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The map label for this gene is mdeA [H]

Identifier: 152977021

GI number: 152977021

Start: 3396596

End: 3397786

Strand: Reverse

Name: mdeA [H]

Synonym: Bcer98_3326

Alternate gene names: 152977021

Gene position: 3397786-3396596 (Counterclockwise)

Preceding gene: 152977026

Following gene: 152977019

Centisome position: 83.14

GC content: 37.78

Gene sequence:

>1191_bases
ATGAAAAAGAGCAATATGGAGACAGATTTCATTCATTATGGATATGAACCAGGTGTACATAAAGGAAGCTTAACGCCACC
TTTATTTCAAACTTCAACTTATACATTTGAAACAGCGAAACAAGGGGAAGATAGTTTTACTGGGCAAGATTCATCCTATA
TTTACTCACGGCTTGGTAATCCGACTGTAGAGTTATTTGAAGAACGCATGGCAGCTTTAGAAGGGGGAGAAGTTGCATTA
GCGTTTGGATCGGGTATGGCTTCTATTTCGGCGACATTGCTTGCTTGTTTACGTGCAGGGGATCATATGATTTGTTCCAA
TGGCTTATACGGTTGTACGTACGGTTTTTTGGAAGTGTTAGAGGAAAAATTTATGATTTCACATTCATTTTGTGATATGG
AAACGGATGAGCAGATTGAGAAAGAAATTCTTCCGAATACGAAGTTGATTTTCGTTGAAACACCTATTAATCCAACAATG
AAACTGATTGATTTAGAAAAAGTGATTAAAGTAGCAAAGAAAAATCATTTACTTGTTATTGTTGATAATACATTTTGTTC
TCCATACTTACAAAATCCACTTGTTCTTGGGTGTGATGCGGTTCTTCATAGCGCAACGAAATATATTGGGGGACATGGTG
ATGTAGTTGCGGGAGTTACTGTTTGTAAAACGAGGGAGTTAGCCGAAAAAATCCGTCCGATTCGAAAAGATATTGGGGGA
ATTATGGCGCCTTTTGATGCATGGTTATTATTAAGAGGGCTAAAAACGTTAGCTGTTCGAATGGATCGACATTGTGATAA
TGCGGAGAAGGTAGTAGCTTTTTTGAAAAATCATGAAAAGGTTGAAAAGGTTTGGTATCCAGAAGGAGAACGAGCATCTC
GGCAAATGAAGCGTGGTGGGGGTGTTATTTCGTTCTCTATTAAAGGAGGAAAAGAAGAAGCACAAGCTTTTATGGACAAA
TTAAATTTGATTGCGATTGCGGTCAGTCTTGGTGATACAGAAACATTAGTACAGCATCCCGCGACAATGACACATGCGGT
CATTCCAGCAGAAGTTCGTCAGAAAATGGAGATTTTTGATAATTTAATTCGTTTATCTGTCGGTTTAGAATCATGGGAAG
ATATTATTTCTGATTTACAGCAGGCGTTAAATAGCAGCAACGTTATATCCTCTAGCATACATTATAAATAA

Upstream 100 bases:

>100_bases
AAATTTGGAAATGACAAGGAGGGATTCAGAACATTCAGAAAGAATTTTAGAAACGTATAGAAAACGTTTACATAATATAG
AAACATAAGGGGGATGTTTT

Downstream 100 bases:

>100_bases
AAAGAATGAGCTATCGATTTAGTAATCATTACTTGATCGATAGCTCATTCTTTTCTTACTTACATATGTTTTAGGAGCGT
TTCCACAAATTTTTCTAAAT

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKKSNMETDFIHYGYEPGVHKGSLTPPLFQTSTYTFETAKQGEDSFTGQDSSYIYSRLGNPTVELFEERMAALEGGEVAL
AFGSGMASISATLLACLRAGDHMICSNGLYGCTYGFLEVLEEKFMISHSFCDMETDEQIEKEILPNTKLIFVETPINPTM
KLIDLEKVIKVAKKNHLLVIVDNTFCSPYLQNPLVLGCDAVLHSATKYIGGHGDVVAGVTVCKTRELAEKIRPIRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKVVAFLKNHEKVEKVWYPEGERASRQMKRGGGVISFSIKGGKEEAQAFMDK
LNLIAIAVSLGDTETLVQHPATMTHAVIPAEVRQKMEIFDNLIRLSVGLESWEDIISDLQQALNSSNVISSSIHYK

Sequences:

>Translated_396_residues
MKKSNMETDFIHYGYEPGVHKGSLTPPLFQTSTYTFETAKQGEDSFTGQDSSYIYSRLGNPTVELFEERMAALEGGEVAL
AFGSGMASISATLLACLRAGDHMICSNGLYGCTYGFLEVLEEKFMISHSFCDMETDEQIEKEILPNTKLIFVETPINPTM
KLIDLEKVIKVAKKNHLLVIVDNTFCSPYLQNPLVLGCDAVLHSATKYIGGHGDVVAGVTVCKTRELAEKIRPIRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKVVAFLKNHEKVEKVWYPEGERASRQMKRGGGVISFSIKGGKEEAQAFMDK
LNLIAIAVSLGDTETLVQHPATMTHAVIPAEVRQKMEIFDNLIRLSVGLESWEDIISDLQQALNSSNVISSSIHYK
>Mature_396_residues
MKKSNMETDFIHYGYEPGVHKGSLTPPLFQTSTYTFETAKQGEDSFTGQDSSYIYSRLGNPTVELFEERMAALEGGEVAL
AFGSGMASISATLLACLRAGDHMICSNGLYGCTYGFLEVLEEKFMISHSFCDMETDEQIEKEILPNTKLIFVETPINPTM
KLIDLEKVIKVAKKNHLLVIVDNTFCSPYLQNPLVLGCDAVLHSATKYIGGHGDVVAGVTVCKTRELAEKIRPIRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKVVAFLKNHEKVEKVWYPEGERASRQMKRGGGVISFSIKGGKEEAQAFMDK
LNLIAIAVSLGDTETLVQHPATMTHAVIPAEVRQKMEIFDNLIRLSVGLESWEDIISDLQQALNSSNVISSSIHYK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=396, Percent_Identity=40.1515151515151, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI299473759, Length=396, Percent_Identity=36.6161616161616, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI25453487, Length=395, Percent_Identity=34.9367088607595, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=36.096256684492, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789383, Length=390, Percent_Identity=27.1794871794872, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560056, Length=386, Percent_Identity=39.119170984456, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71980708, Length=367, Percent_Identity=40.8719346049046, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17538055, Length=386, Percent_Identity=39.6373056994819, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI193202265, Length=88, Percent_Identity=39.7727272727273, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=40.6649616368286, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6323333, Length=444, Percent_Identity=32.2072072072072, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=420, Percent_Identity=30.4761904761905, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=343, Percent_Identity=29.4460641399417, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=29.3670886075949, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI19922554, Length=392, Percent_Identity=36.734693877551, Blast_Score=255, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43890; Mature: 43890

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSNMETDFIHYGYEPGVHKGSLTPPLFQTSTYTFETAKQGEDSFTGQDSSYIYSRLGN
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECHHCCCCCCCCCCHHHHHHHCCC
PTVELFEERMAALEGGEVALAFGSGMASISATLLACLRAGDHMICSNGLYGCTYGFLEVL
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
EEKFMISHSFCDMETDEQIEKEILPNTKLIFVETPINPTMKLIDLEKVIKVAKKNHLLVI
HHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
VDNTFCSPYLQNPLVLGCDAVLHSATKYIGGHGDVVAGVTVCKTRELAEKIRPIRKDIGG
EECCCCCHHHCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKVVAFLKNHEKVEKVWYPEGERASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCC
GVISFSIKGGKEEAQAFMDKLNLIAIAVSLGDTETLVQHPATMTHAVIPAEVRQKMEIFD
CEEEEEECCCHHHHHHHHHHHHEEEEEEECCCHHHHHHCCCHHHHHCCCHHHHHHHHHHH
NLIRLSVGLESWEDIISDLQQALNSSNVISSSIHYK
HHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKSNMETDFIHYGYEPGVHKGSLTPPLFQTSTYTFETAKQGEDSFTGQDSSYIYSRLGN
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECHHCCCCCCCCCCHHHHHHHCCC
PTVELFEERMAALEGGEVALAFGSGMASISATLLACLRAGDHMICSNGLYGCTYGFLEVL
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
EEKFMISHSFCDMETDEQIEKEILPNTKLIFVETPINPTMKLIDLEKVIKVAKKNHLLVI
HHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
VDNTFCSPYLQNPLVLGCDAVLHSATKYIGGHGDVVAGVTVCKTRELAEKIRPIRKDIGG
EECCCCCHHHCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKVVAFLKNHEKVEKVWYPEGERASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCC
GVISFSIKGGKEEAQAFMDKLNLIAIAVSLGDTETLVQHPATMTHAVIPAEVRQKMEIFD
CEEEEEECCCHHHHHHHHHHHHEEEEEEECCCHHHHHHCCCHHHHHCCCHHHHHHHHHHH
NLIRLSVGLESWEDIISDLQQALNSSNVISSSIHYK
HHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]