Click here to switch to the map view.

The map label for this gene is gudB [H]

Identifier: 152975014

GI number: 152975014

Start: 1327665

End: 1328948

Strand: Direct

Name: gudB [H]

Synonym: Bcer98_1214

Alternate gene names: 152975014

Gene position: 1327665-1328948 (Clockwise)

Preceding gene: 152975013

Following gene: 152975015

Centisome position: 32.48

GC content: 38.08

Gene sequence:

>1284_bases
ATGGTAGCCGAAAAGGGAACTCAAACACAAACACAACAGAGGGAACAACATTTTGAATTGTTGAATTCAACACAAATTGT
TATTAATGAAGCATTAGAAAAATTGGGTTATCCAAACGAAGTATATGAATTACTAAAAGAGCCGGTTCGTATGATGACAG
TAAAAATTCCAGTTCGTATGGACGATGGGACTGTTAAAATATTTACAGGGTATCGTGCGCAGCACAATGACGCTGTTGGT
CCAACGAAAGGTGGAATCCGCTTCCATCCAAATGTAACAGAAAATGAAGTGAAAGCACTTTCAATCTGGATGAGCTTAAA
ATGTGGTATCGTTGATTTACCATATGGTGGAGGAAAAGGCGGAATCGTTTGTGATCCGCGTGAGATGTCTTTCCGTGAAT
TAGAAAGATTAAGTCGTGGATATGTACGAGCAATTAGCCAAATTGTTGGCCCAACAAAAGACATTCCTGCTCCAGACGTA
TTTACAAACTCACAAATTATGGCATGGATGATGGATGAATATAGCCGTATTGATGAATTTAATTCACCAGGATTCATTAC
TGGTAAGCCGCTTGTGTTAGGTGGTTCCCATGGACGTGAAACAGCGACTGCAAAAGGTGTAACGATTTGTATTCGTGAAG
CTGCGAAAAAACGCGGTATTGATATTAAGGGAGCTCGTGTTGTTGTTCAAGGATTTGGTAACGCTGGTAGTTTCTTAGCT
AAGTTTATGCATGATGCGGGTGCAAAAGTAATTGCCATTTCAGATGCTTATGGTGCACTGCACGATCCAAATGGATTGGA
CATTGATTATTTATTAGATCGTCGCGATAGCTTTGGTACCGTAACAAAATTGTTTAACAATACAATTACAAATAAAGAAT
TGTTAGAACTGGAATGTGATATTTTAGTTCCAGCTGCAATTGAAAATCAAATAACAGAAAAAAATGCCGCTGATATTAAA
GCGAAAATTGTAGTAGAAGCAGCAAATGGCCCAACTACATTAGAAGCAACAAAAATTTTAACAGATCGCGGTATTCTTCT
TGTTCCTGACGTACTAGCAAGTGCTGGTGGAGTTACAGTATCTTACTTTGAGTGGGTACAAAATAATCAAGGTTATTATT
GGTCAGAAGAAGAAGTTGAAGAGCGTTTAGAAAAAGTAATGGTAAAATCATTTGAGTCTATTTATGAAACATCTCAAGTT
CGTAAAGTGAATATGCGTTTAGCAGCTTATATGGTTGGTGTGCGTAAAATGGCAGAAGCTAGCCGCTTTAGAGGTTGGGT
ATAA

Upstream 100 bases:

>100_bases
ATATATTTGTTAGCGTTTTCTATTGCATTCAAAATTTAACGGTGTATACTAGGCAATGAAGGTTTACTAAACAAGTGAAA
TTACAGGGGGTTTACTTACT

Downstream 100 bases:

>100_bases
TAGAATAGAGTATTTGGAAAAGCGTGGTTTCCTCAAAAGGAAACCACGCTTTTTTTAGGTGCAAAATGAAGTGAATATAG
CTAGTTGAGAAGTTAGAAAG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MVAEKGTQTQTQQREQHFELLNSTQIVINEALEKLGYPNEVYELLKEPVRMMTVKIPVRMDDGTVKIFTGYRAQHNDAVG
PTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKGGIVCDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDV
FTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFLA
KFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTITNKELLELECDILVPAAIENQITEKNAADIK
AKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVEERLEKVMVKSFESIYETSQV
RKVNMRLAAYMVGVRKMAEASRFRGWV

Sequences:

>Translated_427_residues
MVAEKGTQTQTQQREQHFELLNSTQIVINEALEKLGYPNEVYELLKEPVRMMTVKIPVRMDDGTVKIFTGYRAQHNDAVG
PTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKGGIVCDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDV
FTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFLA
KFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTITNKELLELECDILVPAAIENQITEKNAADIK
AKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVEERLEKVMVKSFESIYETSQV
RKVNMRLAAYMVGVRKMAEASRFRGWV
>Mature_427_residues
MVAEKGTQTQTQQREQHFELLNSTQIVINEALEKLGYPNEVYELLKEPVRMMTVKIPVRMDDGTVKIFTGYRAQHNDAVG
PTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKGGIVCDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDV
FTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFLA
KFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTITNKELLELECDILVPAAIENQITEKNAADIK
AKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVEERLEKVMVKSFESIYETSQV
RKVNMRLAAYMVGVRKMAEASRFRGWV

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=40.1759530791789, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=39.8826979472141, Blast_Score=265, Evalue=9e-71,
Organism=Escherichia coli, GI1788059, Length=406, Percent_Identity=30.7881773399015, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=340, Percent_Identity=40.8823529411765, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319256, Length=398, Percent_Identity=28.1407035175879, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324951, Length=382, Percent_Identity=27.4869109947644, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45553475, Length=340, Percent_Identity=42.3529411764706, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=42.3529411764706, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24649283, Length=371, Percent_Identity=40.1617250673854, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=41.5770609318996, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 47337; Mature: 47337

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAEKGTQTQTQQREQHFELLNSTQIVINEALEKLGYPNEVYELLKEPVRMMTVKIPVRM
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEEEE
DDGTVKIFTGYRAQHNDAVGPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKG
CCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHEEEEEEEECCEEECCCCCCCC
GIVCDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSRIDEF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFLA
CCCCEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHH
KFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTITNKELLELECD
HHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCHHEEEEEC
ILVPAAIENQITEKNAADIKAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTV
EEECHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEHHHHHHCCCEEEECHHHHCCCCCEE
SYFEWVQNNQGYYWSEEEVEERLEKVMVKSFESIYETSQVRKVNMRLAAYMVGVRKMAEA
EEHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRFRGWV
HHHCCCC
>Mature Secondary Structure
MVAEKGTQTQTQQREQHFELLNSTQIVINEALEKLGYPNEVYELLKEPVRMMTVKIPVRM
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEEEE
DDGTVKIFTGYRAQHNDAVGPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKG
CCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHEEEEEEEECCEEECCCCCCCC
GIVCDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSRIDEF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFLA
CCCCEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHH
KFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTITNKELLELECD
HHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCHHEEEEEC
ILVPAAIENQITEKNAADIKAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTV
EEECHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEHHHHHHCCCEEEECHHHHCCCCCEE
SYFEWVQNNQGYYWSEEEVEERLEKVMVKSFESIYETSQVRKVNMRLAAYMVGVRKMAEA
EEHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRFRGWV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]