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The map label for this gene is rimK [H]

Identifier: 153002891

GI number: 153002891

Start: 14588

End: 15577

Strand: Reverse

Name: rimK [H]

Synonym: Anae109_0012

Alternate gene names: 153002891

Gene position: 15577-14588 (Counterclockwise)

Preceding gene: 153002903

Following gene: 153002890

Centisome position: 0.3

GC content: 72.73

Gene sequence:

>990_bases
ATGCCGCCCCCCCGCAAGATCGGAGCGCGCCCGCGCGCGCGGCGCCGCCGCGTGTCCGCCTCGACGGAGGGGCTCGGCGT
CGTCGTGCTCTCCCGCAACCCGAAGCTCTACTCGACGCGGCGGCTCGTCGAGGCGGTGGGCGCGCTCGGCCACCTGCCGC
GCGTGCTCGACACGCTCCGCTGCAACATGGTGCTCGGCCGCGACCGTCCGCGGATGTTCTACGCCGGCGAGGAGCTCCTC
GCGACCGACGTCCACGTGGCCATCCCCCGCATCGGCGCGTCCATCACCGGCTACGGCCTCTCGGTGGTGAACCAGCTCGA
CATGATGGGCGTGCCGGTGCTGAACAACGCCATCCCCATCGCCCGCTCGCGCGACAAGCTGCGCGCGCTGCAGCTCCTCT
CGCGCTTCGGCCTCGACATCCCGCGCACGGTGATGTGCCGCTACCGCGACGAGGTCCCCCAGGCGGTCGAGATGGTGGGC
GGCCTGCCGTGCATCATCAAGCTCATCCAGGGGACGCAGGGCGTCGGCGTGATGATCGCCCACACCTCGGCGGAGGTGGA
GGGGATGCTCGACACGCTCTGGACGCTGGGTCAGGAGATCCTCTTGCAGGAGCTCGTGGCCGAGTCGCGCGGCAAGGACG
TGCGGGCGCTCGTGGTGGGCGACCACGTGGTCGCCGCCATGCGGCGCACGGCCCGCGCCGGCGAGTTCCGCTCCAACATC
CACCGGGGCGGCGTGGCGGAGGCGGTCGAGCTCGACCGCGAGTTCGCCGAGGTGGCGGTGAAGGCCGCGCGGGTGATGGG
GCTCGAGGTCGCCGGCGTGGACATGCTCGAGGCGCGCACCGGCCCGAAGGTCATGGAGGTCAACTCCTCCCCCGGCTTCG
AGGGGCTCGAGGCGGCCACCGGCGTGGACATCGCCACGGCCTACGTGTCGCACGCCATCGAGTTCGGCCACGCCCGCCTC
TCCGGCAGCACGGGCGGTCGCCTCGTGTGA

Upstream 100 bases:

>100_bases
ACCCGGGTTTTTAGCAGCGCGGGGGAGGACATTCAACGAAGCGGTTCGGCCGGGCGCTCCATTGCGGGCGGAGCGGGGCG
CCGCTAACCTTCCCCGCCTC

Downstream 100 bases:

>100_bases
GCGGAGGTGGGGAGCGGGCAGGCGAGCAGGCCGACCTGCGGACGCCCCTTCACCAAAGCGATCCCGCCCTCTAGAATCCG
CCGCAACCGTGCCACCTCGA

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MPPPRKIGARPRARRRRVSASTEGLGVVVLSRNPKLYSTRRLVEAVGALGHLPRVLDTLRCNMVLGRDRPRMFYAGEELL
ATDVHVAIPRIGASITGYGLSVVNQLDMMGVPVLNNAIPIARSRDKLRALQLLSRFGLDIPRTVMCRYRDEVPQAVEMVG
GLPCIIKLIQGTQGVGVMIAHTSAEVEGMLDTLWTLGQEILLQELVAESRGKDVRALVVGDHVVAAMRRTARAGEFRSNI
HRGGVAEAVELDREFAEVAVKAARVMGLEVAGVDMLEARTGPKVMEVNSSPGFEGLEAATGVDIATAYVSHAIEFGHARL
SGSTGGRLV

Sequences:

>Translated_329_residues
MPPPRKIGARPRARRRRVSASTEGLGVVVLSRNPKLYSTRRLVEAVGALGHLPRVLDTLRCNMVLGRDRPRMFYAGEELL
ATDVHVAIPRIGASITGYGLSVVNQLDMMGVPVLNNAIPIARSRDKLRALQLLSRFGLDIPRTVMCRYRDEVPQAVEMVG
GLPCIIKLIQGTQGVGVMIAHTSAEVEGMLDTLWTLGQEILLQELVAESRGKDVRALVVGDHVVAAMRRTARAGEFRSNI
HRGGVAEAVELDREFAEVAVKAARVMGLEVAGVDMLEARTGPKVMEVNSSPGFEGLEAATGVDIATAYVSHAIEFGHARL
SGSTGGRLV
>Mature_328_residues
PPPRKIGARPRARRRRVSASTEGLGVVVLSRNPKLYSTRRLVEAVGALGHLPRVLDTLRCNMVLGRDRPRMFYAGEELLA
TDVHVAIPRIGASITGYGLSVVNQLDMMGVPVLNNAIPIARSRDKLRALQLLSRFGLDIPRTVMCRYRDEVPQAVEMVGG
LPCIIKLIQGTQGVGVMIAHTSAEVEGMLDTLWTLGQEILLQELVAESRGKDVRALVVGDHVVAAMRRTARAGEFRSNIH
RGGVAEAVELDREFAEVAVKAARVMGLEVAGVDMLEARTGPKVMEVNSSPGFEGLEAATGVDIATAYVSHAIEFGHARLS
GSTGGRLV

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=228, Percent_Identity=24.5614035087719, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI134254462, Length=216, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787076, Length=281, Percent_Identity=47.3309608540925, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006037
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK; PF02080 TrkA_C [H]

EC number: NA

Molecular weight: Translated: 35480; Mature: 35349

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPRKIGARPRARRRRVSASTEGLGVVVLSRNPKLYSTRRLVEAVGALGHLPRVLDTLR
CCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CNMVLGRDRPRMFYAGEELLATDVHVAIPRIGASITGYGLSVVNQLDMMGVPVLNNAIPI
HHHEECCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCC
ARSRDKLRALQLLSRFGLDIPRTVMCRYRDEVPQAVEMVGGLPCIIKLIQGTQGVGVMIA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
HTSAEVEGMLDTLWTLGQEILLQELVAESRGKDVRALVVGDHVVAAMRRTARAGEFRSNI
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
HRGGVAEAVELDREFAEVAVKAARVMGLEVAGVDMLEARTGPKVMEVNSSPGFEGLEAAT
HCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHCCCCCEEEECCCCCCCCHHHHC
GVDIATAYVSHAIEFGHARLSGSTGGRLV
CHHHHHHHHHHHHHHCCCEECCCCCCCCC
>Mature Secondary Structure 
PPPRKIGARPRARRRRVSASTEGLGVVVLSRNPKLYSTRRLVEAVGALGHLPRVLDTLR
CCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CNMVLGRDRPRMFYAGEELLATDVHVAIPRIGASITGYGLSVVNQLDMMGVPVLNNAIPI
HHHEECCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCC
ARSRDKLRALQLLSRFGLDIPRTVMCRYRDEVPQAVEMVGGLPCIIKLIQGTQGVGVMIA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
HTSAEVEGMLDTLWTLGQEILLQELVAESRGKDVRALVVGDHVVAAMRRTARAGEFRSNI
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
HRGGVAEAVELDREFAEVAVKAARVMGLEVAGVDMLEARTGPKVMEVNSSPGFEGLEAAT
HCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHCCCCCEEEECCCCCCCCHHHHC
GVDIATAYVSHAIEFGHARLSGSTGGRLV
CHHHHHHHHHHHHHHCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]