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The map label for this gene is vanS [H]

Identifier: 153931588

GI number: 153931588

Start: 1716059

End: 1718161

Strand: Reverse

Name: vanS [H]

Synonym: CLB_1630

Alternate gene names: 153931588

Gene position: 1718161-1716059 (Counterclockwise)

Preceding gene: 153933899

Following gene: 153932392

Centisome position: 44.47

GC content: 23.68

Gene sequence:

>2103_bases
ATGCTGTCTCTTTCATTTTTAACCGGCTATGATTTAATAAAAAACAAAAGGTATCTTGTGAAAGCCCCTTATTTTAATAA
CCCTGAATTTGACATGGAAATATATTCTTATTGTTCAAGCTTGTATAACTTTCATATAACCTATAAAAATTTTGATTATA
AAGTAGCAGAAAATAAGGTTACTAAAGAACAATTAGCAGACTTAAAACTATTTTATGAAGATATGATAAGAAATTCTCAA
GAAGATATTGGAAATAAGTATATTAGTATTTTATCAACAGCTGCACAATCTGATGATAAAGATAAATTTACTAAGTTAAC
ACAAGAAAAAAATAAAGAACTTAAAGAAGTAGAAAAAGAAAATACTAAAACTGAGGCTGAATTAAAAAAAGAAATTGCAT
TATGGTCTTACAATGACTACAAAAATATAAAAAAAGCTATAGAATCTAAAAAAGAGATAAAATACTATATTAAAAACAGC
TTAACCAAAGAAGTTTATACTAATTTAGCACCTAAAACTAATATAGATAGTTATATAAAAAATAATTCTATTTATTCTAT
AAGTTTTCCATTAAAATCTTATAATACTAAAAATTTTTTAGAAACCAATAATTTACTTAATTCTTTTAACTGGGAAGGAA
ATATTATAATTACAAAGGATTTTAATTCTGATGGTTATATATTTAAAAATTATAATTATTATAATTCCATTAGAGATAGG
CTTGTAAAAGAAATAATCATAGGCGTTCCCTCTTTAATTATGGGAATTTTTATGTTAATTATTTTTAAAAGAAGAAATTA
TTTAAATTCTCCTATTTTAAATAAAGTAAAAGAAATGTATAATAATATACCCTTAGATTTAAAACTATTCATATTTGTTT
TATATACCGCCATAATAGGGAGTTATTTAATAAATCTTAGTTTTTTTTATAAACCCTTAGGAATTAATCACTTTGTTATT
ATTACTTTAGTAAGTCTTTATATTCTATATTTAATATTTAATGTAAAATATGCTATAGGACTAAAGAAAGATAAAAAGCA
GTTTTTAATTCAATGTAAGGGAAGTTTCATTAATAAATTATTGAATGCTTTTAAAAATAGTAATTTGAATAAAAATATTA
AATTAAAAATAGCTTTTATTATGGCATTATCTTCTATATTAGCTGCATTTGTACTTTTAGTATTGCTACTAGGAATTCAT
AATATAATAGTTGCATCTATTGTAGCTATTATTTATATAGTTTTGCTTATTAGATACTTATTTAAAAAGGCTGATTATTT
AAATGAAATATTAAAAGCTACAGAAGAAATGTCCTATGGAAATTTAGATTATGTTATAAAGGAAAATGGAGAAAGCGCTC
TAGGTAAAATAGCCCATAATATCAATAATATGAAAGATGGTTATAAAAAATCTCTAGAAGAGCAGGTTAAAAGTGAAAGA
TTAAAAACTGAACTTATAACCAATGTATCTCATGATTTAAAAACTCCCCTTACCTCTATAATAAATTATATAGATTTACT
TAAAAAAGAGGATTTAAGTAAAGATGAAATTAATGGATATATTTCTGTTCTAGATAGAAAGTCTAAAAGACTTAAATCAC
TTATAGAGGATTTATTTGAGGCATCAAAAATGTCCAGCGGTGCAGTAGAATTAAATATTGAAAAAATAAATGTAACAGCT
TTATTAAAGCAATCCATAGCAGAATTTGAAGAAAAAATCACGAAATCCTCTCTAGAACTTAAATTTAAATATGATAATAA
TAAAACCTATGCAAATTTAGATGGAAAGAAAACCTGGAGAGTATTTGAAAATTTAATAAACAATATTATAAAGTATTCTC
AACCTAATACTAGAGTATATATAGATTTAATAGAAACAAATACTAAAATAATAATTACTATGAAAAATATCTCACGCTAT
GAAATGGATTTTAGTGCTGATGAAATTTTTGAAAGATTTAAAAGGGGAGATAAGGCTAGAAACACAGATGGATCTGGGCT
AGGTCTTGCCATTGCAAAAAGTATTTCAGAACTTCAGGATGGGAGCTTAAACATTATCATTGATGGGGATCTTTTTAAGG
TTATTGTAGAGTTTAACAAATAA

Upstream 100 bases:

>100_bases
AACTCTAGAGAACCAAGATATTTAAAGGTGGTGTGGGGAATTGGTTATAAAATCCAAGAATAAATTTATTTATATAATAT
GTTTCATTGTAGGTATATAT

Downstream 100 bases:

>100_bases
TATATTTAACTTTGATTCCACTAATATAAAAAATAAGATTATTTATATATTATTCTAATATTTTATAACTATTCTCAAAA
ATATATAATTTATAACTTTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSSLYNFHITYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
EDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELKKEIALWSYNDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIGSYLINLSFFYKPLGINHFVI
ITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKLLNAFKNSNLNKNIKLKIAFIMALSSILAAFVLLVLLLGIH
NIIVASIVAIIYIVLLIRYLFKKADYLNEILKATEEMSYGNLDYVIKENGESALGKIAHNINNMKDGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFEASKMSSGAVELNIEKINVTA
LLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQDGSLNIIIDGDLFKVIVEFNK

Sequences:

>Translated_700_residues
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSSLYNFHITYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
EDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELKKEIALWSYNDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIGSYLINLSFFYKPLGINHFVI
ITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKLLNAFKNSNLNKNIKLKIAFIMALSSILAAFVLLVLLLGIH
NIIVASIVAIIYIVLLIRYLFKKADYLNEILKATEEMSYGNLDYVIKENGESALGKIAHNINNMKDGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFEASKMSSGAVELNIEKINVTA
LLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQDGSLNIIIDGDLFKVIVEFNK
>Mature_700_residues
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSSLYNFHITYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
EDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELKKEIALWSYNDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIGSYLINLSFFYKPLGINHFVI
ITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKLLNAFKNSNLNKNIKLKIAFIMALSSILAAFVLLVLLLGIH
NIIVASIVAIIYIVLLIRYLFKKADYLNEILKATEEMSYGNLDYVIKENGESALGKIAHNINNMKDGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFEASKMSSGAVELNIEKINVTA
LLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQDGSLNIIIDGDLFKVIVEFNK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=256, Percent_Identity=23.828125, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1787894, Length=285, Percent_Identity=24.2105263157895, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786600, Length=211, Percent_Identity=26.0663507109005, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788713, Length=215, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=24.3137254901961, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=23.728813559322, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=23.7068965517241, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=254, Percent_Identity=25.1968503937008, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=25.6849315068493, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082128, Length=306, Percent_Identity=25.8169934640523, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81167; Mature: 81167

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSSLYNFHITYKNFDYKVAENKV
CCEEHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHEEEEEEECCCEEECCCHH
TKEQLADLKLFYEDMIRNSQEDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NTKTEAELKKEIALWSYNDYKNIKKAIESKKEIKYYIKNSLTKEVYTNLAPKTNIDSYIK
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
NNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHH
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIG
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SYLINLSFFYKPLGINHFVIITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LNAFKNSNLNKNIKLKIAFIMALSSILAAFVLLVLLLGIHNIIVASIVAIIYIVLLIRYL
HHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKADYLNEILKATEEMSYGNLDYVIKENGESALGKIAHNINNMKDGYKKSLEEQVKSER
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKMSSGAVELNIEKINVTALLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWR
HHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCHHHHH
VFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRYEMDFSADEIFERFKRGDKAR
HHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCC
NTDGSGLGLAIAKSISELQDGSLNIIIDGDLFKVIVEFNK
CCCCCCHHHHHHHHHHHHCCCCEEEEECCHHEEHHEECCC
>Mature Secondary Structure
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSSLYNFHITYKNFDYKVAENKV
CCEEHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHEEEEEEECCCEEECCCHH
TKEQLADLKLFYEDMIRNSQEDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NTKTEAELKKEIALWSYNDYKNIKKAIESKKEIKYYIKNSLTKEVYTNLAPKTNIDSYIK
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
NNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHH
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIG
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SYLINLSFFYKPLGINHFVIITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LNAFKNSNLNKNIKLKIAFIMALSSILAAFVLLVLLLGIHNIIVASIVAIIYIVLLIRYL
HHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKADYLNEILKATEEMSYGNLDYVIKENGESALGKIAHNINNMKDGYKKSLEEQVKSER
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKMSSGAVELNIEKINVTALLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWR
HHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCHHHHH
VFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRYEMDFSADEIFERFKRGDKAR
HHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCC
NTDGSGLGLAIAKSISELQDGSLNIIIDGDLFKVIVEFNK
CCCCCCHHHHHHHHHHHHCCCCEEEEECCHHEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]