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The map label for this gene is ispE

Identifier: 153003004

GI number: 153003004

Start: 153628

End: 154545

Strand: Reverse

Name: ispE

Synonym: Anae109_0127

Alternate gene names: 153003004

Gene position: 154545-153628 (Counterclockwise)

Preceding gene: 153003006

Following gene: 153003003

Centisome position: 2.93

GC content: 74.95

Gene sequence:

>918_bases
GTGCGCCTCCAGACGATCGCCCCGGCCAAGGTGAACCTCGTGCTGCGCGTCGGCCCGCCGCGCAGGGACGGCTATCACGG
CATCCGCTCGCTGATGGTCCCCCTCGACCTCGGCGATCGCGTGGACGTGTCGGTGGGCGAGCGCGCCGGCCCCGTCACCT
GCCGGGTCCCGGGGAGGCCGGAGCTCGACGGCGCCGACAACCTCGCCGCGCGCGCCGCCGAGCGCTTCCGCGCGCGCTTC
GGCGTCGAGCGGACGATCGGCATCCGGATCGCGAAGCGCATCCCGGTGACCGCGGGGCTCGGCGGCGGGTCCTCCGACGC
CGCGGCGGTGCTGCGCTGCCTCGCTCGCGCGTTCGGGATCCGCGACCGCGCCGCGCTCGCCGCGATCGCCCTCGAGGTCG
GCTCCGACGTGCCCTTCTTCCTCGGCCCGGGGCCGGCGTGGGCCGAGGGCCGGGGCGAGAGGCTCACGCCCGCCGACGTG
CCCCCGCAGCACCTCGTCCTCGTCTACCCCGACGATCCGGCGCTCGCGATCCGGGCCGGCGAGGCCTACCGCTGGCTCGA
CGCGTCGCGGCAGGGCCGCAGGCCGCGTCTGCCGCCGCGCTTCGCGCGCTTCGAGCCATCCCGCGCGGGGAACGATCTCC
AGCCGCCCTGCCTGCTGGAGCGGCCGCCCCTGGCGACGCTCTTGGGGCTGCTTGTGGGTCGAGGCGCGACGGCCGCTATA
ATGTCGGGAAGTGGGCCCACCGTGTTCGGCACGTTTCACAGCCGGAACGCGGCTGCGCGGGCGGCGCGGACGATCGCGGA
GGAGGTCGTCGATCGGAAGGTGATGGTCCTCGCCGCGACGACGGTGCGACGGCATCCGGGGGTTACGCCATGGAGATCAC
CGAGGTCCGCGTCTTCCCCGTCAACGAAGAGAAGCTGA

Upstream 100 bases:

>100_bases
GCAACGCATCAGAGGAGCGGGAGCGACGTTCGTGACGCGGCCCGGGCGGACGCGCGCGGACCGCCGCGCCGCGCTCGCGC
GGGCGTGGTAGGAGCGTCCC

Downstream 100 bases:

>100_bases
AGGCCTACGTCACCATCACCCTCGACGACTGCTTCGTCGTCCGCGACCTCAAGGTCATCCACGGGAACACCGGCCTCTTC
ATCGCGATGCCGGCGAAGCG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRLQTIAPAKVNLVLRVGPPRRDGYHGIRSLMVPLDLGDRVDVSVGERAGPVTCRVPGRPELDGADNLAARAAERFRARF
GVERTIGIRIAKRIPVTAGLGGGSSDAAAVLRCLARAFGIRDRAALAAIALEVGSDVPFFLGPGPAWAEGRGERLTPADV
PPQHLVLVYPDDPALAIRAGEAYRWLDASRQGRRPRLPPRFARFEPSRAGNDLQPPCLLERPPLATLLGLLVGRGATAAI
MSGSGPTVFGTFHSRNAAARAARTIAEEVVDRKVMVLAATTVRRHPGVTPWRSPRSASSPSTKRS

Sequences:

>Translated_305_residues
MRLQTIAPAKVNLVLRVGPPRRDGYHGIRSLMVPLDLGDRVDVSVGERAGPVTCRVPGRPELDGADNLAARAAERFRARF
GVERTIGIRIAKRIPVTAGLGGGSSDAAAVLRCLARAFGIRDRAALAAIALEVGSDVPFFLGPGPAWAEGRGERLTPADV
PPQHLVLVYPDDPALAIRAGEAYRWLDASRQGRRPRLPPRFARFEPSRAGNDLQPPCLLERPPLATLLGLLVGRGATAAI
MSGSGPTVFGTFHSRNAAARAARTIAEEVVDRKVMVLAATTVRRHPGVTPWRSPRSASSPSTKRS
>Mature_305_residues
MRLQTIAPAKVNLVLRVGPPRRDGYHGIRSLMVPLDLGDRVDVSVGERAGPVTCRVPGRPELDGADNLAARAAERFRARF
GVERTIGIRIAKRIPVTAGLGGGSSDAAAVLRCLARAFGIRDRAALAAIALEVGSDVPFFLGPGPAWAEGRGERLTPADV
PPQHLVLVYPDDPALAIRAGEAYRWLDASRQGRRPRLPPRFARFEPSRAGNDLQPPCLLERPPLATLLGLLVGRGATAAI
MSGSGPTVFGTFHSRNAAARAARTIAEEVVDRKVMVLAATTVRRHPGVTPWRSPRSASSPSTKRS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=177, Percent_Identity=36.7231638418079, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ANADF (A7H6J9)

Other databases:

- EMBL:   CP000769
- RefSeq:   YP_001377329.1
- ProteinModelPortal:   A7H6J9
- STRING:   A7H6J9
- GeneID:   5378222
- GenomeReviews:   CP000769_GR
- KEGG:   afw:Anae109_0127
- NMPDR:   fig|404589.4.peg.120
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   CLSK944838
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32560; Mature: 32560

Theoretical pI: Translated: 11.89; Mature: 11.89

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQTIAPAKVNLVLRVGPPRRDGYHGIRSLMVPLDLGDRVDVSVGERAGPVTCRVPGRP
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHEEECCCCCCEECCCCCCCCCEEEECCCCC
ELDGADNLAARAAERFRARFGVERTIGIRIAKRIPVTAGLGGGSSDAAAVLRCLARAFGI
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHCC
RDRAALAAIALEVGSDVPFFLGPGPAWAEGRGERLTPADVPPQHLVLVYPDDPALAIRAG
CHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECC
EAYRWLDASRQGRRPRLPPRFARFEPSRAGNDLQPPCLLERPPLATLLGLLVGRGATAAI
CCEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEE
MSGSGPTVFGTFHSRNAAARAARTIAEEVVDRKVMVLAATTVRRHPGVTPWRSPRSASSP
ECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCC
STKRS
CCCCC
>Mature Secondary Structure
MRLQTIAPAKVNLVLRVGPPRRDGYHGIRSLMVPLDLGDRVDVSVGERAGPVTCRVPGRP
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHEEECCCCCCEECCCCCCCCCEEEECCCCC
ELDGADNLAARAAERFRARFGVERTIGIRIAKRIPVTAGLGGGSSDAAAVLRCLARAFGI
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHCC
RDRAALAAIALEVGSDVPFFLGPGPAWAEGRGERLTPADVPPQHLVLVYPDDPALAIRAG
CHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECC
EAYRWLDASRQGRRPRLPPRFARFEPSRAGNDLQPPCLLERPPLATLLGLLVGRGATAAI
CCEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEE
MSGSGPTVFGTFHSRNAAARAARTIAEEVVDRKVMVLAATTVRRHPGVTPWRSPRSASSP
ECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCC
STKRS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA