Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 152977666

GI number: 152977666

Start: 4066592

End: 4067881

Strand: Reverse

Name: purA [H]

Synonym: Bcer98_4007

Alternate gene names: 152977666

Gene position: 4067881-4066592 (Counterclockwise)

Preceding gene: 152977667

Following gene: 152977665

Centisome position: 99.53

GC content: 37.6

Gene sequence:

>1290_bases
ATGTCTTCAGTAGTAGTTGTAGGAACACAATGGGGCGATGAAGGAAAAGGTAAAATTACTGACTTTCTTTCTGAACATGC
GGAAGTAGTTGCAAGATATCAAGGTGGAAATAACGCAGGACATACAATCGTTTTTAATGGTGTGAAATATAAATTACACT
TAATTCCATCTGGTATTTTTTATAAAGAGAAGATTTGTGTAATTGGTAATGGTATGGTAGTAGATCCAAAAGCATTACTT
GTAGAATTAAAATACTTACACGATCGCGGTGTAAGTACTGATAATTTACGCATCAGTAATCGCGCGCACGTTATCTTACC
ATATCATTTAAAACAAGATGAGTTAGAAGAAGAGCGTAAGGGCGATAATAAAATTGGTACAACGAAAAAAGGTATCGGCC
CAGCATATATGGATAAAGCAGCTCGCATAGGTATTCGTATGGCGGATCTTTTAGATCGTGAAGCTTTTAAAGAAAAACTG
GAACGCAATTTAGCGGAAAAAAATCGTTTATTTGAAAAAATGTATGATGCAGAAGGCTTTAACGTGGATGAAATCTTTGA
AGAATACTATGAGTATGGTCAACAAATTGCACAATATGTATGCGATACATCTGTTGTATTAAATGATGCATTAGATGAAG
GTCGTCGTGTATTATTTGAAGGTGCACAAGGCGTTATGCTTGATATCGACCAAGGTACGTACCCATTCGTTACATCTTCT
AACCCAGTTGCTGGTGGTGTAACAATTGGTTCTGGTGTGGGTCCTTCTAAAATTAAACGTGTAGTAGGTGTATGTAAAGC
ATATACAAGCCGTGTTGGTGATGGTCCATTCCCTACTGAGCTTCATGATGAAATTGGTCAACAAATTCGTGAAGTTGGTC
GTGAATATGGTACAACAACAGGCCGTCCACGCCGCGTGGGTTGGTTTGATAGTGTTGTTGTAAGACATGCGCGCCGCGTT
AGTGGTTTAACAGATCTTTCTTTAAACTCTATCGACGTATTAACAGGTATTCCAACTGTGAAAATTTGTGTTGCATACAA
ATATAATGGAGAAGTTCTGGATGAAGTTCCAGCTAACTTAAACATTTTAGCAAAATGTGAGCCTGTATACGAAGAACTTC
CAGGTTGGACAGAAGATATTACAGGGGTAAAATCATTAGAAGAACTTCCTGAAAATGCAAGACATTATGTAGAACGTGTA
TCTCAACTAACAGGTATTCCATTAGCTATGTTCTCTGTTGGTCCAGACCGTAGCCAAACAAATATTGTTCGTAATGTATA
TGGCATTTAA

Upstream 100 bases:

>100_bases
ACTTAAAAAAATGTTCGTTTTTAAATTGACTTCTCTTTTCATTTTGGTACAATTATATTGTTTGAAAAGTTCGTTTGAAA
ATCGCGGAGGTGCTTGAATA

Downstream 100 bases:

>100_bases
TTATTGATTTTCATGTAAAGCTACATTAGACTTAGTAAAGAATCCGTGTTTACGCGGATTCTTTTTAACTTTTATAAAAA
AAATTTAAAAAGTATTGTAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFNGVKYKLHLIPSGIFYKEKICVIGNGMVVDPKALL
VELKYLHDRGVSTDNLRISNRAHVILPYHLKQDELEEERKGDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKL
ERNLAEKNRLFEKMYDAEGFNVDEIFEEYYEYGQQIAQYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKIKRVVGVCKAYTSRVGDGPFPTELHDEIGQQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLNSIDVLTGIPTVKICVAYKYNGEVLDEVPANLNILAKCEPVYEELPGWTEDITGVKSLEELPENARHYVERV
SQLTGIPLAMFSVGPDRSQTNIVRNVYGI

Sequences:

>Translated_429_residues
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFNGVKYKLHLIPSGIFYKEKICVIGNGMVVDPKALL
VELKYLHDRGVSTDNLRISNRAHVILPYHLKQDELEEERKGDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKL
ERNLAEKNRLFEKMYDAEGFNVDEIFEEYYEYGQQIAQYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKIKRVVGVCKAYTSRVGDGPFPTELHDEIGQQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLNSIDVLTGIPTVKICVAYKYNGEVLDEVPANLNILAKCEPVYEELPGWTEDITGVKSLEELPENARHYVERV
SQLTGIPLAMFSVGPDRSQTNIVRNVYGI
>Mature_428_residues
SSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFNGVKYKLHLIPSGIFYKEKICVIGNGMVVDPKALLV
ELKYLHDRGVSTDNLRISNRAHVILPYHLKQDELEEERKGDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLE
RNLAEKNRLFEKMYDAEGFNVDEIFEEYYEYGQQIAQYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSSN
PVAGGVTIGSGVGPSKIKRVVGVCKAYTSRVGDGPFPTELHDEIGQQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVS
GLTDLSLNSIDVLTGIPTVKICVAYKYNGEVLDEVPANLNILAKCEPVYEELPGWTEDITGVKSLEELPENARHYVERVS
QLTGIPLAMFSVGPDRSQTNIVRNVYGI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=42.4170616113744, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=42.8909952606635, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=41.8367346938776, Blast_Score=290, Evalue=3e-78,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=47.6635514018692, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=44.0559440559441, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=44.0559440559441, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=41.371158392435, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=44.1247002398081, Blast_Score=343, Evalue=9e-95,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47591; Mature: 47459

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFNGVKYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECEEEEEEEECCCCE
YKEKICVIGNGMVVDPKALLVELKYLHDRGVSTDNLRISNRAHVILPYHLKQDELEEERK
EECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCHHHHHHHHC
GDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLERNLAEKNRLFEKMYDAEGF
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NVDEIFEEYYEYGQQIAQYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKIKRVVGVCKAYTSRVGDGPFPTELHDEIGQQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTVKICVAYKYNGEVLDEVPANL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEEECCCCHHCCCCCC
NILAKCEPVYEELPGWTEDITGVKSLEELPENARHYVERVSQLTGIPLAMFSVGPDRSQT
EEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
NIVRNVYGI
HHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFNGVKYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECEEEEEEEECCCCE
YKEKICVIGNGMVVDPKALLVELKYLHDRGVSTDNLRISNRAHVILPYHLKQDELEEERK
EECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCHHHHHHHHC
GDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLERNLAEKNRLFEKMYDAEGF
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NVDEIFEEYYEYGQQIAQYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKIKRVVGVCKAYTSRVGDGPFPTELHDEIGQQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTVKICVAYKYNGEVLDEVPANL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEEECCCCHHCCCCCC
NILAKCEPVYEELPGWTEDITGVKSLEELPENARHYVERVSQLTGIPLAMFSVGPDRSQT
EEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
NIVRNVYGI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA