BacMap

Definition	Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome.
Accession	NC_009674
Length	4,087,024

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The map label for this gene is gmk [H]

Identifier: 152976237

GI number: 152976237

Start: 2622880

End: 2623497

Strand: Reverse

Name: gmk [H]

Synonym: Bcer98_2523

Alternate gene names: 152976237

Gene position: 2623497-2622880 (Counterclockwise)

Preceding gene: 152976238

Following gene: 152976236

Centisome position: 64.19

GC content: 37.38

Gene sequence:

>618_bases
ATGAGAAGTAGAAGAGGGTTGCTCATTGTTCTTTCAGGACCTTCGGGCGTTGGAAAAGGAACAGTTCGTAAAGAATTGTT
CAGTCATAAAGATACACAATTTCATTACTCTATTTCAGTAACGACTCGTAAACCACGTGAGGGTGAAATAGATGGTATAG
ATTATTTCTTTAAAGAAAGAGAAGAATTTGAAGAAATGATTCGTAACGACCAGTTACTTGAATGGGCAGAGTTCGTTGGA
AATTACTATGGAACGCCGATTGACTATGTTGAAAAGACTTTATCAGAAGGAAAAGATGTATTTCTAGAAATTGAAGTACA
AGGAGCAATGCAAGTAAAGAAAGCATTCCCAGAAGGAGTGTTTATCTTCTTAGCACCTCCGAGCCTATCGGAATTGAAAA
ATAGAATCGTAGGGCGTGGTACGGAGACGGAAGAAGTAATTGCAAATCGTTTAACTGTAGCAAAAGAAGAAATTGAAATG
ATGGACGCTTATGATTATGTTGTAGAAAACGATAAAGTTGAATTAGCTTGTGAACGAATTAAAGCAATTGTGGTTGGCGA
ACATTGTCGCCGCGAAAGAGTAGCACAATATTATAAGGAAATGACGGAGGGTATATAA

Upstream 100 bases:

>100_bases
GATGGACATGTTGTACTAAGTCCAATTCAACCAGAAACGATTGCACATCGTTTAAATAATAAAGAAGATTTAAGTGAGGA
AGGGTAGGTTTTACATATTT

Downstream 100 bases:

>100_bases
TATGTTAAATCCATCAATTGACTCATTACTAAAAAAAATCGACTCTAAGTATACATTAGTAACAGTAGCAGCGAAGCGCG
CTCGTGAAATGCAACTAGCG

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHKDTQFHYSISVTTRKPREGEIDGIDYFFKEREEFEEMIRNDQLLEWAEFVG
NYYGTPIDYVEKTLSEGKDVFLEIEVQGAMQVKKAFPEGVFIFLAPPSLSELKNRIVGRGTETEEVIANRLTVAKEEIEM
MDAYDYVVENDKVELACERIKAIVVGEHCRRERVAQYYKEMTEGI

Sequences:

>Translated_205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHKDTQFHYSISVTTRKPREGEIDGIDYFFKEREEFEEMIRNDQLLEWAEFVG
NYYGTPIDYVEKTLSEGKDVFLEIEVQGAMQVKKAFPEGVFIFLAPPSLSELKNRIVGRGTETEEVIANRLTVAKEEIEM
MDAYDYVVENDKVELACERIKAIVVGEHCRRERVAQYYKEMTEGI
>Mature_205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHKDTQFHYSISVTTRKPREGEIDGIDYFFKEREEFEEMIRNDQLLEWAEFVG
NYYGTPIDYVEKTLSEGKDVFLEIEVQGAMQVKKAFPEGVFIFLAPPSLSELKNRIVGRGTETEEVIANRLTVAKEEIEM
MDAYDYVVENDKVELACERIKAIVVGEHCRRERVAQYYKEMTEGI

Specific function: Essential for recycling GMP and indirectly, cGMP [H]

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain [H]

Homologues:

Organism=Homo sapiens, GI226874831, Length=176, Percent_Identity=37.5, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI226874833, Length=176, Percent_Identity=37.5, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI4504221, Length=176, Percent_Identity=37.5, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI14780902, Length=192, Percent_Identity=27.6041666666667, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI38570142, Length=146, Percent_Identity=31.5068493150685, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI21361598, Length=184, Percent_Identity=28.804347826087, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI262118255, Length=138, Percent_Identity=32.6086956521739, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4505237, Length=138, Percent_Identity=32.6086956521739, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118259, Length=138, Percent_Identity=32.6086956521739, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118263, Length=138, Percent_Identity=32.6086956521739, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI111154074, Length=113, Percent_Identity=36.283185840708, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI192447426, Length=181, Percent_Identity=28.7292817679558, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI186700627, Length=122, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI4557529, Length=181, Percent_Identity=28.7292817679558, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI186972120, Length=122, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI186700629, Length=122, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI52630447, Length=125, Percent_Identity=28.8, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI119943108, Length=179, Percent_Identity=28.4916201117318, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI261862237, Length=179, Percent_Identity=28.4916201117318, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI119943106, Length=179, Percent_Identity=28.4916201117318, Blast_Score=64, Evalue=7e-11,
Organism=Escherichia coli, GI1790080, Length=200, Percent_Identity=42.5, Blast_Score=162, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI193203132, Length=172, Percent_Identity=40.6976744186046, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17508835, Length=172, Percent_Identity=40.6976744186046, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71988048, Length=181, Percent_Identity=25.414364640884, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988041, Length=181, Percent_Identity=25.414364640884, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556244, Length=124, Percent_Identity=33.8709677419355, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984092, Length=118, Percent_Identity=28.8135593220339, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17557440, Length=121, Percent_Identity=28.099173553719, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=45.3551912568306, Blast_Score=164, Evalue=7e-42,
Organism=Drosophila melanogaster, GI21356141, Length=182, Percent_Identity=41.2087912087912, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161077626, Length=176, Percent_Identity=27.8409090909091, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640542, Length=176, Percent_Identity=27.8409090909091, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45554489, Length=176, Percent_Identity=27.8409090909091, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI116007136, Length=176, Percent_Identity=27.8409090909091, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45554481, Length=176, Percent_Identity=27.8409090909091, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI85724806, Length=176, Percent_Identity=27.8409090909091, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45551002, Length=127, Percent_Identity=33.8582677165354, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI85816106, Length=127, Percent_Identity=33.8582677165354, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI85816281, Length=127, Percent_Identity=33.8582677165354, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221330166, Length=154, Percent_Identity=31.1688311688312, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221330162, Length=154, Percent_Identity=31.1688311688312, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221330164, Length=154, Percent_Identity=31.1688311688312, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45554804, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641280, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281360700, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641282, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554818, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648808, Length=126, Percent_Identity=29.3650793650794, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI281362230, Length=126, Percent_Identity=29.3650793650794, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI161078487, Length=126, Percent_Identity=29.3650793650794, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665 [H]

Pfam domain/function: PF00625 Guanylate_kin [H]

EC number: =2.7.4.8 [H]

Molecular weight: Translated: 23618; Mature: 23618

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00856 GUANYLATE_KINASE_1 ; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRRGLLIVLSGPSGVGKGTVRKELFSHKDTQFHYSISVTTRKPREGEIDGIDYFFKER
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
EEFEEMIRNDQLLEWAEFVGNYYGTPIDYVEKTLSEGKDVFLEIEVQGAMQVKKAFPEGV
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCE
FIFLAPPSLSELKNRIVGRGTETEEVIANRLTVAKEEIEMMDAYDYVVENDKVELACERI
EEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAIVVGEHCRRERVAQYYKEMTEGI
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSRRGLLIVLSGPSGVGKGTVRKELFSHKDTQFHYSISVTTRKPREGEIDGIDYFFKER
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
EEFEEMIRNDQLLEWAEFVGNYYGTPIDYVEKTLSEGKDVFLEIEVQGAMQVKKAFPEGV
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCE
FIFLAPPSLSELKNRIVGRGTETEEVIANRLTVAKEEIEMMDAYDYVVENDKVELACERI
EEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAIVVGEHCRRERVAQYYKEMTEGI
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]