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The map label for this gene is narK [H]

Identifier: 152977080

GI number: 152977080

Start: 3455572

End: 3456741

Strand: Reverse

Name: narK [H]

Synonym: Bcer98_3390

Alternate gene names: 152977080

Gene position: 3456741-3455572 (Counterclockwise)

Preceding gene: 152977081

Following gene: 152977079

Centisome position: 84.58

GC content: 34.1

Gene sequence:

>1170_bases
ATGAGAAGTCCGAATTTTCAGTTAGGTTTACAAACTTCTAATCTTATTATTGGTTTTATGGTATGGGTGATTTTATCTTC
GTTAATGCCATTTATTAAAACAGATATTACGTTAACGGCAGGGCAAATCTCCCTTGTAACAGCAGTACCTGTTATTTTAG
GATCGGTGCTTCGAATACCAATTGGGTATTGGACAAACCGATATGGAGCAAGAAAATTATTCTTTATCAGTTTTATTATT
TTACTGTTCCCTGTTTTTTATATTAGTATTGCAAACTCTATGATGGATTTAATTATTGGTGGTCTATTCGTAGGAATTGG
TGGCGCTGTATTTTCTGTAGGTGTGACGTCATTACCGAAATATTATCCAAAAGAACGTCACGGTTTTGTAAATGGTATTT
ATGGGGCGGGAAATGCAGGAACAGCAATTACTTCTTTTTTAGCACCAGTCATTGCAAGTTCATTTGGTTGGAGAGCAACT
GTACAGTCATTTTTAATTTTATTAGCATTGTTTGCGTTATTAAATTTCTTATTAGGTGATCGCAATGAAAGAAAAGTGAA
CACACCATTATTAGAACAATTAAAAGGTGTGTATAAAAATGAGAAACTTTGGTTTTTATGCATTTTCTATTTCTTAACGT
TTGGATCATTTGTTGCTTTTACGGTGTACTTACCAAACTTTTTAGTGTCTCACTTTGGATTAGCAAAAGTCGATGCAGGT
ATGAGAACAGCTGGATTTATTGTATTAGCAACAATTATGCGCCCGATAGGTGGATGGCTTGGTGATAAGTTTAATCCATT
TAAAATTTTAATCTTTGTATTTATTGGATTGACGTTATCGGGTATTATTTTATCATTTATGCCAAGCATGAATGTCTATA
CATTTGGTTGTTTACTCGTTGCGTTTTGTGCAGGAATTGGAAATGGGACAGTCTTTAAACTTGTACCGATGTATTTTTCT
AAGCAAGCAGGGGTTGTAAATGGGCTTGTTGCAGCATTAGGTGGATTAGGTGGATTTTTCCCTCCGCTTATTTTAACAGT
ACTGTTCCAATTAACAGGTCATTATGCAATTGGATTTATGGCACTTTCAGAAGTGGCACTTGCTTGTCTCATTATTACAG
TTTGGATGTATAGTCAAGAAAAGCTATTACTTATGCTAAATAATAAATAA

Upstream 100 bases:

>100_bases
AGGTTGATTTTTTCCAGTCTAATCACGAGATCACGTGATTAGACTGGAGGGAATGAATATAAAATTGTGAAACATTGAAC
AAATTAGGAGGCGGAAAGAG

Downstream 100 bases:

>100_bases
TTCCATTGTTTGAAAAAGAGAGCAGCATGGTTTCAGTTGGAAAACTAGAAGCCTGCTGTTTTTTGTATTGTTTGAAAAGA
TTGAGGGGAATATAAAGGCG

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRSPNFQLGLQTSNLIIGFMVWVILSSLMPFIKTDITLTAGQISLVTAVPVILGSVLRIPIGYWTNRYGARKLFFISFII
LLFPVFYISIANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYYPKERHGFVNGIYGAGNAGTAITSFLAPVIASSFGWRAT
VQSFLILLALFALLNFLLGDRNERKVNTPLLEQLKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLAKVDAG
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTVFKLVPMYFS
KQAGVVNGLVAALGGLGGFFPPLILTVLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLLMLNNK

Sequences:

>Translated_389_residues
MRSPNFQLGLQTSNLIIGFMVWVILSSLMPFIKTDITLTAGQISLVTAVPVILGSVLRIPIGYWTNRYGARKLFFISFII
LLFPVFYISIANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYYPKERHGFVNGIYGAGNAGTAITSFLAPVIASSFGWRAT
VQSFLILLALFALLNFLLGDRNERKVNTPLLEQLKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLAKVDAG
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTVFKLVPMYFS
KQAGVVNGLVAALGGLGGFFPPLILTVLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLLMLNNK
>Mature_389_residues
MRSPNFQLGLQTSNLIIGFMVWVILSSLMPFIKTDITLTAGQISLVTAVPVILGSVLRIPIGYWTNRYGARKLFFISFII
LLFPVFYISIANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYYPKERHGFVNGIYGAGNAGTAITSFLAPVIASSFGWRAT
VQSFLILLALFALLNFLLGDRNERKVNTPLLEQLKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLAKVDAG
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTVFKLVPMYFS
KQAGVVNGLVAALGGLGGFFPPLILTVLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLLMLNNK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=437, Percent_Identity=24.0274599542334, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787743, Length=440, Percent_Identity=20.9090909090909, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786686, Length=264, Percent_Identity=23.4848484848485, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42587; Mature: 42587

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]