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The map label for this gene is cspB [H]

Identifier: 152975949

GI number: 152975949

Start: 2310359

End: 2310559

Strand: Reverse

Name: cspB [H]

Synonym: Bcer98_2221

Alternate gene names: 152975949

Gene position: 2310559-2310359 (Counterclockwise)

Preceding gene: 152975950

Following gene: 152975945

Centisome position: 56.53

GC content: 39.8

Gene sequence:

>201_bases
ATGCAAAACGGTAAAGTAAAATGGTTTAACGCAGAAAAAGGTTTCGGATTCATCGAAGTTGAAGGCGGAGAAGACGTATT
CGTTCATTTCTCAGCAATCCAAGGCGAAGGATTCAAAACATTAGAAGAAGGTCAAGAAGTTACTTTCGAAGTTGAACAAG
GTAACCGCGGACCACAAGCTACTAACGTAGTTAAGAAGTAA

Upstream 100 bases:

>100_bases
AATCTCCTTTGTGGTTACAGTTTTTTACTGTCTTCGTTATTTTTGTAAAGTGGTTGAAAATCAATATGTACATATAGGTA
CATTGTAGGAGGATTTTTTC

Downstream 100 bases:

>100_bases
TTGAACTAAAAAGAGAAGGGATTCCCCTTCTCTTTTTTTGCATTTGTTAGCTTATTTTCTCATTTATAATAGAGAAAAGT
ACGATACTTTAATTGCGAGC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MQNGKVKWFNAEKGFGFIEVEGGEDVFVHFSAIQGEGFKTLEEGQEVTFEVEQGNRGPQATNVVKK

Sequences:

>Translated_66_residues
MQNGKVKWFNAEKGFGFIEVEGGEDVFVHFSAIQGEGFKTLEEGQEVTFEVEQGNRGPQATNVVKK
>Mature_66_residues
MQNGKVKWFNAEKGFGFIEVEGGEDVFVHFSAIQGEGFKTLEEGQEVTFEVEQGNRGPQATNVVKK

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787839, Length=64, Percent_Identity=62.5, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=60.9375, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=66.1290322580645, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=64.5161290322581, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=66.1290322580645, Blast_Score=82, Evalue=7e-18,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=62.5, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1787107, Length=65, Percent_Identity=55.3846153846154, Blast_Score=75, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24658520, Length=64, Percent_Identity=50, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7335; Mature: 7335

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNGKVKWFNAEKGFGFIEVEGGEDVFVHFSAIQGEGFKTLEEGQEVTFEVEQGNRGPQA
CCCCCEEEEECCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCC
TNVVKK
CCCCCC
>Mature Secondary Structure
MQNGKVKWFNAEKGFGFIEVEGGEDVFVHFSAIQGEGFKTLEEGQEVTFEVEQGNRGPQA
CCCCCEEEEECCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCC
TNVVKK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8294017; 10736231 [H]