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The map label for this gene is ppx [H]

Identifier: 152976408

GI number: 152976408

Start: 2779850

End: 2781388

Strand: Reverse

Name: ppx [H]

Synonym: Bcer98_2696

Alternate gene names: 152976408

Gene position: 2781388-2779850 (Counterclockwise)

Preceding gene: 152976410

Following gene: 152976407

Centisome position: 68.05

GC content: 32.88

Gene sequence:

>1539_bases
ATGAAAGATATATTGAAACAGCAATATGCCATTATCGATATCGGTTCGAATACGATGAGGTTGGTAATTTATGAAAGGCA
AAATGGTGGCTTTTATAAGGAGATTGAAAATATAAAAGTTGTTGCTCGTCTAAGAAATTATTTGCGAGGCGGTCTATTGA
TAGAAGAAGGCACAAAAATATTACTTCAAACATTGCGCCAATTTCAAGAAAGTACAAGGTTCTATCATATACAAAATGTA
CTTTGTGTTGCTACAGCGACTATTCGTCAAGCTAAAAATCAAGAGGAAATAAAAAGGGTTGTTGCGAGAGAAACAGACTT
TACACTTCGGATTTTGTCTGAATATGAAGAAGCTCGTTATGGGTATTTAGCAGTCATGAACTCCACTTCATTTTGTGAAG
GGATTACTGTTGATATTGGCGGAGGAAGTACAGAAGTTACATATTTTAAAGATAGAGAAATTGTAGAATATTATAGTTTT
TCATTTGGAGCTCTTTCTTTGAAACAACAATTTATTCAGCATGAGATACCAACTACAGAAGAATTGAAGAAATTGAGAAA
CTATCTATATGATCAATTTCAAACATTGCCGTGGTTAAGAGGAAAAAAACTACCCCTTATTGCGATTGGTGGTAGTGCTA
GAAATTTGGTCAAAATTCATCAAAACTTAATTTCCTATCCTTTAGCAGGCCTACATCTATACAAGATGAAAGAAAAGGAT
ATAAAGGATGTACAAGAAGAGTTGATGAAGCTTTCTTTTGCTGATTTACAAAAGTTAGATGGATTAGCAAAAGACAGAGC
AGATACAATCATTCCAGCGGTTGAAGTCTTTCATATTCTCACAAATATAATTGAAGCACCAGCATTTGTATTAAGTAGGA
AAGGTTTGCGAGAAGGTGTATTTTATGAAGAATTAACAAGGGGATTTGGAATTTTTTATTATCCAAATGTGATAGAAGAA
AGCTTATATTTATTGTCACATGAATATGAAATAAATATGGGTTTTGTTATTCAGCTTATGAAACAAGGAGCGGTTATTTG
TAAGCAGCTTGAGCAAGCTGGACTTATTTCTTTATCAGAACAAGATTGGAAAGCGTTTTATCAAGCAGCTAAAGTATTTA
ATATTGGGAAATATATTGATGAAGAAGCAAGCTGTTTGCATACATTTTATTTGTTAGCGAATAAAACGATTGACGGTATG
ATGCATAAAGAACGAATTAGATTAGCGCTTATTGCTTCATATAAGTCGAAGGTGTTATTTAAGCAACATTTAGCACCATT
TGAAGATTGGTTTGACAAAAATGAACAGAAAAAAATGCGCCTTTTAGGAGCGGTATTGCAATTTTCAGCTGCTTTAAATG
TAAGGCAGAGCCAGCTTATCGAAAACATACACATAGAACGAAATAAAGATGCACTTGTATTTCAAGTCTTTTGTAAGCAA
GCTGTTTTAGCGGAAATAGTGGAAGCAGAGAAACAAAAAAAGCAGTTAGAAAAAATATTAAAAATGAACATTCAGTTGAT
ATTTACAAAGGAATGTTAA

Upstream 100 bases:

>100_bases
TATGCTTAATATTAAAGCAATCTTGTTCCAAAAAACAACTATTTTGTATAATAGTGATAGGAAGGGGTTTTAGAAATTTG
ATTATGAAAGAGTGAAAAAA

Downstream 100 bases:

>100_bases
GAAGTACGGATGTCTTTACAAAAATTTTACACTTTAACAAATTGAAATTTGGTACAATAAATGTACATAAATTATATTGA
AAAAGTTTCAAGGGGAAGTG

Product: Ppx/GppA phosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKDILKQQYAIIDIGSNTMRLVIYERQNGGFYKEIENIKVVARLRNYLRGGLLIEEGTKILLQTLRQFQESTRFYHIQNV
LCVATATIRQAKNQEEIKRVVARETDFTLRILSEYEEARYGYLAVMNSTSFCEGITVDIGGGSTEVTYFKDREIVEYYSF
SFGALSLKQQFIQHEIPTTEELKKLRNYLYDQFQTLPWLRGKKLPLIAIGGSARNLVKIHQNLISYPLAGLHLYKMKEKD
IKDVQEELMKLSFADLQKLDGLAKDRADTIIPAVEVFHILTNIIEAPAFVLSRKGLREGVFYEELTRGFGIFYYPNVIEE
SLYLLSHEYEINMGFVIQLMKQGAVICKQLEQAGLISLSEQDWKAFYQAAKVFNIGKYIDEEASCLHTFYLLANKTIDGM
MHKERIRLALIASYKSKVLFKQHLAPFEDWFDKNEQKKMRLLGAVLQFSAALNVRQSQLIENIHIERNKDALVFQVFCKQ
AVLAEIVEAEKQKKQLEKILKMNIQLIFTKEC

Sequences:

>Translated_512_residues
MKDILKQQYAIIDIGSNTMRLVIYERQNGGFYKEIENIKVVARLRNYLRGGLLIEEGTKILLQTLRQFQESTRFYHIQNV
LCVATATIRQAKNQEEIKRVVARETDFTLRILSEYEEARYGYLAVMNSTSFCEGITVDIGGGSTEVTYFKDREIVEYYSF
SFGALSLKQQFIQHEIPTTEELKKLRNYLYDQFQTLPWLRGKKLPLIAIGGSARNLVKIHQNLISYPLAGLHLYKMKEKD
IKDVQEELMKLSFADLQKLDGLAKDRADTIIPAVEVFHILTNIIEAPAFVLSRKGLREGVFYEELTRGFGIFYYPNVIEE
SLYLLSHEYEINMGFVIQLMKQGAVICKQLEQAGLISLSEQDWKAFYQAAKVFNIGKYIDEEASCLHTFYLLANKTIDGM
MHKERIRLALIASYKSKVLFKQHLAPFEDWFDKNEQKKMRLLGAVLQFSAALNVRQSQLIENIHIERNKDALVFQVFCKQ
AVLAEIVEAEKQKKQLEKILKMNIQLIFTKEC
>Mature_512_residues
MKDILKQQYAIIDIGSNTMRLVIYERQNGGFYKEIENIKVVARLRNYLRGGLLIEEGTKILLQTLRQFQESTRFYHIQNV
LCVATATIRQAKNQEEIKRVVARETDFTLRILSEYEEARYGYLAVMNSTSFCEGITVDIGGGSTEVTYFKDREIVEYYSF
SFGALSLKQQFIQHEIPTTEELKKLRNYLYDQFQTLPWLRGKKLPLIAIGGSARNLVKIHQNLISYPLAGLHLYKMKEKD
IKDVQEELMKLSFADLQKLDGLAKDRADTIIPAVEVFHILTNIIEAPAFVLSRKGLREGVFYEELTRGFGIFYYPNVIEE
SLYLLSHEYEINMGFVIQLMKQGAVICKQLEQAGLISLSEQDWKAFYQAAKVFNIGKYIDEEASCLHTFYLLANKTIDGM
MHKERIRLALIASYKSKVLFKQHLAPFEDWFDKNEQKKMRLLGAVLQFSAALNVRQSQLIENIHIERNKDALVFQVFCKQ
AVLAEIVEAEKQKKQLEKILKMNIQLIFTKEC

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=444, Percent_Identity=24.7747747747748, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 59324; Mature: 59324

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDILKQQYAIIDIGSNTMRLVIYERQNGGFYKEIENIKVVARLRNYLRGGLLIEEGTKI
CCHHHHHCEEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHH
LLQTLRQFQESTRFYHIQNVLCVATATIRQAKNQEEIKRVVARETDFTLRILSEYEEARY
HHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
GYLAVMNSTSFCEGITVDIGGGSTEVTYFKDREIVEYYSFSFGALSLKQQFIQHEIPTTE
CEEEEECCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCHH
ELKKLRNYLYDQFQTLPWLRGKKLPLIAIGGSARNLVKIHQNLISYPLAGLHLYKMKEKD
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEHHHHHH
IKDVQEELMKLSFADLQKLDGLAKDRADTIIPAVEVFHILTNIIEAPAFVLSRKGLREGV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
FYEELTRGFGIFYYPNVIEESLYLLSHEYEINMGFVIQLMKQGAVICKQLEQAGLISLSE
HHHHHHCCCCEEECHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHHHCCCEECCH
QDWKAFYQAAKVFNIGKYIDEEASCLHTFYLLANKTIDGMMHKERIRLALIASYKSKVLF
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
KQHLAPFEDWFDKNEQKKMRLLGAVLQFSAALNVRQSQLIENIHIERNKDALVFQVFCKQ
HHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AVLAEIVEAEKQKKQLEKILKMNIQLIFTKEC
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure
MKDILKQQYAIIDIGSNTMRLVIYERQNGGFYKEIENIKVVARLRNYLRGGLLIEEGTKI
CCHHHHHCEEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHH
LLQTLRQFQESTRFYHIQNVLCVATATIRQAKNQEEIKRVVARETDFTLRILSEYEEARY
HHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
GYLAVMNSTSFCEGITVDIGGGSTEVTYFKDREIVEYYSFSFGALSLKQQFIQHEIPTTE
CEEEEECCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCHH
ELKKLRNYLYDQFQTLPWLRGKKLPLIAIGGSARNLVKIHQNLISYPLAGLHLYKMKEKD
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEHHHHHH
IKDVQEELMKLSFADLQKLDGLAKDRADTIIPAVEVFHILTNIIEAPAFVLSRKGLREGV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
FYEELTRGFGIFYYPNVIEESLYLLSHEYEINMGFVIQLMKQGAVICKQLEQAGLISLSE
HHHHHHCCCCEEECHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHHHCCCEECCH
QDWKAFYQAAKVFNIGKYIDEEASCLHTFYLLANKTIDGMMHKERIRLALIASYKSKVLF
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
KQHLAPFEDWFDKNEQKKMRLLGAVLQFSAALNVRQSQLIENIHIERNKDALVFQVFCKQ
HHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AVLAEIVEAEKQKKQLEKILKMNIQLIFTKEC
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]