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The map label for this gene is liaS [H]

Identifier: 152974962

GI number: 152974962

Start: 1270579

End: 1271646

Strand: Direct

Name: liaS [H]

Synonym: Bcer98_1159

Alternate gene names: 152974962

Gene position: 1270579-1271646 (Clockwise)

Preceding gene: 152974961

Following gene: 152974963

Centisome position: 31.09

GC content: 36.24

Gene sequence:

>1068_bases
ATGATGAAAAAACAAGGAAATATTTCATGGATGTACATTCGTTATTCTATGCTATCTTCCATTAGTATTGCACTCATTTG
TACTATTATCTATATGTGGAAAAGCAAGCAAGATATATATGATTTCCTATGGAAAGAATCCATCGCCTCTGTTCCTGTTG
GTTTGTTAATCATGAGTACAAGTCTTCTTATTGGAGGCATTGTAGGATATGCGATTGGCTATTATGTAAAGCAGCGTATT
CAAGGTTTGAATACTTTCTTATTTGAAGTAGAGCGAGGGAACTTTCCGAAAGATGTTTCGTTTACGGCAGAAGACGAATT
TCATGAAATAGAGCGAAAAGTTATGACACTTACTCGAAGATTAGAAGAACAAGCTGGATTATTTCAAAAGGTAACAAATG
AACGAGCCCATTGGAATGAAGAAATGAAGCAAGAAGCTATTTCTCAAGAAAGACATCGCTTGGCAAGAGAACTGCATGAT
TCTGTAAGTCAGCAGCTTTTTGCAATGTCTATGATGATGTCAGCAATTAATGAGCAAGTAGATCAATTTCCAGAAACAAC
AAAAAAACAATTGAAGCTTGTAGAGAACATGGTCGTGAATGCGCAATCCGAAATGAGAGCATTGCTTCTTCATTTACGTC
CTGTACAATTGGAAGGAAAAAAACTCACAGAAGGTATAGAGGAACTATTAACAGAGCTGTCTAGAAAACAGCATATGAAA
ATCGAATGGTTCGTTGAATCCATTCAGTTAAAAAAAGGTGTCGAAGATCATCTATTTCGTATTTTACAAGAAGCATTGTC
TAACACACTGCGACATGCAAAGGCAAAGAAAATAGAAGTACGCCTTCGTCAAATTGATCAATATGCCATTTTGAAAATAA
TTGATGATGGTGTTGGGTTTGAAGTTGGAGTTGGAGTTGGAGTAAATAAAGCAGGATCATATGGATTACAATCTATGCAG
GAACGTGTTCATGAAATTGGAGGGACGTTAAAAGTACTTAGTTTCCCAAATAAAGGAGCACAAATTGAAGTGAAAGTGCC
AATTATGATAGAGAGAGGGGGAGAATGA

Upstream 100 bases:

>100_bases
ACCGAAATGTTACATTTAAAACAGAGCAGTATAGAGAAGCTCCTCGTCGTATTAAAATTATTTCTTCGCTTGTTGTAGGA
GATACGGAAGTGAGGAAAGT

Downstream 100 bases:

>100_bases
TGATTAAAGTATTACTAGTTGATGATCATGAAATGGTTCGCATGGGAGTATCTGCTTACTTATCAACACAACCAGATATT
GAAGTAGTTGGGGAAGCGGA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MMKKQGNISWMYIRYSMLSSISIALICTIIYMWKSKQDIYDFLWKESIASVPVGLLIMSTSLLIGGIVGYAIGYYVKQRI
QGLNTFLFEVERGNFPKDVSFTAEDEFHEIERKVMTLTRRLEEQAGLFQKVTNERAHWNEEMKQEAISQERHRLARELHD
SVSQQLFAMSMMMSAINEQVDQFPETTKKQLKLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK
IEWFVESIQLKKGVEDHLFRILQEALSNTLRHAKAKKIEVRLRQIDQYAILKIIDDGVGFEVGVGVGVNKAGSYGLQSMQ
ERVHEIGGTLKVLSFPNKGAQIEVKVPIMIERGGE

Sequences:

>Translated_355_residues
MMKKQGNISWMYIRYSMLSSISIALICTIIYMWKSKQDIYDFLWKESIASVPVGLLIMSTSLLIGGIVGYAIGYYVKQRI
QGLNTFLFEVERGNFPKDVSFTAEDEFHEIERKVMTLTRRLEEQAGLFQKVTNERAHWNEEMKQEAISQERHRLARELHD
SVSQQLFAMSMMMSAINEQVDQFPETTKKQLKLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK
IEWFVESIQLKKGVEDHLFRILQEALSNTLRHAKAKKIEVRLRQIDQYAILKIIDDGVGFEVGVGVGVNKAGSYGLQSMQ
ERVHEIGGTLKVLSFPNKGAQIEVKVPIMIERGGE
>Mature_355_residues
MMKKQGNISWMYIRYSMLSSISIALICTIIYMWKSKQDIYDFLWKESIASVPVGLLIMSTSLLIGGIVGYAIGYYVKQRI
QGLNTFLFEVERGNFPKDVSFTAEDEFHEIERKVMTLTRRLEEQAGLFQKVTNERAHWNEEMKQEAISQERHRLARELHD
SVSQQLFAMSMMMSAINEQVDQFPETTKKQLKLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK
IEWFVESIQLKKGVEDHLFRILQEALSNTLRHAKAKKIEVRLRQIDQYAILKIIDDGVGFEVGVGVGVNKAGSYGLQSMQ
ERVHEIGGTLKVLSFPNKGAQIEVKVPIMIERGGE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788812, Length=206, Percent_Identity=27.1844660194175, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082311, Length=215, Percent_Identity=29.7674418604651, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40794; Mature: 40794

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKQGNISWMYIRYSMLSSISIALICTIIYMWKSKQDIYDFLWKESIASVPVGLLIMST
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
SLLIGGIVGYAIGYYVKQRIQGLNTFLFEVERGNFPKDVSFTAEDEFHEIERKVMTLTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LEEQAGLFQKVTNERAHWNEEMKQEAISQERHRLARELHDSVSQQLFAMSMMMSAINEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQFPETTKKQLKLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK
HHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCH
IEWFVESIQLKKGVEDHLFRILQEALSNTLRHAKAKKIEVRLRQIDQYAILKIIDDGVGF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVGVGVGVNKAGSYGLQSMQERVHEIGGTLKVLSFPNKGAQIEVKVPIMIERGGE
EEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEECCCC
>Mature Secondary Structure
MMKKQGNISWMYIRYSMLSSISIALICTIIYMWKSKQDIYDFLWKESIASVPVGLLIMST
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
SLLIGGIVGYAIGYYVKQRIQGLNTFLFEVERGNFPKDVSFTAEDEFHEIERKVMTLTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LEEQAGLFQKVTNERAHWNEEMKQEAISQERHRLARELHDSVSQQLFAMSMMMSAINEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQFPETTKKQLKLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK
HHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCH
IEWFVESIQLKKGVEDHLFRILQEALSNTLRHAKAKKIEVRLRQIDQYAILKIIDDGVGF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVGVGVGVNKAGSYGLQSMQERVHEIGGTLKVLSFPNKGAQIEVKVPIMIERGGE
EEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]