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The map label for this gene is rumA-1 [C]

Identifier: 153931008

GI number: 153931008

Start: 2971585

End: 2972937

Strand: Reverse

Name: rumA-1 [C]

Synonym: CLB_2825

Alternate gene names: 153931008

Gene position: 2972937-2971585 (Counterclockwise)

Preceding gene: 153933472

Following gene: 153933585

Centisome position: 76.95

GC content: 30.6

Gene sequence:

>1353_bases
ATGAGAAAAGGCAAGGAGTATGAATTTCTTATAGAAGAAACAGAATTTCCGGGAACAGGTGTAGCACAAAAGGATGGTGT
GCCAGTTTATATAAAAGGAACAGTACCAGGACAAAAGGTTTTAGCGAAAGTAACTAAAAAAAGAAGAGAATATGCCCAGG
CTAAACTTTTAGAAATAATAGAAAATATAGATTATGCAATAGAAAATAAATGTCCTCATTTTGGTCAATGTGGAGGGTGT
TCAACCCAATACATACCTTATGAAAAACAACTACAAATTAAAGAAGAACAGCTGTTAAAATTATTTAAAAGTAAAGAAAT
AAAAGGATTTGATTTTTTAGGAGTAGAAAAAAGCCCAGAGGAATACGAATATAGAAACAAGATGGAGTTTACCTTTGGGG
ATATGGAAAAGGGCGGAGATCTTACTTTAGGAATGCATGTTAAAAATAGAAATTTTTCTATTGTAACTGTGGATAACTGT
GAAATTGTAGATAGAGATTTTAGAAATATATTAACAACTGTGGTTAATTATTTTAATGAAAAAAGATTACCTAAATACAG
AGTAATGAGTCATGAAGGTTTTTTAAGAAATTTAGTTATAAGAAAAGCTAAAAATACAGGAGAGATACTAGTAAACATAG
TAACTACATCTCAAATGGAATTTGATTTTAAAGAAATAGTAGATATGTTATTAAAGGTGGAATGCAAAGGAGAAATAAAA
GGCATTCTTCATACTATAAATGATACATTATCTGATGTAGTTCAGGTGGATAAATTAGAAATATTATATGGAAGAGACTA
TATAATAGAAGAGTTATTAGGACTAAAATTTAAGATAGCTCCAGAAGCCTTTTTTCAAACAAATTCAAAGGGAGCAGAAA
AACTATATAGTATAGTAAAAGACTTCTTAGGTGATGCTAGTTCAAAAGTGGTTTTCGACCTTTATTGTGGTACAGGAACC
ATAGGACAAATAGTTGCTCCAAAGGCTAAAAAAGTTATAGGTGTGGAATTAATAGAAGAAGCAGTTAAGGCAGCTAATGA
AAATGCAAAATTAAATGGACTAAATAACTGTGAATTTATAGCTGGTGATGTAGCAAAGGTTATAAAAGATGTAAAACAAA
AGCCAGATATAATTATATTGGATCCACCAAGACCAGGTGTACATCCAGTAGCTTTAGAATATGTGGTTAAGTTTGAGCCA
AAGGAAATAATCTATGTATCTTGTAATCCAAAGACTTTAGTAGATGATTTAAAATATCTTATAGATAATGGGTATAAATT
AGAAAAAGTTAAGGGCATGGACATGTTTCCTCATACACCTCATGTGGAGACTGTGGTTAAGCTAAGTAAGTAA

Upstream 100 bases:

>100_bases
GTAGCTACAGAAAATGGTATTAATAGTATAGCAATAAAATAAAATCTAATAAATAAAATACTTAAAATAAATTTACTCTG
AAAGGATGAAAAATAAATAT

Downstream 100 bases:

>100_bases
TATCAATGGTATAATTAATTTTAAACTATAAAAATCATTCATTTGCCACCCGTTTGCCACCGCTTTTTTATGCTTGGTGG
CAAACTATTTTATTTTAGAT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFGQCGGC
STQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNC
EIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVECKGEIK
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLYCGTGT
IGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEP
KEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETVVKLSK

Sequences:

>Translated_450_residues
MRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFGQCGGC
STQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNC
EIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVECKGEIK
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLYCGTGT
IGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEP
KEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETVVKLSK
>Mature_450_residues
MRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFGQCGGC
STQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNC
EIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVECKGEIK
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLYCGTGT
IGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEP
KEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETVVKLSK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=356, Percent_Identity=29.7752808988764, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI51173878, Length=356, Percent_Identity=29.7752808988764, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI269784680, Length=362, Percent_Identity=29.2817679558011, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI269784676, Length=362, Percent_Identity=29.2817679558011, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI20149688, Length=362, Percent_Identity=29.2817679558011, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI269784678, Length=356, Percent_Identity=29.2134831460674, Blast_Score=146, Evalue=5e-35,
Organism=Escherichia coli, GI1789148, Length=447, Percent_Identity=27.9642058165548, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787083, Length=378, Percent_Identity=25.6613756613757, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790403, Length=230, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71997438, Length=551, Percent_Identity=25.7713248638838, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322909, Length=410, Percent_Identity=27.3170731707317, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24666579, Length=428, Percent_Identity=28.9719626168224, Blast_Score=145, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51062; Mature: 51062

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEII
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPE
HHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCH
EYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNCEIVDRDFRNILTTVVNYFNE
HHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
KRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVECKGEIK
CCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVK
HHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHHHHHHH
DFLGDASSKVVFDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFI
HHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHH
AGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVDDLKYL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEEEEEECCCEEEEEECCCHHHHHHHHHH
IDNGYKLEKVKGMDMFPHTPHVETVVKLSK
HCCCEEEEEECCCCCCCCCCCHHHHHEECC
>Mature Secondary Structure
MRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEII
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPE
HHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCH
EYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNCEIVDRDFRNILTTVVNYFNE
HHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
KRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVECKGEIK
CCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVK
HHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHHHHHHH
DFLGDASSKVVFDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFI
HHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHH
AGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVDDLKYL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEEEEEECCCEEEEEECCCHHHHHHHHHH
IDNGYKLEKVKGMDMFPHTPHVETVVKLSK
HCCCEEEEEECCCCCCCCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]