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The map label for this gene is ribE [H]

Identifier: 153931182

GI number: 153931182

Start: 2979057

End: 2979743

Strand: Reverse

Name: ribE [H]

Synonym: CLB_2831

Alternate gene names: 153931182

Gene position: 2979743-2979057 (Counterclockwise)

Preceding gene: 153933315

Following gene: 153934032

Centisome position: 77.13

GC content: 28.24

Gene sequence:

>687_bases
TTGAAGGCTATGTATATTAGAAAAGAGGGTGTTAGTTTGTTTACTGGCATAGTTGAGGAAGTCGGAAGAATATTAAAAAT
AGAGGAAGGAAAAGACTTTCTTCACATCACCATAGAAGGAAGTAAGGTTTTAAATCCATTAAATTTAGGAGAGAGTGTAG
CAGTGAATGGAGTATGTCTTACGGTAACTAGTTTTAATAGTAATAGTTTTACAGCAGATGTTATGACAGAAACTCTAAAT
AAAAGTAGCTTAGGAACATTATCTAAAGGAAGTTTAGTAAATTTGGAAAGAGCTGTAACTTTAAATAAACCTTTAGGTGG
ACATATAGTTTCTGGACATATAGATGGAACAGGGGAAATAATAAATATGAAAAAAGAAGGTATTGCTACATTATTAGAAA
TAAAAGCAAAGGAAAACTTACTAAAATATATGATACCTAAGGGATCTGTAGCATTAGATGGTGTAAGCTTAACTTTAGTG
GATATAAAAGAAAAAAGTTTTACAGTATCTTTAATACCTCATACAAAAGAAGAAACCATATTAGTAAACAAAAATATAGG
TGACATAATAAATATAGAATGCGATGTTCTAGGAAAATATATATATAAGTTTATGCATTTTAAAAAAGAAGAAGCTCTAA
AAAATAATATAAGTTTAGATTTTTTAAGCAAGACAGGATTTTTATAA

Upstream 100 bases:

>100_bases
GATTCAAAAAATATGATAGGTGGAAATGGGGTAGAAAGTTTAAAAGATATATTTAATCTACAAAACTTAAAATTTGAAAA
AATAGGGCAGGATATACTTA

Downstream 100 bases:

>100_bases
TTTAAAATTAACCTTGATTTATTAATGAATATTAGCTTAATGTACAATTTATATTATGTCTAAAAAATAGCTAAAAATAT
AAATAAAAAATTATTATTTT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKAMYIRKEGVSLFTGIVEEVGRILKIEEGKDFLHITIEGSKVLNPLNLGESVAVNGVCLTVTSFNSNSFTADVMTETLN
KSSLGTLSKGSLVNLERAVTLNKPLGGHIVSGHIDGTGEIINMKKEGIATLLEIKAKENLLKYMIPKGSVALDGVSLTLV
DIKEKSFTVSLIPHTKEETILVNKNIGDIINIECDVLGKYIYKFMHFKKEEALKNNISLDFLSKTGFL

Sequences:

>Translated_228_residues
MKAMYIRKEGVSLFTGIVEEVGRILKIEEGKDFLHITIEGSKVLNPLNLGESVAVNGVCLTVTSFNSNSFTADVMTETLN
KSSLGTLSKGSLVNLERAVTLNKPLGGHIVSGHIDGTGEIINMKKEGIATLLEIKAKENLLKYMIPKGSVALDGVSLTLV
DIKEKSFTVSLIPHTKEETILVNKNIGDIINIECDVLGKYIYKFMHFKKEEALKNNISLDFLSKTGFL
>Mature_228_residues
MKAMYIRKEGVSLFTGIVEEVGRILKIEEGKDFLHITIEGSKVLNPLNLGESVAVNGVCLTVTSFNSNSFTADVMTETLN
KSSLGTLSKGSLVNLERAVTLNKPLGGHIVSGHIDGTGEIINMKKEGIATLLEIKAKENLLKYMIPKGSVALDGVSLTLV
DIKEKSFTVSLIPHTKEETILVNKNIGDIINIECDVLGKYIYKFMHFKKEEALKNNISLDFLSKTGFL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=39.4594594594595, Blast_Score=127, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=38.9162561576355, Blast_Score=141, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24944; Mature: 24944

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMYIRKEGVSLFTGIVEEVGRILKIEEGKDFLHITIEGSKVLNPLNLGESVAVNGVCL
CCEEEEECCCHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCCCCCCEEECCEEE
TVTSFNSNSFTADVMTETLNKSSLGTLSKGSLVNLERAVTLNKPLGGHIVSGHIDGTGEI
EEEECCCCCEEHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCEE
INMKKEGIATLLEIKAKENLLKYMIPKGSVALDGVSLTLVDIKEKSFTVSLIPHTKEETI
EEECHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEEEECCCCEEEEECCCCCCCEE
LVNKNIGDIINIECDVLGKYIYKFMHFKKEEALKNNISLDFLSKTGFL
EEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MKAMYIRKEGVSLFTGIVEEVGRILKIEEGKDFLHITIEGSKVLNPLNLGESVAVNGVCL
CCEEEEECCCHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCCCCCCEEECCEEE
TVTSFNSNSFTADVMTETLNKSSLGTLSKGSLVNLERAVTLNKPLGGHIVSGHIDGTGEI
EEEECCCCCEEHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCEE
INMKKEGIATLLEIKAKENLLKYMIPKGSVALDGVSLTLVDIKEKSFTVSLIPHTKEETI
EEECHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEEEECCCCEEEEECCCCCCCEE
LVNKNIGDIINIECDVLGKYIYKFMHFKKEEALKNNISLDFLSKTGFL
EEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]