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The map label for this gene is tyrS1 [H]

Identifier: 152977028

GI number: 152977028

Start: 3402027

End: 3403283

Strand: Reverse

Name: tyrS1 [H]

Synonym: Bcer98_3333

Alternate gene names: 152977028

Gene position: 3403283-3402027 (Counterclockwise)

Preceding gene: 152977029

Following gene: 152977027

Centisome position: 83.27

GC content: 36.83

Gene sequence:

>1257_bases
ATGAATATTTTACAAGATCTTGAGTTTCGCGGTTTGATTAATCAGCAAACAGATGCTGAAGGTTTAGAACAATTATTAGA
AAAAGAAAGCGTTAAATTATACTGTGGTTTTGACCCAACAGCAGATAGCTTACACATCGGACATATGTTACCGATTTTAA
TGTTGCGTCGCTTTCAATTAGCTGGTCATCAACCAATCGCACTTGTAGGTGGTGGTACTGGTATGATCGGGGACCCAAGT
GGTAAAAAAGCAGAGCGTACATTAAATACGAAGGATACAGTTGCTTACTACACTGAAAGTATTAAAAAACAGCTTTCAAA
CTTCTTAGAATTTGAAAATGTAGAAAATCCAGCAGTAATGGCAAATAACTACGACTGGCTTGGCAACTTAGATGTTATTA
CATTCTTACGTGACATCGGTAAAAACTTCGGTTTAAACTACATGTTAGCAAAAGATACAGTTGCATCTCGTTTAGAAACT
GGTATTTCATTTACGGAATTTAGTTACATGATTTTACAATCATATGACTTCTTAAACTTATATAAAAACCATGGTTGCCG
TCTGCAAATTGGTGGTAGTGACCAATGGGGTAACATTACAGCGGGACTTGAATTAATCCGTAAATCGGAAGAAGATGCGA
AAGCATTCGGGTTAACAATTCCACTTGTTACAAAATCTGACGGTACGAAGTTTGGAAAAACAGAAGGCGGTGCAGTTTGG
TTAGACCCAGAGAAAACAACTCCTTACGAGTTCTATCAATTCTGGATTAACACAGATGACCGCGATGTTGTGAAATATTT
AAAATACTTCACATTCCTATCTCATGAAGAAATTCTTGATTTAGAAAAACAAGTAGCTGAAGCACCTGAAAAACGTGTAG
CACAAAAAGCATTAGCTTCTGAAATGACAAAGCTTGTTCACGGCGAAGAAGCATTAGAACAAGCAATTAAAATTTCTGCA
GCTTTATTTAGTGGTTCTGTAGCAGAATTAACAGCAGATGAAATTGAACAAGGCTTCAAAGATGTACCATCTGTAGACCG
CAGCGCAGAAGATACAGGGTTAATTGACTTACTTGTAGAAAGCAAAATTTCTCCATCAAAACGTCAAGCACGTGAAGATG
TTACAAACGGTGCGATTTATATAAACGGTGAGCGCACACAAGAATTAGATTACGTTGTCACAGAAAAAGATCGCATCGAA
GGGAAATTCACAATCATTCGCCGCGGTAAAAAGAAATATTTCTTAATTCGTTATTAA

Upstream 100 bases:

>100_bases
GGTGGTATCGCGATGATCAAAATCGTCCCTTATTTGAGGGGCGATTTTTTTATGTTTTCACATTTCACGCCACATTACAT
TAAAGGAGAGTATGTAGAAT

Downstream 100 bases:

>100_bases
GAAATAGAACATATAAGACAGAAAGGCTTTGAGGATTTATTCCTCAAAGCCTTTTTGTCTTAAACTTTTTCGTATGTTCA
TACAGAAGTCACACGCTAGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1 [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPILMLRRFQLAGHQPIALVGGGTGMIGDPS
GKKAERTLNTKDTVAYYTESIKKQLSNFLEFENVENPAVMANNYDWLGNLDVITFLRDIGKNFGLNYMLAKDTVASRLET
GISFTEFSYMILQSYDFLNLYKNHGCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAVW
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILDLEKQVAEAPEKRVAQKALASEMTKLVHGEEALEQAIKISA
ALFSGSVAELTADEIEQGFKDVPSVDRSAEDTGLIDLLVESKISPSKRQAREDVTNGAIYINGERTQELDYVVTEKDRIE
GKFTIIRRGKKKYFLIRY

Sequences:

>Translated_418_residues
MNILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPILMLRRFQLAGHQPIALVGGGTGMIGDPS
GKKAERTLNTKDTVAYYTESIKKQLSNFLEFENVENPAVMANNYDWLGNLDVITFLRDIGKNFGLNYMLAKDTVASRLET
GISFTEFSYMILQSYDFLNLYKNHGCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAVW
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILDLEKQVAEAPEKRVAQKALASEMTKLVHGEEALEQAIKISA
ALFSGSVAELTADEIEQGFKDVPSVDRSAEDTGLIDLLVESKISPSKRQAREDVTNGAIYINGERTQELDYVVTEKDRIE
GKFTIIRRGKKKYFLIRY
>Mature_418_residues
MNILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPILMLRRFQLAGHQPIALVGGGTGMIGDPS
GKKAERTLNTKDTVAYYTESIKKQLSNFLEFENVENPAVMANNYDWLGNLDVITFLRDIGKNFGLNYMLAKDTVASRLET
GISFTEFSYMILQSYDFLNLYKNHGCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAVW
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILDLEKQVAEAPEKRVAQKALASEMTKLVHGEEALEQAIKISA
ALFSGSVAELTADEIEQGFKDVPSVDRSAEDTGLIDLLVESKISPSKRQAREDVTNGAIYINGERTQELDYVVTEKDRIE
GKFTIIRRGKKKYFLIRY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=402, Percent_Identity=36.81592039801, Blast_Score=251, Evalue=9e-67,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=54.6318289786223, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25145802, Length=399, Percent_Identity=37.34335839599, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI25145805, Length=321, Percent_Identity=35.202492211838, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=35.3211009174312, Blast_Score=283, Evalue=4e-77,
Organism=Drosophila melanogaster, GI20130383, Length=422, Percent_Identity=41.2322274881517, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47113; Mature: 47113

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPILMLRRFQL
CCCHHHCHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHQPIALVGGGTGMIGDPSGKKAERTLNTKDTVAYYTESIKKQLSNFLEFENVENPAVM
CCCCCEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
ANNYDWLGNLDVITFLRDIGKNFGLNYMLAKDTVASRLETGISFTEFSYMILQSYDFLNL
ECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHCHHHHH
YKNHGCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAVW
HHCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCCCCCEEE
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILDLEKQVAEAPEKRVAQKALAS
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHH
EMTKLVHGEEALEQAIKISAALFSGSVAELTADEIEQGFKDVPSVDRSAEDTGLIDLLVE
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
SKISPSKRQAREDVTNGAIYINGERTQELDYVVTEKDRIEGKFTIIRRGKKKYFLIRY
CCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEECCCHHCCCEEEEEECCCCCEEEEEC
>Mature Secondary Structure
MNILQDLEFRGLINQQTDAEGLEQLLEKESVKLYCGFDPTADSLHIGHMLPILMLRRFQL
CCCHHHCHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHQPIALVGGGTGMIGDPSGKKAERTLNTKDTVAYYTESIKKQLSNFLEFENVENPAVM
CCCCCEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
ANNYDWLGNLDVITFLRDIGKNFGLNYMLAKDTVASRLETGISFTEFSYMILQSYDFLNL
ECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHCHHHHH
YKNHGCRLQIGGSDQWGNITAGLELIRKSEEDAKAFGLTIPLVTKSDGTKFGKTEGGAVW
HHCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCCCCCEEE
LDPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLSHEEILDLEKQVAEAPEKRVAQKALAS
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHH
EMTKLVHGEEALEQAIKISAALFSGSVAELTADEIEQGFKDVPSVDRSAEDTGLIDLLVE
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
SKISPSKRQAREDVTNGAIYINGERTQELDYVVTEKDRIEGKFTIIRRGKKKYFLIRY
CCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEECCCHHCCCEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]