| Definition | Clostridium botulinum A str. ATCC 19397, complete genome. |
|---|---|
| Accession | NC_009697 |
| Length | 3,863,450 |
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The map label for this gene is rne [H]
Identifier: 153932956
GI number: 153932956
Start: 3155246
End: 3156685
Strand: Reverse
Name: rne [H]
Synonym: CLB_3015
Alternate gene names: 153932956
Gene position: 3156685-3155246 (Counterclockwise)
Preceding gene: 153931732
Following gene: 153934256
Centisome position: 81.71
GC content: 23.96
Gene sequence:
>1440_bases GTGAAAGAAATATTTATAGAAAGGCAAAGAGAAAATGTAAGGATAGTTTTAAGAGAAAATAATCACATAAAAGAATTATT TATAGAAGAAGATAATAAATCTCCTCAGGTTGGAGAGATTTACGTAGGTATGGTTAAAAATATAATTCCTGCTATAAAAA GCGCTTTTGTAGATATAGGTTGGAATAAGAATGCATATCTTTATTTAGATAAAAAGTTCAATAATACCCATATAAAAAAA GGAGATTATATTTTAGTAGAAGTAGTTAAAGAGGACTTGAATAAAAAAGGTCCTAAAGTAACTAATGCTATAACTATACC GGGCAGATATACAGTTCTTCAAATTTTAAACAATGAAATTACATTTTCTCATAAAATAAAAGATGAAAAAATAAGAACAG AAATACAAAATAATATAGTAAAGCCTAAAGATGTAGGAATTTTAATAAGAACGGAAGCTATAAATACAAGTATAGAAAAT ATAAATAATGAGATAAGAAGATTATATAAAATATATAGTTCCCTTATAGAAAAATCTAAATATAAAATGAATACAGGAGT TCTATTTCAAAACGGAGGAATAGTAGGCAAAATACTAAGAGATAAATTAAACGATAATATATCAAAAATTTATTTAAATT GTAGAGAAGACTATGTTTATGTAAGTGAGTTTCTAAAAGAATACGGAGAAAACAATATAAAACTAGATATTTATGAAGGA GAAAGAAATCTTTTAGATTATTATGCTATAGAAAAAGAAATACTTTCTCTTAGAAATAAAAAAGTTTATTTAAATTGTGG AGGCTATATTATAATAGATAAAACTGAAGCAATGTATGTAGTGGACGTTAACTCTGGTAAAAATGTGAAGGGGAATTCCA TGGAGAAAACTATTTTTACTACTAATATGGAGGCTGCGGATGAAATTTGTAATCAAATTATACTTAGAAATTTAAATGGG ATTATTGTAATAGATTTTATAGATATGGATAATGAAAATCTTAAAGAAAAAGTCTTAGATAAATTAAAGCAGGGACTAAA AAGAGATAAAAATAAATCTGTTGTTTATCCCTTTACTGAATTAAATTTGGTTCAAATAGCTAGAAAAAGACAGGGCAAAA CAATATATGATTATATAGAACAGCCTTGCAAATGTTGTAGTGGAAAAGGGTCTATAGTTAGTTTTACTTATATGAAGTTA TTAATTAGAAATGAAATAATAAATATTTTGAATATGAGAGAGATAAAGGATATATATATAGAACTAAATAAAAGATATAA AGATTATATAGATAAAAATAGAATACAATTCATATCAGAAATTGAAGCTTTAGAAAAAAATATTTATATAAATTATATAG ATGCTGAAAATCAGTATAAGGTAGAACCATTAATATTTAAAAATCAAATAGAAGATGTAAAAGACCTTAAGATTTATTAA
Upstream 100 bases:
>100_bases TCCAGGGGCGTAGTCGCTCTTTGCTCCCACTGGGAGTATTAGAGCGAGTAGTCATCGGATAAATTATTTTCTTTATTTAA AGAAGGGAAGTGCCTTTAGA
Downstream 100 bases:
>100_bases TAAAATTTCTTTACAAAGTAAGGCCGCTATGGTAAAATGGCTTTTGTAGACCGCTCAGAAAAGGTGTTAAACATGATTTG CATGTACCTTAATTGGCGAG
Product: ribonuclease
Products: NA
Alternate protein names: RNase E [H]
Number of amino acids: Translated: 479; Mature: 479
Protein sequence:
>479_residues MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIGWNKNAYLYLDKKFNNTHIKK GDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEITFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIEN INNEIRRLYKIYSSLIEKSKYKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFTTNMEAADEICNQIILRNLNG IIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTELNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKL LIRNEIINILNMREIKDIYIELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY
Sequences:
>Translated_479_residues MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIGWNKNAYLYLDKKFNNTHIKK GDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEITFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIEN INNEIRRLYKIYSSLIEKSKYKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFTTNMEAADEICNQIILRNLNG IIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTELNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKL LIRNEIINILNMREIKDIYIELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY >Mature_479_residues MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIGWNKNAYLYLDKKFNNTHIKK GDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEITFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIEN INNEIRRLYKIYSSLIEKSKYKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFTTNMEAADEICNQIILRNLNG IIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTELNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKL LIRNEIINILNMREIKDIYIELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY
Specific function: Involved In The Processing Of The 5'End Of 16s Rrna. Could Be Involved In Chromosome Segregation And Cell Division. It May Be One Of The Components Of The Cytoplasmic Axial Filaments Bundles Or Merely Regulate The Formation Of This Structure. [C]
COG id: COG1530
COG function: function code J; Ribonucleases G and E
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 S1 motif domain [H]
Homologues:
Organism=Escherichia coli, GI87082235, Length=492, Percent_Identity=28.8617886178862, Blast_Score=214, Evalue=8e-57, Organism=Escherichia coli, GI1787325, Length=429, Percent_Identity=27.2727272727273, Blast_Score=191, Evalue=1e-49,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR003029 - InterPro: IPR019307 - InterPro: IPR022967 - InterPro: IPR004659 [H]
Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]
EC number: =3.1.26.12 [H]
Molecular weight: Translated: 56145; Mature: 56145
Theoretical pI: Translated: 8.97; Mature: 8.97
Prosite motif: PS50126 S1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIG CCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECC WNKNAYLYLDKKFNNTHIKKGDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEI CCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCE TFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIENINNEIRRLYKIYSSLIEKSK EEEECCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH YKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG CEECCCEEEECCCEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEC ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFT CCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEE TNMEAADEICNQIILRNLNGIIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTE CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC LNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKLLIRNEIINILNMREIKDIYI CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH ELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCEEEHHHHCCCHHCCCC >Mature Secondary Structure MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIG CCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECC WNKNAYLYLDKKFNNTHIKKGDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEI CCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCE TFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIENINNEIRRLYKIYSSLIEKSK EEEECCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH YKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG CEECCCEEEECCCEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEC ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFT CCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEE TNMEAADEICNQIILRNLNGIIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTE CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC LNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKLLIRNEIINILNMREIKDIYI CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH ELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCEEEHHHHCCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8905231 [H]