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The map label for this gene is dapL

Identifier: 152976394

GI number: 152976394

Start: 2766579

End: 2767709

Strand: Reverse

Name: dapL

Synonym: Bcer98_2682

Alternate gene names: 152976394

Gene position: 2767709-2766579 (Counterclockwise)

Preceding gene: 152976395

Following gene: 152976393

Centisome position: 67.72

GC content: 38.55

Gene sequence:

>1131_bases
ATGACAATCAGTAAATTTGTACAAATCCGTAGGGATTTACATCAAATTCCTGAATTAGGATTTCAAGAGTGGAAAACACA
GCAATATATTTTAAATTATATTGAGACGCTTCCTAACGAACATATTGAAGTAAAGACATGGAAAACAGGGGTTATCGTTA
AAGTCAAGGGAAAGAATCCGGTAAAAACAATTGGTTACCGCGCGGATATGGATGGTTTGCCAATTGTAGAGGAAACAGGA
TATGAATTCGCTTCGACTCATGAAGGAATGATGCATGCGTGCGGTCATGATTTTCATACTACAATTGGTTTAGGATTGCT
TACCGCCACTGTAAATGACAGAATAGATGATGACCTTGTTTTTCTATTCCAACCTGCTGAAGAAGGGCCTGGCGGAGCAC
TTCCTATGTTAGAAAGCGAAGAATTAAAAGAATGGAAACCAAATATGATTCTTGGGCTTCATATTGCACCAGAATATCCA
GTCGGAACGATTGCTACAAAAGAAGGGTTACTCTTTGCAAATACATCCGAATTGTATATTGATTTAAAGGGAAAAGGTGG
ACATGCGGCATACCCACATATGGCGAATGATATGATTGTTGCAGCTAGTCATCTTGTGACCCAGCTTCAATCTGTAATTA
GTCGTAATGTGAATCCATTGGATAGTGCAGTGATTACAATTGGTAAAATTACAGGTGGAACCGTTCAAAATATAATTGCA
GAAAAGTCGCGTTTAGAGGGGACAATTCGAACATTATCTGTTGAATCAATGAAGCGAGTGAAAGATAGAATCGAAGCGAT
TGTTGCTGGGATTGAGGCTGCATTCCAATGTGAGGCGGTTATTGATTATGGTGCAATGTATCATCAAGTATATAATCATG
AAGCATTAACCAAAGAATTTATGGAGTTCGCTTCAAAAGATACGAATATGAATGTGGTTACTTGCAAGGAGGCTATGACG
GGAGAAGATTTCGGATACATGCTTCGCGATATTCCAGGATTTATGTTTTGGCTTGGTGTCGATTCTGAATATGGTTTGCA
TCATGCGAAACTAAAGCCAAATGAAGCGGCAATTGATCGAGCAATTGAATTTTTAAATCAATATGTCAAATGGAAAGGGA
ACAGGAGGTAG

Upstream 100 bases:

>100_bases
TTAAGCAAGAGCTTCGTCAATTAAACCCAGAAAAATAAAAAAGCAAAAAGCGTAAGGGAATGAAGAGGCCCTTACGCTTT
TTATAAATGGCGGTGTAGAC

Downstream 100 bases:

>100_bases
ACCTGTTCTTTTTTTACGGCACGATTTTAAAGAGCGATCCTCGGTGAAGTAGATAAAGGATTTTAAAAATATGTTCTATG
AAAAATTAGAATGGGAGTAA

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTISKFVQIRRDLHQIPELGFQEWKTQQYILNYIETLPNEHIEVKTWKTGVIVKVKGKNPVKTIGYRADMDGLPIVEETG
YEFASTHEGMMHACGHDFHTTIGLGLLTATVNDRIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNMILGLHIAPEYP
VGTIATKEGLLFANTSELYIDLKGKGGHAAYPHMANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EKSRLEGTIRTLSVESMKRVKDRIEAIVAGIEAAFQCEAVIDYGAMYHQVYNHEALTKEFMEFASKDTNMNVVTCKEAMT
GEDFGYMLRDIPGFMFWLGVDSEYGLHHAKLKPNEAAIDRAIEFLNQYVKWKGNRR

Sequences:

>Translated_376_residues
MTISKFVQIRRDLHQIPELGFQEWKTQQYILNYIETLPNEHIEVKTWKTGVIVKVKGKNPVKTIGYRADMDGLPIVEETG
YEFASTHEGMMHACGHDFHTTIGLGLLTATVNDRIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNMILGLHIAPEYP
VGTIATKEGLLFANTSELYIDLKGKGGHAAYPHMANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EKSRLEGTIRTLSVESMKRVKDRIEAIVAGIEAAFQCEAVIDYGAMYHQVYNHEALTKEFMEFASKDTNMNVVTCKEAMT
GEDFGYMLRDIPGFMFWLGVDSEYGLHHAKLKPNEAAIDRAIEFLNQYVKWKGNRR
>Mature_375_residues
TISKFVQIRRDLHQIPELGFQEWKTQQYILNYIETLPNEHIEVKTWKTGVIVKVKGKNPVKTIGYRADMDGLPIVEETGY
EFASTHEGMMHACGHDFHTTIGLGLLTATVNDRIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNMILGLHIAPEYPV
GTIATKEGLLFANTSELYIDLKGKGGHAAYPHMANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIAE
KSRLEGTIRTLSVESMKRVKDRIEAIVAGIEAAFQCEAVIDYGAMYHQVYNHEALTKEFMEFASKDTNMNVVTCKEAMTG
EDFGYMLRDIPGFMFWLGVDSEYGLHHAKLKPNEAAIDRAIEFLNQYVKWKGNRR

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily

Homologues:

Organism=Escherichia coli, GI87081880, Length=232, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPL_BACCN (A7GS08)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001375911.1
- ProteinModelPortal:   A7GS08
- SMR:   A7GS08
- STRING:   A7GS08
- EnsemblBacteria:   EBBACT00000038001
- GeneID:   5344016
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2682
- eggNOG:   COG1473
- GeneTree:   EBGT00070000031832
- HOGENOM:   HBG708500
- OMA:   IDYGSNY
- ProtClustDB:   CLSK873387
- BioCyc:   BCER315749:BCER98_2682-MONOMER
- HAMAP:   MF_01692
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.47

Molecular weight: Translated: 42119; Mature: 41988

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISKFVQIRRDLHQIPELGFQEWKTQQYILNYIETLPNEHIEVKTWKTGVIVKVKGKNP
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCC
VKTIGYRADMDGLPIVEETGYEFASTHEGMMHACGHDFHTTIGLGLLTATVNDRIDDDLV
CHHCCEECCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEHHCCCCCCCEE
FLFQPAEEGPGGALPMLESEELKEWKPNMILGLHIAPEYPVGTIATKEGLLFANTSELYI
EEEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCEEEEECCEEEE
DLKGKGGHAAYPHMANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHH
EKSRLEGTIRTLSVESMKRVKDRIEAIVAGIEAAFQCEAVIDYGAMYHQVYNHEALTKEF
HHHHHCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEFASKDTNMNVVTCKEAMTGEDFGYMLRDIPGFMFWLGVDSEYGLHHAKLKPNEAAIDR
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AIEFLNQYVKWKGNRR
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TISKFVQIRRDLHQIPELGFQEWKTQQYILNYIETLPNEHIEVKTWKTGVIVKVKGKNP
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCC
VKTIGYRADMDGLPIVEETGYEFASTHEGMMHACGHDFHTTIGLGLLTATVNDRIDDDLV
CHHCCEECCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEHHCCCCCCCEE
FLFQPAEEGPGGALPMLESEELKEWKPNMILGLHIAPEYPVGTIATKEGLLFANTSELYI
EEEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCEEEEECCEEEE
DLKGKGGHAAYPHMANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHH
EKSRLEGTIRTLSVESMKRVKDRIEAIVAGIEAAFQCEAVIDYGAMYHQVYNHEALTKEF
HHHHHCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEFASKDTNMNVVTCKEAMTGEDFGYMLRDIPGFMFWLGVDSEYGLHHAKLKPNEAAIDR
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AIEFLNQYVKWKGNRR
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA