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The map label for this gene is ilvE [H]

Identifier: 152975241

GI number: 152975241

Start: 1546884

End: 1547783

Strand: Direct

Name: ilvE [H]

Synonym: Bcer98_1444

Alternate gene names: 152975241

Gene position: 1546884-1547783 (Clockwise)

Preceding gene: 152975239

Following gene: 152975242

Centisome position: 37.85

GC content: 39.0

Gene sequence:

>900_bases
ATGAAAAAGCAGTATATTTATATGAATGGGGAATTAGTAGAGAAGGAAAAGGCTGTTGTTTCGGTATATGATCATGGGTT
TTTATATGGAGACGGTGTATTTGAAGGAATTCGTAGTTACGGTGGAAATGTCTTTTGTTTAAAAGAGCATGTAAAGCGGT
TATATGAATCTGCAAAATCTATTTTGCTCACGATTCCAATGACAGTGGAAGAAATGGAAAAAGCGGTTGTACAGACGTTA
CAAAAAAATGGATATGCCGATGCATATATTCGACTAATTGTTTCAAGAGGAAAGGGGGATTTAGGACTGGATCCAAGAAG
TTGCGAAAAGCCAAGTGTCATCATTATTGCAGAGCAATTAAAATTATTTCCTCAAGAATTTTATGATAATGGTCTGAGCG
TTGTATCGGTTGCATCTCGCCGGAATATGCCAGATGCATTAGATCCACGTATTAAGTCAATGAATTATTTAAATAACGTA
CTAGTGAAAATTGAAGCAGCGCAAGCGGGTGCACTAGAAGCACTTATGTTAAATCAACAAGGATATGTTTGTGAAGGGTC
AGGAGACAATGTTTTTGTAGTGAAAGATGGAAAAGTAGTAACACCGCCTTCTTACTTAGGGGCATTGGAAGGAGTGACTC
GCAATAGCGTGATTGAACTTTGTGACAAATTGGGGATTCCATGTGAAGAAAGACCGTTTACCCGTCACGATGTATATGTA
GCGGATGAAGTGTTTTTAACGGGAACTGCTGCTGAATTAATTCCAGTTGTAAAAGTTGATGCAAGAGAAATTGGAGATGG
AAAGCCTGGAGAGGTAACAAAACAATTAACCGAAGCGTTTAAACGGTTAACGAGAGAACGAGGAGTACGTGTGCCAGGAC
TAGCAGAGAGTTTGGCGTAA

Upstream 100 bases:

>100_bases
TTATGAATAATATACAGAAAATTTAGTCTTTATATATTCTTCTCAGCAATCGATTTAAAGATAGATTGCATAAAGAGATG
AAAAAGAGGAGCGATGTAAG

Downstream 100 bases:

>100_bases
AAGATAGGGGAGGAGTAAAAAATGGAAGATAGGCATACAGTTTGCAAGAAAAAGGAGTTGGAAGAAGGGAAAGAGAGCGG
TGCAAGTCATGTTATTCAAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKKQYIYMNGELVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEKAVVQTL
QKNGYADAYIRLIVSRGKGDLGLDPRSCEKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNMPDALDPRIKSMNYLNNV
LVKIEAAQAGALEALMLNQQGYVCEGSGDNVFVVKDGKVVTPPSYLGALEGVTRNSVIELCDKLGIPCEERPFTRHDVYV
ADEVFLTGTAAELIPVVKVDAREIGDGKPGEVTKQLTEAFKRLTRERGVRVPGLAESLA

Sequences:

>Translated_299_residues
MKKQYIYMNGELVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEKAVVQTL
QKNGYADAYIRLIVSRGKGDLGLDPRSCEKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNMPDALDPRIKSMNYLNNV
LVKIEAAQAGALEALMLNQQGYVCEGSGDNVFVVKDGKVVTPPSYLGALEGVTRNSVIELCDKLGIPCEERPFTRHDVYV
ADEVFLTGTAAELIPVVKVDAREIGDGKPGEVTKQLTEAFKRLTRERGVRVPGLAESLA
>Mature_299_residues
MKKQYIYMNGELVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEKAVVQTL
QKNGYADAYIRLIVSRGKGDLGLDPRSCEKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNMPDALDPRIKSMNYLNNV
LVKIEAAQAGALEALMLNQQGYVCEGSGDNVFVVKDGKVVTPPSYLGALEGVTRNSVIELCDKLGIPCEERPFTRHDVYV
ADEVFLTGTAAELIPVVKVDAREIGDGKPGEVTKQLTEAFKRLTRERGVRVPGLAESLA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=38.6206896551724, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787338, Length=241, Percent_Identity=24.4813278008299, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32998; Mature: 32998

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQYIYMNGELVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKS
CCCCEEEECCEEECCCHHEEEEECCCEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
ILLTIPMTVEEMEKAVVQTLQKNGYADAYIRLIVSRGKGDLGLDPRSCEKPSVIIIAEQL
EEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHH
KLFPQEFYDNGLSVVSVASRRNMPDALDPRIKSMNYLNNVLVKIEAAQAGALEALMLNQQ
HHHHHHHHCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCEEEEEECCC
GYVCEGSGDNVFVVKDGKVVTPPSYLGALEGVTRNSVIELCDKLGIPCEERPFTRHDVYV
CCEEECCCCEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
ADEVFLTGTAAELIPVVKVDAREIGDGKPGEVTKQLTEAFKRLTRERGVRVPGLAESLA
ECEEEEECCHHHHEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure
MKKQYIYMNGELVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKS
CCCCEEEECCEEECCCHHEEEEECCCEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
ILLTIPMTVEEMEKAVVQTLQKNGYADAYIRLIVSRGKGDLGLDPRSCEKPSVIIIAEQL
EEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHH
KLFPQEFYDNGLSVVSVASRRNMPDALDPRIKSMNYLNNVLVKIEAAQAGALEALMLNQQ
HHHHHHHHCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCEEEEEECCC
GYVCEGSGDNVFVVKDGKVVTPPSYLGALEGVTRNSVIELCDKLGIPCEERPFTRHDVYV
CCEEECCCCEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
ADEVFLTGTAAELIPVVKVDAREIGDGKPGEVTKQLTEAFKRLTRERGVRVPGLAESLA
ECEEEEECCHHHHEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]