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The map label for this gene is ykwC [H]

Identifier: 152976452

GI number: 152976452

Start: 2824672

End: 2825550

Strand: Reverse

Name: ykwC [H]

Synonym: Bcer98_2741

Alternate gene names: 152976452

Gene position: 2825550-2824672 (Counterclockwise)

Preceding gene: 152976453

Following gene: 152976451

Centisome position: 69.13

GC content: 36.18

Gene sequence:

>879_bases
ATGGAGCATAACATTTCATCAATTGGTTTTATTGGAACAGGTGTAATGGGAAAAAGCATGGTACATCATTTATTAAAAGG
TGGTTATACAGTATATGTGTATAATCGTACGAAAGAAAAAGCAACTTCTTTAGTAAGAGAGGGGGCGAAATGGTGTGGTA
CGCCGAAAGATTTGGTGAAAAATGTTGATGTTGTTATGACAATGGTAGGATATCCACATGATGTAGAAGAAATATATTTT
GGAGTAGATGGAATTTTAGCAAATGCAAAGGAAGGGATGATTGCGATTGATTTCACTACTTCTACACCAACATTAGCCAA
AAGGATATATGAAGTTGGGAAAGAAAAAAATATATATGCTTTAGACGCTCCAGTCTCAGGAGGAGATATTGGTGCAAAGG
AAGGGAAACTTGCTATTATGATTGGTGGAGACCAAGAAGTATATGAAGTTTGTTTCCCGCTTTTTAACATGTTAGGTGAA
AATATTCAATTGCAAGGGCCAGCTGGGAGTGGTCAACATACAAAGATGTGTAATCAAATTGCAATTGCATCGAATATGAT
AGGTGTTTGTGAAGCGGTAGCATATGCACAAAAAGCCGGTTTAGACCCAGAGAAAGTATTGCAAAGCATTGCAACTGGAG
CAGCTGGAAGTTGGTCTTTAAGCAATTTGGCTCCGCGTATGTTAAAAGGAGATTTTGAACCAGGTTTTTATGTAAAACAT
TTTATGAAAGATATGAAAATTGCATTAGATGAAGCAGAAAAGTTACAATTATCTGTACCTGGTTTATCGTTAGCAAAAGA
TTTATATGAGCAGTTAATTGAGGCTGGGGAAAGCAATAGTGGAACACAGGTGTTATATAAAAAATATATAAGGGGGTAA

Upstream 100 bases:

>100_bases
GTTATTTTATTCTCGAATGTAAAAAATAGTATGGATGATGATTTTAATAAAGAGTTCCGAAAAATGGTTAGCGATTTATT
AGAACAAAGGGGATGAGAAT

Downstream 100 bases:

>100_bases
CATCAATGGATTTAGTGAAATTTGATGGAATGATTGATGCAGTGCAGAGAGCAACTTGCATACCGATTCATGATAAGCAA
AAAGAAGCTTTTAAACAAAA

Product: 6-phosphogluconate dehydrogenase NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEHNISSIGFIGTGVMGKSMVHHLLKGGYTVYVYNRTKEKATSLVREGAKWCGTPKDLVKNVDVVMTMVGYPHDVEEIYF
GVDGILANAKEGMIAIDFTTSTPTLAKRIYEVGKEKNIYALDAPVSGGDIGAKEGKLAIMIGGDQEVYEVCFPLFNMLGE
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAQKAGLDPEKVLQSIATGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALDEAEKLQLSVPGLSLAKDLYEQLIEAGESNSGTQVLYKKYIRG

Sequences:

>Translated_292_residues
MEHNISSIGFIGTGVMGKSMVHHLLKGGYTVYVYNRTKEKATSLVREGAKWCGTPKDLVKNVDVVMTMVGYPHDVEEIYF
GVDGILANAKEGMIAIDFTTSTPTLAKRIYEVGKEKNIYALDAPVSGGDIGAKEGKLAIMIGGDQEVYEVCFPLFNMLGE
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAQKAGLDPEKVLQSIATGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALDEAEKLQLSVPGLSLAKDLYEQLIEAGESNSGTQVLYKKYIRG
>Mature_292_residues
MEHNISSIGFIGTGVMGKSMVHHLLKGGYTVYVYNRTKEKATSLVREGAKWCGTPKDLVKNVDVVMTMVGYPHDVEEIYF
GVDGILANAKEGMIAIDFTTSTPTLAKRIYEVGKEKNIYALDAPVSGGDIGAKEGKLAIMIGGDQEVYEVCFPLFNMLGE
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAQKAGLDPEKVLQSIATGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALDEAEKLQLSVPGLSLAKDLYEQLIEAGESNSGTQVLYKKYIRG

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=28.6219081272085, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=28.6689419795222, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=36.6197183098592, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI145693186, Length=265, Percent_Identity=35.4716981132075, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1790315, Length=275, Percent_Identity=27.2727272727273, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789092, Length=274, Percent_Identity=25.9124087591241, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=31.1418685121107, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=31.1418685121107, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=28.1138790035587, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31601; Mature: 31601

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHNISSIGFIGTGVMGKSMVHHLLKGGYTVYVYNRTKEKATSLVREGAKWCGTPKDLVK
CCCCCCCCCHHHCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHH
NVDVVMTMVGYPHDVEEIYFGVDGILANAKEGMIAIDFTTSTPTLAKRIYEVGKEKNIYA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEE
LDAPVSGGDIGAKEGKLAIMIGGDQEVYEVCFPLFNMLGENIQLQGPAGSGQHTKMCNQI
EECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
AIASNMIGVCEAVAYAQKAGLDPEKVLQSIATGAAGSWSLSNLAPRMLKGDFEPGFYVKH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCHHHHCCCCCCCHHHHH
FMKDMKIALDEAEKLQLSVPGLSLAKDLYEQLIEAGESNSGTQVLYKKYIRG
HHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEHNISSIGFIGTGVMGKSMVHHLLKGGYTVYVYNRTKEKATSLVREGAKWCGTPKDLVK
CCCCCCCCCHHHCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHH
NVDVVMTMVGYPHDVEEIYFGVDGILANAKEGMIAIDFTTSTPTLAKRIYEVGKEKNIYA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEE
LDAPVSGGDIGAKEGKLAIMIGGDQEVYEVCFPLFNMLGENIQLQGPAGSGQHTKMCNQI
EECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
AIASNMIGVCEAVAYAQKAGLDPEKVLQSIATGAAGSWSLSNLAPRMLKGDFEPGFYVKH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCHHHHCCCCCCCHHHHH
FMKDMKIALDEAEKLQLSVPGLSLAKDLYEQLIEAGESNSGTQVLYKKYIRG
HHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]