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The map label for this gene is carA [H]

Identifier: 152976248

GI number: 152976248

Start: 2636793

End: 2637914

Strand: Reverse

Name: carA [H]

Synonym: Bcer98_2536

Alternate gene names: 152976248

Gene position: 2637914-2636793 (Counterclockwise)

Preceding gene: 152976249

Following gene: 152976247

Centisome position: 64.54

GC content: 37.43

Gene sequence:

>1122_bases
ATGGCAAAAGGAGAGTGCATTAGTATGAAAAGACAGCTTATCTTAGAAGATGGAACAGTATTAATTGGAAAAGGATTCGG
CGGAGAGATTGAAAAATCAGGTGAAGTTGTATTTACAACTGGTATGACCGGATACCAAGAAACATTATCTGATCCATCAT
ACTGCGGTCAAATCGTAACATTTACGTATCCATTGATCGGAAACTATGGTATTAACCGTGATGATTTTGAATCGATTCAA
CCATCTGTACACGGTTTAATTGTAAATGAAATTTGCGATCATCCATCGAACTTCCGTAATGAAAATTCGTTAGATGAGTA
TTTAAAAGAACGAAATATTCCAGGATTATCAGGAATTGATACGAGAAAATTAACGAGAAAAATTCGTCAATATGGAACAA
TGCGCGGACGTCTTTGTAATATGGATGTCGATGTAGATTATATTGTCAGTCAATTAAGAGCTACAGTATTTACAGATCAT
GTAAAGCGTGTATCAACAAAAGATCCATATCCAAGCCCAGGTCGTGGTTACCGCGTTGTCCTTGTTGATTTCGGAATGAA
GCACGGTATTTTACGCGAATTAAATAAGCGTGATTGCGATGTCATTGTTGTTCCTTACAATACATCGGCAGAAGAAATTT
TACGCTTAAGCCCAGATGGAATTATGTTAAGTAACGGACCTGGGGATCCAAAAGATGTACCAGAAGCGATTGAAATGTTA
AAAGGAATCATTGGAAAAGTACCTTTATTCGGTATTTGCCTTGGGCATCAACTATTTGCCTTGGCATCAGGTGCAAATAC
AGTGAAATTAAAATTTGGTCACCGTGGATTAAACCATCCAGTAAAACATCTTGAAACTGGAAAAGTAGCTATCACTTCTC
AAAACCATGGGTATGCAGTAGAAGAGGAATCTGTAAAAGGAACAGACCTTGAGATTACACACGTTGCTTTAAATGATGGA
ACAGTAGAAGGACTTCGTCATAAGAAGTTCCCAGCATTTACAGTACAATATCATCCAGAAGCATCAGCAGGACCAGAAGA
TGCAAATGACTTATTTGAAGATTTCTTAACAATGATTGCAAACTTTAAGAAAGAAGGGGAAGAGTTATGCCAAAACGCCT
AG

Upstream 100 bases:

>100_bases
TTGACCCAACAACATTCTTATCAAAAGGAAAAAATACGCCATTTGCAGGCTGGAAGTGTCAAGGATGGCCAGTTATGACA
TTAGTGGGCGGGAAGATCGT

Downstream 100 bases:

>100_bases
ACATTAACACAATTTTAGTAATCGGATCAGGACCAATTGTAATTGGGCAAGCAGCGGAGTTTGATTACTCTGGAACGCAA
GCTTGTCAATCTTTAAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MAKGECISMKRQLILEDGTVLIGKGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIQ
PSVHGLIVNEICDHPSNFRNENSLDEYLKERNIPGLSGIDTRKLTRKIRQYGTMRGRLCNMDVDVDYIVSQLRATVFTDH
VKRVSTKDPYPSPGRGYRVVLVDFGMKHGILRELNKRDCDVIVVPYNTSAEEILRLSPDGIMLSNGPGDPKDVPEAIEML
KGIIGKVPLFGICLGHQLFALASGANTVKLKFGHRGLNHPVKHLETGKVAITSQNHGYAVEEESVKGTDLEITHVALNDG
TVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIANFKKEGEELCQNA

Sequences:

>Translated_373_residues
MAKGECISMKRQLILEDGTVLIGKGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIQ
PSVHGLIVNEICDHPSNFRNENSLDEYLKERNIPGLSGIDTRKLTRKIRQYGTMRGRLCNMDVDVDYIVSQLRATVFTDH
VKRVSTKDPYPSPGRGYRVVLVDFGMKHGILRELNKRDCDVIVVPYNTSAEEILRLSPDGIMLSNGPGDPKDVPEAIEML
KGIIGKVPLFGICLGHQLFALASGANTVKLKFGHRGLNHPVKHLETGKVAITSQNHGYAVEEESVKGTDLEITHVALNDG
TVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIANFKKEGEELCQNA
>Mature_372_residues
AKGECISMKRQLILEDGTVLIGKGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIQP
SVHGLIVNEICDHPSNFRNENSLDEYLKERNIPGLSGIDTRKLTRKIRQYGTMRGRLCNMDVDVDYIVSQLRATVFTDHV
KRVSTKDPYPSPGRGYRVVLVDFGMKHGILRELNKRDCDVIVVPYNTSAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLK
GIIGKVPLFGICLGHQLFALASGANTVKLKFGHRGLNHPVKHLETGKVAITSQNHGYAVEEESVKGTDLEITHVALNDGT
VEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIANFKKEGEELCQNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=363, Percent_Identity=41.5977961432507, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI169790915, Length=370, Percent_Identity=36.4864864864865, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI21361331, Length=370, Percent_Identity=36.4864864864865, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=46.1741424802111, Blast_Score=314, Evalue=5e-87,
Organism=Escherichia coli, GI1789760, Length=151, Percent_Identity=27.8145695364238, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=365, Percent_Identity=41.9178082191781, Blast_Score=260, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=38.265306122449, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=38.5224274406332, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322638, Length=151, Percent_Identity=33.7748344370861, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=39.7932816537468, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=39.7932816537468, Blast_Score=255, Evalue=4e-68,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41248; Mature: 41116

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGECISMKRQLILEDGTVLIGKGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVT
CCCCHHHHHHHHHEEECCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCHHHCCEEEE
FTYPLIGNYGINRDDFESIQPSVHGLIVNEICDHPSNFRNENSLDEYLKERNIPGLSGID
EEEHHHCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCC
TRKLTRKIRQYGTMRGRLCNMDVDVDYIVSQLRATVFTDHVKRVSTKDPYPSPGRGYRVV
HHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LVDFGMKHGILRELNKRDCDVIVVPYNTSAEEILRLSPDGIMLSNGPGDPKDVPEAIEML
EEECCCHHHHHHHHCCCCCCEEEECCCCCHHHHHEECCCCEEEECCCCCHHHHHHHHHHH
KGIIGKVPLFGICLGHQLFALASGANTVKLKFGHRGLNHPVKHLETGKVAITSQNHGYAV
HHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCEEEEECCCCEEE
EEESVKGTDLEITHVALNDGTVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIA
CCCCCCCCCEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
NFKKEGEELCQNA
HHHHHHHHHHCCC
>Mature Secondary Structure 
AKGECISMKRQLILEDGTVLIGKGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVT
CCCHHHHHHHHHEEECCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCHHHCCEEEE
FTYPLIGNYGINRDDFESIQPSVHGLIVNEICDHPSNFRNENSLDEYLKERNIPGLSGID
EEEHHHCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCC
TRKLTRKIRQYGTMRGRLCNMDVDVDYIVSQLRATVFTDHVKRVSTKDPYPSPGRGYRVV
HHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LVDFGMKHGILRELNKRDCDVIVVPYNTSAEEILRLSPDGIMLSNGPGDPKDVPEAIEML
EEECCCHHHHHHHHCCCCCCEEEECCCCCHHHHHEECCCCEEEECCCCCHHHHHHHHHHH
KGIIGKVPLFGICLGHQLFALASGANTVKLKFGHRGLNHPVKHLETGKVAITSQNHGYAV
HHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCEEEEECCCCEEE
EEESVKGTDLEITHVALNDGTVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIA
CCCCCCCCCEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
NFKKEGEELCQNA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]