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The map label for this gene is sigF [H]

Identifier: 152976482

GI number: 152976482

Start: 2853070

End: 2853828

Strand: Reverse

Name: sigF [H]

Synonym: Bcer98_2771

Alternate gene names: 152976482

Gene position: 2853828-2853070 (Counterclockwise)

Preceding gene: 152976483

Following gene: 152976481

Centisome position: 69.83

GC content: 38.21

Gene sequence:

>759_bases
ATGGACATAGAGGTCAGAAATGAGAAGAAGAATCCTCAGTTAAAGGACCACGAGTTAAAAGCGTTAATTCAAAAAAGTCA
AGATGGAGATCAAGGGGCGAGAGATACGATTGTTCAAAGTAATATGCGCCTTGTATGGTCAGTTGTACAACGTTTTCTCA
ATCGAGGATATGAACCCGACGACTTATTTCAAATTGGCTGTATTGGCCTCTTGAAATCAGTAGACAAATTTGATTTATCT
TTTGATGTGAAGTTTTCAACTTACGCAGTTCCAATGATTATTGGTGAAATTCAACGTTTTTTACGTGATGACGGTTCAGT
AAAAGTGAGCCGATCGTTAAAAGAAACGGGTAATAAGATTCGAAAGATGAAAGATGAGCTTTCGAAAGAATACGGAAGGG
CACCAACCATTAATGAAGTTGCAGAGGCGCTTGAACTAACACCGGAAGAAGTTGTTCTGGCGCAAGAGGCAAGTCGTACG
CCATCTTCTATACATGAAACTGTATATGAAAATGATGGTGATCCAATCACGATTTTGGATCAAATTGCGGATCAAGCGGA
AACGAAATGGTTTGATAAGATTGCTTTAAAAGAAGCAATAAGAGAACTAGATGAGCGAGAACGCTTAATTGTATATTTGC
GCTATTATAAAGATCAAACTCAATCGGAAGTAGCAGAGAGAATTGGAATTTCACAAGTGCAAGTTTCGAGGTTGGAAAAG
AAAATATTGAAGCAGATGAAAGATCGGATAGATGAGTAG

Upstream 100 bases:

>100_bases
TGAAGTAGAAATTATTTCAAACGAATCTTTCGGGACAACAATCCATTTGACAAAATATTTATCAAATAGTAACGCTCTAT
GCAATTAAGGAGAATTGCCT

Downstream 100 bases:

>100_bases
GTATCTATCCGATTTCTTTCTGTTATTTCAAGGTAGTGAGACTCCCGCACTAAGCTCATGTAGAGGGAAAGGAACGAGTT
ATATAAATCGGATTTATAAA

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDIEVRNEKKNPQLKDHELKALIQKSQDGDQGARDTIVQSNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
FDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKIRKMKDELSKEYGRAPTINEVAEALELTPEEVVLAQEASRT
PSSIHETVYENDGDPITILDQIADQAETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
KILKQMKDRIDE

Sequences:

>Translated_252_residues
MDIEVRNEKKNPQLKDHELKALIQKSQDGDQGARDTIVQSNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
FDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKIRKMKDELSKEYGRAPTINEVAEALELTPEEVVLAQEASRT
PSSIHETVYENDGDPITILDQIADQAETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
KILKQMKDRIDE
>Mature_252_residues
MDIEVRNEKKNPQLKDHELKALIQKSQDGDQGARDTIVQSNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
FDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKIRKMKDELSKEYGRAPTINEVAEALELTPEEVVLAQEASRT
PSSIHETVYENDGDPITILDQIADQAETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
KILKQMKDRIDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=29.2035398230088, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789098, Length=250, Percent_Identity=25.2, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=28.8557213930348, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29122; Mature: 29122

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEVRNEKKNPQLKDHELKALIQKSQDGDQGARDTIVQSNMRLVWSVVQRFLNRGYEPD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DLFQIGCIGLLKSVDKFDLSFDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKI
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
RKMKDELSKEYGRAPTINEVAEALELTPEEVVLAQEASRTPSSIHETVYENDGDPITILD
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHH
QIADQAETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILKQMKDRIDE
HHHHHHHHHHCC
>Mature Secondary Structure
MDIEVRNEKKNPQLKDHELKALIQKSQDGDQGARDTIVQSNMRLVWSVVQRFLNRGYEPD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DLFQIGCIGLLKSVDKFDLSFDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKI
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
RKMKDELSKEYGRAPTINEVAEALELTPEEVVLAQEASRTPSSIHETVYENDGDPITILD
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHH
QIADQAETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILKQMKDRIDE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]