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The map label for this gene is mleN [H]

Identifier: 152974958

GI number: 152974958

Start: 1266747

End: 1268231

Strand: Direct

Name: mleN [H]

Synonym: Bcer98_1155

Alternate gene names: 152974958

Gene position: 1266747-1268231 (Clockwise)

Preceding gene: 152974957

Following gene: 152974959

Centisome position: 30.99

GC content: 37.44

Gene sequence:

>1485_bases
ATGATGCCTCGGCAGCGGACTCGATTTTGGAGTGCTGTGCCAAATCCAGCAAGCATGTGCTTGAAAGATGAGAAGAGTGT
TTCTTATAGATGTATAAGACCTCTTCTTCTTGGAAGAGGTCTTTTGTTATTCAGTAGAAAAGGTTTAAAACTAGGGAGAG
ATGATATGGTGAAGGAAACGAGAGGAAATATTTTTGCTTTATTGCCACTTGGAATATTTTTAGTACTATTTATTGGTTCT
GGAATCATTACAGGTGATTTTTATAAATTGCCGATTTTAGTAGCGATACTAATTGCGGTAGGAGTTGCTTTAGCGATGAA
TCGGAAAGAGAGTTTTACAGCGAAAGTAGAACGCTTCGCTAAAGGAGCTGGGAATCCAGACATTATGATTATGGTATTAA
TATTTGTACTTGCTGGTGCTTTTTCAGAAACAGCAAAGGGAATGGGAGGAGTGGACTCTACAGTTAATTTAGCTTTAGCT
GTTTTGCCACAAGGATTTATTATAGCTGGCATTTTCGTGATCGGTGCATTTATTTCATTAGCAATGGGAACTTCAATGGG
AACAATTGCAGCATTAGCGCCGATTGCTGTAGGGATCAGTCAACAAACAGATATTTCAATTGCGTTAGCGATGGCAACAG
TTGTAGGTGGGGCTATGTTTGGAGATAATTTATCATTTATTTCTGATACAACAATCGCAGCTGTTCGTTCACAAGGGACA
GAAATGAAAGATAAGTTTAAAACGAACTTTTTAATTGTTTTACCCGCTGCTATGATTACCGTTATATTATTAGTTGTTCT
TACTTTAGGAAATCATACAGAAATAAAGACACATGCATTTGATTGGATGAAAATATTGCCGTATGCAGGGGTGCTTGTTA
CAGCGCTACTTGGATGGAATGTACTCGTTGTATTAACAGGTGGAACCATTTTATCTGGTATCATTGGTTTTTTAGATGGG
AGTTATACGTTAGCTAGCTTCTTTAAAAGTGCTGCAACAGGGATGAATGGAATGATGGAACTCGTACTATTGGCAATTTT
AATTGGTGGAATGGTTGAAATCATTCAATATAATGGTGGTATTCAATATTTAATGAATACATTAACGCGTAATATCCGTT
CGAAAAAAGGTGCGGAATTTGGAATTGCTGGATTAGTAAGCATGACGAATATATGCACAGCGAATAACACAATTTCGATT
ATATTCACTGGTCCTCTTGCAAAAAATATTGCAGATCAATATGAAATTGATCCACGAAAATCGGCGAGTGTACTAGATGT
GTTCTCGTGTTGTGTTCAAGGGCTAGTTCCATACGGAGCGCAGATGTTAACAGCAGCAGGATTTGCGGCTTTATCACCAG
TTGAACTATTGCCGTATGCATTTTATCCAATTTTAGTTGGACTATGTGGTGTAGTCTCAATCTTAATTGATTTTCCGAGA
TTTTCAAAAGTCGAGGAAAAAGGACAATTTCATAAAACAGCCTAA

Upstream 100 bases:

>100_bases
TAATTGACTTTTGATTTATAAGCGAGTAAAGTTAGGAAAGACATTATTACAAATAAAAATATGAAACCTTCTTATAAAGA
GAGGCGGAGGGACTGGCCCT

Downstream 100 bases:

>100_bases
TTTTTTATTAGAATAAGGCAAAAAAGACGCTCATAAAAGTGATTTTGAGCGTCTTTTTATCGTTTATACGTCCTTTTTAG
TGGTTGTATTTCTCTTTTTT

Product: Na+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MMPRQRTRFWSAVPNPASMCLKDEKSVSYRCIRPLLLGRGLLLFSRKGLKLGRDDMVKETRGNIFALLPLGIFLVLFIGS
GIITGDFYKLPILVAILIAVGVALAMNRKESFTAKVERFAKGAGNPDIMIMVLIFVLAGAFSETAKGMGGVDSTVNLALA
VLPQGFIIAGIFVIGAFISLAMGTSMGTIAALAPIAVGISQQTDISIALAMATVVGGAMFGDNLSFISDTTIAAVRSQGT
EMKDKFKTNFLIVLPAAMITVILLVVLTLGNHTEIKTHAFDWMKILPYAGVLVTALLGWNVLVVLTGGTILSGIIGFLDG
SYTLASFFKSAATGMNGMMELVLLAILIGGMVEIIQYNGGIQYLMNTLTRNIRSKKGAEFGIAGLVSMTNICTANNTISI
IFTGPLAKNIADQYEIDPRKSASVLDVFSCCVQGLVPYGAQMLTAAGFAALSPVELLPYAFYPILVGLCGVVSILIDFPR
FSKVEEKGQFHKTA

Sequences:

>Translated_494_residues
MMPRQRTRFWSAVPNPASMCLKDEKSVSYRCIRPLLLGRGLLLFSRKGLKLGRDDMVKETRGNIFALLPLGIFLVLFIGS
GIITGDFYKLPILVAILIAVGVALAMNRKESFTAKVERFAKGAGNPDIMIMVLIFVLAGAFSETAKGMGGVDSTVNLALA
VLPQGFIIAGIFVIGAFISLAMGTSMGTIAALAPIAVGISQQTDISIALAMATVVGGAMFGDNLSFISDTTIAAVRSQGT
EMKDKFKTNFLIVLPAAMITVILLVVLTLGNHTEIKTHAFDWMKILPYAGVLVTALLGWNVLVVLTGGTILSGIIGFLDG
SYTLASFFKSAATGMNGMMELVLLAILIGGMVEIIQYNGGIQYLMNTLTRNIRSKKGAEFGIAGLVSMTNICTANNTISI
IFTGPLAKNIADQYEIDPRKSASVLDVFSCCVQGLVPYGAQMLTAAGFAALSPVELLPYAFYPILVGLCGVVSILIDFPR
FSKVEEKGQFHKTA
>Mature_494_residues
MMPRQRTRFWSAVPNPASMCLKDEKSVSYRCIRPLLLGRGLLLFSRKGLKLGRDDMVKETRGNIFALLPLGIFLVLFIGS
GIITGDFYKLPILVAILIAVGVALAMNRKESFTAKVERFAKGAGNPDIMIMVLIFVLAGAFSETAKGMGGVDSTVNLALA
VLPQGFIIAGIFVIGAFISLAMGTSMGTIAALAPIAVGISQQTDISIALAMATVVGGAMFGDNLSFISDTTIAAVRSQGT
EMKDKFKTNFLIVLPAAMITVILLVVLTLGNHTEIKTHAFDWMKILPYAGVLVTALLGWNVLVVLTGGTILSGIIGFLDG
SYTLASFFKSAATGMNGMMELVLLAILIGGMVEIIQYNGGIQYLMNTLTRNIRSKKGAEFGIAGLVSMTNICTANNTISI
IFTGPLAKNIADQYEIDPRKSASVLDVFSCCVQGLVPYGAQMLTAAGFAALSPVELLPYAFYPILVGLCGVVSILIDFPR
FSKVEEKGQFHKTA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 52628; Mature: 52628

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRQRTRFWSAVPNPASMCLKDEKSVSYRCIRPLLLGRGLLLFSRKGLKLGRDDMVKET
CCCCCHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHH
RGNIFALLPLGIFLVLFIGSGIITGDFYKLPILVAILIAVGVALAMNRKESFTAKVERFA
CCCEEEHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KGAGNPDIMIMVLIFVLAGAFSETAKGMGGVDSTVNLALAVLPQGFIIAGIFVIGAFISL
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMGTSMGTIAALAPIAVGISQQTDISIALAMATVVGGAMFGDNLSFISDTTIAAVRSQGT
HHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EMKDKFKTNFLIVLPAAMITVILLVVLTLGNHTEIKTHAFDWMKILPYAGVLVTALLGWN
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
VLVVLTGGTILSGIIGFLDGSYTLASFFKSAATGMNGMMELVLLAILIGGMVEIIQYNGG
EEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
IQYLMNTLTRNIRSKKGAEFGIAGLVSMTNICTANNTISIIFTGPLAKNIADQYEIDPRK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEEEEEECHHHHHHHHHCCCCCCC
SASVLDVFSCCVQGLVPYGAQMLTAAGFAALSPVELLPYAFYPILVGLCGVVSILIDFPR
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FSKVEEKGQFHKTA
HHHHHHCCCCCCCC
>Mature Secondary Structure
MMPRQRTRFWSAVPNPASMCLKDEKSVSYRCIRPLLLGRGLLLFSRKGLKLGRDDMVKET
CCCCCHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHH
RGNIFALLPLGIFLVLFIGSGIITGDFYKLPILVAILIAVGVALAMNRKESFTAKVERFA
CCCEEEHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KGAGNPDIMIMVLIFVLAGAFSETAKGMGGVDSTVNLALAVLPQGFIIAGIFVIGAFISL
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMGTSMGTIAALAPIAVGISQQTDISIALAMATVVGGAMFGDNLSFISDTTIAAVRSQGT
HHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EMKDKFKTNFLIVLPAAMITVILLVVLTLGNHTEIKTHAFDWMKILPYAGVLVTALLGWN
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
VLVVLTGGTILSGIIGFLDGSYTLASFFKSAATGMNGMMELVLLAILIGGMVEIIQYNGG
EEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
IQYLMNTLTRNIRSKKGAEFGIAGLVSMTNICTANNTISIIFTGPLAKNIADQYEIDPRK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEEEEEECHHHHHHHHHCCCCCCC
SASVLDVFSCCVQGLVPYGAQMLTAAGFAALSPVELLPYAFYPILVGLCGVVSILIDFPR
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FSKVEEKGQFHKTA
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]