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The map label for this gene is thiO/thiG [H]

Identifier: 153003020

GI number: 153003020

Start: 172032

End: 173159

Strand: Reverse

Name: thiO/thiG [H]

Synonym: Anae109_0143

Alternate gene names: 153003020

Gene position: 173159-172032 (Counterclockwise)

Preceding gene: 153003021

Following gene: 153003019

Centisome position: 3.28

GC content: 77.3

Gene sequence:

>1128_bases
TTGCCCGCCATGCGGACGGACGTGGTGGTGGTGGGCGCCGGCATCCAGGGCGCCTCGGTCGCGCTGCGGCTCGCGCAGGC
GGGGCGCGACGTGGTGGTGCTCGAGCGCTCGATCCCGGGCGCCGAGGCGTCGAGCGCGGCGGGCGGCATCCTCTCTCCTG
GCGTCGAGGCGATCGAGCCCGGCCCGTTCTACGCGCTCTGCCGCGCCTCGCTCGCGCGCTACCCGGAGTTCGTGCGCGAG
GTCGAGGCGCGCTCCGGGATCGCGGTGGGCTACCGCGCGCTCGGCACGCTGGAGGTCGCGCTCGACGACGATCACGCGCG
GCTCCTCGCGGGCAGGGCCGAGAAGCTGGAGCGCAGCGGCCTGCCCGTGGAGGTGCTCGACGAGGCCGCCGTCCGCGCCC
TCGAGCCGGGCGTCTCCTCAGAGGCGCGCGGCGCCCTCTACTTCGCCGACGAGGCGTCGCTCGATCCGCGCCTGCTCGCG
CGCGCGGTGTACGTCGCCGCGGCGCGCGCCGGCGCGCGGTTCGTGACGGGGGACGTGCGCCGCATCCGCACGGAGGGCGG
CCGCGCCGCGGGCGTCGACCACGCGGCAGGGCGGATCGACGCCGGGGCGGTGGTGCTCGCCGCGGGGAGCTGGAGCCTCC
TCGTGGAGGGAAACGGCCTCCCGCCGGGCGCGGTGCGCCCGGTGCGCGGTCAGATCGCCGTCGTGGACACTCGGCCGCCG
CTCCTCTCCCGCGTGGTCTTCTCGGGCAACGGCTACGTGGTGCCCCGACCCGACGGCCGGATCCTGTGCGGCTCCACCAT
GGAGGACGTGGGCTGGGAGAAGGCGGTCACCGCGGGCGGGCTCCGCCACGTCCTCGGCGTCGCTGTCGAGATCGCGCCCG
CGCTCGCCCAGGCGCCGGTCGTGGAGACCTGGTCGAACTTCCGGCCCGCGAGCCCGGACGGCGAACCCATCCTCGGCGAG
GGGACGGTCCCCGGCCTGTTCTACGCCACCGGCCACACCCGCAACGGGATCCTCCTCGCGCCCGTCACCGCGGACGCGAT
CGCCGCCGCCGTCACCGGCGCCGCCGCGCCGCTCGACCTCGCGCCCTTCTCGCCCGCGCGGCTCGGCGCGATCCTCGGGC
GGCGCTGA

Upstream 100 bases:

>100_bases
TCCGTCCCCGGGACGCCGGGCGGCACGCCGAGCAACGGCGCAGCCGTGTGATCATCGTGAGCCCTGCGCGCTTCCCCCGC
GAGGCCGCGAGGGCGTAGTC

Downstream 100 bases:

>100_bases
GCCAGCCGCGAGGCGCGTGGCGCGCACCGGGCGTGCGTAGCTTCTCGTGGGATGGAGGCCCCCGCCGACCTGCACGAGCT
GAGGGAGCGGCTGCTCGCCC

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MPAMRTDVVVVGAGIQGASVALRLAQAGRDVVVLERSIPGAEASSAAGGILSPGVEAIEPGPFYALCRASLARYPEFVRE
VEARSGIAVGYRALGTLEVALDDDHARLLAGRAEKLERSGLPVEVLDEAAVRALEPGVSSEARGALYFADEASLDPRLLA
RAVYVAAARAGARFVTGDVRRIRTEGGRAAGVDHAAGRIDAGAVVLAAGSWSLLVEGNGLPPGAVRPVRGQIAVVDTRPP
LLSRVVFSGNGYVVPRPDGRILCGSTMEDVGWEKAVTAGGLRHVLGVAVEIAPALAQAPVVETWSNFRPASPDGEPILGE
GTVPGLFYATGHTRNGILLAPVTADAIAAAVTGAAAPLDLAPFSPARLGAILGRR

Sequences:

>Translated_375_residues
MPAMRTDVVVVGAGIQGASVALRLAQAGRDVVVLERSIPGAEASSAAGGILSPGVEAIEPGPFYALCRASLARYPEFVRE
VEARSGIAVGYRALGTLEVALDDDHARLLAGRAEKLERSGLPVEVLDEAAVRALEPGVSSEARGALYFADEASLDPRLLA
RAVYVAAARAGARFVTGDVRRIRTEGGRAAGVDHAAGRIDAGAVVLAAGSWSLLVEGNGLPPGAVRPVRGQIAVVDTRPP
LLSRVVFSGNGYVVPRPDGRILCGSTMEDVGWEKAVTAGGLRHVLGVAVEIAPALAQAPVVETWSNFRPASPDGEPILGE
GTVPGLFYATGHTRNGILLAPVTADAIAAAVTGAAAPLDLAPFSPARLGAILGRR
>Mature_374_residues
PAMRTDVVVVGAGIQGASVALRLAQAGRDVVVLERSIPGAEASSAAGGILSPGVEAIEPGPFYALCRASLARYPEFVREV
EARSGIAVGYRALGTLEVALDDDHARLLAGRAEKLERSGLPVEVLDEAAVRALEPGVSSEARGALYFADEASLDPRLLAR
AVYVAAARAGARFVTGDVRRIRTEGGRAAGVDHAAGRIDAGAVVLAAGSWSLLVEGNGLPPGAVRPVRGQIAVVDTRPPL
LSRVVFSGNGYVVPRPDGRILCGSTMEDVGWEKAVTAGGLRHVLGVAVEIAPALAQAPVVETWSNFRPASPDGEPILGEG
TVPGLFYATGHTRNGILLAPVTADAIAAAVTGAAAPLDLAPFSPARLGAILGRR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=431, Percent_Identity=24.1299303944316, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 38449; Mature: 38318

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAMRTDVVVVGAGIQGASVALRLAQAGRDVVVLERSIPGAEASSAAGGILSPGVEAIEP
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCC
GPFYALCRASLARYPEFVREVEARSGIAVGYRALGTLEVALDDDHARLLAGRAEKLERSG
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHCC
LPVEVLDEAAVRALEPGVSSEARGALYFADEASLDPRLLARAVYVAAARAGARFVTGDVR
CCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECHHH
RIRTEGGRAAGVDHAAGRIDAGAVVLAAGSWSLLVEGNGLPPGAVRPVRGQIAVVDTRPP
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCH
LLSRVVFSGNGYVVPRPDGRILCGSTMEDVGWEKAVTAGGLRHVLGVAVEIAPALAQAPV
HHHEEEECCCCEEEECCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VETWSNFRPASPDGEPILGEGTVPGLFYATGHTRNGILLAPVTADAIAAAVTGAAAPLDL
HHHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCCCCC
APFSPARLGAILGRR
CCCCHHHHHHHHCCC
>Mature Secondary Structure 
PAMRTDVVVVGAGIQGASVALRLAQAGRDVVVLERSIPGAEASSAAGGILSPGVEAIEP
CCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCC
GPFYALCRASLARYPEFVREVEARSGIAVGYRALGTLEVALDDDHARLLAGRAEKLERSG
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHCC
LPVEVLDEAAVRALEPGVSSEARGALYFADEASLDPRLLARAVYVAAARAGARFVTGDVR
CCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECHHH
RIRTEGGRAAGVDHAAGRIDAGAVVLAAGSWSLLVEGNGLPPGAVRPVRGQIAVVDTRPP
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCH
LLSRVVFSGNGYVVPRPDGRILCGSTMEDVGWEKAVTAGGLRHVLGVAVEIAPALAQAPV
HHHEEEECCCCEEEECCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VETWSNFRPASPDGEPILGEGTVPGLFYATGHTRNGILLAPVTADAIAAAVTGAAAPLDL
HHHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCCCCC
APFSPARLGAILGRR
CCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]