| Definition | Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome. |
|---|---|
| Accession | NC_009674 |
| Length | 4,087,024 |
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The map label for this gene is ytbE [H]
Identifier: 152976507
GI number: 152976507
Start: 2873786
End: 2874613
Strand: Reverse
Name: ytbE [H]
Synonym: Bcer98_2796
Alternate gene names: 152976507
Gene position: 2874613-2873786 (Counterclockwise)
Preceding gene: 152976508
Following gene: 152976504
Centisome position: 70.34
GC content: 37.2
Gene sequence:
>828_bases ATGTATATTCCAACAACGACACTTCATAACGGTGTGAAAATGCCAATGCTTGGTTTAGGGGTATATAAAGCCAAAGAGGG AGAAGAAGTAAAACGAGCGGTAAAAACGGCTTTAGAAATTGGGTATCGTTCTATTGATACAGCGGCAGTATATGAAAATG AAAGCGGTGTCGGTGAAGCGGTTCGTAACTCGGGTATTCCGCGTGAAGAGTTATTTATTACAACAAAAGTATGGAATGAT TATCAAGGATATGAAGAGACGCTTCAAGCGTTTGAAAAAAGTTTGGAAAAGTTACAAATGGATTATGTAGATTTATATTT AATTCACTGGCCAGTTAGAGGAAAGTATATCGATACATACCGAGCTCTAGAGAAGTTATATAAAGAAGGGAAAGTGCGAG CGATTGGAGTTTCTAATTTCCATCAACATCATTTAGAACGTCTTTTAGCCAACTGTCATGTGAAGCCGATGGTAAACCAA GTGGAGTTGCATCCAATGTTTGCGCAGTTTGAGTTGCGTGACTTTTGTCAAAACAAACAAATTCAAATGGAAGCGTGGAG CCCGTTAATGAGGGGGAGAGAAGTATTTGAGCATCCGATTATTCAAGAGATTGCTAGTAAATATAACAAAACACCTGCCC AAATTATTTTACGCTGGGATATTCAAAGTGGCATTGTAACCATTCCAAAGTCCGTTACACCATCTCGTATTAAAGAGAAC TTTACGATCTTTGATTTTTCATTAACAGAAGATGAAATGAAACAAATTGATACATTAGATTGTAATGTACGTATTGGGAC AAATCCTGATAAGTACGATACAATGTAA
Upstream 100 bases:
>100_bases TTTTTTCGTAAATCTTGTTCAAATGAAGTGAAGGTTATAAAAATGATAGAACTTGTTGATTAAGTTTTTATATTGCAGGG ATAAAGAAGGAGGAGCAATC
Downstream 100 bases:
>100_bases AAAAAAGCTACCATTTGGTAGCTTTTTTATCGATGTAGTGCATATGTATCACATTTTACAGCAGCTTGGATTTGTTTATA TTCTTCATTCGTTAATGAAC
Product: 2,5-didehydrogluconate reductase
Products: 2-keto-L-gulonate; NADP [C]
Alternate protein names: NA
Number of amino acids: Translated: 275; Mature: 275
Protein sequence:
>275_residues MYIPTTTLHNGVKMPMLGLGVYKAKEGEEVKRAVKTALEIGYRSIDTAAVYENESGVGEAVRNSGIPREELFITTKVWND YQGYEETLQAFEKSLEKLQMDYVDLYLIHWPVRGKYIDTYRALEKLYKEGKVRAIGVSNFHQHHLERLLANCHVKPMVNQ VELHPMFAQFELRDFCQNKQIQMEAWSPLMRGREVFEHPIIQEIASKYNKTPAQIILRWDIQSGIVTIPKSVTPSRIKEN FTIFDFSLTEDEMKQIDTLDCNVRIGTNPDKYDTM
Sequences:
>Translated_275_residues MYIPTTTLHNGVKMPMLGLGVYKAKEGEEVKRAVKTALEIGYRSIDTAAVYENESGVGEAVRNSGIPREELFITTKVWND YQGYEETLQAFEKSLEKLQMDYVDLYLIHWPVRGKYIDTYRALEKLYKEGKVRAIGVSNFHQHHLERLLANCHVKPMVNQ VELHPMFAQFELRDFCQNKQIQMEAWSPLMRGREVFEHPIIQEIASKYNKTPAQIILRWDIQSGIVTIPKSVTPSRIKEN FTIFDFSLTEDEMKQIDTLDCNVRIGTNPDKYDTM >Mature_275_residues MYIPTTTLHNGVKMPMLGLGVYKAKEGEEVKRAVKTALEIGYRSIDTAAVYENESGVGEAVRNSGIPREELFITTKVWND YQGYEETLQAFEKSLEKLQMDYVDLYLIHWPVRGKYIDTYRALEKLYKEGKVRAIGVSNFHQHHLERLLANCHVKPMVNQ VELHPMFAQFELRDFCQNKQIQMEAWSPLMRGREVFEHPIIQEIASKYNKTPAQIILRWDIQSGIVTIPKSVTPSRIKEN FTIFDFSLTEDEMKQIDTLDCNVRIGTNPDKYDTM
Specific function: Reduction [C]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497577, Length=298, Percent_Identity=41.2751677852349, Blast_Score=239, Evalue=3e-63, Organism=Homo sapiens, GI5174391, Length=298, Percent_Identity=41.2751677852349, Blast_Score=239, Evalue=3e-63, Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=40.7534246575342, Blast_Score=213, Evalue=1e-55, Organism=Homo sapiens, GI310109922, Length=281, Percent_Identity=41.2811387900356, Blast_Score=206, Evalue=2e-53, Organism=Homo sapiens, GI45446745, Length=301, Percent_Identity=39.8671096345515, Blast_Score=201, Evalue=7e-52, Organism=Homo sapiens, GI4503285, Length=301, Percent_Identity=39.8671096345515, Blast_Score=201, Evalue=7e-52, Organism=Homo sapiens, GI5453543, Length=301, Percent_Identity=39.5348837209302, Blast_Score=201, Evalue=8e-52, Organism=Homo sapiens, GI310109920, Length=301, Percent_Identity=39.5348837209302, Blast_Score=200, Evalue=9e-52, Organism=Homo sapiens, GI93277124, Length=304, Percent_Identity=36.8421052631579, Blast_Score=200, Evalue=1e-51, Organism=Homo sapiens, GI5174695, Length=297, Percent_Identity=39.3939393939394, Blast_Score=197, Evalue=1e-50, Organism=Homo sapiens, GI4502049, Length=293, Percent_Identity=41.6382252559727, Blast_Score=195, Evalue=5e-50, Organism=Homo sapiens, GI291291012, Length=281, Percent_Identity=39.1459074733096, Blast_Score=193, Evalue=1e-49, Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=38.4353741496599, Blast_Score=192, Evalue=3e-49, Organism=Homo sapiens, GI24497585, Length=296, Percent_Identity=38.1756756756757, Blast_Score=184, Evalue=1e-46, Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=36.7272727272727, Blast_Score=167, Evalue=1e-41, Organism=Homo sapiens, GI300116271, Length=275, Percent_Identity=37.4545454545455, Blast_Score=167, Evalue=1e-41, Organism=Homo sapiens, GI310109924, Length=157, Percent_Identity=41.4012738853503, Blast_Score=118, Evalue=7e-27, Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=45.6140350877193, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=44.7368421052632, Blast_Score=96, Evalue=5e-20, Organism=Escherichia coli, GI87082198, Length=276, Percent_Identity=50.7246376811594, Blast_Score=290, Evalue=1e-79, Organism=Escherichia coli, GI1786400, Length=258, Percent_Identity=36.4341085271318, Blast_Score=177, Evalue=8e-46, Organism=Escherichia coli, GI1788081, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=6e-32, Organism=Escherichia coli, GI1788070, Length=303, Percent_Identity=24.7524752475248, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI87081735, Length=182, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17537077, Length=297, Percent_Identity=41.4141414141414, Blast_Score=214, Evalue=3e-56, Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=41.4814814814815, Blast_Score=209, Evalue=1e-54, Organism=Caenorhabditis elegans, GI17537075, Length=305, Percent_Identity=39.672131147541, Blast_Score=206, Evalue=1e-53, Organism=Caenorhabditis elegans, GI17564128, Length=303, Percent_Identity=37.2937293729373, Blast_Score=206, Evalue=1e-53, Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=38.1481481481481, Blast_Score=190, Evalue=6e-49, Organism=Caenorhabditis elegans, GI17550248, Length=283, Percent_Identity=38.86925795053, Blast_Score=187, Evalue=5e-48, Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=38.2352941176471, Blast_Score=181, Evalue=4e-46, Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=36.551724137931, Blast_Score=176, Evalue=9e-45, Organism=Caenorhabditis elegans, GI17537079, Length=304, Percent_Identity=32.5657894736842, Blast_Score=167, Evalue=4e-42, Organism=Caenorhabditis elegans, GI17562292, Length=273, Percent_Identity=34.4322344322344, Blast_Score=165, Evalue=2e-41, Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=30.7692307692308, Blast_Score=162, Evalue=2e-40, Organism=Caenorhabditis elegans, GI17561300, Length=276, Percent_Identity=34.7826086956522, Blast_Score=148, Evalue=3e-36, Organism=Saccharomyces cerevisiae, GI6324694, Length=276, Percent_Identity=42.3913043478261, Blast_Score=217, Evalue=2e-57, Organism=Saccharomyces cerevisiae, GI6320576, Length=286, Percent_Identity=40.2097902097902, Blast_Score=201, Evalue=8e-53, Organism=Saccharomyces cerevisiae, GI6319625, Length=289, Percent_Identity=40.1384083044983, Blast_Score=199, Evalue=5e-52, Organism=Saccharomyces cerevisiae, GI6321896, Length=309, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=1e-45, Organism=Saccharomyces cerevisiae, GI6322556, Length=264, Percent_Identity=34.0909090909091, Blast_Score=170, Evalue=2e-43, Organism=Saccharomyces cerevisiae, GI6320079, Length=282, Percent_Identity=34.3971631205674, Blast_Score=142, Evalue=5e-35, Organism=Drosophila melanogaster, GI24663317, Length=294, Percent_Identity=40.8163265306122, Blast_Score=225, Evalue=2e-59, Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=39.3333333333333, Blast_Score=216, Evalue=2e-56, Organism=Drosophila melanogaster, GI24662789, Length=303, Percent_Identity=40.2640264026403, Blast_Score=215, Evalue=2e-56, Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=39.6551724137931, Blast_Score=211, Evalue=4e-55, Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=36.875, Blast_Score=208, Evalue=4e-54, Organism=Drosophila melanogaster, GI20129731, Length=281, Percent_Identity=40.2135231316726, Blast_Score=204, Evalue=4e-53, Organism=Drosophila melanogaster, GI45553081, Length=293, Percent_Identity=39.5904436860068, Blast_Score=203, Evalue=1e-52, Organism=Drosophila melanogaster, GI21356425, Length=295, Percent_Identity=38.3050847457627, Blast_Score=200, Evalue=9e-52, Organism=Drosophila melanogaster, GI24662781, Length=298, Percent_Identity=39.9328859060403, Blast_Score=193, Evalue=1e-49, Organism=Drosophila melanogaster, GI24662785, Length=295, Percent_Identity=39.6610169491525, Blast_Score=187, Evalue=6e-48, Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=38.1322957198444, Blast_Score=172, Evalue=2e-43,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: NA
Molecular weight: Translated: 31913; Mature: 31913
Theoretical pI: Translated: 6.52; Mature: 6.52
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.6 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 3.6 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYIPTTTLHNGVKMPMLGLGVYKAKEGEEVKRAVKTALEIGYRSIDTAAVYENESGVGEA CCCCCCCCCCCCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCCCCHHEEECCCCCHHHH VRNSGIPREELFITTKVWNDYQGYEETLQAFEKSLEKLQMDYVDLYLIHWPVRGKYIDTY HHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHH RALEKLYKEGKVRAIGVSNFHQHHLERLLANCHVKPMVNQVELHPMFAQFELRDFCQNKQ HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCCC IQMEAWSPLMRGREVFEHPIIQEIASKYNKTPAQIILRWDIQSGIVTIPKSVTPSRIKEN EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCEEECCCCCCHHHHHCC FTIFDFSLTEDEMKQIDTLDCNVRIGTNPDKYDTM CEEEEEECCHHHHHHHCCCCCEEEECCCCCCCCCC >Mature Secondary Structure MYIPTTTLHNGVKMPMLGLGVYKAKEGEEVKRAVKTALEIGYRSIDTAAVYENESGVGEA CCCCCCCCCCCCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCCCCHHEEECCCCCHHHH VRNSGIPREELFITTKVWNDYQGYEETLQAFEKSLEKLQMDYVDLYLIHWPVRGKYIDTY HHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHH RALEKLYKEGKVRAIGVSNFHQHHLERLLANCHVKPMVNQVELHPMFAQFELRDFCQNKQ HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCCC IQMEAWSPLMRGREVFEHPIIQEIASKYNKTPAQIILRWDIQSGIVTIPKSVTPSRIKEN EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCEEECCCCCCHHHHHCC FTIFDFSLTEDEMKQIDTLDCNVRIGTNPDKYDTM CEEEEEECCHHHHHHHCCCCCEEEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: 2,5-diketo-D-gluconate; NADPH [C]
Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]