| Definition | Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome. |
|---|---|
| Accession | NC_009674 |
| Length | 4,087,024 |
Click here to switch to the map view.
The map label for this gene is cwlH [H]
Identifier: 152976053
GI number: 152976053
Start: 2422538
End: 2424328
Strand: Reverse
Name: cwlH [H]
Synonym: Bcer98_2329
Alternate gene names: 152976053
Gene position: 2424328-2422538 (Counterclockwise)
Preceding gene: 152976054
Following gene: 152976052
Centisome position: 59.32
GC content: 36.63
Gene sequence:
>1791_bases ATGAGAAAAGGAGGCGTAAGAACACGAAATGTTGTTGGACATAACGTGTTAAGAAGTAGAATGAAAAAAGGACTTTATAA TGTTTTCGCAATGTCAATTGCATTGCAGGTGGTAATGATTCCAAGTGATTCTTTTGCAGCACCGTTAGAAAAAACGATAG AGTTATCAACAGATGAGCAAGCGTTGAAAAGTATTGAGAATCATATGAAACATGAAGATGGACGTGGTGATAGTCAAGAA GCTAATAGTGAAGTACAAGGGGATTTTCTGGTACATATAAAAAAAGAAACGTCAATATATGAATCATCGGACTTAAAGGA ATGGACTGGGAAGAATTTTTCTAAGCAAGTGGTGAAAGCTTCAAAGAGAAAAGGAAATGCTTATTACATTCAAACACGAA ATGAAGCAGGGTGGATACAACATATAGATAATCATGTTGAAGTTCGAAATATTTATCCATTATCGAATAAGAGACTCGTT ATTCATGAAGAAACTGCTCTGTATTCTTCACCTTTCGATTCATTTCAAGAAGAAAGTACATTACAACCTCAAACAGTTGT AGCGATTGGACAAGCGGGAGATTGGTTCCAAGTTGAAACAAATGAAGGGAAAAAGTGGATTCATTCCTCAAATGTAGCGT TTGAAGGGACAAAAGCATCCCTTATTCAAAATAACGAGATGCCAACTTATAAAAAACGTGAGGCAGGTCTATATTATGCA GCACCGTCTGCTTTAAAAATAGGATCTGTTTATGGGGTACCATTTACAGAATGGATTGTCCCAAAAGGAAATGATAATAT TCGTCCTGGTTATGCAATGGATCCAAAGTATATTACCATTCATGAAACGGATAATTATAGCGTTGGTGCAGGGGCTAGAA ATCATGCTCAATATTTATATAATCAAGCAACAGGGGATACAGATCGCTCAGCATCTTGGCATTTTACAGTGGATGATAAA GAGATTTATCAGCATCTTCCGTTAAATGAAAATGGTTGGCATGCAGGAGATGGATCCAATGGAACAGGTAATAGACAATC CATTGCGATTGAGATTGCTGTAAATCAAGATGGGAATTACGATAAAGCGGTAGAAAATGCTCGGAAATTAACAGCTTATT TGATGGGAGAGTTACACATTCCTATAGAAAATGTGAAAAAACACCAATATTGGAGTGGGAAAATTTGTCCCGCAATTATG ATTCAACGCGGTGCGTGGAATGCTTTCTTACAAGGTGCAAAAAGTTACTATGATGAAAATCATCAAGATGATATTACAGG TGGATGGTATGAACAAGATATTCGCGAATTAGCTGCGAAAGGAATTATGGTTGGGGATGGAAATGGTTCCTATTGGCCAA ATCGTCTTGTGACGCGCGGAGAATTTGCAAATTTCATTTCGCGATCATTACAGCTGCCAGAAGGAAGTTCAACGTTTACA GATTTAAATGAAGCTCATCCATCATTAGTAGACGGTATTAATCGTGCAGCAAGTGCAGGAATTATTAATGGTCGAGGAGA CAATACGTTTGATCCAAATGATACAATTAAACGTGATGAAGTTGTCATTATGATTGATCGTGCTTTACAGTATAAAGGTA TCACAGGAACTTTAGTTGAAGTACCATTTTCTGATCAAAACTTAGCATATGACAAGCAAGCATTAAAACGTGTCTATGGA TTAGGAATTGTAAAAGGAAATGAAAATAATGAATTTATGCCAAAAGGAACAGCTACTCGCGGCGAATCAGCAGCATTTAT AAATAGAATGTTATATGTAATAGAGAGGTAA
Upstream 100 bases:
>100_bases TAACGATAAATGATGTCGAATCTTATCTATACGAATAAGAAAAAAGTCGTTATAATTTAAGGTAGTTAAGTAATTTAGTA GGTTGCTAAAATATAATTGT
Downstream 100 bases:
>100_bases TAAGCAAAAAGTACTCACGTTTGTGGGTACTTTTTCTTTTTCAATATATCTGAAACAGGAATTGCATATTTTGTATGGAA TAAGGAATAAAAGTGGAGTG
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: Autolysin; Cell wall hydrolase [H]
Number of amino acids: Translated: 596; Mature: 596
Protein sequence:
>596_residues MRKGGVRTRNVVGHNVLRSRMKKGLYNVFAMSIALQVVMIPSDSFAAPLEKTIELSTDEQALKSIENHMKHEDGRGDSQE ANSEVQGDFLVHIKKETSIYESSDLKEWTGKNFSKQVVKASKRKGNAYYIQTRNEAGWIQHIDNHVEVRNIYPLSNKRLV IHEETALYSSPFDSFQEESTLQPQTVVAIGQAGDWFQVETNEGKKWIHSSNVAFEGTKASLIQNNEMPTYKKREAGLYYA APSALKIGSVYGVPFTEWIVPKGNDNIRPGYAMDPKYITIHETDNYSVGAGARNHAQYLYNQATGDTDRSASWHFTVDDK EIYQHLPLNENGWHAGDGSNGTGNRQSIAIEIAVNQDGNYDKAVENARKLTAYLMGELHIPIENVKKHQYWSGKICPAIM IQRGAWNAFLQGAKSYYDENHQDDITGGWYEQDIRELAAKGIMVGDGNGSYWPNRLVTRGEFANFISRSLQLPEGSSTFT DLNEAHPSLVDGINRAASAGIINGRGDNTFDPNDTIKRDEVVIMIDRALQYKGITGTLVEVPFSDQNLAYDKQALKRVYG LGIVKGNENNEFMPKGTATRGESAAFINRMLYVIER
Sequences:
>Translated_596_residues MRKGGVRTRNVVGHNVLRSRMKKGLYNVFAMSIALQVVMIPSDSFAAPLEKTIELSTDEQALKSIENHMKHEDGRGDSQE ANSEVQGDFLVHIKKETSIYESSDLKEWTGKNFSKQVVKASKRKGNAYYIQTRNEAGWIQHIDNHVEVRNIYPLSNKRLV IHEETALYSSPFDSFQEESTLQPQTVVAIGQAGDWFQVETNEGKKWIHSSNVAFEGTKASLIQNNEMPTYKKREAGLYYA APSALKIGSVYGVPFTEWIVPKGNDNIRPGYAMDPKYITIHETDNYSVGAGARNHAQYLYNQATGDTDRSASWHFTVDDK EIYQHLPLNENGWHAGDGSNGTGNRQSIAIEIAVNQDGNYDKAVENARKLTAYLMGELHIPIENVKKHQYWSGKICPAIM IQRGAWNAFLQGAKSYYDENHQDDITGGWYEQDIRELAAKGIMVGDGNGSYWPNRLVTRGEFANFISRSLQLPEGSSTFT DLNEAHPSLVDGINRAASAGIINGRGDNTFDPNDTIKRDEVVIMIDRALQYKGITGTLVEVPFSDQNLAYDKQALKRVYG LGIVKGNENNEFMPKGTATRGESAAFINRMLYVIER >Mature_596_residues MRKGGVRTRNVVGHNVLRSRMKKGLYNVFAMSIALQVVMIPSDSFAAPLEKTIELSTDEQALKSIENHMKHEDGRGDSQE ANSEVQGDFLVHIKKETSIYESSDLKEWTGKNFSKQVVKASKRKGNAYYIQTRNEAGWIQHIDNHVEVRNIYPLSNKRLV IHEETALYSSPFDSFQEESTLQPQTVVAIGQAGDWFQVETNEGKKWIHSSNVAFEGTKASLIQNNEMPTYKKREAGLYYA APSALKIGSVYGVPFTEWIVPKGNDNIRPGYAMDPKYITIHETDNYSVGAGARNHAQYLYNQATGDTDRSASWHFTVDDK EIYQHLPLNENGWHAGDGSNGTGNRQSIAIEIAVNQDGNYDKAVENARKLTAYLMGELHIPIENVKKHQYWSGKICPAIM IQRGAWNAFLQGAKSYYDENHQDDITGGWYEQDIRELAAKGIMVGDGNGSYWPNRLVTRGEFANFISRSLQLPEGSSTFT DLNEAHPSLVDGINRAASAGIINGRGDNTFDPNDTIKRDEVVIMIDRALQYKGITGTLVEVPFSDQNLAYDKQALKRVYG LGIVKGNENNEFMPKGTATRGESAAFINRMLYVIER
Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]
COG id: COG5632
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Secreted (Potential) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002502 - InterPro: IPR002477 [H]
Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 66782; Mature: 66782
Theoretical pI: Translated: 6.57; Mature: 6.57
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRKGGVRTRNVVGHNVLRSRMKKGLYNVFAMSIALQVVMIPSDSFAAPLEKTIELSTDEQ CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHEECCCHHH ALKSIENHMKHEDGRGDSQEANSEVQGDFLVHIKKETSIYESSDLKEWTGKNFSKQVVKA HHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCHHHHHHHH SKRKGNAYYIQTRNEAGWIQHIDNHVEVRNIYPLSNKRLVIHEETALYSSPFDSFQEEST HHCCCCEEEEEECCCCCHHHHCCCCEEEEEEEECCCCEEEEEECCHHHCCCCHHHHHHCC LQPQTVVAIGQAGDWFQVETNEGKKWIHSSNVAFEGTKASLIQNNEMPTYKKREAGLYYA CCCCEEEEECCCCCEEEEECCCCCEEEECCCCEECCCHHHEEECCCCCCCHHHHCCEEEE APSALKIGSVYGVPFTEWIVPKGNDNIRPGYAMDPKYITIHETDNYSVGAGARNHAQYLY CCCCEEECCEECCCHHHEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHH NQATGDTDRSASWHFTVDDKEIYQHLPLNENGWHAGDGSNGTGNRQSIAIEIAVNQDGNY HCCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCH DKAVENARKLTAYLMGELHIPIENVKKHQYWSGKICPAIMIQRGAWNAFLQGAKSYYDEN HHHHHHHHHHHHHHHCEEECCHHHHHHCCCCCCCCCHHHEEECCCHHHHHHHHHHHCCCC HQDDITGGWYEQDIRELAAKGIMVGDGNGSYWPNRLVTRGEFANFISRSLQLPEGSSTFT CCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHEECHHHHHHHHHHCCCCCCCCCCC DLNEAHPSLVDGINRAASAGIINGRGDNTFDPNDTIKRDEVVIMIDRALQYKGITGTLVE CHHHCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEE VPFSDQNLAYDKQALKRVYGLGIVKGNENNEFMPKGTATRGESAAFINRMLYVIER CCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC >Mature Secondary Structure MRKGGVRTRNVVGHNVLRSRMKKGLYNVFAMSIALQVVMIPSDSFAAPLEKTIELSTDEQ CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHEECCCHHH ALKSIENHMKHEDGRGDSQEANSEVQGDFLVHIKKETSIYESSDLKEWTGKNFSKQVVKA HHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCHHHHHHHH SKRKGNAYYIQTRNEAGWIQHIDNHVEVRNIYPLSNKRLVIHEETALYSSPFDSFQEEST HHCCCCEEEEEECCCCCHHHHCCCCEEEEEEEECCCCEEEEEECCHHHCCCCHHHHHHCC LQPQTVVAIGQAGDWFQVETNEGKKWIHSSNVAFEGTKASLIQNNEMPTYKKREAGLYYA CCCCEEEEECCCCCEEEEECCCCCEEEECCCCEECCCHHHEEECCCCCCCHHHHCCEEEE APSALKIGSVYGVPFTEWIVPKGNDNIRPGYAMDPKYITIHETDNYSVGAGARNHAQYLY CCCCEEECCEECCCHHHEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHH NQATGDTDRSASWHFTVDDKEIYQHLPLNENGWHAGDGSNGTGNRQSIAIEIAVNQDGNY HCCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCH DKAVENARKLTAYLMGELHIPIENVKKHQYWSGKICPAIMIQRGAWNAFLQGAKSYYDEN HHHHHHHHHHHHHHHCEEECCHHHHHHCCCCCCCCCHHHEEECCCHHHHHHHHHHHCCCC HQDDITGGWYEQDIRELAAKGIMVGDGNGSYWPNRLVTRGEFANFISRSLQLPEGSSTFT CCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHEECHHHHHHHHHHCCCCCCCCCCC DLNEAHPSLVDGINRAASAGIINGRGDNTFDPNDTIKRDEVVIMIDRALQYKGITGTLVE CHHHCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEE VPFSDQNLAYDKQALKRVYGLGIVKGNENNEFMPKGTATRGESAAFINRMLYVIER CCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8969508; 9384377 [H]