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The map label for this gene is metK [H]

Identifier: 152977126

GI number: 152977126

Start: 3497207

End: 3498406

Strand: Reverse

Name: metK [H]

Synonym: Bcer98_3437

Alternate gene names: 152977126

Gene position: 3498406-3497207 (Counterclockwise)

Preceding gene: 152977128

Following gene: 152977123

Centisome position: 85.6

GC content: 40.67

Gene sequence:

>1200_bases
ATGACAAAACAACGTCATCTGTTCACATCTGAGTCTGTAACTGAAGGACATCCAGATAAAATTTGTGACCAAATTTCTGA
TTCAATTTTAGATGCGATCTTATCAAAAGACGCAAATGCACGTGTAGCTTGTGAAACAACAGTAACAACTGGTTTAGTAT
TGGTAGCGGGGGAAATTACAACTTCTACGTATGTAGATATTCCAAAAATCGTTCGTGAAACAATACAAGGCATTGGGTAT
ACTCGTGCAAAATATGGATTCGATGCAGAAACTTGTGCAGTTTTAACATCTATCGATGAACAATCTGCTGACATCGCTAT
GGGTGTTGACCGAGCGTTAGAAGCACGTGAAGGTCAAATGACTGATGCTGAGATTGAAGCGATTGGTGCAGGAGACCAAG
GTTTAATGTTTGGTTTCGCATGTAATGAAACGAAAGAATTAATGCCACTTCCAATCTCACTTGCTCACAAATTAGCTCGT
CGTTTAACGGAAGTTCGTAAAGATGAAACATTAGAATATTTACGTCCAGACGGTAAAACGCAAGTTACAGTTGAGTATGA
TGAAAATGGTAAACCAGTACGTGTCGACACAATTGTAATTTCTACTCAACATCATCCGGAAATTACATGGGAACAAATCG
ATCGCGACTTAAAAGAGCATGTAATTAAAGCTGTTGTGCCAGCAGAATTAATGGACGAAGAAACAAAATTCTTTATTAAC
CCAACTGGTCGTTTCGTAATTGGTGGGCCACAAGGGGATGCTGGATTAACGGGACGTAAAATTATCGTTGATACTTATGG
TGGATATGCTCGTCACGGCGGTGGTGCATTCTCTGGTAAGGATGCAACAAAAGTTGACCGTTCTGCAGCGTATGCAGCAC
GTTATGTTGCGAAAAATATTGTAGCAGCTGGTCTTGCTGAAAAAGCAGAAGTACAGCTTGCGTATGCAATTGGTGTAGCG
AAGCCAGTATCAATTTCAGTTGATACATTTGGAACTGGGAAAGTATCTGAAGAGGTACTAGTAGAATTAGTTCGTAACAA
CTTCGATCTTCGCCCAGCTGGCATTATTAAAATGTTAGACCTACGTCGCCCAATTTACAAACAAACAGCAGCTTACGGCC
ACTTTGGACGTACTGATGTAGATCTTCCTTGGGAGCGTACAGACAAAGCAGCTGACTTAAAAGAGCAAGCTGGTCTATAA

Upstream 100 bases:

>100_bases
CTTTATACTTGTGTGGGAGAATAAATGAATGTCGCAATTTGTGGCAAGTTAAGGATGAGTTCCGTACAATATATACAATT
ACTGTAGGGAGGTTTACCAC

Downstream 100 bases:

>100_bases
TATAATAAAAAAGGTTTTGCGCTTTCGCGCAAAACCTTTTTTATTTATAGTTAGGCTGTAATAGTGCAAAAAGTTCAATA
TCATGCCATGCCCCATTTCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTKQRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDRALEAREGQMTDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLAR
RLTEVRKDETLEYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPEITWEQIDRDLKEHVIKAVVPAELMDEETKFFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVA
KPVSISVDTFGTGKVSEEVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLPWERTDKAADLKEQAGL

Sequences:

>Translated_399_residues
MTKQRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDRALEAREGQMTDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLAR
RLTEVRKDETLEYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPEITWEQIDRDLKEHVIKAVVPAELMDEETKFFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVA
KPVSISVDTFGTGKVSEEVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLPWERTDKAADLKEQAGL
>Mature_398_residues
TKQRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGYT
RAKYGFDAETCAVLTSIDEQSADIAMGVDRALEAREGQMTDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLARR
LTEVRKDETLEYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPEITWEQIDRDLKEHVIKAVVPAELMDEETKFFINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAK
PVSISVDTFGTGKVSEEVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLPWERTDKAADLKEQAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=59.1623036649215, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=59.4240837696335, Blast_Score=436, Evalue=1e-122,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=64.0506329113924, Blast_Score=506, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=56.2982005141388, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.6122448979592, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=55.2699228791774, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=54.2857142857143, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=50.8997429305913, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=55.0295857988166, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=53.9589442815249, Blast_Score=354, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=54.983922829582, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=52.8735632183908, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=65.9090909090909, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=57.4412532637076, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=55.2162849872774, Blast_Score=426, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=382, Percent_Identity=56.5445026178011, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=382, Percent_Identity=56.5445026178011, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=382, Percent_Identity=56.5445026178011, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=382, Percent_Identity=56.5445026178011, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=382, Percent_Identity=56.5445026178011, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=382, Percent_Identity=56.5445026178011, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=55.4973821989529, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=55.4973821989529, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=54.3243243243243, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=54.3307086614173, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43534; Mature: 43403

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEIT
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCEEEEEECCC
TSTYVDIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDRALEAREGQM
CCHHCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
TDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLARRLTEVRKDETLEYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
QVTVEYDENGKPVRVDTIVISTQHHPEITWEQIDRDLKEHVIKAVVPAELMDEETKFFIN
EEEEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAEVQLAYAIGVAKPVSISVDTFGTGKVSEEVLVELVRNNFDLRPAGIIKMLD
HHHCCCHHHHEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLPWERTDKAADLKEQAGL
HCCHHHHHHHHCCCCCCCCCCCCHHCCCCHHCHHHHCCC
>Mature Secondary Structure 
TKQRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEIT
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCEEEEEECCC
TSTYVDIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDRALEAREGQM
CCHHCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
TDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLARRLTEVRKDETLEYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
QVTVEYDENGKPVRVDTIVISTQHHPEITWEQIDRDLKEHVIKAVVPAELMDEETKFFIN
EEEEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAEVQLAYAIGVAKPVSISVDTFGTGKVSEEVLVELVRNNFDLRPAGIIKMLD
HHHCCCHHHHEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLPWERTDKAADLKEQAGL
HCCHHHHHHHHCCCCCCCCCCCCHHCCCCHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA