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The map label for this gene is dnaG [H]

Identifier: 152976725

GI number: 152976725

Start: 3068351

End: 3070147

Strand: Reverse

Name: dnaG [H]

Synonym: Bcer98_3020

Alternate gene names: 152976725

Gene position: 3070147-3068351 (Counterclockwise)

Preceding gene: 152976726

Following gene: 152976724

Centisome position: 75.12

GC content: 37.62

Gene sequence:

>1797_bases
ATGGGGAACAGAATTCCCGAAGAAGTTGTTGAACAGATTCGGACATCATCTGATATTGTAGAAGTAATTGGTGAGTATGT
TCAGCTAAGAAAGCAAGGGCGTAATTATTTTGGACTTTGTCCATTTCATGGTGAGAATTCTCCCTCCTTTTCTGTTTCAT
CAGATAAGCAAATTTTCCATTGCTTTGGATGTGGGGAAGGTGGAAATGTTTTTTCCTTTCTCATGAAGATGGAAGGCCTT
TCTTTCACAGAAGCTGTACAGAAACTTGGAGAAAGAAATCATATAGCAGTCGCAGATTATACATCAGGAAAAGAACAGCA
AGACAATATATCTGATGACAATGTCATTATGTTACAGGCTCACGAACTCTTAAAAAAGTATTATCATCATTTGTTGGTAA
ATACAGAAGAGGGGAATGAAGCTCTTTCTTATTTATTAAATCGTGGTATTACGAAGGAAATGATTGAGAAGTTTGAAATT
GGTTATGCATCACCGGATTGGGATGCAGCGGCGAAAATATTACAAAAAAGAGGTTTTTCGCTAATGACCATGGAGCAAGC
GGGCCTCCTTGTAAGAAGTGAAAAGGATGGTAGTCATTACGACCGATTCCGTGGTAGAGTAATGTTTCCGATTCATACAC
TACAAGGAAAAGTGGCAGCATTTAGTGGAAGGGCGCTTGGGGATGATACTCCTAAATATTTAAATAGCCCAGAAACACCT
ATCTTTCATAAGAGCAAGTTGCTGTACAATTTCCATCAGGCAAGGCCGTTTATTAGAAAGCGTGGGCAGGTCGTCCTTTT
TGAAGGTTATGCTGATGTTCTAGCTGCTGTAAAAAGCGGGGTTGAAGAAGCTGTTGCAACAATGGGAACAGCTTTAACGG
AAGAACAGGCGAAACTTCTGCGACGTAATGTTGAAACTGTTGTTCTTTGCTATGATGGTGATAAGGCTGGACAAGAAGCG
ACAATGAAAGCGGGGCAACTCTTAACTCAAGTTGGTTGTCAAGTTAAAGTTACTGTATTGCCAGAGAAATTAGATCCTGA
TGAATATGTGCAACAATATGGAACAAAGGCTTTTGAAAATCTCGTTAAATCAAGTATAAGTTTTGTTGGATTTAAAATAA
ATTATTTGCGTTTAGGAAAAAATTTGCAAGATGAGTCTGGTAAAGAAGAATATGTGAAAAGTGTTTTAAAAGAATTATCA
TTATTGCAAGATGCGATGCAGGCAGAGTCCTATTTAAAATCGTTATCACAAGAATTTGCATATTCCATGGAAACACTTTT
AAGTCAATTGCACCAATATCGCAAAGAACAAAAGATACAGCAAAAACAAGCGAAGCAAATTTCGAAGCCATCTCAAATTG
TTCAAACGAAGCCGAAACGATCCGGCTTCGAAAGGGCAGAGAGGGAACTGATTTACCATATGTTGCAAAGTGCCGAAGTA
GCAGTGCGTATAGAGCCTCATATAGAAGATTTTCATACGGAAGAACATAAAGGGATTTTATATGAACTATATGCATATTA
TGAAAAGGGAAATGAACCTTCAGTCGGAAATTTTTTAAACTGGCTATCTGATGAAAAGTTGAAAAATATCGTTACCAATA
TTTCGACAGATGAGTTTATCAATCCAGAATATACAGATGAAATACTGGAAGGACACTTGGAAGCGTTAAAGCGCCATAAG
GAAAAGTTGAAAAAGATGGAAACCATCTTTACAGTAAAGCAAATGGAAAAAGCTGATCCGCTAGAAGCTGCTAAATTTTA
CGCAGCGTATTTGCAAAATCAAAAAGCAAGAAGATAG

Upstream 100 bases:

>100_bases
ATCGACAGTCTTTTTTAACAAGTTTAGAAAAAATATTGTCGAATTATAAAGGAATCCTTTAATTTGTATCGAATACAAAA
TACGACACGGAGATGGAGTT

Downstream 100 bases:

>100_bases
CTTTTTGCATAAAATTTTAGTATAATGAATGTTTGTATCTCGCGTAAGGAGGGGAACAAATGGCTGATAAACCAGCTCGT
TCTAAACAAATTGAAACTGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MGNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFHCFGCGEGGNVFSFLMKMEGL
SFTEAVQKLGERNHIAVADYTSGKEQQDNISDDNVIMLQAHELLKKYYHHLLVNTEEGNEALSYLLNRGITKEMIEKFEI
GYASPDWDAAAKILQKRGFSLMTMEQAGLLVRSEKDGSHYDRFRGRVMFPIHTLQGKVAAFSGRALGDDTPKYLNSPETP
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLLRRNVETVVLCYDGDKAGQEA
TMKAGQLLTQVGCQVKVTVLPEKLDPDEYVQQYGTKAFENLVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELS
LLQDAMQAESYLKSLSQEFAYSMETLLSQLHQYRKEQKIQQKQAKQISKPSQIVQTKPKRSGFERAERELIYHMLQSAEV
AVRIEPHIEDFHTEEHKGILYELYAYYEKGNEPSVGNFLNWLSDEKLKNIVTNISTDEFINPEYTDEILEGHLEALKRHK
EKLKKMETIFTVKQMEKADPLEAAKFYAAYLQNQKARR

Sequences:

>Translated_598_residues
MGNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFHCFGCGEGGNVFSFLMKMEGL
SFTEAVQKLGERNHIAVADYTSGKEQQDNISDDNVIMLQAHELLKKYYHHLLVNTEEGNEALSYLLNRGITKEMIEKFEI
GYASPDWDAAAKILQKRGFSLMTMEQAGLLVRSEKDGSHYDRFRGRVMFPIHTLQGKVAAFSGRALGDDTPKYLNSPETP
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLLRRNVETVVLCYDGDKAGQEA
TMKAGQLLTQVGCQVKVTVLPEKLDPDEYVQQYGTKAFENLVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELS
LLQDAMQAESYLKSLSQEFAYSMETLLSQLHQYRKEQKIQQKQAKQISKPSQIVQTKPKRSGFERAERELIYHMLQSAEV
AVRIEPHIEDFHTEEHKGILYELYAYYEKGNEPSVGNFLNWLSDEKLKNIVTNISTDEFINPEYTDEILEGHLEALKRHK
EKLKKMETIFTVKQMEKADPLEAAKFYAAYLQNQKARR
>Mature_597_residues
GNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFHCFGCGEGGNVFSFLMKMEGLS
FTEAVQKLGERNHIAVADYTSGKEQQDNISDDNVIMLQAHELLKKYYHHLLVNTEEGNEALSYLLNRGITKEMIEKFEIG
YASPDWDAAAKILQKRGFSLMTMEQAGLLVRSEKDGSHYDRFRGRVMFPIHTLQGKVAAFSGRALGDDTPKYLNSPETPI
FHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLLRRNVETVVLCYDGDKAGQEAT
MKAGQLLTQVGCQVKVTVLPEKLDPDEYVQQYGTKAFENLVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELSL
LQDAMQAESYLKSLSQEFAYSMETLLSQLHQYRKEQKIQQKQAKQISKPSQIVQTKPKRSGFERAERELIYHMLQSAEVA
VRIEPHIEDFHTEEHKGILYELYAYYEKGNEPSVGNFLNWLSDEKLKNIVTNISTDEFINPEYTDEILEGHLEALKRHKE
KLKKMETIFTVKQMEKADPLEAAKFYAAYLQNQKARR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=492, Percent_Identity=34.5528455284553, Blast_Score=282, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68225; Mature: 68094

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFH
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGEGGNVFSFLMKMEGLSFTEAVQKLGERNHIAVADYTSGKEQQDNISDDNVIMLQA
EEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEHH
HELLKKYYHHLLVNTEEGNEALSYLLNRGITKEMIEKFEIGYASPDWDAAAKILQKRGFS
HHHHHHHHHHHEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LMTMEQAGLLVRSEKDGSHYDRFRGRVMFPIHTLQGKVAAFSGRALGDDTPKYLNSPETP
EEEEHHCCCEEEECCCCCHHHHHCCCEEEEEHHHCCCHHHCCCCCCCCCCHHHCCCCCCC
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLL
CEEHHHHHHHHHHHCHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRNVETVVLCYDGDKAGQEATMKAGQLLTQVGCQVKVTVLPEKLDPDEYVQQYGTKAFEN
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELSLLQDAMQAESYLKSLSQEFA
HHHHHHHHHHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSMETLLSQLHQYRKEQKIQQKQAKQISKPSQIVQTKPKRSGFERAERELIYHMLQSAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCE
AVRIEPHIEDFHTEEHKGILYELYAYYEKGNEPSVGNFLNWLSDEKLKNIVTNISTDEFI
EEEECCCHHHHCCHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
NPEYTDEILEGHLEALKRHKEKLKKMETIFTVKQMEKADPLEAAKFYAAYLQNQKARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFH
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGEGGNVFSFLMKMEGLSFTEAVQKLGERNHIAVADYTSGKEQQDNISDDNVIMLQA
EEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEHH
HELLKKYYHHLLVNTEEGNEALSYLLNRGITKEMIEKFEIGYASPDWDAAAKILQKRGFS
HHHHHHHHHHHEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LMTMEQAGLLVRSEKDGSHYDRFRGRVMFPIHTLQGKVAAFSGRALGDDTPKYLNSPETP
EEEEHHCCCEEEECCCCCHHHHHCCCEEEEEHHHCCCHHHCCCCCCCCCCHHHCCCCCCC
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLL
CEEHHHHHHHHHHHCHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRNVETVVLCYDGDKAGQEATMKAGQLLTQVGCQVKVTVLPEKLDPDEYVQQYGTKAFEN
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELSLLQDAMQAESYLKSLSQEFA
HHHHHHHHHHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSMETLLSQLHQYRKEQKIQQKQAKQISKPSQIVQTKPKRSGFERAERELIYHMLQSAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCE
AVRIEPHIEDFHTEEHKGILYELYAYYEKGNEPSVGNFLNWLSDEKLKNIVTNISTDEFI
EEEECCCHHHHCCHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
NPEYTDEILEGHLEALKRHKEKLKKMETIFTVKQMEKADPLEAAKFYAAYLQNQKARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3919021; 3086839; 8969508; 9384377 [H]