Click here to switch to the map view.

The map label for this gene is ackA [H]

Identifier: 152977005

GI number: 152977005

Start: 3379719

End: 3380912

Strand: Reverse

Name: ackA [H]

Synonym: Bcer98_3310

Alternate gene names: 152977005

Gene position: 3380912-3379719 (Counterclockwise)

Preceding gene: 152977006

Following gene: 152977004

Centisome position: 82.72

GC content: 38.94

Gene sequence:

>1194_bases
ATGTCAAAAATCATCGCGATTAATGCAGGAAGTTCTTCCTTGAAGTTTCAATTATTTGAAATGCCAAGTGAGAAAGTATT
AACAAAAGGTTTAGTAGAACGTATTGGTTTAGAAGACAGTATTTTCACAATTACTGTAAATGGTGAGAAGCAAAAAGAAA
TTACAAATATTCCAGATCATGCAGTGGCAGTTAATATGCTTCTTAATAAATTAACTGAAAATGGAATCGTGAAATCTCTT
GATGAGATTAGCGGTATCGGTCACCGTGTTGTACATGGCGGTGAAAAATTTGCTGACTCTGTATTAATTACAGATCAAGT
ATTAGCAGATATTGAAGACTTAAGCGAATTAGCACCACTTCATAATCCAGCAAACATTGTTGGTATTAAAGCGTTCCAAG
AAGTACTTCCAAACGTACCAGCAGTAGCAGTATTCGATACAGCATTCCACCAAACAATGCCAGAATCTGCGTATCTTTAC
AGCTTACCATATGAGTACTATGAGAAATACGGTATCCGTAAATATGGTTTCCATGGAACTTCTCATAAATATGTAACAGA
GCGTGCAGCTGAATTATTAGGCCGTCCACTTGAAAGCTTACGTCTTCTTTCTTGTCATTTAGGTAATGGTGCAAGTATCG
CAGCTGTAGAAGGTGGAAAATCTATCGATACGTCAATGGGATTCACTCCACTTGCTGGTGTAACAATGGGGACGCGTTCT
GGTAACATTGACCCTGCGCTAATTCCATACATCATGGAAAAAACGGGACAAAGTGTAGAAGAAGTAGTAAATGTATTAAA
CAAGAAAAGTGGTATGTTAGGTCTTACTGGATATTCTAGTGACTTACGTGACATTATTGCGAAAGAAGAAGAAGGCGACC
ACCGTGCAAAAGTAGCACTTGATGTGTTCGTAAGCCGTATTCACAAATACATTGGTTCTTACGCAGCTCGCATGAAAGGT
GTTGACGCAATCATCTTCACAGCTGGTATAGGTGAAAATAGCGCCATCATTCGTGAGCGCGTATTAGAAGGTCTTGAGTA
CATGGGCGTATACTTTGATGCAAAACGCAACAACGTATTCGGTGAAGAAGCATTTATCAGCTTCCCACACTCTCCAGTAA
AAATTATCGTAATTCCAACTGACGAAGAAGTTATGATCGCTCGTGACGTTGTTCGTCTTGGAAATGTTGGTTAA

Upstream 100 bases:

>100_bases
CAAAATGAAACGTTTTCATTTTGATGAATTTAAAGAGATAAAATCGAGCGGTTTGTGGTTTTCCACATAACTACCAAGAG
AAAAAGGGGCGAAAGATTAG

Downstream 100 bases:

>100_bases
TATATATCAGAAAGACGGAATCTTACATGATTCCGTCTTTTTTTGTGAGGAAATTGAAAATCTGTCTCTATGTATGAAAT
TCACATATGTGTACTTACCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSKIIAINAGSSSLKFQLFEMPSEKVLTKGLVERIGLEDSIFTITVNGEKQKEITNIPDHAVAVNMLLNKLTENGIVKSL
DEISGIGHRVVHGGEKFADSVLITDQVLADIEDLSELAPLHNPANIVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAYLY
SLPYEYYEKYGIRKYGFHGTSHKYVTERAAELLGRPLESLRLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMEKTGQSVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYAARMKG
VDAIIFTAGIGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFISFPHSPVKIIVIPTDEEVMIARDVVRLGNVG

Sequences:

>Translated_397_residues
MSKIIAINAGSSSLKFQLFEMPSEKVLTKGLVERIGLEDSIFTITVNGEKQKEITNIPDHAVAVNMLLNKLTENGIVKSL
DEISGIGHRVVHGGEKFADSVLITDQVLADIEDLSELAPLHNPANIVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAYLY
SLPYEYYEKYGIRKYGFHGTSHKYVTERAAELLGRPLESLRLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMEKTGQSVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYAARMKG
VDAIIFTAGIGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFISFPHSPVKIIVIPTDEEVMIARDVVRLGNVG
>Mature_396_residues
SKIIAINAGSSSLKFQLFEMPSEKVLTKGLVERIGLEDSIFTITVNGEKQKEITNIPDHAVAVNMLLNKLTENGIVKSLD
EISGIGHRVVHGGEKFADSVLITDQVLADIEDLSELAPLHNPANIVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAYLYS
LPYEYYEKYGIRKYGFHGTSHKYVTERAAELLGRPLESLRLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRSG
NIDPALIPYIMEKTGQSVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYAARMKGV
DAIIFTAGIGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFISFPHSPVKIIVIPTDEEVMIARDVVRLGNVG

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=45.1127819548872, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI145693184, Length=397, Percent_Identity=44.8362720403023, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43303; Mature: 43172

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIAINAGSSSLKFQLFEMPSEKVLTKGLVERIGLEDSIFTITVNGEKQKEITNIPDH
CCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCHH
AVAVNMLLNKLTENGIVKSLDEISGIGHRVVHGGEKFADSVLITDQVLADIEDLSELAPL
HHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHEHHHHHHHHHHHHHHHHCCC
HNPANIVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAYLYSLPYEYYEKYGIRKYGFHGT
CCCCCEEHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLESLRLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMEKTGQSVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVAL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHH
DVFVSRIHKYIGSYAARMKGVDAIIFTAGIGENSAIIRERVLEGLEYMGVYFDAKRNNVF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
GEEAFISFPHSPVKIIVIPTDEEVMIARDVVRLGNVG
CCHHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKIIAINAGSSSLKFQLFEMPSEKVLTKGLVERIGLEDSIFTITVNGEKQKEITNIPDH
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCHH
AVAVNMLLNKLTENGIVKSLDEISGIGHRVVHGGEKFADSVLITDQVLADIEDLSELAPL
HHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHEHHHHHHHHHHHHHHHHCCC
HNPANIVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAYLYSLPYEYYEKYGIRKYGFHGT
CCCCCEEHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLESLRLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMEKTGQSVEEVVNVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVAL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHH
DVFVSRIHKYIGSYAARMKGVDAIIFTAGIGENSAIIRERVLEGLEYMGVYFDAKRNNVF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
GEEAFISFPHSPVKIIVIPTDEEVMIARDVVRLGNVG
CCHHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]