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The map label for this gene is rnjB [H]

Identifier: 152976163

GI number: 152976163

Start: 2543665

End: 2545416

Strand: Reverse

Name: rnjB [H]

Synonym: Bcer98_2449

Alternate gene names: 152976163

Gene position: 2545416-2543665 (Counterclockwise)

Preceding gene: 152976165

Following gene: 152976162

Centisome position: 62.28

GC content: 36.82

Gene sequence:

>1752_bases
ATGGTGGTTGTAGAGTTTCCCTGGTGTCTCTACAATTTTAGTGAAATCAGTGTTTACAAGATTTTTAAACCAGGCAACAA
CATGAAGAGAAAAGAGATTGATTCTGTAAAGGTATTTGCTCTTGGTGGAGTAGGTGAAATCGGAAAAAATATGTACTGCG
TCGAAATTGATTCCGAAATTTTTATCGTCGATGCAGGCTTAATGTTTCCAGGAGATGAGATGTTTGGAATTGATATCGTA
ATCCCTGATATTACATATTTAATAGAAAATCAAGAGCGAGTAAAAGGTTTATTTATTACACATGGTCATGAAGACCATAT
TGGTGGAATTGTTTATGTAATGCGTAAGCTTTCCATTCCGGTATATGCAACCAAGTTAACGGTAGGATTAATTCAAGAAA
AGCTGAATGAAGCAGGAATGCTTGGTCGTGTAGACTTAAAAACAATTGATTCAAATTCGGAAGTTGAATTTAATACGACA
ACGGTTTCATTCTTTGGAACAACGCACAGTATTCCTGATTCTGTTGGTGTATGCTTCCATACATCAAAAGGTGCGATTGT
ATATACAGGAGATTTTAAATTTGATCAAACACCGATTGGTAATAGCGGAGCAGATCTTGGAAAGATGGCAAAAATCGGAA
ATGAAGGTGTGCTCTGCTTATTATCTGATAGTACGAATGCGGAACGACCTGGTTACACAGGTTCTGAAAAAGAAGTTGGT
ATGGAAATATCAAAAGTTTTTTATGGCGCAGAAGGACGCATTATTGTTGCTTCTTTTGCATCCAATGTTCACCGTATTCA
GCAAGTTTTTGATGCAGCGGCTGAAACAGGACGTAAAGTAGCGGTTGTTGGTCGTAGCATGGTGAAAGTAGTAGATATTG
CAAGACGTCTTGGGTATTTAGATGTGCCGGATGGCATGCTTATTTCATTGCAAGAAGTAGAGAACTTCCCGGAGAAGAAA
GTGGCAATTTTAACAACAGGTAGCCAAGGTGAACCGATGGCAGCCCTTTCAAGAATGGCAAAGCAATCTCATAAACAAAT
TTCCATTCGCAAAGGAGATACAGTTATTATTGCAGCATCTCCAATTCCTGGTAATGAAGTGTCGGTATCTAAAATTATCG
ATTTATTATTTAGAGCCGGTGCGGAAGTTGTCTATTATGGTGAAAGAAAAGTACACGTTTCTGGTCATGGTAGTCAAGAA
GAATTAAAATTAATGTTAAATTTAATGAAACCGCAATATTTTATACCCGTACATGGTGAATTCCGTATGCAAAAAGCACA
TGCGTATTTAGCTGAAGATGTAGGTATTGCAAGAGAAAATATTTTCATTGTCGATAAGGGTGATGTAATTGCTTTTGAAG
GTGATGAGGCAAAACCAGCTGGCAAAGTACAAGCAGGTAATGTTCTTATTGATGGATTAGGTGTAGGAGATGTTGGGAAC
ATCGTTCTGCGCGATCGAAAAATGTTATCACAAGATGGTATTTTAGTTGTTGTAGTAACGCTTGGAAAAGAAGAGAAAAA
AATAATCTCTGGTCCAGAAATTATTTCACGCGGATTTGTCTATGTTCGTGAATCAGAGGCATTAATTGAAAGATCTACAG
ACATTGTACGTACAATTGTAGAACAGTCTATTAAAGAATATTCGATTGAATGGTCTATGTTGAAGCAAAACATTCGTGAA
CTATTAGGACAATTCTTATATGAAGAAACGAAAAGAAAACCAATGATTTTACCGATTATTATGGAAGTGTAA

Upstream 100 bases:

>100_bases
TGATTTATATAAATGAAAGAGAGGTGAAACCTGGTTTAAAAAATGAAAAAGAACATGATATCGCATAACGGTAAGGACAT
TCGTCTTGGACGGTGAATAT

Downstream 100 bases:

>100_bases
AAGGTCACCTTTACTGGTGACCTTTTTTCTTTTCATCCCTCTCGAATGAAATTTACAACTTAAGAAATAATAGGTGTATA
CGAGCGAAAGGGGATGCGAT

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MVVVEFPWCLYNFSEISVYKIFKPGNNMKRKEIDSVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIV
IPDITYLIENQERVKGLFITHGHEDHIGGIVYVMRKLSIPVYATKLTVGLIQEKLNEAGMLGRVDLKTIDSNSEVEFNTT
TVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLGKMAKIGNEGVLCLLSDSTNAERPGYTGSEKEVG
MEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPDGMLISLQEVENFPEKK
VAILTTGSQGEPMAALSRMAKQSHKQISIRKGDTVIIAASPIPGNEVSVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQE
ELKLMLNLMKPQYFIPVHGEFRMQKAHAYLAEDVGIARENIFIVDKGDVIAFEGDEAKPAGKVQAGNVLIDGLGVGDVGN
IVLRDRKMLSQDGILVVVVTLGKEEKKIISGPEIISRGFVYVRESEALIERSTDIVRTIVEQSIKEYSIEWSMLKQNIRE
LLGQFLYEETKRKPMILPIIMEV

Sequences:

>Translated_583_residues
MVVVEFPWCLYNFSEISVYKIFKPGNNMKRKEIDSVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIV
IPDITYLIENQERVKGLFITHGHEDHIGGIVYVMRKLSIPVYATKLTVGLIQEKLNEAGMLGRVDLKTIDSNSEVEFNTT
TVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLGKMAKIGNEGVLCLLSDSTNAERPGYTGSEKEVG
MEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPDGMLISLQEVENFPEKK
VAILTTGSQGEPMAALSRMAKQSHKQISIRKGDTVIIAASPIPGNEVSVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQE
ELKLMLNLMKPQYFIPVHGEFRMQKAHAYLAEDVGIARENIFIVDKGDVIAFEGDEAKPAGKVQAGNVLIDGLGVGDVGN
IVLRDRKMLSQDGILVVVVTLGKEEKKIISGPEIISRGFVYVRESEALIERSTDIVRTIVEQSIKEYSIEWSMLKQNIRE
LLGQFLYEETKRKPMILPIIMEV
>Mature_583_residues
MVVVEFPWCLYNFSEISVYKIFKPGNNMKRKEIDSVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIV
IPDITYLIENQERVKGLFITHGHEDHIGGIVYVMRKLSIPVYATKLTVGLIQEKLNEAGMLGRVDLKTIDSNSEVEFNTT
TVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLGKMAKIGNEGVLCLLSDSTNAERPGYTGSEKEVG
MEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPDGMLISLQEVENFPEKK
VAILTTGSQGEPMAALSRMAKQSHKQISIRKGDTVIIAASPIPGNEVSVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQE
ELKLMLNLMKPQYFIPVHGEFRMQKAHAYLAEDVGIARENIFIVDKGDVIAFEGDEAKPAGKVQAGNVLIDGLGVGDVGN
IVLRDRKMLSQDGILVVVVTLGKEEKKIISGPEIISRGFVYVRESEALIERSTDIVRTIVEQSIKEYSIEWSMLKQNIRE
LLGQFLYEETKRKPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 64259; Mature: 64259

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVVEFPWCLYNFSEISVYKIFKPGNNMKRKEIDSVKVFALGGVGEIGKNMYCVEIDSEI
CEEEECCHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCEEEEEECCEE
FIVDAGLMFPGDEMFGIDIVIPDITYLIENQERVKGLFITHGHEDHIGGIVYVMRKLSIP
EEEEECEECCCCCEEEEEEEECCEEEEECCCHHEEEEEEECCCCCCCCHHHHHHHHHCCC
VYATKLTVGLIQEKLNEAGMLGRVDLKTIDSNSEVEFNTTTVSFFGTTHSIPDSVGVCFH
EEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEEEE
TSKGAIVYTGDFKFDQTPIGNSGADLGKMAKIGNEGVLCLLSDSTNAERPGYTGSEKEVG
CCCCEEEEECCCEECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHC
MEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYL
CEEEHEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCE
DVPDGMLISLQEVENFPEKKVAILTTGSQGEPMAALSRMAKQSHKQISIRKGDTVIIAAS
ECCCCCEEEHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCEEEEEEC
PIPGNEVSVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPQYFIPVHGE
CCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCC
FRMQKAHAYLAEDVGIARENIFIVDKGDVIAFEGDEAKPAGKVQAGNVLIDGLGVGDVGN
EEEHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCEEECCEEEEECCCCCCCC
IVLRDRKMLSQDGILVVVVTLGKEEKKIISGPEIISRGFVYVRESEALIERSTDIVRTIV
EEEECHHHHCCCCEEEEEEECCCCCHHHCCCCHHHHCCEEEEECCHHHHHHHHHHHHHHH
EQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MVVVEFPWCLYNFSEISVYKIFKPGNNMKRKEIDSVKVFALGGVGEIGKNMYCVEIDSEI
CEEEECCHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCEEEEEECCEE
FIVDAGLMFPGDEMFGIDIVIPDITYLIENQERVKGLFITHGHEDHIGGIVYVMRKLSIP
EEEEECEECCCCCEEEEEEEECCEEEEECCCHHEEEEEEECCCCCCCCHHHHHHHHHCCC
VYATKLTVGLIQEKLNEAGMLGRVDLKTIDSNSEVEFNTTTVSFFGTTHSIPDSVGVCFH
EEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEEEE
TSKGAIVYTGDFKFDQTPIGNSGADLGKMAKIGNEGVLCLLSDSTNAERPGYTGSEKEVG
CCCCEEEEECCCEECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHC
MEISKVFYGAEGRIIVASFASNVHRIQQVFDAAAETGRKVAVVGRSMVKVVDIARRLGYL
CEEEHEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCE
DVPDGMLISLQEVENFPEKKVAILTTGSQGEPMAALSRMAKQSHKQISIRKGDTVIIAAS
ECCCCCEEEHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCEEEEEEC
PIPGNEVSVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPQYFIPVHGE
CCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCC
FRMQKAHAYLAEDVGIARENIFIVDKGDVIAFEGDEAKPAGKVQAGNVLIDGLGVGDVGN
EEEHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCEEECCEEEEECCCCCCCC
IVLRDRKMLSQDGILVVVVTLGKEEKKIISGPEIISRGFVYVRESEALIERSTDIVRTIV
EEEECHHHHCCCCEEEEEEECCCCCHHHCCCCHHHHCCEEEEECCHHHHHHHHHHHHHHH
EQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]