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The map label for this gene is tsf [H]

Identifier: 152976192

GI number: 152976192

Start: 2575905

End: 2576792

Strand: Reverse

Name: tsf [H]

Synonym: Bcer98_2478

Alternate gene names: 152976192

Gene position: 2576792-2575905 (Counterclockwise)

Preceding gene: 152976193

Following gene: 152976191

Centisome position: 63.05

GC content: 39.53

Gene sequence:

>888_bases
ATGGCAATTACTGCACAAATGGTAAAAGAACTTCGCGAAAAAACTGGCGCAGGTATGATGGACTGCAAAAAAGCTTTAAC
AGAAATGAATGGCGATATGGAGAAAGCAATTGATTTCTTACGTGAAAAAGGTATTGCTAAAGCTGCTAAAAAAGCAGACC
GCATCGCTGCTGAAGGACTAACTTATATCGAAACAAAAGGTAACGAAGCTTTAATCTTAGAATTAAACTCTGAAACTGAC
TTTGTTGCGAAAAATGAAGGTTTCCAAGCTTTAACAAAAGAATTAGCGGCTCACTTATTAGCTAACAAGCCTGCAACTGT
TGAAGAAGCGTTAGCTCAATCATATGAGAACGGTAAAACAGTAGAAGAGCACATCAACGAAGCAATCGCAAAAATTGGTG
AAAAGATTACTCTTCGTCGCTTTGAAATTGTTTCAAAAACTGATGCGGATGCATTCGGTGCTTACCTACACATGGGCGGA
CGCATCGGTGTAGTAACAGTTCTTGAAGGTACTACTGATGAAGAAGCTGCTAAAGATGTAGCAATGCACGTTGCAGCAGT
TAACCCTAAATATATCGACCGCGATGCTGTAACAGCTGAAGAAGTTGAACATGAGCGTCAAGTATTAACTCAACAAGCTC
TTAACGAAGGAAAACCAGAAAAAATCGTTGCTAAGATGGTTGAAGGTCGTCTAGGTAAATTCTTCGAAGAGATTTGCTTA
CTTGACCAAGCATTCGTTAAAAACCCTGATATGAAAGTTCGTCAGTTCGTTGAATCTAAAGGCGGAACAGTAAAAGGATT
TGTTCGCTACGCTGTTGGTGAAGGTATTGAGAAGCGTGAAGACAACTTTGCCGAAGAAGTAATGAACCAAGTAAAAGGTA
ACAACTAA

Upstream 100 bases:

>100_bases
AAACTGTTACTGCGTAACAAATTTGGAAAGGTGATAAGGGGTTCGGCCTTTTATCACCTTTTTTTAAGGTATAAATACAT
ATTTTAGGAGGATGAAAAGC

Downstream 100 bases:

>100_bases
TACAGATTAGGGAACACATTGTGTTCCCTTTTTTAAAATAAAGATGTAAGTAATTGGAGGTTCATTATGACTAAACCGAA
ATATAATCGTGTAGTATTAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAITAQMVKELREKTGAGMMDCKKALTEMNGDMEKAIDFLREKGIAKAAKKADRIAAEGLTYIETKGNEALILELNSETD
FVAKNEGFQALTKELAAHLLANKPATVEEALAQSYENGKTVEEHINEAIAKIGEKITLRRFEIVSKTDADAFGAYLHMGG
RIGVVTVLEGTTDEEAAKDVAMHVAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
LDQAFVKNPDMKVRQFVESKGGTVKGFVRYAVGEGIEKREDNFAEEVMNQVKGNN

Sequences:

>Translated_295_residues
MAITAQMVKELREKTGAGMMDCKKALTEMNGDMEKAIDFLREKGIAKAAKKADRIAAEGLTYIETKGNEALILELNSETD
FVAKNEGFQALTKELAAHLLANKPATVEEALAQSYENGKTVEEHINEAIAKIGEKITLRRFEIVSKTDADAFGAYLHMGG
RIGVVTVLEGTTDEEAAKDVAMHVAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
LDQAFVKNPDMKVRQFVESKGGTVKGFVRYAVGEGIEKREDNFAEEVMNQVKGNN
>Mature_294_residues
AITAQMVKELREKTGAGMMDCKKALTEMNGDMEKAIDFLREKGIAKAAKKADRIAAEGLTYIETKGNEALILELNSETDF
VAKNEGFQALTKELAAHLLANKPATVEEALAQSYENGKTVEEHINEAIAKIGEKITLRRFEIVSKTDADAFGAYLHMGGR
IGVVTVLEGTTDEEAAKDVAMHVAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICLL
DQAFVKNPDMKVRQFVESKGGTVKGFVRYAVGEGIEKREDNFAEEVMNQVKGNN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=303, Percent_Identity=24.7524752475248, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI291084500, Length=325, Percent_Identity=24.6153846153846, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI291084498, Length=120, Percent_Identity=35, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI291084502, Length=125, Percent_Identity=33.6, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1786366, Length=285, Percent_Identity=49.1228070175439, Blast_Score=238, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17561440, Length=301, Percent_Identity=26.2458471760797, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921466, Length=284, Percent_Identity=28.8732394366197, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32498; Mature: 32367

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITAQMVKELREKTGAGMMDCKKALTEMNGDMEKAIDFLREKGIAKAAKKADRIAAEGL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TYIETKGNEALILELNSETDFVAKNEGFQALTKELAAHLLANKPATVEEALAQSYENGKT
EEEEECCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCH
VEEHINEAIAKIGEKITLRRFEIVSKTDADAFGAYLHMGGRIGVVTVLEGTTDEEAAKDV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
AMHVAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQAFVKNPDMKVRQFVESKGGTVKGFVRYAVGEGIEKREDNFAEEVMNQVKGNN
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AITAQMVKELREKTGAGMMDCKKALTEMNGDMEKAIDFLREKGIAKAAKKADRIAAEGL
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TYIETKGNEALILELNSETDFVAKNEGFQALTKELAAHLLANKPATVEEALAQSYENGKT
EEEEECCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCH
VEEHINEAIAKIGEKITLRRFEIVSKTDADAFGAYLHMGGRIGVVTVLEGTTDEEAAKDV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
AMHVAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQAFVKNPDMKVRQFVESKGGTVKGFVRYAVGEGIEKREDNFAEEVMNQVKGNN
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA