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The map label for this gene is pbpF [H]

Identifier: 152974980

GI number: 152974980

Start: 1292384

End: 1294423

Strand: Direct

Name: pbpF [H]

Synonym: Bcer98_1180

Alternate gene names: 152974980

Gene position: 1292384-1294423 (Clockwise)

Preceding gene: 152974976

Following gene: 152974983

Centisome position: 31.62

GC content: 36.13

Gene sequence:

>2040_bases
ATGAAATTGGACAACACTCATTTTAAGAACGTGCTTGAGTGGTTCAACAAGAGAAAGAAACTGCGCAATACATTATTGAT
TTTAGGTGGTTTATTCATAACTCTCTTTGTTGCAATAAATATAATGATATCCATTCAAGACATATCAGCATTAAAACAAG
CAGTTCCACAACCAACACTTATTTATGATGCAAATAATGAAGTGGCAGCTAAGTTATCGTCTTCTAAAACAGAAGGAATT
AAAAGAAAAGACATACCTGATATTATGGTTCAAGCCATAATATCAGTAGAAGACAAACATTTTTATAACCATCATGGAAT
TTATTATAGTGGAATTTTGAATGCTATATTAAAGAATGTTACTGCGGGTGAAGTCATTGCGGGAGGGAGTACAATCACGC
AACAGCTTGCGAAAAATGTATTTTTAACACAAGAACGAACATTTTCAAGGAAATTTAAAGAATATTTTTTAACGAAAAAA
ATAGAGCGCACATATACGAAAGATGAAATTATTGAGATGTATATGAATCAAATTTATTTTGGTGAAGGCGCTTGGGGGAT
AAGGAAAGCTGCTAAATCATATTTTGATAAAGAAGTGAAAGACTTAACGATAGCAGAGGCTGCTACCATAGCTGGTCTTA
TTAAGGCTCCGTCTACTTACTCCCCCTATAAAAATTTCAATAAATCCATTGAAAGACGGAATATTGTGCTAGCTTTAATG
AAGGAGCAAGGGTATATTTCGGAGGAACAATATAGCAAAGAAAAAGAAAAAGGACTTGTGCTACGCCGAGGTGTAGATGA
TAAATATAAAGGGAAATACTCACAATATGTAGATTATATTGTAAGAGAAGCAATGGAGAAATATGATTTAACCCAAAATG
AAATTTTAGCTGGTGGTTATCACATTTATACCGAACTTGATCCTAAAAAACAGCAAGCGGTAGAAGATGTTGTTAATAAT
AATAGGTATTTTAAAGATCGTAGCTCAGATCAATTACTTCAAACAGGCATTGTACTGATGAACCCGAAAACAGGTGGTGT
TCCGGCACTAGTTGGTAGCAGGGGACCATATCAATTTTTACAATTTAATCATGCGACGCAATTAAAGCGTCAACCAGGAT
CAACGTTAAAGCCGTTTGCAGTGTACGTACCAGCCTTAGAAGAAGGGTATGAAGTATATGATATATTGAAGGATGAACCA
CTGCATATAAAAGAATATGCACCTCAAAACAGCGATCATATTTTTCATGGGGATGTGACGATGTACGAAGCGATTGCAAA
GTCGTATAACGTATCAGCTGTTTGGCTATTGAAACAAATCGGTTTAGAAAAAGGGCTGAAATCGTTAGAGCGTTTTGGTA
TTCCGTTGCAAGAAGAAGATCGTACGTATCCTATCGCACTCGGTGGAATGCATACAGGGACTTCCCCATTTGTTATGGCT
CAAGCGTATGCGACATTTGCTAATAATGGAGTGCAGGTAGAGGGACATGCAATTCGCGAAATAAAAAATACTGAAGGCGA
AACAGTAGGGAAATGGTATAAAAAAGAAACACGTATAACAACCGAAAAAGTTGCCCAAAAGATGACATATTTATTAAAGG
GTGTTGTTGAAAAAGGAACGGGTGAAAAAGCAAAGCTGAAAAATATTGATACAGCAGGAAAAACAGGAACAACTCAAATT
GTAGGTGGTCCAAGCAGCGGGGCAAAAGATTCATGGTTTGTAGGGTATACCCCAGACTTAGTAGGAGCAATTTGGGTTGG
ATATGATAAGACAGACCATGAGCATTATGTGCCAAGTGGCAGTCATATTACAACAACGATGTTTCGGGATATTATGAACA
AAGTAGGAGAAAAAGCATCTCAAAAAGCATTCCAGTTATCCTTAATACCGGAGGCTGAATATACAAAACAGTTACAAACA
ATTGAAGAAGAAAAAAGAAGGAAAGAAGAGGAGAAAAGAAGAAAAGAGGAAGAACAGCAAAGAAAGGAACAACAGAAAGA
ATGGATTGATAAGGTAAAAGAGTGGATTCCATTTTTTTGA

Upstream 100 bases:

>100_bases
CTTTACACCTAAGAATGGATTACGATATTATAATGAATTTAAAAGGAGGGAGTTTCTTCCTTTTAATACAAGAGTGTAAA
GGAATGAAGGAAGTGGATAA

Downstream 100 bases:

>100_bases
AAAGAGGCGATGGAAACGTTCGCCTCTCTTTTTATGGCATGTGGATGTTTATATTTGCTGCAGTTGATGATGTATAAATA
ATCGAAATGGTTCCTTTACG

Product: 1A family penicillin-binding protein

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MKLDNTHFKNVLEWFNKRKKLRNTLLILGGLFITLFVAINIMISIQDISALKQAVPQPTLIYDANNEVAAKLSSSKTEGI
KRKDIPDIMVQAIISVEDKHFYNHHGIYYSGILNAILKNVTAGEVIAGGSTITQQLAKNVFLTQERTFSRKFKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIRKAAKSYFDKEVKDLTIAEAATIAGLIKAPSTYSPYKNFNKSIERRNIVLALM
KEQGYISEEQYSKEKEKGLVLRRGVDDKYKGKYSQYVDYIVREAMEKYDLTQNEILAGGYHIYTELDPKKQQAVEDVVNN
NRYFKDRSSDQLLQTGIVLMNPKTGGVPALVGSRGPYQFLQFNHATQLKRQPGSTLKPFAVYVPALEEGYEVYDILKDEP
LHIKEYAPQNSDHIFHGDVTMYEAIAKSYNVSAVWLLKQIGLEKGLKSLERFGIPLQEEDRTYPIALGGMHTGTSPFVMA
QAYATFANNGVQVEGHAIREIKNTEGETVGKWYKKETRITTEKVAQKMTYLLKGVVEKGTGEKAKLKNIDTAGKTGTTQI
VGGPSSGAKDSWFVGYTPDLVGAIWVGYDKTDHEHYVPSGSHITTTMFRDIMNKVGEKASQKAFQLSLIPEAEYTKQLQT
IEEEKRRKEEEKRRKEEEQQRKEQQKEWIDKVKEWIPFF

Sequences:

>Translated_679_residues
MKLDNTHFKNVLEWFNKRKKLRNTLLILGGLFITLFVAINIMISIQDISALKQAVPQPTLIYDANNEVAAKLSSSKTEGI
KRKDIPDIMVQAIISVEDKHFYNHHGIYYSGILNAILKNVTAGEVIAGGSTITQQLAKNVFLTQERTFSRKFKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIRKAAKSYFDKEVKDLTIAEAATIAGLIKAPSTYSPYKNFNKSIERRNIVLALM
KEQGYISEEQYSKEKEKGLVLRRGVDDKYKGKYSQYVDYIVREAMEKYDLTQNEILAGGYHIYTELDPKKQQAVEDVVNN
NRYFKDRSSDQLLQTGIVLMNPKTGGVPALVGSRGPYQFLQFNHATQLKRQPGSTLKPFAVYVPALEEGYEVYDILKDEP
LHIKEYAPQNSDHIFHGDVTMYEAIAKSYNVSAVWLLKQIGLEKGLKSLERFGIPLQEEDRTYPIALGGMHTGTSPFVMA
QAYATFANNGVQVEGHAIREIKNTEGETVGKWYKKETRITTEKVAQKMTYLLKGVVEKGTGEKAKLKNIDTAGKTGTTQI
VGGPSSGAKDSWFVGYTPDLVGAIWVGYDKTDHEHYVPSGSHITTTMFRDIMNKVGEKASQKAFQLSLIPEAEYTKQLQT
IEEEKRRKEEEKRRKEEEQQRKEQQKEWIDKVKEWIPFF
>Mature_679_residues
MKLDNTHFKNVLEWFNKRKKLRNTLLILGGLFITLFVAINIMISIQDISALKQAVPQPTLIYDANNEVAAKLSSSKTEGI
KRKDIPDIMVQAIISVEDKHFYNHHGIYYSGILNAILKNVTAGEVIAGGSTITQQLAKNVFLTQERTFSRKFKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIRKAAKSYFDKEVKDLTIAEAATIAGLIKAPSTYSPYKNFNKSIERRNIVLALM
KEQGYISEEQYSKEKEKGLVLRRGVDDKYKGKYSQYVDYIVREAMEKYDLTQNEILAGGYHIYTELDPKKQQAVEDVVNN
NRYFKDRSSDQLLQTGIVLMNPKTGGVPALVGSRGPYQFLQFNHATQLKRQPGSTLKPFAVYVPALEEGYEVYDILKDEP
LHIKEYAPQNSDHIFHGDVTMYEAIAKSYNVSAVWLLKQIGLEKGLKSLERFGIPLQEEDRTYPIALGGMHTGTSPFVMA
QAYATFANNGVQVEGHAIREIKNTEGETVGKWYKKETRITTEKVAQKMTYLLKGVVEKGTGEKAKLKNIDTAGKTGTTQI
VGGPSSGAKDSWFVGYTPDLVGAIWVGYDKTDHEHYVPSGSHITTTMFRDIMNKVGEKASQKAFQLSLIPEAEYTKQLQT
IEEEKRRKEEEKRRKEEEQQRKEQQKEWIDKVKEWIPFF

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=530, Percent_Identity=31.8867924528302, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082258, Length=282, Percent_Identity=39.3617021276596, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1788867, Length=531, Percent_Identity=28.4369114877589, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=33.5227272727273, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 77446; Mature: 77446

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDNTHFKNVLEWFNKRKKLRNTLLILGGLFITLFVAINIMISIQDISALKQAVPQPTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYDANNEVAAKLSSSKTEGIKRKDIPDIMVQAIISVEDKHFYNHHGIYYSGILNAILKNV
EEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
TAGEVIAGGSTITQQLAKNVFLTQERTFSRKFKEYFLTKKIERTYTKDEIIEMYMNQIYF
CCCCEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEE
GEGAWGIRKAAKSYFDKEVKDLTIAEAATIAGLIKAPSTYSPYKNFNKSIERRNIVLALM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEE
KEQGYISEEQYSKEKEKGLVLRRGVDDKYKGKYSQYVDYIVREAMEKYDLTQNEILAGGY
CCCCCCCHHHHHHHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHEEECCE
HIYTELDPKKQQAVEDVVNNNRYFKDRSSDQLLQTGIVLMNPKTGGVPALVGSRGPYQFL
EEEECCCCHHHHHHHHHHCCCCEECCCCHHHHHHCCEEEECCCCCCCCEECCCCCCHHHH
QFNHATQLKRQPGSTLKPFAVYVPALEEGYEVYDILKDEPLHIKEYAPQNSDHIFHGDVT
HCCCHHHHHCCCCCCCCCCEEEECCHHCCCHHHHHHCCCCCCHHHCCCCCCCEEEECCHH
MYEAIAKSYNVSAVWLLKQIGLEKGLKSLERFGIPLQEEDRTYPIALGGMHTGTSPFVMA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHH
QAYATFANNGVQVEGHAIREIKNTEGETVGKWYKKETRITTEKVAQKMTYLLKGVVEKGT
HHHHHHCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GEKAKLKNIDTAGKTGTTQIVGGPSSGAKDSWFVGYTPDLVGAIWVGYDKTDHEHYVPSG
CCCHHHCCCCCCCCCCCEEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCC
SHITTTMFRDIMNKVGEKASQKAFQLSLIPEAEYTKQLQTIEEEKRRKEEEKRRKEEEQQ
CCHHHHHHHHHHHHHCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKEQQKEWIDKVKEWIPFF
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLDNTHFKNVLEWFNKRKKLRNTLLILGGLFITLFVAINIMISIQDISALKQAVPQPTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYDANNEVAAKLSSSKTEGIKRKDIPDIMVQAIISVEDKHFYNHHGIYYSGILNAILKNV
EEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
TAGEVIAGGSTITQQLAKNVFLTQERTFSRKFKEYFLTKKIERTYTKDEIIEMYMNQIYF
CCCCEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEE
GEGAWGIRKAAKSYFDKEVKDLTIAEAATIAGLIKAPSTYSPYKNFNKSIERRNIVLALM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEE
KEQGYISEEQYSKEKEKGLVLRRGVDDKYKGKYSQYVDYIVREAMEKYDLTQNEILAGGY
CCCCCCCHHHHHHHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHEEECCE
HIYTELDPKKQQAVEDVVNNNRYFKDRSSDQLLQTGIVLMNPKTGGVPALVGSRGPYQFL
EEEECCCCHHHHHHHHHHCCCCEECCCCHHHHHHCCEEEECCCCCCCCEECCCCCCHHHH
QFNHATQLKRQPGSTLKPFAVYVPALEEGYEVYDILKDEPLHIKEYAPQNSDHIFHGDVT
HCCCHHHHHCCCCCCCCCCEEEECCHHCCCHHHHHHCCCCCCHHHCCCCCCCEEEECCHH
MYEAIAKSYNVSAVWLLKQIGLEKGLKSLERFGIPLQEEDRTYPIALGGMHTGTSPFVMA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHH
QAYATFANNGVQVEGHAIREIKNTEGETVGKWYKKETRITTEKVAQKMTYLLKGVVEKGT
HHHHHHCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GEKAKLKNIDTAGKTGTTQIVGGPSSGAKDSWFVGYTPDLVGAIWVGYDKTDHEHYVPSG
CCCHHHCCCCCCCCCCCEEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCC
SHITTTMFRDIMNKVGEKASQKAFQLSLIPEAEYTKQLQTIEEEKRRKEEEKRRKEEEQQ
CCHHHHHHHHHHHHHCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKEQQKEWIDKVKEWIPFF
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]