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The map label for this gene is gidB [H]

Identifier: 152977681

GI number: 152977681

Start: 4080643

End: 4081362

Strand: Reverse

Name: gidB [H]

Synonym: Bcer98_4022

Alternate gene names: 152977681

Gene position: 4081362-4080643 (Counterclockwise)

Preceding gene: 152977682

Following gene: 152977680

Centisome position: 99.86

GC content: 36.11

Gene sequence:

>720_bases
ATGAACATAGAACAATTTCAATCTATGCTAGGAGAAAGAGGGATTTCCCTTTCTCCTAGACAATTAGAGCAATTTGAAAT
GTATTTTGAAACATTAGTAGAGTGGAACGAAAAAATGAATTTAACAGCGATTACGGAGAAAGAAGAAGTGTACTTAAAAC
ATTTTTTTGACTCTATTACTGCTGCTTTTTACTACGATTTTTCAAATCCACTATCTATTTGTGATGTTGGCGCAGGTGCA
GGATTTCCGAGTATACCATTAAAAATTTGTTTCCCGCACTTAAAAGTAACAATTGTTGATTCACTGCAAAAGCGGATTAA
CTTTTTGAATCACTTAGCACAAAAACTGGAGCTCAGTGACGTTGCTTTCTGTCATGATCGTGCTGAAACATTCGGTAAAA
AAGAACATGTACGTGAATCATACGATATTGTAATGGCGCGTGCAGTAGCTCGCCTTTCTGTATTAAGTGAATTATGTTTA
CCGCTTGTAAAGGTTGGCGGAACATTTATTGCCATGAAAGGTGCAGCAGCTAATGAAGAGATTGAAAATGGTAAATATGC
TGTAGAAGTGCTTGGCGGACAATTAGAAGAGGTATCTACGTTCCAATTGCCATTTGAAGAGAGTGAACGGAACATTTTAT
TAATCAAGAAAAAGCGCAAAACACCGAAAAAATATCCACGTAAGCCAGGTACACCCAATAAATTACCTATTGAAAAATAA

Upstream 100 bases:

>100_bases
AGGTGTAAATCCAGCTGATATTTCCATTTTACTTGTATACATTGAACAAGGGAAAATTGCGCGAGTATCGAACCAATAAA
TAGTAAGGAGATATTGCGAG

Downstream 100 bases:

>100_bases
ATCTTATTAGACGTAAGATTAAATTATATAAATGGAACCGTAAATGTTTGATAAGGAATATGTTTATGTAGAATATTCAA
TAGGCCCAAAGGTGGTGGAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNIEQFQSMLGERGISLSPRQLEQFEMYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSNPLSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEHVRESYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAANEEIENGKYAVEVLGGQLEEVSTFQLPFEESERNILLIKKKRKTPKKYPRKPGTPNKLPIEK

Sequences:

>Translated_239_residues
MNIEQFQSMLGERGISLSPRQLEQFEMYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSNPLSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEHVRESYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAANEEIENGKYAVEVLGGQLEEVSTFQLPFEESERNILLIKKKRKTPKKYPRKPGTPNKLPIEK
>Mature_239_residues
MNIEQFQSMLGERGISLSPRQLEQFEMYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSNPLSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEHVRESYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAANEEIENGKYAVEVLGGQLEEVSTFQLPFEESERNILLIKKKRKTPKKYPRKPGTPNKLPIEK

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=217, Percent_Identity=32.258064516129, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27181; Mature: 27181

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQFQSMLGERGISLSPRQLEQFEMYFETLVEWNEKMNLTAITEKEEVYLKHFFDSIT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
AAFYYDFSNPLSICDVGAGAGFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSD
HHHHCCCCCCCCCEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFCHDRAETFGKKEHVRESYDIVMARAVARLSVLSELCLPLVKVGGTFIAMKGAAANEE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
IENGKYAVEVLGGQLEEVSTFQLPFEESERNILLIKKKRKTPKKYPRKPGTPNKLPIEK
HCCCCEEEEECCCCHHHHHEEECCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIEQFQSMLGERGISLSPRQLEQFEMYFETLVEWNEKMNLTAITEKEEVYLKHFFDSIT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
AAFYYDFSNPLSICDVGAGAGFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSD
HHHHCCCCCCCCCEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFCHDRAETFGKKEHVRESYDIVMARAVARLSVLSELCLPLVKVGGTFIAMKGAAANEE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
IENGKYAVEVLGGQLEEVSTFQLPFEESERNILLIKKKRKTPKKYPRKPGTPNKLPIEK
HCCCCEEEEECCCCHHHHHEEECCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA