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The map label for this gene is purF-2 [H]

Identifier: 153931311

GI number: 153931311

Start: 3036560

End: 3037936

Strand: Reverse

Name: purF-2 [H]

Synonym: CLB_2875

Alternate gene names: 153931311

Gene position: 3037936-3036560 (Counterclockwise)

Preceding gene: 153931761

Following gene: 153934268

Centisome position: 78.63

GC content: 28.1

Gene sequence:

>1377_bases
ATGTTATTTGATAGAAAGCAGGATAAATTTAGGGAAGAATGTGGAGTTTTTGGCGTTTTTAAAGATTATACATCAGAACT
TGGAGAAATATTTTATCCAGGGTTAGTATCTCTTCAACATAGAGGAGAAGAAAGTTCAGGAATAAGCTATACAACTAGCA
AAGGTATGATAACTAAAAAAGCTCTTGGAATGGTGTCTAACTTGTTTTCTAAAGAGGATTTTTATAAAATGAAATATTTT
TCTGCTATAGGACATGTTAGATATTCAACTAGTGGTAATGCTTCTATTGAAAATGCACAACCATTTCAAGAAGAAACTAT
AGATGGATCTATTTCTTTAGCTCATAATGGAAATTTATTAAATTATTTAAATATTAAGTATGAATTAGAGAAAAAAGGAA
CGATATTTAAAAGTAATTCAGACTCTGAAATTATACTAAAGTTTATATTAGAAAAAATTCAAGAAGTAAGAGAAATAGAA
AAAGCCATAGCTTATGCTATTAATACTTTAAAGGGAGCTTTTTCAGTACTTATTTTAACCGAAGATAAGTTAATAGGTTT
TAGAGATAAAAATGGTATAAGACCTTTATGTCTAGGAAAAATAGAAGGCAATTATATTTTATCTTCAGAAAGTACTTCCA
TAGATGTTGTGGGAGGAGAATACATAAGAGATGTAGATCCAGGGGAAATAGTGGTTATAAATAAAAAAGGAATAAAATTT
ATAAAGAATAAAGAAGTTTATTGTGGTTCTCTCTGCGCATTAGAATATATATATTTTTCACGACCAGATAGTATCATAGA
TGGGATAAATCTATCACAATTTAGAATTAAATGTGGAGAAAAGTTATATGAAAAATACAAATCAAACTCAGATATTGTAA
TGGGAGTACCTGAATCGGGAAATTTTGCAGCTTTAGGTTATTCTAAGGAATCAAATATACCCTATAGTATAGGTCTTATT
AAAAATTCTTATGTAGGGAGAAATTTTATAAAAGCTACAGAAAAGGAAAGAAAAAGGGATATAAATATAAAAATAAATGC
CATAAAAAGTATAGTTCAAGATAAAAGCATTATTGTGATAGATGATTCTATTGTAAGAGGCACTAGTAGTAAAAAGGTAG
TGTCCGCATTAAAAAAAGCAGGGGCAAGAGAGGTTCACTTTATGGTGGCATCTCCTAAAATAAATTATTATTGTAATTTG
GGTATAGATATAAAAAGTAAAAAAGAGCTTCTATCTTTCCAAAAAACAAAGGAAGAAATGAGAAAGTTTATAGGGGCAGA
TAGCTTAGAATTTTTAAGTTTAATAGATATGGAACAGTGTTTAAATAAATCTAACATTTGTACAGGTTGTTTTGATGGAA
GTTATGCTAATTATTAA

Upstream 100 bases:

>100_bases
TATAGTAAATTATATGTAGTATAAAAAGCTTACCTTCGGGTGGGCTTTATTTTTTTATTTTTAAATTATAATTTGATGTT
TTAAGTTAGAAGGGATGCAA

Downstream 100 bases:

>100_bases
ATTTCATAGCTAGTATAGTATACATAAAAATTTATAATATAAAATAAATTTAATTAGTTTTTAACATTGATTTCTTTTTA
CTTTCTAGAGGTAATATTTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMKYF
SAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIE
KAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESGNFAALGYSKESNIPYSIGLI
KNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNL
GIDIKSKKELLSFQKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY

Sequences:

>Translated_458_residues
MLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMKYF
SAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIE
KAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESGNFAALGYSKESNIPYSIGLI
KNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNL
GIDIKSKKELLSFQKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
>Mature_458_residues
MLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMKYF
SAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIE
KAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESGNFAALGYSKESNIPYSIGLI
KNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNL
GIDIKSKKELLSFQKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=34.1222879684418, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1788651, Length=465, Percent_Identity=35.2688172043011, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=26.6375545851528, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=35.2201257861635, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17539970, Length=188, Percent_Identity=28.7234042553192, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=470, Percent_Identity=34.2553191489362, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28573187, Length=487, Percent_Identity=35.523613963039, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=35.4037267080745, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=35.2297592997812, Blast_Score=304, Evalue=7e-83,
Organism=Drosophila melanogaster, GI21357745, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51448; Mature: 51448

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKK
CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCHHHH
ALGMVSNLFSKEDFYKMKYFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLL
HHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCEE
NYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIEKAIAYAINTLKGAFSVLILT
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
EDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
CCCEEEEECCCCCCCEEEEEECCCEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCCEE
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESG
EECCCCEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
NFAALGYSKESNIPYSIGLIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVI
CEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEEE
DDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNLGIDIKSKKELLSFQKTKEEM
ECHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECEEECCHHHHHHHHHHHHHH
RKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
HHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKK
CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCHHHH
ALGMVSNLFSKEDFYKMKYFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLL
HHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCEE
NYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIEKAIAYAINTLKGAFSVLILT
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
EDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
CCCEEEEECCCCCCCEEEEEECCCEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCCEE
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESG
EECCCCEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
NFAALGYSKESNIPYSIGLIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVI
CEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEEE
DDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNLGIDIKSKKELLSFQKTKEEM
ECHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECEEECCHHHHHHHHHHHHHH
RKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
HHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]