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The map label for this gene is nodB [H]

Identifier: 152975993

GI number: 152975993

Start: 2366708

End: 2367433

Strand: Reverse

Name: nodB [H]

Synonym: Bcer98_2266

Alternate gene names: 152975993

Gene position: 2367433-2366708 (Counterclockwise)

Preceding gene: 152975994

Following gene: 152975991

Centisome position: 57.93

GC content: 37.47

Gene sequence:

>726_bases
ATGTTCAGACGAAAAACTGCCAATATTATTATTTGTTTTATTAGTTTTATACATATATGTCAAGTGGACGAAGTACAAGC
AAGTTTGTTATTAAGAAAAGAGTTGGAACCTACTGGCTATGTAACGTGGGAAGTTCCAAATAAAGAGAAGATTATCGCTA
TTACATTTGATGATGGACCAGATCCGATATATACGCCGCAAGTATTACAATTACTGCGTCAATACAAAGCGGAAGCAACT
TTTTTTATGATTGGATTTCGAATTCAACGAAACCCTTATTTAGTGAAGCAAGTATTAAAAGAAGGACATGAAATTGGTAA
TCATACAATGAATCACTTATACGCAAAAAGAACGCAGAGTGAAAAAGTAAAAAATGATATTTTAGAGGGAAAGAAGTACT
TAGCGAAGTGGATAAAAGAACCTCTCCTCTTTCGTCCACCTGGTGGGGAAATAAATGATACCGTCTTTACTACCGCAAAA
GAAGCAGGATATCAAATTGTATTATGGTCATGGCATCAAGATCCGCGTGATTGGGCGAATCCAGGTACCGCATCTATTGT
GAACCATGTTTTGAAAAATGCGAAGAGTGGAGATATTGTCCTGTTACATGATGGGGGGAGTGACCGTAGCCAAACAATAG
AAGCGCTGGCGAAGATCTTACCAGAGCTTCAACAAAGAGGATATCGCTTTGTGAAGGTATCGGAACTATTGCGTTATAAG
CATTAA

Upstream 100 bases:

>100_bases
CAATATGAAAGTCATTTGCAAAATATTATGCGAGAAGTGGATCAGTTAGAGAAGTGAAGAGGTTGTTGTCGTTTCAGCAA
CCTCTCTATTAGGAACACAT

Downstream 100 bases:

>100_bases
AAAGCTCCCAAAAGGATTATCTTTTGGGAGTTTTCGTTACTGATTTTTTTTCCTCTTTTTATTATTTTGACGCTGAGCAG
CTGTTAATGGTTCATTTGCG

Product: sporulation polysaccharide deacetylase PdaB

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MFRRKTANIIICFISFIHICQVDEVQASLLLRKELEPTGYVTWEVPNKEKIIAITFDDGPDPIYTPQVLQLLRQYKAEAT
FFMIGFRIQRNPYLVKQVLKEGHEIGNHTMNHLYAKRTQSEKVKNDILEGKKYLAKWIKEPLLFRPPGGEINDTVFTTAK
EAGYQIVLWSWHQDPRDWANPGTASIVNHVLKNAKSGDIVLLHDGGSDRSQTIEALAKILPELQQRGYRFVKVSELLRYK
H

Sequences:

>Translated_241_residues
MFRRKTANIIICFISFIHICQVDEVQASLLLRKELEPTGYVTWEVPNKEKIIAITFDDGPDPIYTPQVLQLLRQYKAEAT
FFMIGFRIQRNPYLVKQVLKEGHEIGNHTMNHLYAKRTQSEKVKNDILEGKKYLAKWIKEPLLFRPPGGEINDTVFTTAK
EAGYQIVLWSWHQDPRDWANPGTASIVNHVLKNAKSGDIVLLHDGGSDRSQTIEALAKILPELQQRGYRFVKVSELLRYK
H
>Mature_241_residues
MFRRKTANIIICFISFIHICQVDEVQASLLLRKELEPTGYVTWEVPNKEKIIAITFDDGPDPIYTPQVLQLLRQYKAEAT
FFMIGFRIQRNPYLVKQVLKEGHEIGNHTMNHLYAKRTQSEKVKNDILEGKKYLAKWIKEPLLFRPPGGEINDTVFTTAK
EAGYQIVLWSWHQDPRDWANPGTASIVNHVLKNAKSGDIVLLHDGGSDRSQTIEALAKILPELQQRGYRFVKVSELLRYK
H

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=176, Percent_Identity=32.3863636363636, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323338, Length=132, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 27843; Mature: 27843

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRKTANIIICFISFIHICQVDEVQASLLLRKELEPTGYVTWEVPNKEKIIAITFDDGP
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCC
DPIYTPQVLQLLRQYKAEATFFMIGFRIQRNPYLVKQVLKEGHEIGNHTMNHLYAKRTQS
CCCCCHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVKNDILEGKKYLAKWIKEPLLFRPPGGEINDTVFTTAKEAGYQIVLWSWHQDPRDWAN
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEHHHCCCEEEEEECCCCCHHHCC
PGTASIVNHVLKNAKSGDIVLLHDGGSDRSQTIEALAKILPELQQRGYRFVKVSELLRYK
CCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCC
H
C
>Mature Secondary Structure
MFRRKTANIIICFISFIHICQVDEVQASLLLRKELEPTGYVTWEVPNKEKIIAITFDDGP
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCC
DPIYTPQVLQLLRQYKAEATFFMIGFRIQRNPYLVKQVLKEGHEIGNHTMNHLYAKRTQS
CCCCCHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVKNDILEGKKYLAKWIKEPLLFRPPGGEINDTVFTTAKEAGYQIVLWSWHQDPRDWAN
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEHHHCCCEEEEEECCCCCHHHCC
PGTASIVNHVLKNAKSGDIVLLHDGGSDRSQTIEALAKILPELQQRGYRFVKVSELLRYK
CCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3960737 [H]