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The map label for this gene is 153932223

Identifier: 153932223

GI number: 153932223

Start: 3488121

End: 3489614

Strand: Reverse

Name: 153932223

Synonym: CLB_3358

Alternate gene names: NA

Gene position: 3489614-3488121 (Counterclockwise)

Preceding gene: 153930996

Following gene: 153934300

Centisome position: 90.32

GC content: 33.6

Gene sequence:

>1494_bases
ATGTTAAAGTTTGAAACTGAACTAAAGAAAATAAAATATTTAGTTTTAAAAGAAGTTGCGCAGTTAGCTATTGAAGACAA
TATGGATAAGTTAGAAATGTTGAAAATACCGTATAAAATATTAAATGAAAAACAAGCGGAATACAGATGTTGTGTTTATA
GAGAAAGAACGGTGGTATATGAAAGAGCTCAGTTAGCAGCTGGCTTTAAGCCAGATGATAACTTAACAAATGAATTAGAG
AGCATAAGTGATAATGACCAGATAATATATGTAATAGCTTCTGCCTGTGATCAATGTCCTTTAAATAGATTTAGAGTAAC
AGAAGCTTGTAGGGGATGTATACAACATAGATGTATGGAAGTTTGTTCAGCTAAAGCCATGGTTCGAATAAATGGTAAAT
CCTATATAGACCAAAATAAATGCAGAGAATGTGGATTGTGTAAAAAAGTATGTCCTTACAATGCTATAGTAGAAGTTATG
AGACCATGCAAGAAAGTATGTCCTACAGGAGCTTTAGAAATAAATCCAGATGATAAAAGAGCTATGATAGAAAAGGAAAA
TTGCATTAACTGTGGAGCCTGTATGGCGGCTTGTCCATTTGGAGCCATTTCAGACAAGAGTTATATAGTTAATATAGCTA
AACTATTGAAAGAAAAGAAGAAAGTCTATGCAGTAGTAGCTCCAGCTATCACAGGGCAATTTGGTCCACAAGTAAAAGTT
GGACAAGTAAAGAATGCATTAACAAAGGCAGGGTTTAAAGATATGGTAGAGGCTGCTTGTGGAGCAGATGCAGTAGTATT
TAGTGAATCTGAAGAATTTGTAGAAAGGATGAAAGAGGGACAAAAATGTATGACTACATCTTGCTGTCCAGGCTTTGTGA
ATTATATAGAAAATCAATTTAAAGATTTAGCAGAAAATATATCTACTACGGTATCCCCTATGGTAGCTACCGGAAGAATG
ATAAAAGAGATGGATAAAGATTCATATGTAGTATTTATAGGACCATGCACTGCTAAAAAGAGTGAAGTTCTAAGAAAGGA
ATTAAAGGATGCAGTAGACTATGTTATGACTTTTGAAGAAATAGCGGCCCTTTTAGGTGCACTACAAATAGAGCCTTCTG
AATGTGAAGAAGATCCAGTTCAAGATGCTTCAACTTTTGGAAGAGGATTTGCTCAAAGTGGTGGTGTTACAGCTGCAATA
CAAAACTTTATTGATTCTAAAGAAATAGATATAGAATTTAATCCAACTAAGGTTAGTGGTAAAGATGAAATAAAGAAAAC
CCTTATGTTGGCTAAGGCAGGAAGAATTCCATTTAACTTTGTAGAAGGAATGATGTGTGAAGGAGGATGTATTGGAGGCC
CAGCAACTATGACTTCACTTATGAAAGCTAAAGGCCAATTAATTAAATTTAGTAAAGAATCTGAAAGCAAAGATGTTATA
ACAAATAAAAAAGTAGAAAATTTTGAAAAAGTAAACATGCATAAGCATAAGTAA

Upstream 100 bases:

>100_bases
TTAGAATTTTAAGTTTTTTGTATATTTTACATATGTAAAATATATGTATAAATTACAAAATAATATTTAATATAAAATTT
GAAAATATGGAGGATGCTTG

Downstream 100 bases:

>100_bases
CAGAAAATATAGAATTAAAATAAAAAGTAGTTTTAGTTAAAATATAGCTATAAAAATAAAGTATTTTAAATTATTTTTAT
AGCTATATTTATTTTTTTAA

Product: [Fe] hydrogenase

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLKFETELKKIKYLVLKEVAQLAIEDNMDKLEMLKIPYKILNEKQAEYRCCVYRERTVVYERAQLAAGFKPDDNLTNELE
SISDNDQIIYVIASACDQCPLNRFRVTEACRGCIQHRCMEVCSAKAMVRINGKSYIDQNKCRECGLCKKVCPYNAIVEVM
RPCKKVCPTGALEINPDDKRAMIEKENCINCGACMAACPFGAISDKSYIVNIAKLLKEKKKVYAVVAPAITGQFGPQVKV
GQVKNALTKAGFKDMVEAACGADAVVFSESEEFVERMKEGQKCMTTSCCPGFVNYIENQFKDLAENISTTVSPMVATGRM
IKEMDKDSYVVFIGPCTAKKSEVLRKELKDAVDYVMTFEEIAALLGALQIEPSECEEDPVQDASTFGRGFAQSGGVTAAI
QNFIDSKEIDIEFNPTKVSGKDEIKKTLMLAKAGRIPFNFVEGMMCEGGCIGGPATMTSLMKAKGQLIKFSKESESKDVI
TNKKVENFEKVNMHKHK

Sequences:

>Translated_497_residues
MLKFETELKKIKYLVLKEVAQLAIEDNMDKLEMLKIPYKILNEKQAEYRCCVYRERTVVYERAQLAAGFKPDDNLTNELE
SISDNDQIIYVIASACDQCPLNRFRVTEACRGCIQHRCMEVCSAKAMVRINGKSYIDQNKCRECGLCKKVCPYNAIVEVM
RPCKKVCPTGALEINPDDKRAMIEKENCINCGACMAACPFGAISDKSYIVNIAKLLKEKKKVYAVVAPAITGQFGPQVKV
GQVKNALTKAGFKDMVEAACGADAVVFSESEEFVERMKEGQKCMTTSCCPGFVNYIENQFKDLAENISTTVSPMVATGRM
IKEMDKDSYVVFIGPCTAKKSEVLRKELKDAVDYVMTFEEIAALLGALQIEPSECEEDPVQDASTFGRGFAQSGGVTAAI
QNFIDSKEIDIEFNPTKVSGKDEIKKTLMLAKAGRIPFNFVEGMMCEGGCIGGPATMTSLMKAKGQLIKFSKESESKDVI
TNKKVENFEKVNMHKHK
>Mature_497_residues
MLKFETELKKIKYLVLKEVAQLAIEDNMDKLEMLKIPYKILNEKQAEYRCCVYRERTVVYERAQLAAGFKPDDNLTNELE
SISDNDQIIYVIASACDQCPLNRFRVTEACRGCIQHRCMEVCSAKAMVRINGKSYIDQNKCRECGLCKKVCPYNAIVEVM
RPCKKVCPTGALEINPDDKRAMIEKENCINCGACMAACPFGAISDKSYIVNIAKLLKEKKKVYAVVAPAITGQFGPQVKV
GQVKNALTKAGFKDMVEAACGADAVVFSESEEFVERMKEGQKCMTTSCCPGFVNYIENQFKDLAENISTTVSPMVATGRM
IKEMDKDSYVVFIGPCTAKKSEVLRKELKDAVDYVMTFEEIAALLGALQIEPSECEEDPVQDASTFGRGFAQSGGVTAAI
QNFIDSKEIDIEFNPTKVSGKDEIKKTLMLAKAGRIPFNFVEGMMCEGGCIGGPATMTSLMKAKGQLIKFSKESESKDVI
TNKKVENFEKVNMHKHK

Specific function: Unknown

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 55237; Mature: 55237

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
9.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFETELKKIKYLVLKEVAQLAIEDNMDKLEMLKIPYKILNEKQAEYRCCVYRERTVVY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHH
ERAQLAAGFKPDDNLTNELESISDNDQIIYVIASACDQCPLNRFRVTEACRGCIQHRCME
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHH
VCSAKAMVRINGKSYIDQNKCRECGLCKKVCPYNAIVEVMRPCKKVCPTGALEINPDDKR
HHCCCEEEEECCCHHCCHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCHH
AMIEKENCINCGACMAACPFGAISDKSYIVNIAKLLKEKKKVYAVVAPAITGQFGPQVKV
HHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCEEH
GQVKNALTKAGFKDMVEAACGADAVVFSESEEFVERMKEGQKCMTTSCCPGFVNYIENQF
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHH
KDLAENISTTVSPMVATGRMIKEMDKDSYVVFIGPCTAKKSEVLRKELKDAVDYVMTFEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
IAALLGALQIEPSECEEDPVQDASTFGRGFAQSGGVTAAIQNFIDSKEIDIEFNPTKVSG
HHHHHHHHCCCHHHCCCCCHHHHHHHCCCHHCCCCHHHHHHHHCCCCCEEEEECCCCCCC
KDEIKKTLMLAKAGRIPFNFVEGMMCEGGCIGGPATMTSLMKAKGQLIKFSKESESKDVI
HHHHHHHHHHHHCCCCCHHHHHCHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHH
TNKKVENFEKVNMHKHK
HCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKFETELKKIKYLVLKEVAQLAIEDNMDKLEMLKIPYKILNEKQAEYRCCVYRERTVVY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHH
ERAQLAAGFKPDDNLTNELESISDNDQIIYVIASACDQCPLNRFRVTEACRGCIQHRCME
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHH
VCSAKAMVRINGKSYIDQNKCRECGLCKKVCPYNAIVEVMRPCKKVCPTGALEINPDDKR
HHCCCEEEEECCCHHCCHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCHH
AMIEKENCINCGACMAACPFGAISDKSYIVNIAKLLKEKKKVYAVVAPAITGQFGPQVKV
HHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCEEH
GQVKNALTKAGFKDMVEAACGADAVVFSESEEFVERMKEGQKCMTTSCCPGFVNYIENQF
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHH
KDLAENISTTVSPMVATGRMIKEMDKDSYVVFIGPCTAKKSEVLRKELKDAVDYVMTFEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
IAALLGALQIEPSECEEDPVQDASTFGRGFAQSGGVTAAIQNFIDSKEIDIEFNPTKVSG
HHHHHHHHCCCHHHCCCCCHHHHHHHCCCHHCCCCHHHHHHHHCCCCCEEEEECCCCCCC
KDEIKKTLMLAKAGRIPFNFVEGMMCEGGCIGGPATMTSLMKAKGQLIKFSKESESKDVI
HHHHHHHHHHHHCCCCCHHHHHCHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHH
TNKKVENFEKVNMHKHK
HCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]