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The map label for this gene is pnp [H]

Identifier: 152976173

GI number: 152976173

Start: 2554152

End: 2556284

Strand: Reverse

Name: pnp [H]

Synonym: Bcer98_2459

Alternate gene names: 152976173

Gene position: 2556284-2554152 (Counterclockwise)

Preceding gene: 152976174

Following gene: 152976172

Centisome position: 62.55

GC content: 39.19

Gene sequence:

>2133_bases
ATGAGTCAAGAAAAGCAAGTCTTCTCGATAGATTTAGCTGGTCGCCAGCTAACAATTGAAACAGGTCAGCTTGCAAAACA
AGCGAATGGAGCAGTATTAGTAAGGTATGGCGATACAGCAGTTCTATCTACAGCAACTGCATCAAAAGAGGCGAAGGATG
TAGATTTCTTCCCGCTTACAGTAAACTATGAAGAGCGTTTATATGCTGTCGGAAAAATCCCAGGTGGTTTTATTAAGCGT
GAAGGACGTCCAAGTGAAAAAGCAATTTTAGCAAGTCGTTTAATTGACCGACCAATTCGTCCGCTTTTCGCAGATGGTTT
CCGTAATGAAGTGCAAGTTGTCAGCATTGTAATGAGTGTTGATCAAGATTGTTCTTCTGAGATGGCAGCTATGCTTGGTT
CTTCATTGGCATTATCTATTTCAGATATTCCATTTGAAGGTCCAATTGCGGGGGCAACAGTAGGTCGTATTGACGGTGAA
TTTATTATTAACCCAACGGTAGAACAACAAGAGAAAAGTGATATTCACCTTGTTGTAGCGGGTACTAAAGACGCGATTAA
CATGGTAGAAGCAGGAGCTGATCAAGTACCTGAAGAAACAATGTTAGAAGCAATTATGTTCGGTCATGAAGAGATTAAAC
GTTTAATTGCTTTCCAAGAAGAAATTGTACAAGCTGTCGGAAAAGAAAAAATGGAAGTAAAACTGTATGAAGTGGATGCT
GAACTGGAAAAAGCTGTACGTGAAATGGCAGAGAAAGATATGCATACTGCAATTCAAGTACATGAAAAGCATGCGCGTGA
AGATGCAATTAACGAAGTAAAACAACGGGTCATTGCTCATTATGAAGAGCAAGAAGTTGAGGCGGAAGTATTAGGACAAG
TAAGTGAGATTTTATACAAAATTGTCAAAGAAGAAGTACGTCGTCTTATTACCGTTGAAAAAATTCGCCCAGATGGACGT
AAGAGTGATGAAATTCGTCCTTTAGCGTCAGAGGTTGGCATTTTACCTCGTACGCACGGTTCTGGTCTATTTACACGTGG
ACAAACACAAGCATTAAGTATTTGTACGTTAGGTGCATTAGGAGATGTGCAAATTTTAGATGGTCTTGGTGTAGAAGAAT
CGAAACGTTTTATGCATCATTACAATTTCCCTTCTTTCAGTGTTGGTGAAACAAGACCGATGCGTGGGCCAGGTCGCCGT
GAAATCGGTCATGGAGCATTAGGAGAACGTGCTCTTGAACCTGTAATTCCATCTGAAAAAGATTTCCCATACACAGTGCG
TCTTGTATCTGAAGTTTTGGAATCCAATGGTTCTACTTCACAAGCAAGTATTTGCGGTAGTACGTTAGCAATGATGGATG
CGGGTGTACCACTTAAAGCGCCAGTTGCTGGTATCGCAATGGGACTTGTGAAATCTGGTGAACATTATACAATTTTAACG
GATATTCAAGGCATGGAAGACCATTTAGGTGATATGGACTTTAAAGTAGCAGGTACAGCAAAAGGTGTAACTGCTCTACA
AATGGACATTAAAATCGATGGCCTTTCTCGTGAAATCTTAGAAGAAGCATTACAACAGGCTAAGGTTGGACGCATGCACA
TTCTAGATCATATGTTATCTGTTATTGCTGAACCTCGTAAAGAGCTATCTGAATATGCTCCGAAAATTATTACGATGACA
ATCAATCCGGATAAAATCCGTGATGTTATTGGACCAAGCGGTAAACAAATTAACAAAATTATTGAAGAAACAGGCGTGAA
GATTGATATTGAACAAGACGGTACAGTCTTTATTTCTTCAATTGATCAACAAATGAATGAGAAAGCGAAGAAAATCATCG
AAGATATCGTTCGCGAAGTACAAGTAGGAGAAATCTACTTAGGAAAAGTAAAACGGATTGAAAAATTCGGTGCATTCGTA
GAATTATTCAGCGGTAAAGATGGTCTTGTTCATATTTCTGAACTGGCTCTTGAGCGTGTAGGTAAAGTGGAAGACGTTGT
AAAAATCGGTGATGAAATTTCAGTGAAAGTAATCGAGATTGATAAACAAGGTCGTGTAAACTTATCTAGAAAAGTATTGC
TAAAAGAAGAGCAAGAAAAAGAGGTAGCAAAAGAAGAAGCGACACAAGAATAA

Upstream 100 bases:

>100_bases
TAGCTTACTTAATTTTCGGTAATACTAAAGGGAGAATATTTTGATATATAATCCATTTACATAGAAGAAGTGAATATTTT
AAATTGAGAGGGGTACTTAA

Downstream 100 bases:

>100_bases
AAATGAGGAAAAAAGCCAGATTTCTGGCTTTTTTTGTTATATAAAAAGATATACATAATATAGATTTTTATATGTAGCAA
GGTATAGTTAGTTATAATAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSQEKQVFSIDLAGRQLTIETGQLAKQANGAVLVRYGDTAVLSTATASKEAKDVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRIDGE
FIINPTVEQQEKSDIHLVVAGTKDAINMVEAGADQVPEETMLEAIMFGHEEIKRLIAFQEEIVQAVGKEKMEVKLYEVDA
ELEKAVREMAEKDMHTAIQVHEKHAREDAINEVKQRVIAHYEEQEVEAEVLGQVSEILYKIVKEEVRRLITVEKIRPDGR
KSDEIRPLASEVGILPRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRR
EIGHGALGERALEPVIPSEKDFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKSGEHYTILT
DIQGMEDHLGDMDFKVAGTAKGVTALQMDIKIDGLSREILEEALQQAKVGRMHILDHMLSVIAEPRKELSEYAPKIITMT
INPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISSIDQQMNEKAKKIIEDIVREVQVGEIYLGKVKRIEKFGAFV
ELFSGKDGLVHISELALERVGKVEDVVKIGDEISVKVIEIDKQGRVNLSRKVLLKEEQEKEVAKEEATQE

Sequences:

>Translated_710_residues
MSQEKQVFSIDLAGRQLTIETGQLAKQANGAVLVRYGDTAVLSTATASKEAKDVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRIDGE
FIINPTVEQQEKSDIHLVVAGTKDAINMVEAGADQVPEETMLEAIMFGHEEIKRLIAFQEEIVQAVGKEKMEVKLYEVDA
ELEKAVREMAEKDMHTAIQVHEKHAREDAINEVKQRVIAHYEEQEVEAEVLGQVSEILYKIVKEEVRRLITVEKIRPDGR
KSDEIRPLASEVGILPRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRR
EIGHGALGERALEPVIPSEKDFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKSGEHYTILT
DIQGMEDHLGDMDFKVAGTAKGVTALQMDIKIDGLSREILEEALQQAKVGRMHILDHMLSVIAEPRKELSEYAPKIITMT
INPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISSIDQQMNEKAKKIIEDIVREVQVGEIYLGKVKRIEKFGAFV
ELFSGKDGLVHISELALERVGKVEDVVKIGDEISVKVIEIDKQGRVNLSRKVLLKEEQEKEVAKEEATQE
>Mature_709_residues
SQEKQVFSIDLAGRQLTIETGQLAKQANGAVLVRYGDTAVLSTATASKEAKDVDFFPLTVNYEERLYAVGKIPGGFIKRE
GRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRIDGEF
IINPTVEQQEKSDIHLVVAGTKDAINMVEAGADQVPEETMLEAIMFGHEEIKRLIAFQEEIVQAVGKEKMEVKLYEVDAE
LEKAVREMAEKDMHTAIQVHEKHAREDAINEVKQRVIAHYEEQEVEAEVLGQVSEILYKIVKEEVRRLITVEKIRPDGRK
SDEIRPLASEVGILPRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRRE
IGHGALGERALEPVIPSEKDFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKSGEHYTILTD
IQGMEDHLGDMDFKVAGTAKGVTALQMDIKIDGLSREILEEALQQAKVGRMHILDHMLSVIAEPRKELSEYAPKIITMTI
NPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISSIDQQMNEKAKKIIEDIVREVQVGEIYLGKVKRIEKFGAFVE
LFSGKDGLVHISELALERVGKVEDVVKIGDEISVKVIEIDKQGRVNLSRKVLLKEEQEKEVAKEEATQE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=37.1468926553672, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI9506689, Length=226, Percent_Identity=25.6637168141593, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=50.9489051094891, Blast_Score=672, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=716, Percent_Identity=34.2178770949721, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=40.5099150141643, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=40.5099150141643, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=40.5099150141643, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI161079377, Length=682, Percent_Identity=39.1495601173021, Blast_Score=438, Evalue=1e-123,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78220; Mature: 78089

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKQVFSIDLAGRQLTIETGQLAKQANGAVLVRYGDTAVLSTATASKEAKDVDFFPLT
CCCCCEEEEEEECCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
DQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRIDGEFIINPTVEQQEKSDIHLVVA
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEEEE
GTKDAINMVEAGADQVPEETMLEAIMFGHEEIKRLIAFQEEIVQAVGKEKMEVKLYEVDA
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCH
ELEKAVREMAEKDMHTAIQVHEKHAREDAINEVKQRVIAHYEEQEVEAEVLGQVSEILYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKEEVRRLITVEKIRPDGRKSDEIRPLASEVGILPRTHGSGLFTRGQTQALSICTLGAL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCCEEEEEEECCC
GDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRREIGHGALGERALEPVIPSEK
CCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
DFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKSGEHYTILT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DIQGMEDHLGDMDFKVAGTAKGVTALQMDIKIDGLSREILEEALQQAKVGRMHILDHMLS
ECCCHHHHCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHH
VIAEPRKELSEYAPKIITMTINPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISS
HHHHHHHHHHHHCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEHHH
IDQQMNEKAKKIIEDIVREVQVGEIYLGKVKRIEKFGAFVELFSGKDGLVHISELALERV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
GKVEDVVKIGDEISVKVIEIDKQGRVNLSRKVLLKEEQEKEVAKEEATQE
CCHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQEKQVFSIDLAGRQLTIETGQLAKQANGAVLVRYGDTAVLSTATASKEAKDVDFFPLT
CCCCEEEEEEECCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
DQDCSSEMAAMLGSSLALSISDIPFEGPIAGATVGRIDGEFIINPTVEQQEKSDIHLVVA
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEEEE
GTKDAINMVEAGADQVPEETMLEAIMFGHEEIKRLIAFQEEIVQAVGKEKMEVKLYEVDA
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCH
ELEKAVREMAEKDMHTAIQVHEKHAREDAINEVKQRVIAHYEEQEVEAEVLGQVSEILYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKEEVRRLITVEKIRPDGRKSDEIRPLASEVGILPRTHGSGLFTRGQTQALSICTLGAL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCCEEEEEEECCC
GDVQILDGLGVEESKRFMHHYNFPSFSVGETRPMRGPGRREIGHGALGERALEPVIPSEK
CCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
DFPYTVRLVSEVLESNGSTSQASICGSTLAMMDAGVPLKAPVAGIAMGLVKSGEHYTILT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DIQGMEDHLGDMDFKVAGTAKGVTALQMDIKIDGLSREILEEALQQAKVGRMHILDHMLS
ECCCHHHHCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHH
VIAEPRKELSEYAPKIITMTINPDKIRDVIGPSGKQINKIIEETGVKIDIEQDGTVFISS
HHHHHHHHHHHHCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEHHH
IDQQMNEKAKKIIEDIVREVQVGEIYLGKVKRIEKFGAFVELFSGKDGLVHISELALERV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
GKVEDVVKIGDEISVKVIEIDKQGRVNLSRKVLLKEEQEKEVAKEEATQE
CCHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA