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The map label for this gene is ldh [H]

Identifier: 152976570

GI number: 152976570

Start: 2932018

End: 2933118

Strand: Reverse

Name: ldh [H]

Synonym: Bcer98_2859

Alternate gene names: 152976570

Gene position: 2933118-2932018 (Counterclockwise)

Preceding gene: 152976571

Following gene: 152976569

Centisome position: 71.77

GC content: 38.33

Gene sequence:

>1101_bases
ATGGCATTAGAAATATTCGAATACTTAGAAAAATACGATTATGAGCAAGTAGTATTTTGTCAAGATAAAGAATCTGGATT
AAAAGCAATTATTGCAATTCATGATACAACACTTGGACCAGCTCTTGGTGGAACAAGAATGTGGACATATGATTCTGAAG
AAGCGGCGATTGAAGATGCACTGCGTCTTGCAAAAGGGATGACATATAAAAATGCAGCAGCTGGTTTAAATCTTGGTGGT
GCAAAAACAGTTATTATCGGCGACCCAAGAAAAGATAAAAGCGAAGCGATGTTCCGAGCGCTTGGACGTTACATTCAAGG
GTTAAACGGACGCTATATTACAGCAGAAGATGTTGGTACAACAGTAGATGATATGGATATTATCCATGAAGAAACAGATT
TTGTAACAGGTATTTCACCTTCATTCGGTTCTTCTGGAAATCCATCACCGGTAACTGCATATGGGGTATACCGTGGTATG
AAGGCAGCTGCGAAAGAAGCTTTTGGAACAGATAGCTTAGAAGGTAAAGTGATTGCTGTTCAAGGTGTTGGAAATGTGGC
GTATCACCTTTGCAAACATTTACATGCTGAATCAGCACAATTAATTGTTACAGATATTAATAAAGAAGCGGTACAACGCG
CAGTAGAAGAATTTGGAGCAACAGCAGTAGAACCAAACGAAATTTATGGTGTTGAATGTGATATTTATGCACCATGTGCG
CTAGGTGCAACAATTAATGATGAAACAATCCCACAATTTAAAGCAAAGGTAATCGCAGGTTCTGCAAATAACCAATTAAA
AGAAGACCGTCATGGTGATATCATTCATGAAATGGGAATTGTATACGCACCAGATTATGTTATTAATGCAGGCGGCGTAA
TTAACGTAGCTGATGAATTATATGGATACAATAGAGAACGTGCATTAAAACGTGTAGAGTCTATTTATGACACAATCGAA
AAAGTGATTGAAATTTCAAAGCGTGATTCTGTTCCAACTTACGTGGCAGCAGATCGTCTAGCTGAAGAGCGTATTGCAAG
CTTAAAAAATACTCGTAGCACATATTTACGCAACGGTCACGATATTATTAGCCGTCGCTAA

Upstream 100 bases:

>100_bases
GTGCTGATTCAGCAGAAACAAAAGTATATTCGTTAGCTTTGGCAATTGCCACTGCTTCTAAATAAACCAATAAGGGGAAA
ACAATTAGGGGGAAATGACA

Downstream 100 bases:

>100_bases
TCTTTCATATACAACTTCTACAGAAGCTGTGGTTACCTCTCATGAGGTTTCCACTGTCTTTTGAATTTATAGGTGGGGGT
AGCAACATTGTCGGTAAATC

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDALRLAKGMTYKNAAAGLNLGG
AKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGTTVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGM
KAAAKEAFGTDSLEGKVIAVQGVGNVAYHLCKHLHAESAQLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
LGATINDETIPQFKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERALKRVESIYDTIE
KVIEISKRDSVPTYVAADRLAEERIASLKNTRSTYLRNGHDIISRR

Sequences:

>Translated_366_residues
MALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDALRLAKGMTYKNAAAGLNLGG
AKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGTTVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGM
KAAAKEAFGTDSLEGKVIAVQGVGNVAYHLCKHLHAESAQLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
LGATINDETIPQFKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERALKRVESIYDTIE
KVIEISKRDSVPTYVAADRLAEERIASLKNTRSTYLRNGHDIISRR
>Mature_365_residues
ALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDALRLAKGMTYKNAAAGLNLGGA
KTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGTTVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGMK
AAAKEAFGTDSLEGKVIAVQGVGNVAYHLCKHLHAESAQLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCAL
GATINDETIPQFKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERALKRVESIYDTIEK
VIEISKRDSVPTYVAADRLAEERIASLKNTRSTYLRNGHDIISRR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=304, Percent_Identity=26.6447368421053, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4885281, Length=304, Percent_Identity=25.9868421052632, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24649283, Length=296, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 40017; Mature: 39885

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDA
CCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHH
LRLAKGMTYKNAAAGLNLGGAKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGT
HHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHCCCC
TVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGMKAAAKEAFGTDSLEGKVIAV
CHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QGVGNVAYHLCKHLHAESAQLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
ECCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECEECCEE
LGATINDETIPQFKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADEL
CCCCCCCCCCHHHHHEEEECCCCCCHHHCCCCCHHHHCCCEECCCEEEECCCEEEEHHHH
YGYNRERALKRVESIYDTIEKVIEISKRDSVPTYVAADRLAEERIASLKNTRSTYLRNGH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DIISRR
HHHCCC
>Mature Secondary Structure 
ALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDA
CHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHH
LRLAKGMTYKNAAAGLNLGGAKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGT
HHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHCCCC
TVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGMKAAAKEAFGTDSLEGKVIAV
CHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QGVGNVAYHLCKHLHAESAQLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
ECCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECEECCEE
LGATINDETIPQFKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADEL
CCCCCCCCCCHHHHHEEEECCCCCCHHHCCCCCHHHHCCCEECCCEEEECCCEEEEHHHH
YGYNRERALKRVESIYDTIEKVIEISKRDSVPTYVAADRLAEERIASLKNTRSTYLRNGH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DIISRR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9188201 [H]