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The map label for this gene is yqiR [H]

Identifier: 153930959

GI number: 153930959

Start: 3379775

End: 3381403

Strand: Reverse

Name: yqiR [H]

Synonym: CLB_3232

Alternate gene names: 153930959

Gene position: 3381403-3379775 (Counterclockwise)

Preceding gene: 153933492

Following gene: 153932075

Centisome position: 87.52

GC content: 25.17

Gene sequence:

>1629_bases
ATGTTTATGGAAGAAGACTTTAAAATTATAAGTGAAAATATTAACAAGGGTACAATTAATATCAATAATAAAAATAATGT
ACTATCTGATAAATATTTATTAAAAAATAAAAGTAATAATAGGCATGTGGATATATTAAAAAATAAAGAAACTCATAAAA
AGATTAATAAAAATTCTAAGGTAGAAAGTAAAATAAGTATAAATGAATCAGAATGTGCAACTACTATAGATTATAAACTA
TACAGCGAAATGAAAAAAGATATAAAATATTATAAATCAGTTTTAGATGTTGTTAATAAAGTACTATATACAATAAACGA
ATGTATAGTAGTTGTGGATCAAAATGGAATAATTATAATGATGAGTGAATGCTATAAAAAGTTTATTAATTGCAAAGATC
CAGAGGGAAAATATGTTGGAGATGTTATAAAGGATACTATGTTACATAGAGTTTTAAAAACAGGGAACATAGAAAGTGGA
GAAGTACAAAAAATTAATGGCAAACAAGTAATTACTATGAGGATACCATTGAAAAAAGAAGGTAGAATTATTGGAGCAAT
AGGCAAAATAATGTTTAAAGATGTGAATGATTTTTATTTTTTATATGGAAAGTTAAATAAGTCAAAAAATACACATAAAT
GTTACGATAATAAATCAGAAAAAAATAGACGAGCTAAATATTTTTTTAAGGATATTGTTGGAGATTCTTCTGAAAGTAAT
AATATTAAAAGTTTAGCTAAGAAATCTGCAAATACAAATTCGAATGTTTTAATAGTAGGAGAAAGTGGAACAGGTAAAGA
ATTATATGCTCATTCAATTCATAATGCTAGTAATAGAAGATTAGGGCCTTTTATAAAAATTAACTGTGGGGCTATACCAG
CTGAATTGTTAGAGTCTGAATTATTTGGATATGAAGATGGTGCTTTTACAGGAGCTAAAAAAGGGGGGAAAAAAGGGAAA
TTTGAGTTGGCAAATGGAGGTACTATATTTTTAGATGAAATTGGTGATATGCCAATGTGTATGCAAGTTAAGCTTCTAAG
AGTCATTCAAGAAAGAGAAGTAGAAAAAGTGGGGAGCAATATACTAAAGAAAATTGATGTTAGGATAATTGCAGCTAGAA
ATAAAAATCTTGAAAAGTTAGTCGAAAAAAATAAATTTAGAGAGGATTTATATTATAGATTGAATGTTATAAGGATTAAT
GTTCCATCACTAAGAGAAAGAAAAGAAGATATATTATTAATATCAGATTTTTTAGTAAAAAAAGTATCTCATAGATTAGG
CATATGTATAAAAGAAATATCTAAAGAAGCTAAAGAATATTTATGTAATTATAATTGGCCAGGAAATATTAGGGAGCTGG
AGAATGTAATAGAACGATCTGCAAATTTATTAGACTCAGATTTTGTTATAAAACCAAAACATTTACCAAAAGAAATATTA
AAAGGTTATATTGTAAAAGCCCCCACTAAAGAAAATAAATATTTAAAAGACATAATTTCGGAAGTAGAAAAAGAAGTTAT
TTGTAACTGTTTAGAAAAAAATAATGGAAATAAAAATAAAACCTCTAAGATATTAGGAATAAGTAGAGCAAATTTGTATA
AGAAAATATCAGAATATAATATAGTTTAA

Upstream 100 bases:

>100_bases
TTTTAACAATGTAAAGATATGTATTATAAAAGAATATTTGTCAAATATTGCATCAAGGTATATAATGAAGTTATAGGTAA
TTACTTAGGGGGTATATTCT

Downstream 100 bases:

>100_bases
CTTGATAAAATATAAAAACAACAGAACACTTAATAATGGATCTGTTGTTTTTTATTTTATTTTATTTTTTAAGAAGATGT
TTTTTTATATTCTAAGGTAT

Product: sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MFMEEDFKIISENINKGTININNKNNVLSDKYLLKNKSNNRHVDILKNKETHKKINKNSKVESKISINESECATTIDYKL
YSEMKKDIKYYKSVLDVVNKVLYTINECIVVVDQNGIIIMMSECYKKFINCKDPEGKYVGDVIKDTMLHRVLKTGNIESG
EVQKINGKQVITMRIPLKKEGRIIGAIGKIMFKDVNDFYFLYGKLNKSKNTHKCYDNKSEKNRRAKYFFKDIVGDSSESN
NIKSLAKKSANTNSNVLIVGESGTGKELYAHSIHNASNRRLGPFIKINCGAIPAELLESELFGYEDGAFTGAKKGGKKGK
FELANGGTIFLDEIGDMPMCMQVKLLRVIQEREVEKVGSNILKKIDVRIIAARNKNLEKLVEKNKFREDLYYRLNVIRIN
VPSLRERKEDILLISDFLVKKVSHRLGICIKEISKEAKEYLCNYNWPGNIRELENVIERSANLLDSDFVIKPKHLPKEIL
KGYIVKAPTKENKYLKDIISEVEKEVICNCLEKNNGNKNKTSKILGISRANLYKKISEYNIV

Sequences:

>Translated_542_residues
MFMEEDFKIISENINKGTININNKNNVLSDKYLLKNKSNNRHVDILKNKETHKKINKNSKVESKISINESECATTIDYKL
YSEMKKDIKYYKSVLDVVNKVLYTINECIVVVDQNGIIIMMSECYKKFINCKDPEGKYVGDVIKDTMLHRVLKTGNIESG
EVQKINGKQVITMRIPLKKEGRIIGAIGKIMFKDVNDFYFLYGKLNKSKNTHKCYDNKSEKNRRAKYFFKDIVGDSSESN
NIKSLAKKSANTNSNVLIVGESGTGKELYAHSIHNASNRRLGPFIKINCGAIPAELLESELFGYEDGAFTGAKKGGKKGK
FELANGGTIFLDEIGDMPMCMQVKLLRVIQEREVEKVGSNILKKIDVRIIAARNKNLEKLVEKNKFREDLYYRLNVIRIN
VPSLRERKEDILLISDFLVKKVSHRLGICIKEISKEAKEYLCNYNWPGNIRELENVIERSANLLDSDFVIKPKHLPKEIL
KGYIVKAPTKENKYLKDIISEVEKEVICNCLEKNNGNKNKTSKILGISRANLYKKISEYNIV
>Mature_542_residues
MFMEEDFKIISENINKGTININNKNNVLSDKYLLKNKSNNRHVDILKNKETHKKINKNSKVESKISINESECATTIDYKL
YSEMKKDIKYYKSVLDVVNKVLYTINECIVVVDQNGIIIMMSECYKKFINCKDPEGKYVGDVIKDTMLHRVLKTGNIESG
EVQKINGKQVITMRIPLKKEGRIIGAIGKIMFKDVNDFYFLYGKLNKSKNTHKCYDNKSEKNRRAKYFFKDIVGDSSESN
NIKSLAKKSANTNSNVLIVGESGTGKELYAHSIHNASNRRLGPFIKINCGAIPAELLESELFGYEDGAFTGAKKGGKKGK
FELANGGTIFLDEIGDMPMCMQVKLLRVIQEREVEKVGSNILKKIDVRIIAARNKNLEKLVEKNKFREDLYYRLNVIRIN
VPSLRERKEDILLISDFLVKKVSHRLGICIKEISKEAKEYLCNYNWPGNIRELENVIERSANLLDSDFVIKPKHLPKEIL
KGYIVKAPTKENKYLKDIISEVEKEVICNCLEKNNGNKNKTSKILGISRANLYKKISEYNIV

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=47.1875, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1790437, Length=341, Percent_Identity=43.9882697947214, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1789233, Length=340, Percent_Identity=42.6470588235294, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786524, Length=367, Percent_Identity=36.2397820163488, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789087, Length=319, Percent_Identity=44.2006269592476, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788905, Length=385, Percent_Identity=38.7012987012987, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=37.6506024096386, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=41.2844036697248, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87082152, Length=310, Percent_Identity=37.0967741935484, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1787583, Length=357, Percent_Identity=35.0140056022409, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=38.5852090032154, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI87081858, Length=327, Percent_Identity=31.1926605504587, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=33.0472103004292, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62043; Mature: 62043

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMEEDFKIISENINKGTININNKNNVLSDKYLLKNKSNNRHVDILKNKETHKKINKNSK
CCCCHHHHHHHCCCCCCEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHCCCCC
VESKISINESECATTIDYKLYSEMKKDIKYYKSVLDVVNKVLYTINECIVVVDQNGIIIM
CCCEEECCHHHHHEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEE
MSECYKKFINCKDPEGKYVGDVIKDTMLHRVLKTGNIESGEVQKINGKQVITMRIPLKKE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCC
GRIIGAIGKIMFKDVNDFYFLYGKLNKSKNTHKCYDNKSEKNRRAKYFFKDIVGDSSESN
CCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCC
NIKSLAKKSANTNSNVLIVGESGTGKELYAHSIHNASNRRLGPFIKINCGAIPAELLESE
HHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
LFGYEDGAFTGAKKGGKKGKFELANGGTIFLDEIGDMPMCMQVKLLRVIQEREVEKVGSN
HCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILKKIDVRIIAARNKNLEKLVEKNKFREDLYYRLNVIRINVPSLRERKEDILLISDFLVK
HHHHHCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEEECCHHHHCHHCEEHHHHHHHH
KVSHRLGICIKEISKEAKEYLCNYNWPGNIRELENVIERSANLLDSDFVIKPKHLPKEIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHH
KGYIVKAPTKENKYLKDIISEVEKEVICNCLEKNNGNKNKTSKILGISRANLYKKISEYN
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECHHHHHHHHHHHCC
IV
CC
>Mature Secondary Structure
MFMEEDFKIISENINKGTININNKNNVLSDKYLLKNKSNNRHVDILKNKETHKKINKNSK
CCCCHHHHHHHCCCCCCEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHCCCCC
VESKISINESECATTIDYKLYSEMKKDIKYYKSVLDVVNKVLYTINECIVVVDQNGIIIM
CCCEEECCHHHHHEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEE
MSECYKKFINCKDPEGKYVGDVIKDTMLHRVLKTGNIESGEVQKINGKQVITMRIPLKKE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCC
GRIIGAIGKIMFKDVNDFYFLYGKLNKSKNTHKCYDNKSEKNRRAKYFFKDIVGDSSESN
CCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCC
NIKSLAKKSANTNSNVLIVGESGTGKELYAHSIHNASNRRLGPFIKINCGAIPAELLESE
HHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
LFGYEDGAFTGAKKGGKKGKFELANGGTIFLDEIGDMPMCMQVKLLRVIQEREVEKVGSN
HCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILKKIDVRIIAARNKNLEKLVEKNKFREDLYYRLNVIRINVPSLRERKEDILLISDFLVK
HHHHHCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEEECCHHHHCHHCEEHHHHHHHH
KVSHRLGICIKEISKEAKEYLCNYNWPGNIRELENVIERSANLLDSDFVIKPKHLPKEIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHH
KGYIVKAPTKENKYLKDIISEVEKEVICNCLEKNNGNKNKTSKILGISRANLYKKISEYN
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECHHHHHHHHHHHCC
IV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]