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The map label for this gene is ytpA [H]

Identifier: 152977120

GI number: 152977120

Start: 3492903

End: 3493706

Strand: Reverse

Name: ytpA [H]

Synonym: Bcer98_3430

Alternate gene names: 152977120

Gene position: 3493706-3492903 (Counterclockwise)

Preceding gene: 152977122

Following gene: 152977119

Centisome position: 85.48

GC content: 37.44

Gene sequence:

>804_bases
ATGTGGAATTATGAAGCGGAATCGGCAAAGGCTGTTATCGTAATTGTGCATGGTGCCATGGAATACCATGGACGTTATGA
AGCGCTGGCAGAAGAGTGGAATCACATTGGGTATCATGTTGTTATGGGAGATCTTCCTGCTCATGGAACAACGTCAAGAA
ATAGAGGACATATTAATTCGTTTGATGAATACATAGAAGAGGTCAAAGTATGGATAAAAGAAGCGAGAAAGTATAGACTA
CCTATTTTTTTATTGGGACATAGTATGGGGGGACTTATCATCATTCGAATGATGGAAGAAACGAAGCGCGAAGATATAGA
TGGTATTATATTAAGTTCACCATGCTTAGGGGTGTTAGCTACTCCAGCGGCACCACTTCGAGCTCTGGCGAAGGTATTAA
ATATTGTTATGCCGAAATTACAATTTCCGACGAATCTTACAGTGGAAATGTCAACAAGAAATAAAGAAGTTCGCGATGCA
ATGGAAAATGATTCATTGTTCTTGCGCAAGGTATCCGTTCGCTGGTATAGTGAATTGATAAAATCTACACGAATTGCTCA
TGAAAAAATAGATGAGTTCCCAGACGTTCCGCTCTTGCTAATGCAGGCGTGTGAGGATAAACTAGTAGATAAAACAAGTG
TCCGTAAATGGTTTGATAATTTAAAAATCAGTGATAAGGCGTATAAAGAATGGCCGAATTGTTATCATGAGTTATTAAAT
GAGTATGAGCGAGATGAAGTTTTTAATTATATCAAGTCATTTACTGAAATGCATGTCAATAACAGAATAGAAACAAATAA
ATAA

Upstream 100 bases:

>100_bases
TTTTTATCTTTGTAAGAATAGATCATGTTCAGCCTTCCTTTCCTAGAGACTATTTTTAGTGTATCAAATTATGAAACAGA
GTGAAATGGAGGAGAATTAT

Downstream 100 bases:

>100_bases
TGAATTTGTACATTTAATAGAGGAGATGAAGGCCGAAGTGAACGTACCGAGTAATCCCATAACGCTCATGGCGAAAGTAT
ACCGTGATGTGTTTCCGGTA

Product: alpha/beta hydrolase fold

Products: glycerophosphocholine; carboxylate

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MWNYEAESAKAVIVIVHGAMEYHGRYEALAEEWNHIGYHVVMGDLPAHGTTSRNRGHINSFDEYIEEVKVWIKEARKYRL
PIFLLGHSMGGLIIIRMMEETKREDIDGIILSSPCLGVLATPAAPLRALAKVLNIVMPKLQFPTNLTVEMSTRNKEVRDA
MENDSLFLRKVSVRWYSELIKSTRIAHEKIDEFPDVPLLLMQACEDKLVDKTSVRKWFDNLKISDKAYKEWPNCYHELLN
EYERDEVFNYIKSFTEMHVNNRIETNK

Sequences:

>Translated_267_residues
MWNYEAESAKAVIVIVHGAMEYHGRYEALAEEWNHIGYHVVMGDLPAHGTTSRNRGHINSFDEYIEEVKVWIKEARKYRL
PIFLLGHSMGGLIIIRMMEETKREDIDGIILSSPCLGVLATPAAPLRALAKVLNIVMPKLQFPTNLTVEMSTRNKEVRDA
MENDSLFLRKVSVRWYSELIKSTRIAHEKIDEFPDVPLLLMQACEDKLVDKTSVRKWFDNLKISDKAYKEWPNCYHELLN
EYERDEVFNYIKSFTEMHVNNRIETNK
>Mature_267_residues
MWNYEAESAKAVIVIVHGAMEYHGRYEALAEEWNHIGYHVVMGDLPAHGTTSRNRGHINSFDEYIEEVKVWIKEARKYRL
PIFLLGHSMGGLIIIRMMEETKREDIDGIILSSPCLGVLATPAAPLRALAKVLNIVMPKLQFPTNLTVEMSTRNKEVRDA
MENDSLFLRKVSVRWYSELIKSTRIAHEKIDEFPDVPLLLMQACEDKLVDKTSVRKWFDNLKISDKAYKEWPNCYHELLN
EYERDEVFNYIKSFTEMHVNNRIETNK

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI51242953, Length=265, Percent_Identity=32.8301886792453, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI6005786, Length=265, Percent_Identity=32.8301886792453, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322756, Length=268, Percent_Identity=23.5074626865672, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: 3.1.1.5

Molecular weight: Translated: 31117; Mature: 31117

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNYEAESAKAVIVIVHGAMEYHGRYEALAEEWNHIGYHVVMGDLPAHGTTSRNRGHINS
CCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCH
FDEYIEEVKVWIKEARKYRLPIFLLGHSMGGLIIIRMMEETKREDIDGIILSSPCLGVLA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHC
TPAAPLRALAKVLNIVMPKLQFPTNLTVEMSTRNKEVRDAMENDSLFLRKVSVRWYSELI
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
KSTRIAHEKIDEFPDVPLLLMQACEDKLVDKTSVRKWFDNLKISDKAYKEWPNCYHELLN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EYERDEVFNYIKSFTEMHVNNRIETNK
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MWNYEAESAKAVIVIVHGAMEYHGRYEALAEEWNHIGYHVVMGDLPAHGTTSRNRGHINS
CCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCH
FDEYIEEVKVWIKEARKYRLPIFLLGHSMGGLIIIRMMEETKREDIDGIILSSPCLGVLA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHC
TPAAPLRALAKVLNIVMPKLQFPTNLTVEMSTRNKEVRDAMENDSLFLRKVSVRWYSELI
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
KSTRIAHEKIDEFPDVPLLLMQACEDKLVDKTSVRKWFDNLKISDKAYKEWPNCYHELLN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EYERDEVFNYIKSFTEMHVNNRIETNK
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-lysophosphatidylcholine; H2O

Specific reaction: 2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]