Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 152976973

GI number: 152976973

Start: 3344803

End: 3345741

Strand: Reverse

Name: mdh [H]

Synonym: Bcer98_3276

Alternate gene names: 152976973

Gene position: 3345741-3344803 (Counterclockwise)

Preceding gene: 152976974

Following gene: 152976972

Centisome position: 81.86

GC content: 39.83

Gene sequence:

>939_bases
ATGACAATCAAACGCAAAAAAGTATCAGTCATCGGTGCGGGATTTACAGGAGCAACAACGGCGTTCTTATTAGCTCAAAA
AGAGCTTGCAGATGTTGTACTAGTGGATATTCCACAGCTTGAAAATCCAACAAAAGGGAAAGCGTTAGATATGTTAGAGG
CAAGCCCTGTCCAAGGTTTTGATGCTAACATTATCGGTACATCTGATTACGCAGATACTGCTGATTCTGACGTTGTAATT
ATTACAGCAGGAATCGCGCGTAAACCGGGTATGAGCCGCGATGATTTAGTAGCGACGAATTCTAAAATTATGAAAAGTGT
TACAAAAGAAATTGCAAAACACTCACCAGATACAATTATTATTGTGTTAACAAATCCAGTTGATGCAATGACATATTCTG
TATTTAAAGAAGCAGGATTTCCAAAAGAACGCGTTATTGGTCAATCTGGTGTATTAGATACAGCTCGTTTCCGTACATTT
ATCGCACAAGAATTGAACCTTTCTGTTAAAGATATTACTGGATTTGTTCTTGGGGGTCACGGTGATGATATGGTACCGCT
TGTTCGCTATTCGTATGCAGGTGGTATACCGCTAGAAACACTCATTTCGAAAGAACGTTTAGATGCAATTGTGGAGCGTA
CACGTAAAGGTGGCGGTGAAATTGTAAATCTATTAGGAAATGGTAGTGCATATTATGCACCGGCTGCTTCTCTCGTTGAG
ATGACGGAAGCAATCCTAAAAGACCAGCGCCGTGTGTTACCAGCTATTGCATATCTTGAAGGGGAATATGGTTATCGTGA
TCTTTACCTAGGTGTACCAGTAATTTTAGGTGGTAACGGTATTGAAAAAGTGATTGAGTTAGAGCTTCGCGAAGAAGAAA
AAATGGCGTTAGATCGTTCAGTTGAATCTGTGCGTAACGTTATGGAAATTCTTTCTTAA

Upstream 100 bases:

>100_bases
GGTGCAACTGAAGTGAAATGTTCTGAATTTGCTGATGCTCTTATTAACAATATGGACGTAGCAGTAATTAAAAACGCATA
ATAAAAGCGGGGGTGAACGT

Downstream 100 bases:

>100_bases
GTTAAATATGTGCTAGGAAAAAGATTCGGGTTCCGAATCTTTTTCCGCTATTTAGTAGATTGTTTTTTTGAGATAATTTG
AAAACGTTATCAATCTTCAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANIIGTSDYADTADSDVVI
ITAGIARKPGMSRDDLVATNSKIMKSVTKEIAKHSPDTIIIVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTF
IAQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLISKERLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVE
MTEAILKDQRRVLPAIAYLEGEYGYRDLYLGVPVILGGNGIEKVIELELREEEKMALDRSVESVRNVMEILS

Sequences:

>Translated_312_residues
MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANIIGTSDYADTADSDVVI
ITAGIARKPGMSRDDLVATNSKIMKSVTKEIAKHSPDTIIIVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTF
IAQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLISKERLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVE
MTEAILKDQRRVLPAIAYLEGEYGYRDLYLGVPVILGGNGIEKVIELELREEEKMALDRSVESVRNVMEILS
>Mature_311_residues
TIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANIIGTSDYADTADSDVVII
TAGIARKPGMSRDDLVATNSKIMKSVTKEIAKHSPDTIIIVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFI
AQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLISKERLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVEM
TEAILKDQRRVLPAIAYLEGEYGYRDLYLGVPVILGGNGIEKVIELELREEEKMALDRSVESVRNVMEILS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=318, Percent_Identity=37.7358490566038, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI221136809, Length=318, Percent_Identity=37.7358490566038, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=35.5345911949685, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI5031857, Length=317, Percent_Identity=34.7003154574132, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=34.8101265822785, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=34.9358974358974, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=34.9358974358974, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI291575128, Length=319, Percent_Identity=32.6018808777429, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI4557032, Length=319, Percent_Identity=32.6018808777429, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI260099727, Length=198, Percent_Identity=38.3838383838384, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI260099725, Length=198, Percent_Identity=38.3838383838384, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=33.6683417085427, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI21735621, Length=309, Percent_Identity=30.7443365695793, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI103472011, Length=312, Percent_Identity=26.9230769230769, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789632, Length=293, Percent_Identity=32.0819112627986, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=30.8724832214765, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17554310, Length=307, Percent_Identity=31.2703583061889, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322765, Length=296, Percent_Identity=30.7432432432432, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=284, Percent_Identity=28.8732394366197, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17136226, Length=313, Percent_Identity=31.629392971246, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=31.4569536423841, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24647881, Length=315, Percent_Identity=29.8412698412698, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24663599, Length=311, Percent_Identity=27.9742765273312, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663595, Length=242, Percent_Identity=28.5123966942149, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33737; Mature: 33606

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANIIGTSDYADTADSDVVIITAGIARKPGMSRDDLVATNSKIMKSVTKEIAKHSPDTII
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEE
IVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFIAQELNLSVKDITGFVLGGH
EEEECCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCC
GDDMVPLVRYSYAGGIPLETLISKERLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVE
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MTEAILKDQRRVLPAIAYLEGEYGYRDLYLGVPVILGGNGIEKVIELELREEEKMALDRS
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHH
VESVRNVMEILS
HHHHHHHHHHHC
>Mature Secondary Structure 
TIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANIIGTSDYADTADSDVVIITAGIARKPGMSRDDLVATNSKIMKSVTKEIAKHSPDTII
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEE
IVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFIAQELNLSVKDITGFVLGGH
EEEECCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCC
GDDMVPLVRYSYAGGIPLETLISKERLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVE
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MTEAILKDQRRVLPAIAYLEGEYGYRDLYLGVPVILGGNGIEKVIELELREEEKMALDRS
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHH
VESVRNVMEILS
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA