNLDFNDLIIFTLHILETFPKVRKKWQQKLEYIMVDEFQDVNIKQYRLV EEEEHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH SILSEYHKNLFVVGDPDQTVYSWRGANINLILNFDKEFKGTKTIMMNQNYRSSSNIIKAS HHHHHHCCCEEEEECCCCCEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHH NSLISKNKQRIDKELIPIKHGNIPVVYKHSENIYKEANWIINQIEQIIKGGNSYKNIAIL HHHHHHHHHHHHHHCCCEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE YRAHYVSRSIEEALLKNKIPYTLYSGVEFYQRKEIKDILSYLRMVVFEDDLSFIRIINEP EHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCH KRNFGKKRMEIIRKYAEDNNCSLYSSLKINLEDKLIAKSKADKFVDLIERYKKIYKEMRI HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH TDLLMELLNNSGYEAMLRESGEDERLENLAELKNSISDYENSAGEDTTLEDYLQEISLFT HHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC NSDLKENKDTINMMTLHTAKGLEFPYVFICEFNEGIFPSARTNTKEKMEEERRLAYVGYT CCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHEEEEEEH RAKNALFLSDAEGVNYDGSYRFPSRFIFNTGKTYLNYLVELKESLIDDANEFISDSENSM HCCCEEEEECCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC NKSNNIKFKIGERIIHKLLGLGVIIGIDNDNSSYMIKFDNAETFRNISFSTQLDKADTNN CCCCCEEEEHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCHHHHCCCCCCCCCCCCCCC LDIIKSNERKAIDHLEAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERL EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH EAERLEAERKEQERLEAERLEAERKEQERLEAERLEAERKEQERLEAEQIEVSTMILKIK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH EEISELEEIVSKNKYKLFGKGAILKKEAKKRIQILYLKIDEISN HHHHHHHHHHCCCCEEEEECCCHHHHHHHCCEEEEEEEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA