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The map label for this gene is topA [H]

Identifier: 152975267

GI number: 152975267

Start: 1579084

End: 1581228

Strand: Direct

Name: topA [H]

Synonym: Bcer98_1472

Alternate gene names: 152975267

Gene position: 1579084-1581228 (Clockwise)

Preceding gene: 152975265

Following gene: 152975268

Centisome position: 38.64

GC content: 35.94

Gene sequence:

>2145_bases
ATGAAATTAATTATTGCCGAGAAACCAGATCAAGGTTTAGCTCTTGTTTCACAATTTAAATACCGCCGGAAAGATGGGTA
TTTAGAAGTAGAAGCAAATGAGTTATTTCCAAATGGAGCTTATTGTACATGGGCAATTGGTCATTTAACGCAGTTATGTA
ATCCAGAACATTATCACGCTGAGTGGAAAAAATGGTCGCTTGATACGTTACCGATGATTCCAGAGCGTTTTCAATTTGAA
GTAACAAAGTCGAAATATAAACAATTTAATGTGGTGAAACAGCTGTTACATAATCCGCAAGTGACCGAAATCATTCATGC
AGGTGATGCAGGACGTGAAGGGGAACTGATTGTGCGAAATATTATTAACCTTTGTAATGTGCAAAAGCCGATGAAACGTC
TTTGGATCTCATCTTTGACAAAACAGGCTATTTATCAAGGGTTTAAAAACTTACTTGATGAATCAGATACGATCAATACG
TACTATGAAGCATATACAAGATCTTGCGCCGATTGGGTCGTTGGCATGAATGCATCGCGCGTCTATAGCATTTTACTAAA
GAAAAAAGGAATGAATGATGTTTTTTCTGCTGGACGTGTTCAAACGCCAACATTAGCGTTAATTGTAAAACGTGAAAAAG
AAATAGAAAACTTTAAATCAGAACCGTTTTGGGAAGTGTTCGCAACCTTTAATATTGAAGGTAAGAAATATGAGGGTAAA
TGGGAAAAAGATGGTGAATCCCGCTTAAAAGACCCTGATATGGCGAATAAAATTGCCGCTTTTTGCCAAGGAAAACCAGC
TGAAGTAAAAGAAGTGAAAACGGAGCGCAAGGAATTTCAGCCGCCGCTTTTATTTAATTTGTCATCGCTACAAGCAACGG
CGAATAAAGCATTTAAATTTTCACCGAAAAAAACGTTAGATATTACACAAGCGTTGTATCAAAAAGGGATTGTTTCTTAT
CCTCGTTCTGACTCGAACTATGTAACACAAGGTGAGGCAGCAACGTTTCCTGATATTTTGCAAAAGTTAAGTCAATTTGA
TGAATATAAAGGGGTGTTGCCAGCCCCAATTGAATCGATTAGGAATAATAAGCGTTATGTGAATGAAAAGAAAGTGACAG
ATCACTATGCGATTATACCGACAGAACAAGTTACGAATCCAAATAAGCTATCTGGTGATGAAAAGAAAATTTACGATATG
ATTGTAAGAAGACTGATTGCCGCTCATTATGAAGTTGCTATTTTCGACTATACAACAATAACAACCCTTGTAGATGGACG
GGCTGAATTTATTTCAAAAGGGAAACAACAAATTCAAGAAGGATGGCGGAAAGTCATCTTTCAAGATGATAAGGACGATG
AAACGATCCTTCCAATTGTTACAGAAGGGGAAGAAGGAAAAGTTGTAAAAGTGAAAGTAAAAGAAGGAAAAACGCAGCCG
CCGAAGCGTTATACAGAAGGGCAATTGATTACGTTAATGAAAACGGCAGGAAAATATTTAGATAATGAAGAGCTTGAAAA
AGTGTTAAAGAAAACAGAAGGGTTAGGCACGGAAGCGACTCGGGCTGGTATTATTACAATGCTGAAAGACCGTAAATATA
TAGATGTGAAGAAAAACCAAGTGTATGCAACGGATAAAGGGAAAGTACTTATTACAGCGATTGGGGATAAAATTTTAGCT
TCACCAGAAATGACAGCAAAGTGGGAGCAACGTCTTGCAGAAATTGGAGAAGGAAAAGCATCACCAGCGACTTTCATGGA
ACAAACGAAGAAGCTATCTGCAAAAATTATTGAAGATGCAATTGAAATGTCTGAGAAATGGGATTTCACAGGCTTAGATG
TAGAATCGATTGAACGAAAAGGATCAAAATTTACAACGGGGAAAAAAGTAGGGAAATGTAAAAAATGTGATGGCGATGTG
ATCGATAAGTCTACTTTTTACGGTTGTTCGAACTATAATACAACAAAATGTGATTTTACAATTTCAAAAAAAATATTAGG
CAAAACAATCTCACAAAAAAATATGACAAAACTATTGAAAGGTGAACAAACTGACTTAATTAAAGGATTTAAGAAAAATG
ATAAAACGTTTGATGCAAAGTTAGAATGGAAAGATAATAAAATAAACTTTGTGTTTGAAAATTAA

Upstream 100 bases:

>100_bases
CATAAATACATTGCGAATGATAAAAAGAAAGATTTATGTATCATCGCTTTTAACAGATCAAATGAAAAGTAACACTGGAA
TTTAGATAGGGAGTTATAAA

Downstream 100 bases:

>100_bases
TAGTTGTTAACTAATAAAAAAGTGTTTTACGAATATGAAATGACAAAAATAATACGTCTATGCATTTTTGAAAATAAACC
CCTGATTCCCTTTGAATGAG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVYSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDGESRLKDPDMANKIAAFCQGKPAEVKEVKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGVLPAPIESIRNNKRYVNEKKVTDHYAIIPTEQVTNPNKLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTITTLVDGRAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVTEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLDNEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGKASPATFMEQTKKLSAKIIEDAIEMSEKWDFTGLDVESIERKGSKFTTGKKVGKCKKCDGDV
IDKSTFYGCSNYNTTKCDFTISKKILGKTISQKNMTKLLKGEQTDLIKGFKKNDKTFDAKLEWKDNKINFVFEN

Sequences:

>Translated_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVYSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDGESRLKDPDMANKIAAFCQGKPAEVKEVKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGVLPAPIESIRNNKRYVNEKKVTDHYAIIPTEQVTNPNKLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTITTLVDGRAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVTEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLDNEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGKASPATFMEQTKKLSAKIIEDAIEMSEKWDFTGLDVESIERKGSKFTTGKKVGKCKKCDGDV
IDKSTFYGCSNYNTTKCDFTISKKILGKTISQKNMTKLLKGEQTDLIKGFKKNDKTFDAKLEWKDNKINFVFEN
>Mature_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVYSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDGESRLKDPDMANKIAAFCQGKPAEVKEVKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGVLPAPIESIRNNKRYVNEKKVTDHYAIIPTEQVTNPNKLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTITTLVDGRAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVTEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLDNEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGKASPATFMEQTKKLSAKIIEDAIEMSEKWDFTGLDVESIERKGSKFTTGKKVGKCKKCDGDV
IDKSTFYGCSNYNTTKCDFTISKKILGKTISQKNMTKLLKGEQTDLIKGFKKNDKTFDAKLEWKDNKINFVFEN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=670, Percent_Identity=24.1791044776119, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4507635, Length=458, Percent_Identity=25.5458515283843, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=33.9774557165861, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1787529, Length=633, Percent_Identity=26.8562401263823, Blast_Score=143, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=28.4345047923323, Blast_Score=168, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32563869, Length=556, Percent_Identity=25.7194244604317, Blast_Score=126, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=628, Percent_Identity=25.6369426751592, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=710, Percent_Identity=24.9295774647887, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640096, Length=492, Percent_Identity=24.1869918699187, Blast_Score=104, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81518; Mature: 81518

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VYSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDGESRLKDPDMANKIAAFCQGKPAEVKEVKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGVLPAPIESI
CCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
RNNKRYVNEKKVTDHYAIIPTEQVTNPNKLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
CCCCHHCCCCCCCCCEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEHHHH
TTLVDGRAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVTEGEEGKVVKVKVKEGKTQP
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLDNEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEEHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGKASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
IEMSEKWDFTGLDVESIERKGSKFTTGKKVGKCKKCDGDVIDKSTFYGCSNYNTTKCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCEECCCCCCCCEEEEE
ISKKILGKTISQKNMTKLLKGEQTDLIKGFKKNDKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCEEEEEECC
>Mature Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VYSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDGESRLKDPDMANKIAAFCQGKPAEVKEVKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGVLPAPIESI
CCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
RNNKRYVNEKKVTDHYAIIPTEQVTNPNKLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
CCCCHHCCCCCCCCCEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEHHHH
TTLVDGRAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVTEGEEGKVVKVKVKEGKTQP
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLDNEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEEHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGKASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
IEMSEKWDFTGLDVESIERKGSKFTTGKKVGKCKKCDGDVIDKSTFYGCSNYNTTKCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCEECCCCCCCCEEEEE
ISKKILGKTISQKNMTKLLKGEQTDLIKGFKKNDKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]