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The map label for this gene is nth [H]

Identifier: 152975070

GI number: 152975070

Start: 1377938

End: 1378585

Strand: Direct

Name: nth [H]

Synonym: Bcer98_1270

Alternate gene names: 152975070

Gene position: 1377938-1378585 (Clockwise)

Preceding gene: 152975069

Following gene: 152975071

Centisome position: 33.71

GC content: 35.96

Gene sequence:

>648_bases
ATGTTAAATAAAACACAAATTCGCTATTGTCTAGATACAATGGCGAAAATGTATCCAGAGGCACATTGTGAATTAATTCA
TGATAATCCATTTGAACTTGTCATTGCAGTGGCTTTATCTGCACAATGTACAGATGCACTTGTAAATAAAGTGACGAAAA
ATTTATTTCAAAAATATAAAACACCTGAGGACTATTTGAAAGTTTCTTTGGAAGAATTACAACAAGACATACGTTCCATA
GGGCTTTATAGAAATAAGGCAAAAAATATTCAAAAGCTATGCAGAATGCTAATCGATGAGTATGATGGGAAAGTTCCTGC
AGATCGTGATGAATTAACAAAATTACCAGGGGTAGGTCGAAAGACGGCAAATGTAGTTGTTTCTGTTGCTTTTGGCATTC
CAGCAATTGCAGTCGATACACATGTGGAACGAGTGAGTAAGCGTTTAGGAATTTGTCGATGGAAAGACTCTGTGTTAGAA
GTTGAAAAAACATTAATGAAAAAGGTGCCTATGGAAGAATGGGGCGTTACGCATCATAGACTGATTTTTTTTGGACGTTA
TCATTGTAAAGCACAAAAGCCACAGTGTGAAGTATGTCCGTTATTAGAAATATGTCGTGAAGGTAAAAAGCGGATGAAGG
TGAAGTAG

Upstream 100 bases:

>100_bases
AAATCAGCAACGCTCACATCAGATAACAAAACAAGAGACAAAGTACACAGGGAAAGTCCCTTTTTATAATTGGTTGGAGC
AATAAGGTAGGAGGAAATGT

Downstream 100 bases:

>100_bases
GGAATGGAAAACAGAGTTGTGGAGATACCACCTGAGTTTCAATGTGAGCCGTTTTTCAAAGAAAGTGAAACAAAAATTGT
GTACACATGTAGTCAATCAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MLNKTQIRYCLDTMAKMYPEAHCELIHDNPFELVIAVALSAQCTDALVNKVTKNLFQKYKTPEDYLKVSLEELQQDIRSI
GLYRNKAKNIQKLCRMLIDEYDGKVPADRDELTKLPGVGRKTANVVVSVAFGIPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMKKVPMEEWGVTHHRLIFFGRYHCKAQKPQCEVCPLLEICREGKKRMKVK

Sequences:

>Translated_215_residues
MLNKTQIRYCLDTMAKMYPEAHCELIHDNPFELVIAVALSAQCTDALVNKVTKNLFQKYKTPEDYLKVSLEELQQDIRSI
GLYRNKAKNIQKLCRMLIDEYDGKVPADRDELTKLPGVGRKTANVVVSVAFGIPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMKKVPMEEWGVTHHRLIFFGRYHCKAQKPQCEVCPLLEICREGKKRMKVK
>Mature_215_residues
MLNKTQIRYCLDTMAKMYPEAHCELIHDNPFELVIAVALSAQCTDALVNKVTKNLFQKYKTPEDYLKVSLEELQQDIRSI
GLYRNKAKNIQKLCRMLIDEYDGKVPADRDELTKLPGVGRKTANVVVSVAFGIPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMKKVPMEEWGVTHHRLIFFGRYHCKAQKPQCEVCPLLEICREGKKRMKVK

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=174, Percent_Identity=32.183908045977, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI190358497, Length=189, Percent_Identity=27.5132275132275, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI6912520, Length=189, Percent_Identity=27.5132275132275, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI115298648, Length=189, Percent_Identity=27.5132275132275, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI115298650, Length=189, Percent_Identity=28.042328042328, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI115298654, Length=189, Percent_Identity=28.042328042328, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI115298652, Length=189, Percent_Identity=28.042328042328, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=45.933014354067, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1789331, Length=187, Percent_Identity=24.0641711229947, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=35.7541899441341, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=197, Percent_Identity=25.8883248730964, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=180, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550361, Length=190, Percent_Identity=32.1052631578947, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24763; Mature: 24763

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKTQIRYCLDTMAKMYPEAHCELIHDNPFELVIAVALSAQCTDALVNKVTKNLFQKYK
CCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPEDYLKVSLEELQQDIRSIGLYRNKAKNIQKLCRMLIDEYDGKVPADRDELTKLPGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
KTANVVVSVAFGIPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMKKVPMEEWGVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHH
LIFFGRYHCKAQKPQCEVCPLLEICREGKKRMKVK
HHEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNKTQIRYCLDTMAKMYPEAHCELIHDNPFELVIAVALSAQCTDALVNKVTKNLFQKYK
CCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPEDYLKVSLEELQQDIRSIGLYRNKAKNIQKLCRMLIDEYDGKVPADRDELTKLPGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
KTANVVVSVAFGIPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMKKVPMEEWGVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHH
LIFFGRYHCKAQKPQCEVCPLLEICREGKKRMKVK
HHEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]